RLG00000006919

TRAM, LAG1 and CLN8 homology domains.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004398
Physical Location & Seq
Reverse (-)
11876435 .. 11877172
738 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006919

Sequence Viewer

Length: 738 bp
ATGGAGACCCCAGTTCTTTTCACCCCAACACTCCCCACATTCTTCACCATGTTCTTGCTCATCTATCTCTTCGCTAGATTCGTGCTTTTTCGAAGCTGGGGTCCCAAACATCGGCCAGAAGCTTCCAGCTGTGTAATATCTCTAGCTCATAGCACTCCTGCAGTTTTCATGGCGATCCATGCACTAATTCATAGCCAGACAACTTCCATTTTCGCCTCCCAAAACACAGCTTTCGAAAACACTGTGCTTGAATACAGCATAGCTTACTTCTTGGTTGACCTTCTTCACTACTTGCTCTTCTTCCCTACTGAAGTCCTCTTCATTCTTCACCACCTGGCCACACTGTACGTGTTTTTGACTTGCCGCTATGTGGTTCATCATGGGGCTTATGCCATCATCGTGCTTCTTTTTCTTGCTGAGATCACCAGCGGTTGCCAGAACGTGTGGACACTTGCGAGTTTTCGAAGGGATGATTCGGCTTCTGCTGCGAAACTGTATGACTTCTTGTCTCCTCGGTTTTATGCTTTTTACACTGTTTTTAGAGGGGTTCTGGGGCCTCTGTTTATGTTTAAGATGGGGTTGTTCTATGCGAGTGGGGTGGCCAGAGAAGAAGTTCCGACATGGGCGTGGGTTTCTTGGATGGTTGTGATTGTAATAGCTATCTGTGTTAGCATATTGTGGGTTTTGAGTCATTGGAAAGATTGGTACAGAGACAAACTTCAGAAGAAAGAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

246

Amino Acids

28.39

Weight (kDa)

8.65

Isoelectric Point (pI)

35.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 42 - 230 7.1e-17 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 364, 429
AclWI GGATC 1 cut(s) 169
AcoI YGGCCR 3 cut(s) 113, 336, 600
AcuI CTGAAG 2 cut(s) 330, 704
AfaI GTAC 2 cut(s) 347, 707
AfiI CCNNNNNNNGG 2 cut(s) 111, 370
AflIII ACRYGT 2 cut(s) 348, 441
AgsI TTSAA 1 cut(s) 251
AhdI GACNNNNNGTC 1 cut(s) 505
AjnI CCWGG 1 cut(s) 333
AluBI AGCT 7 cut(s) 96, 122, 129, 146, 230, 263, 659
AluI AGCT 7 cut(s) 96, 122, 129, 146, 230, 263, 659
Alw26I GTCTC 2 cut(s) 513, 705
AlwI GGATC 1 cut(s) 169
AoxI GGCC 4 cut(s) 113, 336, 554, 600
ApeKI GCWGC 1 cut(s) 485
ArsI GACNNNNNNTTYG 2 cut(s) 85, 117
Asp700I GAANNNNTTC 1 cut(s) 612
AspS9I GGNCC 2 cut(s) 101, 554
AsuHPI GGTGA 4 cut(s) 13, 37, 320, 415
AsuII TTCGAA 3 cut(s) 91, 234, 463
AvaII GGWCC 1 cut(s) 101
BalI TGGCCA 2 cut(s) 338, 602
BbvI GCAGC 1 cut(s) 472
BccI CCATC 3 cut(s) 401, 568, 634
BciT130I CCWGG 1 cut(s) 335
BcoDI GTCTC 2 cut(s) 513, 705
BfaI CTAG 2 cut(s) 75, 143
BfmI CTRYAG 1 cut(s) 159
BisI GCNGC 2 cut(s) 364, 486
BlsI GCNGC 2 cut(s) 365, 487
Bme1390I CCNGG 1 cut(s) 335
Bme18I GGWCC 1 cut(s) 101
BmeRI GACNNNNNGTC 1 cut(s) 505
BmgT120I GGNCC 2 cut(s) 101, 554
BmiI GGNNCC 3 cut(s) 102, 103, 555
BmrFI CCNGG 1 cut(s) 335
BmrI ACTGGG 1 cut(s) 5
BmuI ACTGGG 1 cut(s) 5
Bpu14I TTCGAA 3 cut(s) 91, 234, 463
BsaAI YACGTR 1 cut(s) 349
BsaJI CCNNGG 1 cut(s) 512
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 370
Bse1I ACTGG 1 cut(s) 11
BseBI CCWGG 1 cut(s) 335
BseDI CCNNGG 1 cut(s) 512
BseGI GGATG 2 cut(s) 475, 645
BseLI CCNNNNNNNGG 2 cut(s) 111, 370
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 1 cut(s) 11
BseRI GAGGAG 1 cut(s) 501
BseXI GCAGC 1 cut(s) 472
BseYI CCCAGC 1 cut(s) 96
BshFI GGCC 4 cut(s) 115, 338, 556, 602
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 2 cut(s) 111, 370
BsmAI GTCTC 2 cut(s) 513, 705
BsmFI GGGAC 1 cut(s) 87
BsnI GGCC 4 cut(s) 115, 338, 556, 602
Bsp119I TTCGAA 3 cut(s) 91, 234, 463
Bsp143I GATC 2 cut(s) 174, 420
BspACI CCGC 2 cut(s) 364, 429
BspANI GGCC 4 cut(s) 115, 338, 556, 602
BspCNI CTCAG 1 cut(s) 409
BspLI GGNNCC 3 cut(s) 102, 103, 555
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 1 cut(s) 169
BspQI GCTCTTC 1 cut(s) 302
BspT104I TTCGAA 3 cut(s) 91, 234, 463
BsrI ACTGG 1 cut(s) 11
BssECI CCNNGG 1 cut(s) 512
BssMI GATC 2 cut(s) 174, 420
Bst2UI CCWGG 1 cut(s) 335
Bst4CI ACNGT 4 cut(s) 244, 345, 495, 535
Bst6I CTCTTC 3 cut(s) 74, 302, 323
BstBAI YACGTR 1 cut(s) 349
BstBI TTCGAA 3 cut(s) 91, 234, 463
BstDEI CTNAG 1 cut(s) 417
BstF5I GGATG 2 cut(s) 475, 645
BstKTI GATC 2 cut(s) 177, 423
BstMAI GTCTC 2 cut(s) 513, 705
BstMBI GATC 2 cut(s) 174, 420
BstMWI GCNNNNNNNGC 2 cut(s) 179, 485
BstNI CCWGG 1 cut(s) 335
BstSCI CCNGG 1 cut(s) 333
BstSFI CTRYAG 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 472
BsuRI GGCC 4 cut(s) 115, 338, 556, 602
BtsCI GGATG 2 cut(s) 475, 645
BtsIMutI CAGTG 3 cut(s) 240, 341, 531
Cfr13I GGNCC 2 cut(s) 101, 554
Csp6I GTAC 2 cut(s) 346, 706
CviAII CATG 5 cut(s) 49, 169, 179, 380, 621
CviQI GTAC 2 cut(s) 346, 706
DdeI CTNAG 1 cut(s) 417
DpnI GATC 2 cut(s) 176, 422
DpnII GATC 2 cut(s) 174, 420
DriI GACNNNNNGTC 1 cut(s) 505
EaeI YGGCCR 3 cut(s) 113, 336, 600
Eam1104I CTCTTC 3 cut(s) 74, 302, 323
Eam1105I GACNNNNNGTC 1 cut(s) 505
EarI CTCTTC 3 cut(s) 74, 302, 323
Eco47I GGWCC 1 cut(s) 101
Eco57I CTGAAG 2 cut(s) 330, 704
EcoO109I RGGNCCY 2 cut(s) 101, 554
EcoRII CCWGG 1 cut(s) 333
FaeI CATG 5 cut(s) 52, 172, 182, 383, 624
FaqI GGGAC 1 cut(s) 87
FatI CATG 5 cut(s) 48, 168, 178, 379, 620
Fnu4HI GCNGC 2 cut(s) 364, 486
FokI GGATG 2 cut(s) 482, 652
Fsp4HI GCNGC 2 cut(s) 364, 486
FspBI CTAG 2 cut(s) 75, 143
GluI GCNGC 2 cut(s) 364, 486
GsaI CCCAGC 1 cut(s) 100
HaeIII GGCC 4 cut(s) 115, 338, 556, 602
Hin1II CATG 5 cut(s) 52, 172, 182, 383, 624
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 120
HinfI GANTC 3 cut(s) 78, 473, 688
HphI GGTGA 4 cut(s) 13, 37, 320, 415
Hpy166II GTNNAC 2 cut(s) 277, 447
Hpy188I TCNGA 2 cut(s) 618, 723
Hpy8I GTNNAC 2 cut(s) 277, 447
HpyAV CCTTC 2 cut(s) 290, 459
HpyCH4III ACNGT 4 cut(s) 244, 345, 495, 535
HpyCH4IV ACGT 2 cut(s) 348, 441
HpyCH4V TGCA 2 cut(s) 161, 182
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 485
HpyF3I CTNAG 1 cut(s) 417
HpySE526I ACGT 2 cut(s) 348, 441
Hsp92II CATG 5 cut(s) 52, 172, 182, 383, 624
KflI GGGWCCC 1 cut(s) 101
Kzo9I GATC 2 cut(s) 174, 420
LguI GCTCTTC 1 cut(s) 302
Lsp1109I GCAGC 1 cut(s) 472
MaeI CTAG 2 cut(s) 75, 143
MaeII ACGT 2 cut(s) 348, 441
MalI GATC 2 cut(s) 176, 422
MboI GATC 2 cut(s) 174, 420
MboII GAAGA 9 cut(s) 34, 61, 275, 289, 292, 310, 317, 620, 736
MlsI TGGCCA 2 cut(s) 338, 602
MluCI AATT 1 cut(s) 186
MluNI TGGCCA 2 cut(s) 338, 602
MlyI GAGTC 1 cut(s) 697
MmeI TCCRAC 1 cut(s) 641
MnlI CCTC 6 cut(s) 226, 326, 522, 536, 567, 726
Mox20I TGGCCA 2 cut(s) 338, 602
MroXI GAANNNNTTC 1 cut(s) 612
MscI TGGCCA 2 cut(s) 338, 602
MseI TTAA 1 cut(s) 570
MslI CAYNNNNRTG 2 cut(s) 398, 625
Msp20I TGGCCA 2 cut(s) 338, 602
MspA1I CMGCKG 2 cut(s) 129, 429
MspR9I CCNGG 1 cut(s) 335
MvaI CCWGG 1 cut(s) 335
MwoI GCNNNNNNNGC 2 cut(s) 179, 485
NdeII GATC 2 cut(s) 174, 420
NlaIII CATG 5 cut(s) 52, 172, 182, 383, 624
NlaIV GGNNCC 3 cut(s) 102, 103, 555
NspV TTCGAA 3 cut(s) 91, 234, 463
PciSI GCTCTTC 1 cut(s) 302
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 612
PfeI GAWTC 2 cut(s) 78, 473
PkrI GCNGC 2 cut(s) 365, 487
PleI GAGTC 1 cut(s) 696
PpsI GAGTC 1 cut(s) 696
Ppu21I YACGTR 1 cut(s) 349
PpuMI RGGWCCY 1 cut(s) 101
Psp5II RGGWCCY 1 cut(s) 101
Psp6I CCWGG 1 cut(s) 333
PspFI CCCAGC 1 cut(s) 96
PspGI CCWGG 1 cut(s) 333
PspN4I GGNNCC 3 cut(s) 102, 103, 555
PspPI GGNCC 2 cut(s) 101, 554
PspPPI RGGWCCY 1 cut(s) 101
PstI CTGCAG 1 cut(s) 163
PvuII CAGCTG 1 cut(s) 129
RsaI GTAC 2 cut(s) 347, 707
RsaNI GTAC 2 cut(s) 346, 706
RseI CAYNNNNRTG 2 cut(s) 398, 625
SapI GCTCTTC 1 cut(s) 302
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 2 cut(s) 364, 486
Sau3AI GATC 2 cut(s) 174, 420
Sau96I GGNCC 2 cut(s) 101, 554
SchI GAGTC 1 cut(s) 697
ScrFI CCNGG 1 cut(s) 335
SfcI CTRYAG 1 cut(s) 159
SfuI TTCGAA 3 cut(s) 91, 234, 463
SinI GGWCC 1 cut(s) 101
SmiMI CAYNNNNRTG 2 cut(s) 398, 625
Sse9I AATT 1 cut(s) 186
SsiI CCGC 2 cut(s) 364, 429
SspMI CTAG 2 cut(s) 75, 143
StyD4I CCNGG 1 cut(s) 333
TaaI ACNGT 4 cut(s) 244, 345, 495, 535
TaiI ACGT 2 cut(s) 351, 444
TaqI TCGA 3 cut(s) 91, 234, 463
TasI AATT 1 cut(s) 186
TauI GCSGC 1 cut(s) 366
TfiI GAWTC 2 cut(s) 78, 473
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TscAI CASTG 3 cut(s) 247, 348, 538
TseI GCWGC 1 cut(s) 485
TspDTI ATGAA 4 cut(s) 157, 179, 310, 365
TspRI CASTG 3 cut(s) 247, 348, 538
VpaK11BI GGWCC 1 cut(s) 101
XmnI GAANNNNTTC 1 cut(s) 612
XspI CTAG 2 cut(s) 75, 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.