MD13G1111900.v1.1

TRAM, LAG1 and CLN8 homology domains.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004398
Physical Location & Seq
Reverse (-)
8137317 .. 8138054
738 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1111900.v1.1.491

Sequence Viewer

Length: 738 bp
ATGGAGACCTCAATTCTTCCCTCCCCAACACTTCCCACTTTCTTCTCCATCTTTCTCCTCATCTACCTCTATGCATACTTCTTACTCTTCCGCAACTGGGGCCCAAAACACCGACCAGAAGCATCAAGCTGCCTCATCTCTCTATCCCACGGCACCGCCGCTGCCGCCATGGCCGCCCACGCAATCCTCCGCAGCCACAAAGCCACAACCTTCGCCTCCCCCAACACCCCCACCCAAAACACCGTGCTCGAATTCAGCATCGCCTACTTCTCCCTCGACCTCCTCCACTACCTCCTCTTCTTCCCCAACGACGTCCTCTTCATCCTCCACCACCTCGCCACCCTCTACGTCTTCACCACCTGCCTTTACGTCGTCCGCCACGGGGCCCACGCCATCCTTGTTCTTCTCTTCCTTGCTGAGATCACCAGCGGCTGCCAGAACGCCTGGACCCTCGCCGATTTTCGGAGGGCCGGTTCGCCCGCCGCGGCCAAATTGTACGACTTTTTGTCCCCTAAATTCTATGCCTTGTACAGTGTTTTTAGAGGGATTTTAGGGCCAATGTTCATGTACAGGATGGCGGTGTTCTACGTGAGCGGGGCGGCTGACCCCGTGATCCCCAGATGGGCATGGGTTTCTTGGATGGTTGTGATTTCAGTTGCCATTTGTCTTAGTATTTTGTGGGTTTTGAATCTTTGGATCGATTGGTACAAAAACAAAGCGCAAAAGAAAAAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

246

Amino Acids

27.85

Weight (kDa)

9.47

Isoelectric Point (pI)

47.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 42 - 232 2.6e-18 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 368
AatII GACGTC 1 cut(s) 315
Acc36I ACCTGC 1 cut(s) 368
AccB1I GGYRCC 1 cut(s) 152
AccBSI CCGCTC 1 cut(s) 594
AccII CGCG 1 cut(s) 485
AclWI GGATC 2 cut(s) 607, 704
AcoI YGGCCR 2 cut(s) 171, 486
AcsI RAATTY 2 cut(s) 251, 515
AcyI GRCGYC 1 cut(s) 312
AfaI GTAC 4 cut(s) 497, 530, 569, 707
AfiI CCNNNNNNNGG 4 cut(s) 97, 382, 462, 622
AgsI TTSAA 1 cut(s) 688
AhdI GACNNNNNGTC 1 cut(s) 505
AjnI CCWGG 1 cut(s) 443
AloI GAACNNNNNNTCC 2 cut(s) 457, 489
AluBI AGCT 1 cut(s) 129
AluI AGCT 1 cut(s) 129
Alw21I GWGCWC 1 cut(s) 249
AlwI GGATC 2 cut(s) 607, 704
AlwNI CAGNNNCTG 1 cut(s) 432
AoxI GGCC 6 cut(s) 100, 171, 384, 468, 486, 554
ApaI GGGCCC 2 cut(s) 104, 388
ApeKI GCWGC 4 cut(s) 129, 161, 192, 432
ApoI RAATTY 2 cut(s) 251, 515
AspLEI GCGC 1 cut(s) 721
AspS9I GGNCC 7 cut(s) 100, 101, 384, 385, 447, 468, 554
AsuHPI GGTGA 2 cut(s) 346, 415
AvaII GGWCC 1 cut(s) 447
BaeGI GKGCMC 2 cut(s) 104, 388
BanI GGYRCC 1 cut(s) 152
BanII GRGCYC 2 cut(s) 104, 388
BbsI GAAGAC 1 cut(s) 343
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 4 cut(s) 116, 148, 204, 419
BccI CCATC 5 cut(s) 56, 401, 568, 615, 634
BceAI ACGGC 1 cut(s) 166
BciT130I CCWGG 1 cut(s) 445
BfuAI ACCTGC 1 cut(s) 368
BglI GCCNNNNNGGC 1 cut(s) 170
Bme1390I CCNGG 1 cut(s) 445
Bme18I GGWCC 1 cut(s) 447
BmeRI GACNNNNNGTC 1 cut(s) 505
BmgT120I GGNCC 7 cut(s) 100, 101, 384, 385, 447, 468, 554
BmiI GGNNCC 6 cut(s) 101, 102, 154, 385, 386, 449
BmrFI CCNGG 1 cut(s) 445
BmrI ACTGGG 1 cut(s) 106
BmsI GCATC 2 cut(s) 131, 267
BmuI ACTGGG 1 cut(s) 106
BpiI GAAGAC 1 cut(s) 343
Bsa29I ATCGAT 1 cut(s) 699
BsaAI YACGTR 1 cut(s) 589
BsaHI GRCGYC 1 cut(s) 312
BsaJI CCNNGG 4 cut(s) 148, 168, 379, 483
BsaXI ACNNNNNCTCC 2 cut(s) 457, 487
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 382, 462, 622
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 1 cut(s) 101
BseBI CCWGG 1 cut(s) 445
BseCI ATCGAT 1 cut(s) 699
BseDI CCNNGG 4 cut(s) 148, 168, 379, 483
BseGI GGATG 4 cut(s) 321, 393, 579, 645
BseLI CCNNNNNNNGG 4 cut(s) 97, 382, 462, 622
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 1 cut(s) 101
BseRI GAGGAG 3 cut(s) 47, 272, 284
BseSI GKGCMC 2 cut(s) 104, 388
BseXI GCAGC 4 cut(s) 116, 148, 204, 419
Bsh1236I CGCG 1 cut(s) 485
BshFI GGCC 6 cut(s) 102, 173, 386, 470, 488, 556
BshNI GGYRCC 1 cut(s) 152
BshVI ATCGAT 1 cut(s) 699
BsiHKAI GWGCWC 1 cut(s) 249
BsiSI CCGG 1 cut(s) 471
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 4 cut(s) 97, 382, 462, 622
BsmFI GGGAC 1 cut(s) 493
BsnI GGCC 6 cut(s) 102, 173, 386, 470, 488, 556
Bsp120I GGGCCC 2 cut(s) 100, 384
Bsp1286I GDGCHC 3 cut(s) 104, 249, 388
Bsp1407I TGTACA 2 cut(s) 528, 567
Bsp143I GATC 3 cut(s) 420, 612, 696
Bsp19I CCATGG 1 cut(s) 168
BspANI GGCC 6 cut(s) 102, 173, 386, 470, 488, 556
BspCNI CTCAG 1 cut(s) 409
BspDI ATCGAT 1 cut(s) 699
BspFNI CGCG 1 cut(s) 485
BspLI GGNNCC 6 cut(s) 101, 102, 154, 385, 386, 449
BspMI ACCTGC 1 cut(s) 368
BspPI GGATC 2 cut(s) 607, 704
BspT107I GGYRCC 1 cut(s) 152
BsrBI CCGCTC 1 cut(s) 594
BsrFI RCCGGY 1 cut(s) 470
BsrGI TGTACA 2 cut(s) 528, 567
BsrI ACTGG 1 cut(s) 101
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 4 cut(s) 148, 168, 379, 483
BssMI GATC 3 cut(s) 420, 612, 696
BssNI GRCGYC 1 cut(s) 312
BssT1I CCWWGG 1 cut(s) 168
Bst2UI CCWGG 1 cut(s) 445
Bst4CI ACNGT 2 cut(s) 244, 533
Bst6I CTCTTC 4 cut(s) 92, 302, 323, 413
BstACI GRCGYC 1 cut(s) 312
BstAUI TGTACA 2 cut(s) 528, 567
BstBAI YACGTR 1 cut(s) 589
BstC8I GCNNGC 1 cut(s) 480
BstDEI CTNAG 2 cut(s) 417, 668
BstDSI CCRYGG 4 cut(s) 148, 168, 379, 483
BstF5I GGATG 4 cut(s) 321, 393, 579, 645
BstFNI CGCG 1 cut(s) 485
BstHHI GCGC 1 cut(s) 721
BstKTI GATC 3 cut(s) 423, 615, 699
BstMBI GATC 3 cut(s) 420, 612, 696
BstMWI GCNNNNNNNGC 5 cut(s) 99, 164, 170, 173, 179
BstNI CCWGG 1 cut(s) 445
BstSCI CCNGG 1 cut(s) 443
BstSLI GKGCMC 2 cut(s) 104, 388
BstUI CGCG 1 cut(s) 485
BstV1I GCAGC 4 cut(s) 116, 148, 204, 419
BstV2I GAAGAC 1 cut(s) 343
Bsu15I ATCGAT 1 cut(s) 699
BsuRI GGCC 6 cut(s) 102, 173, 386, 470, 488, 556
BsuTUI ATCGAT 1 cut(s) 699
BtgI CCRYGG 4 cut(s) 148, 168, 379, 483
BtgZI GCGATG 1 cut(s) 244
BtsCI GGATG 4 cut(s) 321, 393, 579, 645
BtsIMutI CAGTG 1 cut(s) 538
BveI ACCTGC 1 cut(s) 368
Cac8I GCNNGC 1 cut(s) 480
CaiI CAGNNNCTG 1 cut(s) 432
CfoI GCGC 1 cut(s) 721
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 7 cut(s) 100, 101, 384, 385, 447, 468, 554
Cfr42I CCGCGG 1 cut(s) 486
ClaI ATCGAT 1 cut(s) 699
Csp6I GTAC 4 cut(s) 496, 529, 568, 706
CviAII CATG 3 cut(s) 169, 565, 627
CviQI GTAC 4 cut(s) 496, 529, 568, 706
DdeI CTNAG 2 cut(s) 417, 668
DpnI GATC 3 cut(s) 422, 614, 698
DpnII GATC 3 cut(s) 420, 612, 696
DriI GACNNNNNGTC 1 cut(s) 505
EaeI YGGCCR 2 cut(s) 171, 486
Eam1104I CTCTTC 4 cut(s) 92, 302, 323, 413
Eam1105I GACNNNNNGTC 1 cut(s) 505
EarI CTCTTC 4 cut(s) 92, 302, 323, 413
EciI GGCGGA 1 cut(s) 365
Eco130I CCWWGG 1 cut(s) 168
Eco24I GRGCYC 2 cut(s) 104, 388
Eco47I GGWCC 1 cut(s) 447
EcoO109I RGGNCCY 2 cut(s) 100, 384
EcoRI GAATTC 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 443
EcoT14I CCWWGG 1 cut(s) 168
EcoT22I ATGCAT 1 cut(s) 76
EcoT38I GRGCYC 2 cut(s) 104, 388
ErhI CCWWGG 1 cut(s) 168
FaeI CATG 3 cut(s) 172, 568, 630
FaiI YATR 6 cut(s) 72, 76, 170, 522, 566, 628
FaqI GGGAC 1 cut(s) 493
FatI CATG 3 cut(s) 168, 564, 626
FauI CCCGC 2 cut(s) 487, 587
FokI GGATG 4 cut(s) 308, 380, 586, 652
FriOI GRGCYC 2 cut(s) 104, 388
GlaI GCGC 1 cut(s) 720
HaeIII GGCC 6 cut(s) 102, 173, 386, 470, 488, 556
HapII CCGG 1 cut(s) 471
HhaI GCGC 1 cut(s) 721
Hin1I GRCGYC 1 cut(s) 312
Hin1II CATG 3 cut(s) 172, 568, 630
Hin6I GCGC 1 cut(s) 719
HinP1I GCGC 1 cut(s) 719
HinfI GANTC 1 cut(s) 688
HpaII CCGG 1 cut(s) 471
HphI GGTGA 2 cut(s) 346, 415
Hpy188I TCNGA 1 cut(s) 465
Hpy99I CGWCG 2 cut(s) 314, 374
HpyAV CCTTC 1 cut(s) 220
HpyCH4III ACNGT 2 cut(s) 244, 533
HpyCH4IV ACGT 4 cut(s) 312, 348, 369, 588
HpyCH4V TGCA 1 cut(s) 74
HpyF10VI GCNNNNNNNGC 5 cut(s) 99, 164, 170, 173, 179
HpyF3I CTNAG 2 cut(s) 417, 668
HpySE526I ACGT 4 cut(s) 312, 348, 369, 588
Hsp92I GRCGYC 1 cut(s) 312
Hsp92II CATG 3 cut(s) 172, 568, 630
HspAI GCGC 1 cut(s) 719
KspI CCGCGG 1 cut(s) 486
Kzo9I GATC 3 cut(s) 420, 612, 696
Lsp1109I GCAGC 4 cut(s) 116, 148, 204, 419
LweI GCATC 2 cut(s) 131, 267
MaeII ACGT 4 cut(s) 312, 348, 369, 588
MalI GATC 3 cut(s) 422, 614, 698
MbiI CCGCTC 1 cut(s) 594
MboI GATC 3 cut(s) 420, 612, 696
MboII GAAGA 9 cut(s) 8, 34, 79, 289, 292, 310, 343, 395, 400
MhlI GDGCHC 3 cut(s) 104, 249, 388
MluCI AATT 4 cut(s) 12, 251, 491, 515
Mph1103I ATGCAT 1 cut(s) 76
MspA1I CMGCKG 3 cut(s) 161, 429, 485
MspI CCGG 1 cut(s) 471
MspR9I CCNGG 1 cut(s) 445
MvaI CCWGG 1 cut(s) 445
MvnI CGCG 1 cut(s) 485
MwoI GCNNNNNNNGC 5 cut(s) 99, 164, 170, 173, 179
NcoI CCATGG 1 cut(s) 168
NdeII GATC 3 cut(s) 420, 612, 696
NlaIII CATG 3 cut(s) 172, 568, 630
NlaIV GGNNCC 6 cut(s) 101, 102, 154, 385, 386, 449
NsiI ATGCAT 1 cut(s) 76
PaqCI CACCTGC 1 cut(s) 368
PfeI GAWTC 1 cut(s) 688
Ppu21I YACGTR 1 cut(s) 589
Psp6I CCWGG 1 cut(s) 443
PspGI CCWGG 1 cut(s) 443
PspN4I GGNNCC 6 cut(s) 101, 102, 154, 385, 386, 449
PspOMI GGGCCC 2 cut(s) 100, 384
PspPI GGNCC 7 cut(s) 100, 101, 384, 385, 447, 468, 554
PstNI CAGNNNCTG 1 cut(s) 432
RsaI GTAC 4 cut(s) 497, 530, 569, 707
RsaNI GTAC 4 cut(s) 496, 529, 568, 706
SacII CCGCGG 1 cut(s) 486
Sau3AI GATC 3 cut(s) 420, 612, 696
Sau96I GGNCC 7 cut(s) 100, 101, 384, 385, 447, 468, 554
ScrFI CCNGG 1 cut(s) 445
SduI GDGCHC 3 cut(s) 104, 249, 388
SfaNI GCATC 2 cut(s) 131, 267
Sfr303I CCGCGG 1 cut(s) 486
SgrBI CCGCGG 1 cut(s) 486
SinI GGWCC 1 cut(s) 447
Sse9I AATT 4 cut(s) 12, 251, 491, 515
StyD4I CCNGG 1 cut(s) 443
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 2 cut(s) 244, 533
TaiI ACGT 4 cut(s) 315, 351, 372, 591
TaqI TCGA 3 cut(s) 249, 276, 699
TasI AATT 4 cut(s) 12, 251, 491, 515
TatI WGTACW 2 cut(s) 528, 567
TauI GCSGC 7 cut(s) 161, 167, 176, 432, 485, 488, 602
TfiI GAWTC 1 cut(s) 688
TscAI CASTG 1 cut(s) 538
TseI GCWGC 4 cut(s) 129, 161, 192, 432
TspDTI ATGAA 2 cut(s) 310, 553
TspRI CASTG 1 cut(s) 538
VpaK11BI GGWCC 1 cut(s) 447
XapI RAATTY 2 cut(s) 251, 515
ZraI GACGTC 1 cut(s) 313
Zsp2I ATGCAT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.