Rh4BG312600

TRAM, LAG1 and CLN8 homology domains.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004398
Physical Location & Seq
Forward (+)
48052807 .. 48057638
4832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG312600.1

Sequence Viewer

Length: 738 bp
ATGGAGACCCCAGTTCTTTTCACCCCAACACTCCCCACATTCTTCACCATGTTCTTGCTCATATATCTCTTCGCTAGATTCGTGCTTTTTCGAAGCTGGGGTCCCAAACATAGGCCAGAAGCTTCCAGCTGTGTAACATCTCTAGCTCATAGCACTCCTGCAGTTTTCATGGCGATCCATGCACTAATTCATAGCCAGACAACTTCCATTTTCGCCTCCCAAAACACAGCTTTCGAAAACACTGTGCTTGAATACAGCATAGCTTACTTCTTGGTTGACCTTCTTCACTACTTGCTCTTCTTCCCTTCTGAAGTCCTCTTCATTCTTCACCACTTGGCCACACTGTACGTGTTTTTGACTTGCCGCTATGTTGTTCATCATGGGGCTTATGCCATCATTGTGCTTCTTTTTCTTGCTGAGATCACCAGCGGTTGCCAGAACGTTTGGACACTGGCGAGTTTTCGAAGGGATGATTCGGCTTCTGCTGCAAAACTGTATGACTTCTTGTCTCCTCGGTTTTATGCTTTTTACACTGTTTTTAGAGCGGTTCTGGGGCCTCTGTTTATGTTTAAGATGGGATTGTTCTATGTGAGTGGGGCGGCTGGAGAAGAAGTTCCGACATGGGCTTGGGTTTCTTGGATGGTTGTGATTGTAATAGCTATCTGTGTTAGCATATTGTGGGTTTTGAGTCATTGGAAAGATTGGTACAGAGACAAAGTTCAGAAGAAAGAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

245

Amino Acids

28.26

Weight (kDa)

8.32

Isoelectric Point (pI)

33.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 42 - 230 1.9e-16 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 545
AciI CCGC 4 cut(s) 364, 429, 545, 599
AclI AACGTT 1 cut(s) 441
AclWI GGATC 1 cut(s) 169
AcoI YGGCCR 1 cut(s) 336
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 2 cut(s) 347, 707
AfiI CCNNNNNNNGG 1 cut(s) 111
AflIII ACRYGT 1 cut(s) 348
AgsI TTSAA 1 cut(s) 251
AhdI GACNNNNNGTC 1 cut(s) 505
AloI GAACNNNNNNTCC 2 cut(s) 597, 629
AluBI AGCT 7 cut(s) 96, 122, 129, 146, 230, 263, 659
AluI AGCT 7 cut(s) 96, 122, 129, 146, 230, 263, 659
Alw26I GTCTC 2 cut(s) 513, 705
AlwI GGATC 1 cut(s) 169
AoxI GGCC 3 cut(s) 113, 336, 554
ApeKI GCWGC 1 cut(s) 485
ArsI GACNNNNNNTTYG 2 cut(s) 85, 117
Asp700I GAANNNNTTC 1 cut(s) 612
AspS9I GGNCC 2 cut(s) 101, 554
AsuHPI GGTGA 4 cut(s) 13, 37, 320, 415
AsuII TTCGAA 3 cut(s) 91, 234, 463
AvaII GGWCC 1 cut(s) 101
BalI TGGCCA 1 cut(s) 338
BbvI GCAGC 1 cut(s) 472
BccI CCATC 3 cut(s) 401, 568, 634
BcoDI GTCTC 2 cut(s) 513, 705
BfaI CTAG 2 cut(s) 75, 143
BfmI CTRYAG 1 cut(s) 159
BisI GCNGC 3 cut(s) 364, 486, 600
BlsI GCNGC 3 cut(s) 365, 487, 601
Bme18I GGWCC 1 cut(s) 101
BmeRI GACNNNNNGTC 1 cut(s) 505
BmgT120I GGNCC 2 cut(s) 101, 554
BmiI GGNNCC 3 cut(s) 102, 103, 555
BmrI ACTGGG 1 cut(s) 5
BmuI ACTGGG 1 cut(s) 5
BpmI CTGGAG 1 cut(s) 624
Bpu14I TTCGAA 3 cut(s) 91, 234, 463
BsaAI YACGTR 1 cut(s) 349
BsaJI CCNNGG 1 cut(s) 512
BsaXI ACNNNNNCTCC 2 cut(s) 597, 627
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse1I ACTGG 2 cut(s) 11, 456
BseDI CCNNGG 1 cut(s) 512
BseGI GGATG 2 cut(s) 475, 645
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 2 cut(s) 11, 456
BseRI GAGGAG 1 cut(s) 501
BseXI GCAGC 1 cut(s) 472
BseYI CCCAGC 1 cut(s) 96
BshFI GGCC 3 cut(s) 115, 338, 556
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 2 cut(s) 513, 705
BsmFI GGGAC 1 cut(s) 87
BsnI GGCC 3 cut(s) 115, 338, 556
Bsp119I TTCGAA 3 cut(s) 91, 234, 463
Bsp143I GATC 2 cut(s) 174, 420
BspACI CCGC 4 cut(s) 364, 429, 545, 599
BspANI GGCC 3 cut(s) 115, 338, 556
BspCNI CTCAG 1 cut(s) 409
BspLI GGNNCC 3 cut(s) 102, 103, 555
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 1 cut(s) 169
BspQI GCTCTTC 1 cut(s) 302
BspT104I TTCGAA 3 cut(s) 91, 234, 463
BsrBI CCGCTC 1 cut(s) 545
BsrI ACTGG 2 cut(s) 11, 456
BssECI CCNNGG 1 cut(s) 512
BssMI GATC 2 cut(s) 174, 420
Bst4CI ACNGT 4 cut(s) 244, 345, 495, 535
Bst6I CTCTTC 3 cut(s) 74, 302, 323
BstBAI YACGTR 1 cut(s) 349
BstBI TTCGAA 3 cut(s) 91, 234, 463
BstDEI CTNAG 1 cut(s) 417
BstF5I GGATG 2 cut(s) 475, 645
BstKTI GATC 2 cut(s) 177, 423
BstMAI GTCTC 2 cut(s) 513, 705
BstMBI GATC 2 cut(s) 174, 420
BstMWI GCNNNNNNNGC 2 cut(s) 179, 485
BstSFI CTRYAG 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 472
BsuRI GGCC 3 cut(s) 115, 338, 556
BtsCI GGATG 2 cut(s) 475, 645
BtsIMutI CAGTG 4 cut(s) 240, 341, 449, 531
Cfr13I GGNCC 2 cut(s) 101, 554
Csp6I GTAC 2 cut(s) 346, 706
CviAII CATG 5 cut(s) 49, 169, 179, 380, 621
CviQI GTAC 2 cut(s) 346, 706
DdeI CTNAG 1 cut(s) 417
DpnI GATC 2 cut(s) 176, 422
DpnII GATC 2 cut(s) 174, 420
DriI GACNNNNNGTC 1 cut(s) 505
EaeI YGGCCR 1 cut(s) 336
Eam1104I CTCTTC 3 cut(s) 74, 302, 323
Eam1105I GACNNNNNGTC 1 cut(s) 505
EarI CTCTTC 3 cut(s) 74, 302, 323
Eco47I GGWCC 1 cut(s) 101
Eco57I CTGAAG 1 cut(s) 330
EcoO109I RGGNCCY 2 cut(s) 101, 554
FaeI CATG 5 cut(s) 52, 172, 182, 383, 624
FaqI GGGAC 1 cut(s) 87
FatI CATG 5 cut(s) 48, 168, 178, 379, 620
Fnu4HI GCNGC 3 cut(s) 364, 486, 600
FokI GGATG 2 cut(s) 482, 652
Fsp4HI GCNGC 3 cut(s) 364, 486, 600
FspBI CTAG 2 cut(s) 75, 143
GluI GCNGC 3 cut(s) 364, 486, 600
GsaI CCCAGC 1 cut(s) 100
GsuI CTGGAG 1 cut(s) 624
HaeIII GGCC 3 cut(s) 115, 338, 556
Hin1II CATG 5 cut(s) 52, 172, 182, 383, 624
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 120
HinfI GANTC 3 cut(s) 78, 473, 688
HphI GGTGA 4 cut(s) 13, 37, 320, 415
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 3 cut(s) 310, 618, 723
Hpy8I GTNNAC 1 cut(s) 277
HpyAV CCTTC 3 cut(s) 290, 315, 459
HpyCH4III ACNGT 4 cut(s) 244, 345, 495, 535
HpyCH4IV ACGT 2 cut(s) 348, 441
HpyCH4V TGCA 3 cut(s) 161, 182, 488
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 485
HpyF3I CTNAG 1 cut(s) 417
HpySE526I ACGT 2 cut(s) 348, 441
Hsp92II CATG 5 cut(s) 52, 172, 182, 383, 624
KflI GGGWCCC 1 cut(s) 101
Kzo9I GATC 2 cut(s) 174, 420
LguI GCTCTTC 1 cut(s) 302
Lsp1109I GCAGC 1 cut(s) 472
MaeI CTAG 2 cut(s) 75, 143
MaeII ACGT 2 cut(s) 348, 441
MaeIII GTNAC 1 cut(s) 133
MalI GATC 2 cut(s) 176, 422
MbiI CCGCTC 1 cut(s) 545
MboI GATC 2 cut(s) 174, 420
MboII GAAGA 9 cut(s) 34, 61, 275, 289, 292, 310, 317, 620, 736
MlsI TGGCCA 1 cut(s) 338
MluCI AATT 1 cut(s) 186
MluNI TGGCCA 1 cut(s) 338
MlyI GAGTC 1 cut(s) 697
MmeI TCCRAC 1 cut(s) 641
MnlI CCTC 5 cut(s) 226, 326, 522, 567, 726
Mox20I TGGCCA 1 cut(s) 338
MroXI GAANNNNTTC 1 cut(s) 612
MscI TGGCCA 1 cut(s) 338
MseI TTAA 1 cut(s) 570
MslI CAYNNNNRTG 1 cut(s) 398
Msp20I TGGCCA 1 cut(s) 338
MspA1I CMGCKG 2 cut(s) 129, 429
MwoI GCNNNNNNNGC 2 cut(s) 179, 485
NdeII GATC 2 cut(s) 174, 420
NlaIII CATG 5 cut(s) 52, 172, 182, 383, 624
NlaIV GGNNCC 3 cut(s) 102, 103, 555
NspV TTCGAA 3 cut(s) 91, 234, 463
PciSI GCTCTTC 1 cut(s) 302
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 612
PfeI GAWTC 2 cut(s) 78, 473
PkrI GCNGC 3 cut(s) 365, 487, 601
PleI GAGTC 1 cut(s) 696
PpsI GAGTC 1 cut(s) 696
Ppu21I YACGTR 1 cut(s) 349
PpuMI RGGWCCY 1 cut(s) 101
Psp1406I AACGTT 1 cut(s) 441
Psp5II RGGWCCY 1 cut(s) 101
PspFI CCCAGC 1 cut(s) 96
PspN4I GGNNCC 3 cut(s) 102, 103, 555
PspPI GGNCC 2 cut(s) 101, 554
PspPPI RGGWCCY 1 cut(s) 101
PstI CTGCAG 1 cut(s) 163
PvuII CAGCTG 1 cut(s) 129
RsaI GTAC 2 cut(s) 347, 707
RsaNI GTAC 2 cut(s) 346, 706
RseI CAYNNNNRTG 1 cut(s) 398
SapI GCTCTTC 1 cut(s) 302
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 3 cut(s) 364, 486, 600
Sau3AI GATC 2 cut(s) 174, 420
Sau96I GGNCC 2 cut(s) 101, 554
SchI GAGTC 1 cut(s) 697
SfcI CTRYAG 1 cut(s) 159
SfuI TTCGAA 3 cut(s) 91, 234, 463
SinI GGWCC 1 cut(s) 101
SmiMI CAYNNNNRTG 1 cut(s) 398
Sse9I AATT 1 cut(s) 186
SsiI CCGC 4 cut(s) 364, 429, 545, 599
SspMI CTAG 2 cut(s) 75, 143
TaaI ACNGT 4 cut(s) 244, 345, 495, 535
TaiI ACGT 2 cut(s) 351, 444
TaqI TCGA 3 cut(s) 91, 234, 463
TasI AATT 1 cut(s) 186
TauI GCSGC 2 cut(s) 366, 602
TfiI GAWTC 2 cut(s) 78, 473
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TscAI CASTG 4 cut(s) 247, 348, 456, 538
TseI GCWGC 1 cut(s) 485
TspDTI ATGAA 4 cut(s) 157, 179, 310, 365
TspRI CASTG 4 cut(s) 247, 348, 456, 538
VpaK11BI GGWCC 1 cut(s) 101
XmnI GAANNNNTTC 1 cut(s) 612
XspI CTAG 2 cut(s) 75, 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.