FvH4_4g24350

TRAM, LAG1 and CLN8 homology domains.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004398
Physical Location & Seq
Forward (+)
26556821 .. 26557918
1098 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24350.t1

Sequence Viewer

Length: 741 bp
ATGGAGACCTCGGCTCTTTTCACCCCAACGCTCCCCACATTCTTCACCATGTTCTTGCTCATCTACCTCTTCGCTAGATTCTTGCTTTTTCGAACCTGGGGTCCCAAGCATAGGCCGGAAGCTTCGAGCTGCGTCATCTCTCTAGCTCACAGCACTCCTGCAGTTTTCATGGCGATCCATGCACTAATTCACAGCCAGAGAACAGCTTCCGTTTTCGCCTCCATGAACACACCTTTTGAAAACACTGTTCTTGAATTCAGCATAGCTTACTTCTTGGTTGATCTTCTTCATTACTTGATCTTCTTCCCTACTGAAATTCTCTTCATTCTTCATCACTTGGCCACACTGTACGTGTTTTCGACTTGTCGCTATGTGGTTCATCATGGGGCTTATGCCATCATTGTGCTTCTCTTTCTTGCCGAGATCACCAGCGGGTGCCAGAACGTTTGGACACTTGCGAGCTTTCAAAGGGCTGATTCGGCTTCGGCTGCGAAACTGTATGACTTCTTGTCTCCTCGGTTTTATGCTTTTTACAGTGTTTTTAGAGGGGTTCTGGGGCCTCTGTTTATGTTTAAGATGGGGTTGTTCTATATGAGTGGGGCGGCCGGAGAAGAAGTTCCGACATGGGCGTGGGTTTCTTGGATGGTCGTGATTGTTATTGCTATCTGTGTTAGCATATTGTGGGTGTTGAGTCATTGGAAAGATTGGTACAGAGACAAAGTTCAGAAGAAAGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAM_LAG1_CLN8

Protein Analysis

247

Amino Acids

28.29

Weight (kDa)

8.65

Isoelectric Point (pI)

32.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 42 - 231 2.7e-16 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 435
AciI CCGC 2 cut(s) 432, 602
AclI AACGTT 1 cut(s) 444
AclWI GGATC 1 cut(s) 169
AcoI YGGCCR 2 cut(s) 339, 603
AcsI RAATTY 2 cut(s) 254, 315
AfaI GTAC 2 cut(s) 350, 710
AfiI CCNNNNNNNGG 1 cut(s) 111
AflIII ACRYGT 1 cut(s) 351
AgsI TTSAA 3 cut(s) 239, 254, 467
AhdI GACNNNNNGTC 1 cut(s) 508
AjnI CCWGG 1 cut(s) 95
AloI GAACNNNNNNTCC 4 cut(s) 85, 117, 600, 632
AluBI AGCT 6 cut(s) 122, 129, 146, 206, 266, 462
AluI AGCT 6 cut(s) 122, 129, 146, 206, 266, 462
Alw26I GTCTC 2 cut(s) 516, 708
AlwI GGATC 1 cut(s) 169
AoxI GGCC 4 cut(s) 113, 339, 557, 603
ApeKI GCWGC 2 cut(s) 129, 488
ApoI RAATTY 2 cut(s) 254, 315
ArsI GACNNNNNNTTYG 2 cut(s) 85, 117
Asp700I GAANNNNTTC 2 cut(s) 205, 615
AspS9I GGNCC 2 cut(s) 101, 557
AsuHPI GGTGA 3 cut(s) 13, 37, 418
AsuII TTCGAA 1 cut(s) 91
AvaII GGWCC 1 cut(s) 101
BalI TGGCCA 1 cut(s) 341
BanI GGYRCC 1 cut(s) 435
BbvI GCAGC 2 cut(s) 116, 475
BccI CCATC 3 cut(s) 404, 571, 637
BciT130I CCWGG 1 cut(s) 97
BcoDI GTCTC 2 cut(s) 516, 708
BfaI CTAG 2 cut(s) 75, 143
BfmI CTRYAG 1 cut(s) 159
BisI GCNGC 3 cut(s) 130, 489, 603
BlsI GCNGC 3 cut(s) 131, 490, 604
Bme1390I CCNGG 1 cut(s) 97
Bme18I GGWCC 1 cut(s) 101
BmeRI GACNNNNNGTC 1 cut(s) 508
BmgT120I GGNCC 2 cut(s) 101, 557
BmiI GGNNCC 4 cut(s) 102, 103, 437, 558
BmrFI CCNGG 1 cut(s) 97
Bpu14I TTCGAA 1 cut(s) 91
BsaAI YACGTR 1 cut(s) 352
BsaJI CCNNGG 3 cut(s) 9, 96, 515
BsaXI ACNNNNNCTCC 2 cut(s) 600, 630
Bsc4I CCNNNNNNNGG 1 cut(s) 111
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 3 cut(s) 9, 96, 515
BseGI GGATG 1 cut(s) 648
BseLI CCNNNNNNNGG 1 cut(s) 111
BseRI GAGGAG 1 cut(s) 504
BseX3I CGGCCG 1 cut(s) 603
BseXI GCAGC 2 cut(s) 116, 475
Bsh1285I CGRYCG 1 cut(s) 606
BshFI GGCC 4 cut(s) 115, 341, 559, 605
BshNI GGYRCC 1 cut(s) 435
BsiEI CGRYCG 1 cut(s) 606
BsiSI CCGG 2 cut(s) 116, 606
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 2 cut(s) 516, 708
BsmFI GGGAC 1 cut(s) 87
BsnI GGCC 4 cut(s) 115, 341, 559, 605
Bsp119I TTCGAA 1 cut(s) 91
Bsp143I GATC 4 cut(s) 174, 280, 297, 423
BspACI CCGC 2 cut(s) 432, 602
BspANI GGCC 4 cut(s) 115, 341, 559, 605
BspLI GGNNCC 4 cut(s) 102, 103, 437, 558
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 1 cut(s) 169
BspT104I TTCGAA 1 cut(s) 91
BspT107I GGYRCC 1 cut(s) 435
BssECI CCNNGG 3 cut(s) 9, 96, 515
BssMI GATC 4 cut(s) 174, 280, 297, 423
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 4 cut(s) 247, 348, 498, 536
Bst6I CTCTTC 2 cut(s) 74, 326
BstBAI YACGTR 1 cut(s) 352
BstBI TTCGAA 1 cut(s) 91
BstC8I GCNNGC 1 cut(s) 460
BstF5I GGATG 1 cut(s) 648
BstKTI GATC 4 cut(s) 177, 283, 300, 426
BstMAI GTCTC 2 cut(s) 516, 708
BstMBI GATC 4 cut(s) 174, 280, 297, 423
BstMCI CGRYCG 1 cut(s) 606
BstMWI GCNNNNNNNGC 3 cut(s) 179, 479, 488
BstNI CCWGG 1 cut(s) 97
BstSCI CCNGG 1 cut(s) 95
BstSFI CTRYAG 1 cut(s) 159
BstV1I GCAGC 2 cut(s) 116, 475
BstZI CGGCCG 1 cut(s) 603
BsuRI GGCC 4 cut(s) 115, 341, 559, 605
BtsCI GGATG 1 cut(s) 648
BtsIMutI CAGTG 3 cut(s) 243, 344, 541
Cac8I GCNNGC 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 101, 557
CseI GACGC 1 cut(s) 121
Csp6I GTAC 2 cut(s) 349, 709
CviAII CATG 6 cut(s) 49, 169, 179, 223, 383, 624
CviQI GTAC 2 cut(s) 349, 709
DpnI GATC 4 cut(s) 176, 282, 299, 425
DpnII GATC 4 cut(s) 174, 280, 297, 423
DriI GACNNNNNGTC 1 cut(s) 508
EaeI YGGCCR 2 cut(s) 339, 603
EagI CGGCCG 1 cut(s) 603
Eam1104I CTCTTC 2 cut(s) 74, 326
Eam1105I GACNNNNNGTC 1 cut(s) 508
EarI CTCTTC 2 cut(s) 74, 326
EclXI CGGCCG 1 cut(s) 603
Eco47I GGWCC 1 cut(s) 101
Eco52I CGGCCG 1 cut(s) 603
EcoO109I RGGNCCY 2 cut(s) 101, 557
EcoRI GAATTC 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 95
FaeI CATG 6 cut(s) 52, 172, 182, 226, 386, 627
FaqI GGGAC 1 cut(s) 87
FatI CATG 6 cut(s) 48, 168, 178, 222, 382, 623
FauI CCCGC 1 cut(s) 425
Fnu4HI GCNGC 3 cut(s) 130, 489, 603
FokI GGATG 1 cut(s) 655
Fsp4HI GCNGC 3 cut(s) 130, 489, 603
FspBI CTAG 2 cut(s) 75, 143
GluI GCNGC 3 cut(s) 130, 489, 603
HaeIII GGCC 4 cut(s) 115, 341, 559, 605
HapII CCGG 2 cut(s) 116, 606
HgaI GACGC 1 cut(s) 121
Hin1II CATG 6 cut(s) 52, 172, 182, 226, 386, 627
HindIII AAGCTT 1 cut(s) 120
HinfI GANTC 3 cut(s) 78, 476, 691
HpaII CCGG 2 cut(s) 116, 606
HphI GGTGA 3 cut(s) 13, 37, 418
Hpy188I TCNGA 2 cut(s) 621, 726
Hpy188III TCNNGA 2 cut(s) 251, 649
HpyCH4III ACNGT 4 cut(s) 247, 348, 498, 536
HpyCH4IV ACGT 2 cut(s) 351, 444
HpyCH4V TGCA 2 cut(s) 161, 182
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 479, 488
HpySE526I ACGT 2 cut(s) 351, 444
Hsp92II CATG 6 cut(s) 52, 172, 182, 226, 386, 627
KflI GGGWCCC 1 cut(s) 101
Kzo9I GATC 4 cut(s) 174, 280, 297, 423
LmnI GCTCC 1 cut(s) 36
LpnPI CCDG 9 cut(s) 82, 109, 129, 171, 209, 442, 452, 539, 619
Lsp1109I GCAGC 2 cut(s) 116, 475
MaeI CTAG 2 cut(s) 75, 143
MaeII ACGT 2 cut(s) 351, 444
MalI GATC 4 cut(s) 176, 282, 299, 425
MboI GATC 4 cut(s) 174, 280, 297, 423
MlsI TGGCCA 1 cut(s) 341
MluCI AATT 3 cut(s) 186, 254, 315
MluNI TGGCCA 1 cut(s) 341
MlyI GAGTC 1 cut(s) 700
MmeI TCCRAC 1 cut(s) 644
MnlI CCTC 6 cut(s) 19, 77, 229, 525, 539, 570
Mox20I TGGCCA 1 cut(s) 341
MroXI GAANNNNTTC 2 cut(s) 205, 615
MscI TGGCCA 1 cut(s) 341
MseI TTAA 1 cut(s) 573
MslI CAYNNNNRTG 2 cut(s) 401, 628
Msp20I TGGCCA 1 cut(s) 341
MspA1I CMGCKG 1 cut(s) 432
MspI CCGG 2 cut(s) 116, 606
MspR9I CCNGG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 97
MwoI GCNNNNNNNGC 3 cut(s) 179, 479, 488
NdeII GATC 4 cut(s) 174, 280, 297, 423
NlaIII CATG 6 cut(s) 52, 172, 182, 226, 386, 627
NlaIV GGNNCC 4 cut(s) 102, 103, 437, 558
NmeAIII GCCGAG 1 cut(s) 445
NspV TTCGAA 1 cut(s) 91
PdmI GAANNNNTTC 2 cut(s) 205, 615
PfeI GAWTC 2 cut(s) 78, 476
PkrI GCNGC 3 cut(s) 131, 490, 604
PleI GAGTC 1 cut(s) 699
PpsI GAGTC 1 cut(s) 699
Ppu21I YACGTR 1 cut(s) 352
PpuMI RGGWCCY 1 cut(s) 101
Psp1406I AACGTT 1 cut(s) 444
Psp5II RGGWCCY 1 cut(s) 101
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 4 cut(s) 102, 103, 437, 558
PspPI GGNCC 2 cut(s) 101, 557
PspPPI RGGWCCY 1 cut(s) 101
PstI CTGCAG 1 cut(s) 163
RsaI GTAC 2 cut(s) 350, 710
RsaNI GTAC 2 cut(s) 349, 709
RseI CAYNNNNRTG 2 cut(s) 401, 628
SaqAI TTAA 1 cut(s) 573
SatI GCNGC 3 cut(s) 130, 489, 603
Sau3AI GATC 4 cut(s) 174, 280, 297, 423
Sau96I GGNCC 2 cut(s) 101, 557
SchI GAGTC 1 cut(s) 700
ScrFI CCNGG 1 cut(s) 97
SfcI CTRYAG 1 cut(s) 159
SfuI TTCGAA 1 cut(s) 91
SinI GGWCC 1 cut(s) 101
SmiMI CAYNNNNRTG 2 cut(s) 401, 628
Sse9I AATT 3 cut(s) 186, 254, 315
SsiI CCGC 2 cut(s) 432, 602
SspMI CTAG 2 cut(s) 75, 143
StyD4I CCNGG 1 cut(s) 95
TaaI ACNGT 4 cut(s) 247, 348, 498, 536
TaiI ACGT 2 cut(s) 354, 447
TaqI TCGA 3 cut(s) 91, 125, 359
TasI AATT 3 cut(s) 186, 254, 315
TauI GCSGC 1 cut(s) 605
TfiI GAWTC 2 cut(s) 78, 476
Tru1I TTAA 1 cut(s) 573
Tru9I TTAA 1 cut(s) 573
TscAI CASTG 3 cut(s) 250, 351, 541
TseI GCWGC 2 cut(s) 129, 488
TspDTI ATGAA 6 cut(s) 157, 239, 278, 313, 320, 368
TspGWI ACGGA 1 cut(s) 199
TspRI CASTG 3 cut(s) 250, 351, 541
VpaK11BI GGWCC 1 cut(s) 101
XapI RAATTY 2 cut(s) 254, 315
XmnI GAANNNNTTC 2 cut(s) 205, 615
XspI CTAG 2 cut(s) 75, 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.