MD09G1016200.v1.1

TRAM, LAG1 and CLN8 homology domains.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004398
Physical Location & Seq
Forward (+)
997482 .. 999133
1652 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1016200.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGAATTGGAAACCCAAAACCCAATCCACTTCCCCCTTCCATGCCTCCCCATCTTCTTCCTCATGTTCTCCATCATCTACCTCATTGCCTACTTCATCGTCTTACGAACTTGGAGCCCCAAGATCCGACCCGAAGCCTCCAGCTGCCTAATCTCCCTCGCCCACGGCACCCCCGCCGTACTCCTCTCCACTTACGCCATCCTCTCCGACCCCGCCACCGGATTCGCCGCCCCCAACACCCTTTTCCAGAACTCCGTCCTCGACTACAGCGTCGCCTACTTCCTCACCGATCTCCTCCACTACCTCGTCTTCTTCCCCAGCGACGTCCTCTTCATCGGCCACCACCTTGCCACGCTCTTCGTCTTCCTCACCTGCCGCTACGTCGCCTCCCACGGCGCATTCGCCATTCTCTCCCTCCTGATCCTCGCCGAGGTCACCAGCCTCTGCCAGAACGTCTGGACGCTCGCCAACGCCAGGAGAAGCGACTTGAAGTTTGCAGCTAAAGTGTATGATCTTTTGTCTCCTCCATTTTACATTTTGTATTCGATTGTCCGTGGCTTTGTGGGTCCGTATTTCGTGTACCAGATGGGGGCGTTTTACATCAGCGGCGCCGCCGACGGATTGATTCCGCGGTGGGTTTGGGTTTCTTGGATTGTGGTGGTGGTGGCCGCCATTTCTGTCAGCATTTTGTGGATTTCCAATCTCTGGGTGGAATTGTTTAGAGCCCGGACCGGCGAATTGGAAAAGAAAACTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573 TRAM_LAG1_CLN8

Protein Analysis

253

Amino Acids

28.42

Weight (kDa)

7.7

Isoelectric Point (pI)

33.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 47 - 236 6.7e-18 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 380
AasI GACNNNNNNGTC 1 cut(s) 269
AatII GACGTC 1 cut(s) 327
Acc36I ACCTGC 1 cut(s) 380
AccB1I GGYRCC 2 cut(s) 167, 608
AccB7I CCANNNNNTGG 1 cut(s) 705
AccII CGCG 1 cut(s) 631
AciI CCGC 9 cut(s) 174, 213, 228, 376, 606, 612, 629, 631, 669
AclWI GGATC 2 cut(s) 118, 415
AcoI YGGCCR 2 cut(s) 337, 666
AcyI GRCGYC 2 cut(s) 324, 609
AfaI GTAC 2 cut(s) 180, 581
AfiI CCNNNNNNNGG 5 cut(s) 218, 430, 474, 589, 705
AgsI TTSAA 1 cut(s) 490
AjnI CCWGG 1 cut(s) 473
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AluBI AGCT 2 cut(s) 144, 500
AluI AGCT 2 cut(s) 144, 500
Alw26I GTCTC 1 cut(s) 525
AlwI GGATC 2 cut(s) 118, 415
AlwNI CAGNNNCTG 1 cut(s) 444
AoxI GGCC 2 cut(s) 337, 666
ApeKI GCWGC 2 cut(s) 144, 497
ArsI GACNNNNNNTTYG 2 cut(s) 476, 508
AspLEI GCGC 2 cut(s) 398, 611
AspS9I GGNCC 2 cut(s) 566, 729
AsuC2I CCSGG 1 cut(s) 727
AsuHPI GGTGA 3 cut(s) 277, 361, 427
AvaII GGWCC 2 cut(s) 566, 729
BanI GGYRCC 2 cut(s) 167, 608
BanII GRGCYC 2 cut(s) 119, 727
BbsI GAAGAC 2 cut(s) 301, 355
BbvI GCAGC 2 cut(s) 131, 509
BccI CCATC 4 cut(s) 59, 80, 206, 580
BceAI ACGGC 3 cut(s) 161, 181, 409
BciT130I CCWGG 1 cut(s) 475
BcnI CCSGG 1 cut(s) 727
BcoDI GTCTC 1 cut(s) 525
BfaI CTAG 1 cut(s) 753
BfmI CTRYAG 1 cut(s) 265
BfoI RGCGCY 1 cut(s) 612
BfuAI ACCTGC 1 cut(s) 380
BisI GCNGC 7 cut(s) 145, 228, 376, 498, 607, 612, 669
BlsI GCNGC 7 cut(s) 146, 229, 377, 499, 608, 613, 670
Bme1390I CCNGG 2 cut(s) 475, 727
Bme18I GGWCC 2 cut(s) 566, 729
BmgT120I GGNCC 2 cut(s) 566, 729
BmiI GGNNCC 4 cut(s) 116, 169, 567, 610
BmrFI CCNGG 2 cut(s) 475, 727
BpiI GAAGAC 2 cut(s) 301, 355
BpmI CTGGAG 1 cut(s) 124
BpuMI CCSGG 1 cut(s) 727
BsaHI GRCGYC 2 cut(s) 324, 609
BsaJI CCNNGG 5 cut(s) 163, 391, 429, 553, 629
BsaWI WCCGGW 1 cut(s) 218
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bsc4I CCNNNNNNNGG 5 cut(s) 218, 430, 474, 589, 705
Bse118I RCCGGY 1 cut(s) 731
Bse3DI GCAATG 1 cut(s) 84
BseBI CCWGG 1 cut(s) 475
BseDI CCNNGG 5 cut(s) 163, 391, 429, 553, 629
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 5 cut(s) 218, 430, 474, 589, 705
BseMI GCAATG 1 cut(s) 84
BseRI GAGGAG 3 cut(s) 173, 284, 513
BseXI GCAGC 2 cut(s) 131, 509
BseYI CCCAGC 1 cut(s) 317
Bsh1236I CGCG 1 cut(s) 631
BshFI GGCC 2 cut(s) 339, 668
BshNI GGYRCC 2 cut(s) 167, 608
BsiSI CCGG 3 cut(s) 219, 727, 732
BslI CCNNNNNNNGG 5 cut(s) 218, 430, 474, 589, 705
BsmAI GTCTC 1 cut(s) 525
BsmI GAATGC 1 cut(s) 398
BsnI GGCC 2 cut(s) 339, 668
Bsp1286I GDGCHC 2 cut(s) 119, 727
Bsp143I GATC 4 cut(s) 123, 289, 420, 511
BspACI CCGC 9 cut(s) 174, 213, 228, 376, 606, 612, 629, 631, 669
BspANI GGCC 2 cut(s) 339, 668
BspFNI CGCG 1 cut(s) 631
BspLI GGNNCC 4 cut(s) 116, 169, 567, 610
BspMI ACCTGC 1 cut(s) 380
BspPI GGATC 2 cut(s) 118, 415
BspQI GCTCTTC 1 cut(s) 362
BspT107I GGYRCC 2 cut(s) 167, 608
BsrDI GCAATG 1 cut(s) 84
BsrFI RCCGGY 1 cut(s) 731
BssAI RCCGGY 1 cut(s) 731
BssECI CCNNGG 5 cut(s) 163, 391, 429, 553, 629
BssMI GATC 4 cut(s) 123, 289, 420, 511
BssNI GRCGYC 2 cut(s) 324, 609
Bst2UI CCWGG 1 cut(s) 475
Bst6I CTCTTC 2 cut(s) 335, 362
BstACI GRCGYC 2 cut(s) 324, 609
BstC8I GCNNGC 1 cut(s) 465
BstDSI CCRYGG 4 cut(s) 163, 391, 553, 629
BstEII GGTNACC 1 cut(s) 433
BstENI CCTNNNNNAGG 1 cut(s) 428
BstF5I GGATG 1 cut(s) 198
BstFNI CGCG 1 cut(s) 631
BstH2I RGCGCY 1 cut(s) 612
BstHHI GCGC 2 cut(s) 398, 611
BstKTI GATC 4 cut(s) 126, 292, 423, 514
BstMAI GTCTC 1 cut(s) 525
BstMBI GATC 4 cut(s) 123, 289, 420, 511
BstNI CCWGG 1 cut(s) 475
BstPI GGTNACC 1 cut(s) 433
BstSCI CCNGG 2 cut(s) 473, 725
BstSFI CTRYAG 1 cut(s) 265
BstUI CGCG 1 cut(s) 631
BstV1I GCAGC 2 cut(s) 131, 509
BstV2I GAAGAC 2 cut(s) 301, 355
BstX2I RGATCY 1 cut(s) 123
BstYI RGATCY 1 cut(s) 123
BsuRI GGCC 2 cut(s) 339, 668
BtgI CCRYGG 4 cut(s) 163, 391, 553, 629
BtsCI GGATG 1 cut(s) 198
BveI ACCTGC 1 cut(s) 380
Cac8I GCNNGC 1 cut(s) 465
CaiI CAGNNNCTG 1 cut(s) 444
CfoI GCGC 2 cut(s) 398, 611
Cfr10I RCCGGY 1 cut(s) 731
Cfr13I GGNCC 2 cut(s) 566, 729
Cfr42I CCGCGG 1 cut(s) 632
CpoI CGGWCCG 1 cut(s) 729
CseI GACGC 2 cut(s) 259, 469
Csp6I GTAC 2 cut(s) 179, 580
CspI CGGWCCG 1 cut(s) 729
CviAII CATG 2 cut(s) 42, 64
CviJI RGCY 9 cut(s) 117, 137, 144, 339, 441, 500, 558, 668, 725
CviKI_1 RGCY 9 cut(s) 117, 137, 144, 339, 441, 500, 558, 668, 725
CviQI GTAC 2 cut(s) 179, 580
DinI GGCGCC 1 cut(s) 610
DpnI GATC 4 cut(s) 125, 291, 422, 513
DpnII GATC 4 cut(s) 123, 289, 420, 511
DrdI GACNNNNNNGTC 1 cut(s) 269
DseDI GACNNNNNNGTC 1 cut(s) 269
EaeI YGGCCR 2 cut(s) 337, 666
Eam1104I CTCTTC 2 cut(s) 335, 362
EarI CTCTTC 2 cut(s) 335, 362
Eco24I GRGCYC 2 cut(s) 119, 727
Eco47I GGWCC 2 cut(s) 566, 729
Eco91I GGTNACC 1 cut(s) 433
EcoNI CCTNNNNNAGG 1 cut(s) 428
EcoO65I GGTNACC 1 cut(s) 433
EcoRII CCWGG 1 cut(s) 473
EcoT38I GRGCYC 2 cut(s) 119, 727
EgeI GGCGCC 1 cut(s) 610
EheI GGCGCC 1 cut(s) 610
FaeI CATG 2 cut(s) 45, 67
FaiI YATR 3 cut(s) 43, 65, 510
FatI CATG 2 cut(s) 41, 63
FauI CCCGC 2 cut(s) 181, 220
Fnu4HI GCNGC 7 cut(s) 145, 228, 376, 498, 607, 612, 669
FokI GGATG 1 cut(s) 185
FriOI GRGCYC 2 cut(s) 119, 727
Fsp4HI GCNGC 7 cut(s) 145, 228, 376, 498, 607, 612, 669
FspBI CTAG 1 cut(s) 753
GlaI GCGC 2 cut(s) 397, 610
GluI GCNGC 7 cut(s) 145, 228, 376, 498, 607, 612, 669
GsaI CCCAGC 1 cut(s) 321
GsuI CTGGAG 1 cut(s) 124
HaeII RGCGCY 1 cut(s) 612
HaeIII GGCC 2 cut(s) 339, 668
HapII CCGG 3 cut(s) 219, 727, 732
HgaI GACGC 2 cut(s) 259, 469
HhaI GCGC 2 cut(s) 398, 611
Hin1I GRCGYC 2 cut(s) 324, 609
Hin1II CATG 2 cut(s) 45, 67
Hin6I GCGC 2 cut(s) 396, 609
HinP1I GCGC 2 cut(s) 396, 609
HinfI GANTC 2 cut(s) 222, 625
HpaII CCGG 3 cut(s) 219, 727, 732
HphI GGTGA 3 cut(s) 277, 361, 427
Hpy166II GTNNAC 1 cut(s) 580
Hpy188I TCNGA 2 cut(s) 128, 208
Hpy188III TCNNGA 3 cut(s) 247, 418, 457
Hpy8I GTNNAC 1 cut(s) 580
Hpy99I CGWCG 4 cut(s) 275, 326, 386, 620
HpyAV CCTTC 1 cut(s) 47
HpyCH4IV ACGT 3 cut(s) 324, 381, 453
HpyCH4V TGCA 1 cut(s) 497
HpySE526I ACGT 3 cut(s) 324, 381, 453
Hsp92I GRCGYC 2 cut(s) 324, 609
Hsp92II CATG 2 cut(s) 45, 67
HspAI GCGC 2 cut(s) 396, 609
KasI GGCGCC 1 cut(s) 608
KspI CCGCGG 1 cut(s) 632
Kzo9I GATC 4 cut(s) 123, 289, 420, 511
LguI GCTCTTC 1 cut(s) 362
LmnI GCTCC 1 cut(s) 114
Lsp1109I GCAGC 2 cut(s) 131, 509
MaeI CTAG 1 cut(s) 753
MaeII ACGT 3 cut(s) 324, 381, 453
MaeIII GTNAC 1 cut(s) 433
MalI GATC 4 cut(s) 125, 291, 422, 513
MboI GATC 4 cut(s) 123, 289, 420, 511
MboII GAAGA 7 cut(s) 46, 49, 301, 304, 322, 349, 355
MflI RGATCY 1 cut(s) 123
MhlI GDGCHC 2 cut(s) 119, 727
MluCI AATT 3 cut(s) 5, 713, 737
Mly113I GGCGCC 1 cut(s) 609
MmeI TCCRAC 2 cut(s) 151, 231
MspA1I CMGCKG 3 cut(s) 144, 606, 631
MspI CCGG 3 cut(s) 219, 727, 732
MspR9I CCNGG 2 cut(s) 475, 727
Mva1269I GAATGC 1 cut(s) 398
MvaI CCWGG 1 cut(s) 475
MvnI CGCG 1 cut(s) 631
NarI GGCGCC 1 cut(s) 609
NciI CCSGG 1 cut(s) 727
NdeII GATC 4 cut(s) 123, 289, 420, 511
NlaIII CATG 2 cut(s) 45, 67
NlaIV GGNNCC 4 cut(s) 116, 169, 567, 610
NmeAIII GCCGAG 1 cut(s) 454
NmuCI GTSAC 1 cut(s) 433
PaqCI CACCTGC 1 cut(s) 380
PciSI GCTCTTC 1 cut(s) 362
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 1 cut(s) 398
PfeI GAWTC 2 cut(s) 222, 625
PflMI CCANNNNNTGG 1 cut(s) 705
PkrI GCNGC 7 cut(s) 146, 229, 377, 499, 608, 613, 670
PluTI GGCGCC 1 cut(s) 612
Psp6I CCWGG 1 cut(s) 473
PspEI GGTNACC 1 cut(s) 433
PspFI CCCAGC 1 cut(s) 317
PspGI CCWGG 1 cut(s) 473
PspN4I GGNNCC 4 cut(s) 116, 169, 567, 610
PspPI GGNCC 2 cut(s) 566, 729
PstNI CAGNNNCTG 1 cut(s) 444
PsuI RGATCY 1 cut(s) 123
PvuII CAGCTG 1 cut(s) 144
RsaI GTAC 2 cut(s) 180, 581
RsaNI GTAC 2 cut(s) 179, 580
Rsr2I CGGWCCG 1 cut(s) 729
RsrII CGGWCCG 1 cut(s) 729
SacII CCGCGG 1 cut(s) 632
SapI GCTCTTC 1 cut(s) 362
SatI GCNGC 7 cut(s) 145, 228, 376, 498, 607, 612, 669
Sau3AI GATC 4 cut(s) 123, 289, 420, 511
Sau96I GGNCC 2 cut(s) 566, 729
ScrFI CCNGG 2 cut(s) 475, 727
SduI GDGCHC 2 cut(s) 119, 727
SfcI CTRYAG 1 cut(s) 265
SfoI GGCGCC 1 cut(s) 610
Sfr303I CCGCGG 1 cut(s) 632
SgrBI CCGCGG 1 cut(s) 632
SinI GGWCC 2 cut(s) 566, 729
Sse9I AATT 3 cut(s) 5, 713, 737
SsiI CCGC 9 cut(s) 174, 213, 228, 376, 606, 612, 629, 631, 669
SspDI GGCGCC 1 cut(s) 608
SspMI CTAG 1 cut(s) 753
StyD4I CCNGG 2 cut(s) 473, 725
TaiI ACGT 3 cut(s) 327, 384, 456
TaqI TCGA 2 cut(s) 261, 545
TasI AATT 3 cut(s) 5, 713, 737
TauI GCSGC 5 cut(s) 230, 378, 609, 614, 671
TfiI GAWTC 2 cut(s) 222, 625
TseFI GTSAC 1 cut(s) 433
TseI GCWGC 2 cut(s) 144, 497
Tsp45I GTSAC 1 cut(s) 433
TspDTI ATGAA 2 cut(s) 85, 322
TspGWI ACGGA 4 cut(s) 244, 542, 558, 633
Van91I CCANNNNNTGG 1 cut(s) 705
VpaK11BI GGWCC 2 cut(s) 566, 729
XagI CCTNNNNNAGG 1 cut(s) 428
XcmI CCANNNNNNNNNTGG 1 cut(s) 706
XspI CTAG 1 cut(s) 753
ZraI GACGTC 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.