MD16G1111500.v1.1

TRAM, LAG1 and CLN8 homology domains.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004398
Physical Location & Seq
Reverse (-)
7823663 .. 7824400
738 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1111500.v1.1.491

Sequence Viewer

Length: 738 bp
ATGTCGACCTCAGCACTCCCCACCCCAACACTCCCAACATTCTTCTCCATCTTTCTTCTCATCTACCTCTCTGCTTATTTCCTGCTTTTCCGAAACTGGGGCCCAAAACACCGACCAGAAGCTTCAAGCTGCCTAATCTCTTTATCCCACGGCTCCGCAGCTGCCGCCATGGCCGCCCACGCAATCCTCCGCAGCTACAAAGCCACCACCTTCGCCTCCCCCAACACCGACACCCAAAACACCGTGCTCGAGTTCAGCATCGCCTACTTCTCCCTCGACCTCCTCCACTACCTCCTCTTCTTCCCCAACGACGTCATCTTCATCCTCCACCACCTCGCCACCCTCTACGTCTTCACCACCTGCCGTTACGCCGTCCGCCACGGGGCCCACGCCATCCTCGTTCTCCTCCTCCTTGCTGAGATCACGAGCGGCTGCCAAAACGCCTGGACCCTTGCCGGGTTTCGGAGGGCCGGTTCGCCTGCCGCCGCCAAATTGTACGACTTTTTGTCCCCTAAATTCTATGCTTTTTACAGTGTGTTTAGAGGGATCCTGGGGCCGGTGTTCATGTTCAAGATGGGGGCGTTCTATGTGAGTGGGGCGGCTGACCCCGTCATCCCCAGATGGGCATGGGCTTCTTGGATGGTGGTGATTTCAATTGCCATTTGTCTTAGTATTTTGTGGGTTCTGAATCTTTGGATCGATTGGTACAAAAACAAAGTGCAGAAGAAAAAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

246

Amino Acids

27.58

Weight (kDa)

9.64

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 42 - 232 4.6e-15 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 368
AatII GACGTC 1 cut(s) 315
Acc36I ACCTGC 1 cut(s) 368
AccBSI CCGCTC 1 cut(s) 429
AccI GTMKAC 1 cut(s) 5
AciI CCGC 9 cut(s) 156, 165, 174, 190, 376, 429, 483, 486, 599
AclWI GGATC 3 cut(s) 541, 554, 704
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 515
AcyI GRCGYC 1 cut(s) 312
AfaI GTAC 2 cut(s) 497, 707
AfiI CCNNNNNNNGG 6 cut(s) 97, 382, 456, 462, 556, 622
AgsI TTSAA 3 cut(s) 126, 571, 654
AhdI GACNNNNNGTC 1 cut(s) 505
AjnI CCWGG 2 cut(s) 443, 549
AloI GAACNNNNNNTCC 2 cut(s) 457, 489
AluBI AGCT 4 cut(s) 122, 129, 161, 195
AluI AGCT 4 cut(s) 122, 129, 161, 195
Alw21I GWGCWC 1 cut(s) 249
AlwI GGATC 3 cut(s) 541, 554, 704
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 5 cut(s) 100, 171, 384, 468, 554
ApaI GGGCCC 2 cut(s) 104, 388
ApeKI GCWGC 5 cut(s) 129, 158, 161, 192, 432
ApoI RAATTY 1 cut(s) 515
AspS9I GGNCC 7 cut(s) 100, 101, 384, 385, 447, 468, 554
AsuC2I CCSGG 1 cut(s) 457
AsuHPI GGTGA 2 cut(s) 346, 658
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 1 cut(s) 447
BaeGI GKGCMC 2 cut(s) 104, 388
BamHI GGATCC 1 cut(s) 546
BanII GRGCYC 2 cut(s) 104, 388
BauI CACGAG 1 cut(s) 424
BbsI GAAGAC 1 cut(s) 343
Bbv12I GWGCWC 1 cut(s) 249
BbvCI CCTCAGC 1 cut(s) 10
BbvI GCAGC 5 cut(s) 116, 148, 170, 204, 419
BccI CCATC 5 cut(s) 56, 401, 568, 615, 634
BceAI ACGGC 3 cut(s) 166, 348, 356
BciT130I CCWGG 2 cut(s) 445, 551
BcnI CCSGG 1 cut(s) 457
BfuAI ACCTGC 1 cut(s) 368
BglI GCCNNNNNGGC 1 cut(s) 170
Bme1390I CCNGG 3 cut(s) 445, 457, 551
Bme18I GGWCC 1 cut(s) 447
BmeRI GACNNNNNGTC 1 cut(s) 505
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 7 cut(s) 100, 101, 384, 385, 447, 468, 554
BmiI GGNNCC 8 cut(s) 101, 102, 154, 385, 386, 449, 548, 555
BmrFI CCNGG 3 cut(s) 445, 457, 551
BmrI ACTGGG 1 cut(s) 106
BmsI GCATC 1 cut(s) 267
BmuI ACTGGG 1 cut(s) 106
BpiI GAAGAC 1 cut(s) 343
Bpu10I CCTNAGC 1 cut(s) 10
BpuMI CCSGG 1 cut(s) 457
Bsa29I ATCGAT 1 cut(s) 699
BsaHI GRCGYC 1 cut(s) 312
BsaJI CCNNGG 4 cut(s) 148, 168, 379, 550
BsaXI ACNNNNNCTCC 2 cut(s) 457, 487
Bsc4I CCNNNNNNNGG 6 cut(s) 97, 382, 456, 462, 556, 622
Bse118I RCCGGY 2 cut(s) 470, 556
Bse1I ACTGG 1 cut(s) 101
BseBI CCWGG 2 cut(s) 445, 551
BseCI ATCGAT 1 cut(s) 699
BseDI CCNNGG 4 cut(s) 148, 168, 379, 550
BseGI GGATG 4 cut(s) 321, 393, 612, 645
BseLI CCNNNNNNNGG 6 cut(s) 97, 382, 456, 462, 556, 622
BseMII CTCAG 2 cut(s) 24, 408
BseNI ACTGG 1 cut(s) 101
BseRI GAGGAG 4 cut(s) 272, 284, 395, 398
BseSI GKGCMC 2 cut(s) 104, 388
BseXI GCAGC 5 cut(s) 116, 148, 170, 204, 419
BshFI GGCC 5 cut(s) 102, 173, 386, 470, 556
BshVI ATCGAT 1 cut(s) 699
BsiHKAI GWGCWC 1 cut(s) 249
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 3 cut(s) 456, 471, 557
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 6 cut(s) 97, 382, 456, 462, 556, 622
BsmFI GGGAC 1 cut(s) 493
BsnI GGCC 5 cut(s) 102, 173, 386, 470, 556
BsoBI CYCGRG 1 cut(s) 248
Bsp120I GGGCCC 2 cut(s) 100, 384
Bsp1286I GDGCHC 3 cut(s) 104, 249, 388
Bsp143I GATC 3 cut(s) 420, 546, 696
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 9 cut(s) 156, 165, 174, 190, 376, 429, 483, 486, 599
BspANI GGCC 5 cut(s) 102, 173, 386, 470, 556
BspCNI CTCAG 2 cut(s) 23, 409
BspDI ATCGAT 1 cut(s) 699
BspLI GGNNCC 8 cut(s) 101, 102, 154, 385, 386, 449, 548, 555
BspMI ACCTGC 1 cut(s) 368
BspPI GGATC 3 cut(s) 541, 554, 704
BsrBI CCGCTC 1 cut(s) 429
BsrFI RCCGGY 2 cut(s) 470, 556
BsrI ACTGG 1 cut(s) 101
BssAI RCCGGY 2 cut(s) 470, 556
BssECI CCNNGG 4 cut(s) 148, 168, 379, 550
BssMI GATC 3 cut(s) 420, 546, 696
BssNI GRCGYC 1 cut(s) 312
BssSI CACGAG 1 cut(s) 424
BssT1I CCWWGG 1 cut(s) 168
Bst2BI CACGAG 1 cut(s) 424
Bst2UI CCWGG 2 cut(s) 445, 551
Bst4CI ACNGT 2 cut(s) 244, 533
Bst6I CTCTTC 1 cut(s) 302
BstACI GRCGYC 1 cut(s) 312
BstC8I GCNNGC 1 cut(s) 480
BstDEI CTNAG 3 cut(s) 10, 417, 668
BstDSI CCRYGG 3 cut(s) 148, 168, 379
BstF5I GGATG 4 cut(s) 321, 393, 612, 645
BstKTI GATC 3 cut(s) 423, 549, 699
BstMBI GATC 3 cut(s) 420, 546, 696
BstMWI GCNNNNNNNGC 4 cut(s) 164, 170, 173, 179
BstNI CCWGG 2 cut(s) 445, 551
BstSCI CCNGG 3 cut(s) 443, 455, 549
BstSLI GKGCMC 2 cut(s) 104, 388
BstV1I GCAGC 5 cut(s) 116, 148, 170, 204, 419
BstV2I GAAGAC 1 cut(s) 343
BstX2I RGATCY 1 cut(s) 546
BstYI RGATCY 1 cut(s) 546
Bsu15I ATCGAT 1 cut(s) 699
BsuRI GGCC 5 cut(s) 102, 173, 386, 470, 556
BsuTUI ATCGAT 1 cut(s) 699
BtgI CCRYGG 3 cut(s) 148, 168, 379
BtgZI GCGATG 1 cut(s) 244
BtsCI GGATG 4 cut(s) 321, 393, 612, 645
BtsIMutI CAGTG 1 cut(s) 538
BveI ACCTGC 1 cut(s) 368
Cac8I GCNNGC 1 cut(s) 480
Cfr10I RCCGGY 2 cut(s) 470, 556
Cfr13I GGNCC 7 cut(s) 100, 101, 384, 385, 447, 468, 554
ClaI ATCGAT 1 cut(s) 699
Csp6I GTAC 2 cut(s) 496, 706
CviAII CATG 3 cut(s) 169, 565, 627
CviQI GTAC 2 cut(s) 496, 706
DdeI CTNAG 3 cut(s) 10, 417, 668
DpnI GATC 3 cut(s) 422, 548, 698
DpnII GATC 3 cut(s) 420, 546, 696
DriI GACNNNNNGTC 1 cut(s) 505
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 302
Eam1105I GACNNNNNGTC 1 cut(s) 505
EarI CTCTTC 1 cut(s) 302
EciI GGCGGA 1 cut(s) 365
Eco130I CCWWGG 1 cut(s) 168
Eco24I GRGCYC 2 cut(s) 104, 388
Eco47I GGWCC 1 cut(s) 447
Eco88I CYCGRG 1 cut(s) 248
EcoO109I RGGNCCY 2 cut(s) 100, 384
EcoRII CCWGG 2 cut(s) 443, 549
EcoT14I CCWWGG 1 cut(s) 168
EcoT38I GRGCYC 2 cut(s) 104, 388
ErhI CCWWGG 1 cut(s) 168
FaeI CATG 3 cut(s) 172, 568, 630
FaiI YATR 5 cut(s) 170, 522, 566, 588, 628
FaqI GGGAC 1 cut(s) 493
FatI CATG 3 cut(s) 168, 564, 626
FblI GTMKAC 1 cut(s) 5
FokI GGATG 4 cut(s) 308, 380, 599, 652
FriOI GRGCYC 2 cut(s) 104, 388
HaeIII GGCC 5 cut(s) 102, 173, 386, 470, 556
HapII CCGG 3 cut(s) 456, 471, 557
Hin1I GRCGYC 1 cut(s) 312
Hin1II CATG 3 cut(s) 172, 568, 630
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 120
HinfI GANTC 1 cut(s) 688
HpaII CCGG 3 cut(s) 456, 471, 557
HphI GGTGA 2 cut(s) 346, 658
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 3 cut(s) 92, 465, 687
Hpy188III TCNNGA 2 cut(s) 424, 571
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 1 cut(s) 220
HpyCH4III ACNGT 2 cut(s) 244, 533
HpyCH4IV ACGT 2 cut(s) 312, 348
HpyCH4V TGCA 1 cut(s) 721
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 170, 173, 179
HpyF3I CTNAG 3 cut(s) 10, 417, 668
HpySE526I ACGT 2 cut(s) 312, 348
Hsp92I GRCGYC 1 cut(s) 312
Hsp92II CATG 3 cut(s) 172, 568, 630
Kzo9I GATC 3 cut(s) 420, 546, 696
LmnI GCTCC 1 cut(s) 158
Lsp1109I GCAGC 5 cut(s) 116, 148, 170, 204, 419
LweI GCATC 1 cut(s) 267
MaeII ACGT 2 cut(s) 312, 348
MaeIII GTNAC 1 cut(s) 365
MalI GATC 3 cut(s) 422, 548, 698
MbiI CCGCTC 1 cut(s) 429
MboI GATC 3 cut(s) 420, 546, 696
MboII GAAGA 7 cut(s) 34, 47, 289, 292, 310, 343, 736
MfeI CAATTG 1 cut(s) 654
MflI RGATCY 1 cut(s) 546
MhlI GDGCHC 3 cut(s) 104, 249, 388
MluCI AATT 3 cut(s) 491, 515, 654
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 3 cut(s) 456, 471, 557
MspR9I CCNGG 3 cut(s) 445, 457, 551
MunI CAATTG 1 cut(s) 654
MvaI CCWGG 2 cut(s) 445, 551
MwoI GCNNNNNNNGC 4 cut(s) 164, 170, 173, 179
NciI CCSGG 1 cut(s) 457
NcoI CCATGG 1 cut(s) 168
NdeII GATC 3 cut(s) 420, 546, 696
NlaIII CATG 3 cut(s) 172, 568, 630
NlaIV GGNNCC 8 cut(s) 101, 102, 154, 385, 386, 449, 548, 555
PaeR7I CTCGAG 1 cut(s) 248
PaqCI CACCTGC 1 cut(s) 368
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PfeI GAWTC 1 cut(s) 688
PflFI GACNNNGTC 1 cut(s) 608
Psp6I CCWGG 2 cut(s) 443, 549
PspGI CCWGG 2 cut(s) 443, 549
PspN4I GGNNCC 8 cut(s) 101, 102, 154, 385, 386, 449, 548, 555
PspOMI GGGCCC 2 cut(s) 100, 384
PspPI GGNCC 7 cut(s) 100, 101, 384, 385, 447, 468, 554
PspXI VCTCGAGB 1 cut(s) 248
PsuI RGATCY 1 cut(s) 546
PsyI GACNNNGTC 1 cut(s) 608
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 2 cut(s) 497, 707
RsaNI GTAC 2 cut(s) 496, 706
SalI GTCGAC 1 cut(s) 4
Sau3AI GATC 3 cut(s) 420, 546, 696
Sau96I GGNCC 7 cut(s) 100, 101, 384, 385, 447, 468, 554
ScrFI CCNGG 3 cut(s) 445, 457, 551
SduI GDGCHC 3 cut(s) 104, 249, 388
SfaNI GCATC 1 cut(s) 267
Sfr274I CTCGAG 1 cut(s) 248
SinI GGWCC 1 cut(s) 447
SlaI CTCGAG 1 cut(s) 248
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 3 cut(s) 491, 515, 654
SsiI CCGC 9 cut(s) 156, 165, 174, 190, 376, 429, 483, 486, 599
StyD4I CCNGG 3 cut(s) 443, 455, 549
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 2 cut(s) 244, 533
TaiI ACGT 2 cut(s) 315, 351
TaqI TCGA 4 cut(s) 5, 249, 276, 699
TasI AATT 3 cut(s) 491, 515, 654
TauI GCSGC 6 cut(s) 167, 176, 432, 485, 488, 602
TfiI GAWTC 1 cut(s) 688
TscAI CASTG 1 cut(s) 538
TseI GCWGC 5 cut(s) 129, 158, 161, 192, 432
TspDTI ATGAA 2 cut(s) 310, 553
TspRI CASTG 1 cut(s) 538
Tth111I GACNNNGTC 1 cut(s) 608
VpaK11BI GGWCC 1 cut(s) 447
XapI RAATTY 1 cut(s) 515
XhoI CTCGAG 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 5
ZraI GACGTC 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.