Rh2AG659300

TRAM, LAG1 and CLN8 homology domains.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0004398
Physical Location & Seq
Reverse (-)
87538166 .. 87538639
474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG659300.1

Sequence Viewer

Length: 474 bp
ATGGATCTTCTCCACTACCTCCTCTTCTTCCCCGGCGACGTCCTCTTCATCGGCCACCACCTCGCCACGCTCTTCGTCTTCCTCACCTGCCGCTACGTCGCCTCCCGCGGCGCCTTCGCCGTCCTCACGCTCCTCATCCTCGCCGAGGTCACCAGCCTCTGCCAGAACGTCTGGACGCTCGCCAACGCGCGCCGGAGGGACGTGGAGTTCGCCGCCAAGGTGTACGATTTTCTGTCTCCTCCGTTTTATGTCCTCTATTCGATTGTGAGGGGCTTTGTTGGCCCTTGTTTCGTGTATAGGATGTGTGCTTTCTACGCTAGCGGCGCCGCCGAGGGCTTGATTCCCCGGTGGGTTTGGGTTTCGTGGATTGTGGTGGTAATGTCTGCTATTTCTGTTAGCATTATGTGGATTTCGAATCTTTGGGTGGAGTTGTATAGAGATAGGAGTGGCAAGTTGGAAAAGAAAGTTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573 TRAM_LAG1_CLN8

Protein Analysis

157

Amino Acids

17.97

Weight (kDa)

8.98

Isoelectric Point (pI)

31.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 3 - 141 9.2e-12 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 95
AatII GACGTC 1 cut(s) 42
Acc36I ACCTGC 1 cut(s) 95
AccB1I GGYRCC 2 cut(s) 110, 323
AccII CGCG 3 cut(s) 108, 188, 190
AciI CCGC 6 cut(s) 91, 106, 108, 213, 321, 327
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 52
AcyI GRCGYC 3 cut(s) 39, 111, 324
AfaI GTAC 1 cut(s) 224
AfiI CCNNNNNNNGG 1 cut(s) 145
AjiI CACGTC 1 cut(s) 202
AloI GAACNNNNNNTCC 2 cut(s) 191, 223
Alw26I GTCTC 1 cut(s) 240
AlwI GGATC 1 cut(s) 12
AlwNI CAGNNNCTG 1 cut(s) 159
AoxI GGCC 2 cut(s) 52, 280
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AspLEI GCGC 4 cut(s) 113, 190, 192, 326
AspS9I GGNCC 1 cut(s) 281
AsuC2I CCSGG 2 cut(s) 33, 346
AsuHPI GGTGA 2 cut(s) 76, 142
AsuII TTCGAA 1 cut(s) 413
AsuNHI GCTAGC 1 cut(s) 317
BanI GGYRCC 2 cut(s) 110, 323
BbsI GAAGAC 1 cut(s) 70
BceAI ACGGC 1 cut(s) 104
BcnI CCSGG 2 cut(s) 33, 346
BcoDI GTCTC 1 cut(s) 240
BfaI CTAG 1 cut(s) 318
BfoI RGCGCY 2 cut(s) 114, 327
BfuAI ACCTGC 1 cut(s) 95
BisI GCNGC 5 cut(s) 91, 109, 213, 322, 327
BlsI GCNGC 5 cut(s) 92, 110, 214, 323, 328
Bme1390I CCNGG 2 cut(s) 33, 346
BmgBI CACGTC 1 cut(s) 202
BmgT120I GGNCC 1 cut(s) 281
BmiI GGNNCC 2 cut(s) 112, 325
BmrFI CCNGG 2 cut(s) 33, 346
BmtI GCTAGC 1 cut(s) 321
BpiI GAAGAC 1 cut(s) 70
Bpu14I TTCGAA 1 cut(s) 413
BpuMI CCSGG 2 cut(s) 33, 346
BsaHI GRCGYC 3 cut(s) 39, 111, 324
BsaJI CCNNGG 6 cut(s) 31, 106, 144, 216, 330, 344
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 1 cut(s) 145
BseDI CCNNGG 6 cut(s) 31, 106, 144, 216, 330, 344
BseGI GGATG 2 cut(s) 135, 306
BseLI CCNNNNNNNGG 1 cut(s) 145
BsePI GCGCGC 1 cut(s) 188
BseRI GAGGAG 3 cut(s) 11, 122, 228
Bsh1236I CGCG 3 cut(s) 108, 188, 190
BshFI GGCC 2 cut(s) 54, 282
BshNI GGYRCC 2 cut(s) 110, 323
BsiSI CCGG 3 cut(s) 33, 193, 346
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 1 cut(s) 145
BsmAI GTCTC 1 cut(s) 240
BsmFI GGGAC 1 cut(s) 212
BsnI GGCC 2 cut(s) 54, 282
Bsp119I TTCGAA 1 cut(s) 413
Bsp143I GATC 1 cut(s) 4
BspACI CCGC 6 cut(s) 91, 106, 108, 213, 321, 327
BspANI GGCC 2 cut(s) 54, 282
BspFNI CGCG 3 cut(s) 108, 188, 190
BspLI GGNNCC 2 cut(s) 112, 325
BspMI ACCTGC 1 cut(s) 95
BspOI GCTAGC 1 cut(s) 321
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 77
BspT104I TTCGAA 1 cut(s) 413
BspT107I GGYRCC 2 cut(s) 110, 323
BssECI CCNNGG 6 cut(s) 31, 106, 144, 216, 330, 344
BssHII GCGCGC 1 cut(s) 188
BssMI GATC 1 cut(s) 4
BssNI GRCGYC 3 cut(s) 39, 111, 324
BssT1I CCWWGG 1 cut(s) 216
Bst6I CTCTTC 3 cut(s) 29, 50, 77
BstACI GRCGYC 3 cut(s) 39, 111, 324
BstBI TTCGAA 1 cut(s) 413
BstC8I GCNNGC 3 cut(s) 180, 190, 319
BstDSI CCRYGG 1 cut(s) 106
BstEII GGTNACC 1 cut(s) 148
BstENI CCTNNNNNAGG 1 cut(s) 143
BstF5I GGATG 2 cut(s) 135, 306
BstFNI CGCG 3 cut(s) 108, 188, 190
BstH2I RGCGCY 2 cut(s) 114, 327
BstHHI GCGC 4 cut(s) 113, 190, 192, 326
BstKTI GATC 1 cut(s) 7
BstMAI GTCTC 1 cut(s) 240
BstMBI GATC 1 cut(s) 4
BstMWI GCNNNNNNNGC 3 cut(s) 279, 314, 323
BstPI GGTNACC 1 cut(s) 148
BstSCI CCNGG 2 cut(s) 31, 344
BstUI CGCG 3 cut(s) 108, 188, 190
BstV2I GAAGAC 1 cut(s) 70
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 2 cut(s) 54, 282
BtgI CCRYGG 1 cut(s) 106
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 2 cut(s) 135, 306
BveI ACCTGC 1 cut(s) 95
Cac8I GCNNGC 3 cut(s) 180, 190, 319
CaiI CAGNNNCTG 1 cut(s) 159
CfoI GCGC 4 cut(s) 113, 190, 192, 326
Cfr13I GGNCC 1 cut(s) 281
Cfr42I CCGCGG 1 cut(s) 109
CseI GACGC 1 cut(s) 184
Csp6I GTAC 1 cut(s) 223
CviJI RGCY 5 cut(s) 54, 156, 273, 282, 336
CviKI_1 RGCY 5 cut(s) 54, 156, 273, 282, 336
CviQI GTAC 1 cut(s) 223
DinI GGCGCC 2 cut(s) 112, 325
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
EaeI YGGCCR 1 cut(s) 52
Eam1104I CTCTTC 3 cut(s) 29, 50, 77
EarI CTCTTC 3 cut(s) 29, 50, 77
Eco130I CCWWGG 1 cut(s) 216
Eco91I GGTNACC 1 cut(s) 148
EcoNI CCTNNNNNAGG 1 cut(s) 143
EcoO65I GGTNACC 1 cut(s) 148
EcoT14I CCWWGG 1 cut(s) 216
EgeI GGCGCC 2 cut(s) 112, 325
EheI GGCGCC 2 cut(s) 112, 325
ErhI CCWWGG 1 cut(s) 216
FaiI YATR 5 cut(s) 249, 297, 404, 435, 472
FaqI GGGAC 1 cut(s) 212
FauI CCCGC 1 cut(s) 113
Fnu4HI GCNGC 5 cut(s) 91, 109, 213, 322, 327
FokI GGATG 2 cut(s) 122, 313
Fsp4HI GCNGC 5 cut(s) 91, 109, 213, 322, 327
FspBI CTAG 1 cut(s) 318
GlaI GCGC 4 cut(s) 112, 189, 191, 325
GluI GCNGC 5 cut(s) 91, 109, 213, 322, 327
HaeII RGCGCY 2 cut(s) 114, 327
HaeIII GGCC 2 cut(s) 54, 282
HapII CCGG 3 cut(s) 33, 193, 346
HgaI GACGC 1 cut(s) 184
HhaI GCGC 4 cut(s) 113, 190, 192, 326
Hin1I GRCGYC 3 cut(s) 39, 111, 324
Hin6I GCGC 4 cut(s) 111, 188, 190, 324
HinP1I GCGC 4 cut(s) 111, 188, 190, 324
HinfI GANTC 2 cut(s) 340, 415
HpaII CCGG 3 cut(s) 33, 193, 346
HphI GGTGA 2 cut(s) 76, 142
Hpy166II GTNNAC 1 cut(s) 223
Hpy188III TCNNGA 1 cut(s) 172
Hpy8I GTNNAC 1 cut(s) 223
Hpy99I CGWCG 2 cut(s) 41, 101
HpyAV CCTTC 1 cut(s) 124
HpyCH4IV ACGT 4 cut(s) 39, 96, 168, 201
HpyF10VI GCNNNNNNNGC 3 cut(s) 279, 314, 323
HpySE526I ACGT 4 cut(s) 39, 96, 168, 201
Hsp92I GRCGYC 3 cut(s) 39, 111, 324
HspAI GCGC 4 cut(s) 111, 188, 190, 324
KasI GGCGCC 2 cut(s) 110, 323
KspI CCGCGG 1 cut(s) 109
Kzo9I GATC 1 cut(s) 4
LguI GCTCTTC 1 cut(s) 77
LmnI GCTCC 1 cut(s) 135
LpnPI CCDG 7 cut(s) 46, 100, 157, 166, 176, 206, 359
MaeI CTAG 1 cut(s) 318
MaeII ACGT 4 cut(s) 39, 96, 168, 201
MaeIII GTNAC 2 cut(s) 148, 466
MalI GATC 1 cut(s) 6
MboI GATC 1 cut(s) 4
MboII GAAGA 5 cut(s) 16, 19, 37, 64, 70
MflI RGATCY 1 cut(s) 4
Mly113I GGCGCC 2 cut(s) 111, 324
MmeI TCCRAC 1 cut(s) 435
MspA1I CMGCKG 1 cut(s) 108
MspI CCGG 3 cut(s) 33, 193, 346
MspR9I CCNGG 2 cut(s) 33, 346
MvnI CGCG 3 cut(s) 108, 188, 190
MwoI GCNNNNNNNGC 3 cut(s) 279, 314, 323
NarI GGCGCC 2 cut(s) 111, 324
NciI CCSGG 2 cut(s) 33, 346
NdeII GATC 1 cut(s) 4
NheI GCTAGC 1 cut(s) 317
NlaIV GGNNCC 2 cut(s) 112, 325
NmeAIII GCCGAG 2 cut(s) 169, 355
NmuCI GTSAC 1 cut(s) 148
NspV TTCGAA 1 cut(s) 413
PaqCI CACCTGC 1 cut(s) 95
PauI GCGCGC 1 cut(s) 188
PciSI GCTCTTC 1 cut(s) 77
PfeI GAWTC 2 cut(s) 340, 415
PkrI GCNGC 5 cut(s) 92, 110, 214, 323, 328
PluTI GGCGCC 2 cut(s) 114, 327
PspEI GGTNACC 1 cut(s) 148
PspN4I GGNNCC 2 cut(s) 112, 325
PspPI GGNCC 1 cut(s) 281
PstNI CAGNNNCTG 1 cut(s) 159
PsuI RGATCY 1 cut(s) 4
PteI GCGCGC 1 cut(s) 188
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SacII CCGCGG 1 cut(s) 109
SapI GCTCTTC 1 cut(s) 77
SatI GCNGC 5 cut(s) 91, 109, 213, 322, 327
Sau3AI GATC 1 cut(s) 4
Sau96I GGNCC 1 cut(s) 281
ScrFI CCNGG 2 cut(s) 33, 346
SetI ASST 9 cut(s) 21, 42, 63, 89, 99, 150, 171, 204, 222
SfoI GGCGCC 2 cut(s) 112, 325
Sfr303I CCGCGG 1 cut(s) 109
SfuI TTCGAA 1 cut(s) 413
SgrBI CCGCGG 1 cut(s) 109
SsiI CCGC 6 cut(s) 91, 106, 108, 213, 321, 327
SspDI GGCGCC 2 cut(s) 110, 323
SspMI CTAG 1 cut(s) 318
StyD4I CCNGG 2 cut(s) 31, 344
StyI CCWWGG 1 cut(s) 216
TaiI ACGT 4 cut(s) 42, 99, 171, 204
TaqI TCGA 2 cut(s) 260, 413
TauI GCSGC 5 cut(s) 93, 111, 215, 324, 329
TfiI GAWTC 2 cut(s) 340, 415
TseFI GTSAC 1 cut(s) 148
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 1 cut(s) 37
TspGWI ACGGA 1 cut(s) 231
XagI CCTNNNNNAGG 1 cut(s) 143
XspI CTAG 1 cut(s) 318
ZraI GACGTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.