RLG00000022277

TRAM, LAG1 and CLN8 homology domains.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004398
Physical Location & Seq
Reverse (-)
82158248 .. 82159901
1654 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022277

Sequence Viewer

Length: 909 bp
ATGGGAACGCAGCAAAGCCCAATTCTTTCTTCCCTCCCACCCCTCCCAATCCTCTTCTCCTTCTTCTTCACCGTCTACCTCATCGCCTACTTCCTCGTCTTCCGCTCGTGGAGCCCCAAGATCCGACCCGAAGCCTCCAGCTGCCTCATCTCCCTCGCCCACGGCACCCCCGCCGTCTTCTTCTCCTCCTTCGCCATTGTCTCCGATCCCTCCACCGGCTTCGCCGCCCCCAACACGCGCCTCCAGAACTCCGTTCTCGATTTCAGCGTCGCCTATTTCCTCATGGATCTTCTCCACTACCTCCTCTTCTTCCCCGGCGACGTCCTCTTCATCGGCCACCACCTCGCCACGCTCTTCGTCTTCCTCACCTGCCGCTACGTCGCGTCCCGCGGCGCGTTTGCTGTCCTCACGCTCCTTATCCTCGCCGAGGTCACCAGCCTCTGCCAGAACGTCTGGACGCTCGCCAACGCGCGCCGGAGGGACGTGGAGTTCGCCGCCAAGGTGTATGATTTTCTGTCTCCTCCGTTTTATGTTCTCTATTCGATTGTGAGGGGCTTTGTTGGCCCTTATTTCGTGTATAGAATGTGTGCTTTCTATGTTAGCGGCGCCGCCGAGGGCTTGATTCCCCGGTGGGTTTGGGTTTCGTGGATTGTGGTGATTAGTGTAAATGATGACTACAAGCAATGCCAGTCGAATGGTTTAATCATGATCACAGAACAGATTCAACGCAGTTTTTTGAATGCATACGTTTTCAGGCGATATGTCAATCTCAAAGATGATATTTTCTCCCCTTTCGTTATTGTGATTGAACCATCGACAATGCTTTCTTTGTTAGCAAACCAAATCTTCTTCATGTATGATGTGCATTCCTCTGTAACATTGTTCATCAAGTATAAAGCATACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573 TRAM_LAG1_CLN8

Protein Analysis

303

Amino Acids

34.43

Weight (kDa)

8.53

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 46 - 220 5.7e-17 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 377
AatII GACGTC 1 cut(s) 324
Acc36I ACCTGC 1 cut(s) 377
AccB1I GGYRCC 2 cut(s) 164, 605
AccBSI CCGCTC 1 cut(s) 105
AccI GTMKAC 1 cut(s) 75
AccII CGCG 6 cut(s) 238, 383, 390, 395, 470, 472
AciI CCGC 9 cut(s) 103, 171, 225, 373, 388, 390, 495, 603, 609
AclWI GGATC 3 cut(s) 115, 200, 294
AcoI YGGCCR 1 cut(s) 334
AcyI GRCGYC 2 cut(s) 321, 606
AfiI CCNNNNNNNGG 2 cut(s) 215, 427
AflIII ACRYGT 1 cut(s) 903
AgsI TTSAA 3 cut(s) 725, 739, 809
AjiI CACGTC 1 cut(s) 484
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AloI GAACNNNNNNTCC 2 cut(s) 473, 505
AluBI AGCT 1 cut(s) 141
AluI AGCT 1 cut(s) 141
Alw26I GTCTC 2 cut(s) 205, 522
AlwI GGATC 3 cut(s) 115, 200, 294
AlwNI CAGNNNCTG 1 cut(s) 441
AoxI GGCC 2 cut(s) 334, 562
ApeKI GCWGC 2 cut(s) 10, 141
ArsI GACNNNNNNTTYG 2 cut(s) 473, 505
Asp700I GAANNNNTTC 1 cut(s) 720
AspLEI GCGC 5 cut(s) 240, 395, 472, 474, 608
AspS9I GGNCC 1 cut(s) 563
AsuC2I CCSGG 2 cut(s) 315, 628
AsuHPI GGTGA 4 cut(s) 61, 358, 424, 667
BanI GGYRCC 2 cut(s) 164, 605
BanII GRGCYC 1 cut(s) 116
BauI CACGAG 1 cut(s) 106
BbsI GAAGAC 3 cut(s) 91, 169, 352
BbvI GCAGC 2 cut(s) 22, 128
BccI CCATC 1 cut(s) 820
BceAI ACGGC 2 cut(s) 158, 178
BclI TGATCA 1 cut(s) 708
BcnI CCSGG 2 cut(s) 315, 628
BcoDI GTCTC 2 cut(s) 205, 522
BfoI RGCGCY 1 cut(s) 609
BfuAI ACCTGC 1 cut(s) 377
BisI GCNGC 8 cut(s) 11, 142, 225, 373, 391, 495, 604, 609
BlsI GCNGC 8 cut(s) 12, 143, 226, 374, 392, 496, 605, 610
Bme1390I CCNGG 2 cut(s) 315, 628
BmgBI CACGTC 1 cut(s) 484
BmgT120I GGNCC 1 cut(s) 563
BmiI GGNNCC 3 cut(s) 113, 166, 607
BmrFI CCNGG 2 cut(s) 315, 628
BpiI GAAGAC 3 cut(s) 91, 169, 352
BpmI CTGGAG 2 cut(s) 121, 227
BpuMI CCSGG 2 cut(s) 315, 628
BsaHI GRCGYC 2 cut(s) 321, 606
BsaJI CCNNGG 7 cut(s) 160, 313, 388, 426, 498, 612, 626
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 427
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 1 cut(s) 688
Bse3DI GCAATG 1 cut(s) 689
BseDI CCNNGG 7 cut(s) 160, 313, 388, 426, 498, 612, 626
BseLI CCNNNNNNNGG 2 cut(s) 215, 427
BseMI GCAATG 1 cut(s) 689
BseNI ACTGG 1 cut(s) 688
BsePI GCGCGC 1 cut(s) 470
BseRI GAGGAG 3 cut(s) 175, 293, 510
BseXI GCAGC 2 cut(s) 22, 128
Bsh1236I CGCG 6 cut(s) 238, 383, 390, 395, 470, 472
BshFI GGCC 2 cut(s) 336, 564
BshNI GGYRCC 2 cut(s) 164, 605
BsiSI CCGG 4 cut(s) 216, 315, 475, 628
BslFI GGGAC 2 cut(s) 370, 494
BslI CCNNNNNNNGG 2 cut(s) 215, 427
BsmAI GTCTC 2 cut(s) 205, 522
BsmFI GGGAC 2 cut(s) 370, 494
BsmI GAATGC 2 cut(s) 745, 865
BsnI GGCC 2 cut(s) 336, 564
Bsp1286I GDGCHC 1 cut(s) 116
Bsp143I GATC 4 cut(s) 120, 205, 286, 708
BspACI CCGC 9 cut(s) 103, 171, 225, 373, 388, 390, 495, 603, 609
BspANI GGCC 2 cut(s) 336, 564
BspFNI CGCG 6 cut(s) 238, 383, 390, 395, 470, 472
BspHI TCATGA 1 cut(s) 705
BspLI GGNNCC 3 cut(s) 113, 166, 607
BspMI ACCTGC 1 cut(s) 377
BspPI GGATC 3 cut(s) 115, 200, 294
BspQI GCTCTTC 1 cut(s) 359
BspT107I GGYRCC 2 cut(s) 164, 605
BsrBI CCGCTC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 689
BsrFI RCCGGY 1 cut(s) 215
BsrI ACTGG 1 cut(s) 688
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 7 cut(s) 160, 313, 388, 426, 498, 612, 626
BssHII GCGCGC 1 cut(s) 470
BssMI GATC 4 cut(s) 120, 205, 286, 708
BssNI GRCGYC 2 cut(s) 321, 606
BssSI CACGAG 1 cut(s) 106
BssT1I CCWWGG 1 cut(s) 498
Bst2BI CACGAG 1 cut(s) 106
Bst4CI ACNGT 1 cut(s) 73
Bst6I CTCTTC 4 cut(s) 59, 311, 332, 359
BstACI GRCGYC 2 cut(s) 321, 606
BstC8I GCNNGC 2 cut(s) 462, 472
BstDSI CCRYGG 2 cut(s) 160, 388
BstEII GGTNACC 1 cut(s) 430
BstENI CCTNNNNNAGG 1 cut(s) 425
BstFNI CGCG 6 cut(s) 238, 383, 390, 395, 470, 472
BstH2I RGCGCY 1 cut(s) 609
BstHHI GCGC 5 cut(s) 240, 395, 472, 474, 608
BstKTI GATC 4 cut(s) 123, 208, 289, 711
BstMAI GTCTC 2 cut(s) 205, 522
BstMBI GATC 4 cut(s) 120, 205, 286, 708
BstMWI GCNNNNNNNGC 2 cut(s) 111, 561
BstNSI RCATGY 1 cut(s) 907
BstPI GGTNACC 1 cut(s) 430
BstSCI CCNGG 2 cut(s) 313, 626
BstUI CGCG 6 cut(s) 238, 383, 390, 395, 470, 472
BstV1I GCAGC 2 cut(s) 22, 128
BstV2I GAAGAC 3 cut(s) 91, 169, 352
BstX2I RGATCY 2 cut(s) 120, 286
BstXI CCANNNNNNTGG 1 cut(s) 695
BstYI RGATCY 2 cut(s) 120, 286
BsuRI GGCC 2 cut(s) 336, 564
BtgI CCRYGG 2 cut(s) 160, 388
BtgZI GCGATG 1 cut(s) 67
BtrI CACGTC 1 cut(s) 484
BveI ACCTGC 1 cut(s) 377
Cac8I GCNNGC 2 cut(s) 462, 472
CaiI CAGNNNCTG 1 cut(s) 441
CciI TCATGA 1 cut(s) 705
CfoI GCGC 5 cut(s) 240, 395, 472, 474, 608
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 563
Cfr42I CCGCGG 1 cut(s) 391
CseI GACGC 3 cut(s) 256, 372, 466
CviAII CATG 4 cut(s) 283, 706, 853, 904
DinI GGCGCC 1 cut(s) 607
DpnI GATC 4 cut(s) 122, 207, 288, 710
DpnII GATC 4 cut(s) 120, 205, 286, 708
EaeI YGGCCR 1 cut(s) 334
Eam1104I CTCTTC 4 cut(s) 59, 311, 332, 359
EarI CTCTTC 4 cut(s) 59, 311, 332, 359
Eco130I CCWWGG 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 116
Eco91I GGTNACC 1 cut(s) 430
EcoNI CCTNNNNNAGG 1 cut(s) 425
EcoO65I GGTNACC 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 498
EcoT22I ATGCAT 1 cut(s) 745
EcoT38I GRGCYC 1 cut(s) 116
EgeI GGCGCC 1 cut(s) 607
EheI GGCGCC 1 cut(s) 607
ErhI CCWWGG 1 cut(s) 498
FaeI CATG 4 cut(s) 286, 709, 856, 907
FaqI GGGAC 2 cut(s) 370, 494
FatI CATG 4 cut(s) 282, 705, 852, 903
FauI CCCGC 2 cut(s) 178, 395
FbaI TGATCA 1 cut(s) 708
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 8 cut(s) 11, 142, 225, 373, 391, 495, 604, 609
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 8 cut(s) 11, 142, 225, 373, 391, 495, 604, 609
GlaI GCGC 5 cut(s) 239, 394, 471, 473, 607
GluI GCNGC 8 cut(s) 11, 142, 225, 373, 391, 495, 604, 609
GsuI CTGGAG 2 cut(s) 121, 227
HaeII RGCGCY 1 cut(s) 609
HaeIII GGCC 2 cut(s) 336, 564
HapII CCGG 4 cut(s) 216, 315, 475, 628
HgaI GACGC 3 cut(s) 256, 372, 466
HhaI GCGC 5 cut(s) 240, 395, 472, 474, 608
Hin1I GRCGYC 2 cut(s) 321, 606
Hin1II CATG 4 cut(s) 286, 709, 856, 907
Hin6I GCGC 5 cut(s) 238, 393, 470, 472, 606
HinP1I GCGC 5 cut(s) 238, 393, 470, 472, 606
HinfI GANTC 2 cut(s) 622, 721
HpaII CCGG 4 cut(s) 216, 315, 475, 628
HphI GGTGA 4 cut(s) 61, 358, 424, 667
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 2 cut(s) 125, 205
Hpy188III TCNNGA 4 cut(s) 244, 257, 454, 706
Hpy8I GTNNAC 1 cut(s) 76
Hpy99I CGWCG 3 cut(s) 272, 323, 383
HpyAV CCTTC 2 cut(s) 70, 199
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4IV ACGT 5 cut(s) 321, 378, 450, 483, 747
HpyCH4V TGCA 2 cut(s) 743, 865
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 561
HpySE526I ACGT 5 cut(s) 321, 378, 450, 483, 747
Hsp92I GRCGYC 2 cut(s) 321, 606
Hsp92II CATG 4 cut(s) 286, 709, 856, 907
HspAI GCGC 5 cut(s) 238, 393, 470, 472, 606
KasI GGCGCC 1 cut(s) 605
Ksp22I TGATCA 1 cut(s) 708
KspI CCGCGG 1 cut(s) 391
Kzo9I GATC 4 cut(s) 120, 205, 286, 708
LguI GCTCTTC 1 cut(s) 359
LmnI GCTCC 2 cut(s) 111, 417
Lsp1109I GCAGC 2 cut(s) 22, 128
MaeII ACGT 5 cut(s) 321, 378, 450, 483, 747
MaeIII GTNAC 2 cut(s) 430, 874
MalI GATC 4 cut(s) 122, 207, 288, 710
MbiI CCGCTC 1 cut(s) 105
MboI GATC 4 cut(s) 120, 205, 286, 708
MflI RGATCY 2 cut(s) 120, 286
MhlI GDGCHC 1 cut(s) 116
MluCI AATT 1 cut(s) 21
Mly113I GGCGCC 1 cut(s) 606
MmeI TCCRAC 1 cut(s) 148
Mph1103I ATGCAT 1 cut(s) 745
MroXI GAANNNNTTC 1 cut(s) 720
MseI TTAA 1 cut(s) 701
MslI CAYNNNNRTG 1 cut(s) 904
MspA1I CMGCKG 2 cut(s) 141, 390
MspI CCGG 4 cut(s) 216, 315, 475, 628
MspR9I CCNGG 2 cut(s) 315, 628
Mva1269I GAATGC 2 cut(s) 745, 865
MvnI CGCG 6 cut(s) 238, 383, 390, 395, 470, 472
MwoI GCNNNNNNNGC 2 cut(s) 111, 561
NarI GGCGCC 1 cut(s) 606
NciI CCSGG 2 cut(s) 315, 628
NdeII GATC 4 cut(s) 120, 205, 286, 708
NlaIII CATG 4 cut(s) 286, 709, 856, 907
NlaIV GGNNCC 3 cut(s) 113, 166, 607
NmeAIII GCCGAG 2 cut(s) 451, 637
NmuCI GTSAC 1 cut(s) 430
NsiI ATGCAT 1 cut(s) 745
NspI RCATGY 1 cut(s) 907
PagI TCATGA 1 cut(s) 705
PaqCI CACCTGC 1 cut(s) 377
PauI GCGCGC 1 cut(s) 470
PciI ACATGT 1 cut(s) 903
PciSI GCTCTTC 1 cut(s) 359
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PctI GAATGC 2 cut(s) 745, 865
PdmI GAANNNNTTC 1 cut(s) 720
PfeI GAWTC 2 cut(s) 622, 721
PkrI GCNGC 8 cut(s) 12, 143, 226, 374, 392, 496, 605, 610
PluTI GGCGCC 1 cut(s) 609
PscI ACATGT 1 cut(s) 903
PspEI GGTNACC 1 cut(s) 430
PspN4I GGNNCC 3 cut(s) 113, 166, 607
PspPI GGNCC 1 cut(s) 563
PstNI CAGNNNCTG 1 cut(s) 441
PsuI RGATCY 2 cut(s) 120, 286
PteI GCGCGC 1 cut(s) 470
PvuII CAGCTG 1 cut(s) 141
RseI CAYNNNNRTG 1 cut(s) 904
SacII CCGCGG 1 cut(s) 391
SapI GCTCTTC 1 cut(s) 359
SaqAI TTAA 1 cut(s) 701
SatI GCNGC 8 cut(s) 11, 142, 225, 373, 391, 495, 604, 609
Sau3AI GATC 4 cut(s) 120, 205, 286, 708
Sau96I GGNCC 1 cut(s) 563
ScrFI CCNGG 2 cut(s) 315, 628
SduI GDGCHC 1 cut(s) 116
SfoI GGCGCC 1 cut(s) 607
Sfr303I CCGCGG 1 cut(s) 391
SgrBI CCGCGG 1 cut(s) 391
SmiMI CAYNNNNRTG 1 cut(s) 904
Sse9I AATT 1 cut(s) 21
SsiI CCGC 9 cut(s) 103, 171, 225, 373, 388, 390, 495, 603, 609
SspDI GGCGCC 1 cut(s) 605
StyD4I CCNGG 2 cut(s) 313, 626
StyI CCWWGG 1 cut(s) 498
TaaI ACNGT 1 cut(s) 73
TaiI ACGT 5 cut(s) 324, 381, 453, 486, 750
TaqI TCGA 4 cut(s) 258, 542, 692, 815
TasI AATT 1 cut(s) 21
TauI GCSGC 6 cut(s) 227, 375, 393, 497, 606, 611
TfiI GAWTC 2 cut(s) 622, 721
Tru1I TTAA 1 cut(s) 701
Tru9I TTAA 1 cut(s) 701
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 2 cut(s) 10, 141
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 3 cut(s) 319, 841, 874
TspGWI ACGGA 2 cut(s) 241, 513
XagI CCTNNNNNAGG 1 cut(s) 425
XceI RCATGY 1 cut(s) 907
XmiI GTMKAC 1 cut(s) 75
XmnI GAANNNNTTC 1 cut(s) 720
ZraI GACGTC 1 cut(s) 322
Zsp2I ATGCAT 1 cut(s) 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.