pycom17g01340

TRAM, LAG1 and CLN8 homology domains.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004398
Physical Location & Seq
Forward (+)
907479 .. 908564
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g01340.1

Sequence Viewer

Length: 1026 bp
ATGTATCCAAAACACGAAATTCCTATTACCTATAAAATAACATATAACTTACTGTTCATGTTTCCGAGATTTTTCAATATACCCAAAACACGAAATCACTTCATACAAATAAACAATAAAATAGATCAGTCTCAAGAGTCGCACTCACACACCCACACCACACCAGTCGTCGTCCCACTCTCCATCCCAAACAACACAATTCTCTATAAATTAAACCCTCTCTCCGATTCCCGTAATCTTCGTCAAAGCCCTTTGATTTCGTTTCCATCTCAGATGGAAACCCAAAACCCAATCCACTCCCCGCCATCGCCGTCGCTGTCACCGTCGTCATCTCTCCAACTCCCACGCCTCCCCATCTTCTTCCTCATGTTCTCTATCGTCTACGTCATTGCCTACTTCATCGTCTTCCGAACGTGGAGCCCTAAGATCCGACCCGAAGCCTCCAGCTGCCTAATCTCCCTCGCCCACGGCACCCCCGCCGTCTTTCTCTCCGCCTACGCCATCCTCTCCGACCCCGCCACCGGATTCGCCGCCCCCAACACCCTTTTCCAGAACTCCGTCCTCGATTACAGCGTCGCCTACTTCCTCACCGATCTCCTCCACTACCTCGTCTTCTTCCCCAGCGACGTCCTCTTCATCGGCCACCACCTCGCCACGCTCTTCGTCTTCCTCACCTGCCGCTACGTCGCCTCCCACGGCGCATTCGCCATTCTCTGCCTCCTGATCCTCGCCGAGGTCACCAGCCTCTGCCAGAACGTCTGGACGCTCGCCAACGCCAGGAGAGGCGACTTGAAGTTTGCAGCTAAGGTGTATCGATCTTTTGTCTCCTCCTTTTTACATTCTGTATTCGATTGTTCGTGGCTTTGTGGGTCCGTATTTCGTGTACCGAATGGGGGCCTTTTACATCAGCGGCGCCGCCGACGGATTGATTCCGCGGTGGGTTTGGGGTTCCTGGATTGTGGTAGTGATGGCCGCCATTTCTGTCAGCATTTTGTGGATTTCGAATCTCTGGGTCGAATTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573 TRAM_LAG1_CLN8

Protein Analysis

342

Amino Acids

38.58

Weight (kDa)

9.23

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 148 - 276 6.9e-11 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 683
AatII GACGTC 1 cut(s) 630
Acc36I ACCTGC 1 cut(s) 683
AccB1I GGYRCC 2 cut(s) 470, 912
AccI GTMKAC 1 cut(s) 381
AccII CGCG 1 cut(s) 935
AclWI GGATC 2 cut(s) 421, 718
AcoI YGGCCR 2 cut(s) 640, 970
AcsI RAATTY 1 cut(s) 18
AcyI GRCGYC 2 cut(s) 627, 913
AfaI GTAC 1 cut(s) 885
AfiI CCNNNNNNNGG 4 cut(s) 521, 733, 777, 893
AgsI TTSAA 2 cut(s) 76, 793
AjnI CCWGG 2 cut(s) 776, 951
AjuI GAANNNNNNNTTGG 2 cut(s) 530, 562
AluBI AGCT 2 cut(s) 447, 803
AluI AGCT 2 cut(s) 447, 803
Alw26I GTCTC 2 cut(s) 135, 829
AlwI GGATC 2 cut(s) 421, 718
AlwNI CAGNNNCTG 1 cut(s) 747
AoxI GGCC 3 cut(s) 640, 895, 970
ApeKI GCWGC 2 cut(s) 447, 800
ApoI RAATTY 1 cut(s) 18
ArsI GACNNNNNNTTYG 2 cut(s) 779, 811
AspLEI GCGC 2 cut(s) 701, 915
AspS9I GGNCC 2 cut(s) 870, 895
AsuHPI GGTGA 4 cut(s) 312, 580, 664, 730
AsuII TTCGAA 1 cut(s) 1002
AvaII GGWCC 1 cut(s) 870
BanI GGYRCC 2 cut(s) 470, 912
BanII GRGCYC 1 cut(s) 422
BbsI GAAGAC 3 cut(s) 397, 604, 658
BbvI GCAGC 2 cut(s) 434, 812
BccI CCATC 7 cut(s) 191, 268, 274, 313, 362, 509, 962
BceAI ACGGC 4 cut(s) 295, 464, 484, 712
BciT130I CCWGG 2 cut(s) 778, 953
BciVI GTATCC 1 cut(s) 15
BcoDI GTCTC 2 cut(s) 135, 829
BfoI RGCGCY 1 cut(s) 916
BfuAI ACCTGC 1 cut(s) 683
BfuI GTATCC 1 cut(s) 15
BisI GCNGC 7 cut(s) 448, 531, 679, 801, 911, 916, 973
BlsI GCNGC 7 cut(s) 449, 532, 680, 802, 912, 917, 974
Bme1390I CCNGG 2 cut(s) 778, 953
Bme18I GGWCC 1 cut(s) 870
BmgT120I GGNCC 2 cut(s) 870, 895
BmiI GGNNCC 6 cut(s) 419, 472, 871, 896, 914, 950
BmrFI CCNGG 2 cut(s) 778, 953
BpiI GAAGAC 3 cut(s) 397, 604, 658
BplI GAGNNNNNCTC 2 cut(s) 128, 160
BpmI CTGGAG 1 cut(s) 427
Bpu10I CCTNAGC 1 cut(s) 804
Bpu14I TTCGAA 1 cut(s) 1002
BpuEI CTTGAG 1 cut(s) 117
Bsa29I ATCGAT 1 cut(s) 814
BsaHI GRCGYC 2 cut(s) 627, 913
BsaJI CCNNGG 4 cut(s) 466, 694, 732, 933
BsaWI WCCGGW 1 cut(s) 521
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 674, 704
Bsc4I CCNNNNNNNGG 4 cut(s) 521, 733, 777, 893
Bse1I ACTGG 1 cut(s) 164
Bse3DI GCAATG 1 cut(s) 387
BseBI CCWGG 2 cut(s) 778, 953
BseCI ATCGAT 1 cut(s) 814
BseDI CCNNGG 4 cut(s) 466, 694, 732, 933
BseGI GGATG 2 cut(s) 183, 501
BseLI CCNNNNNNNGG 4 cut(s) 521, 733, 777, 893
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 1 cut(s) 284
BseNI ACTGG 1 cut(s) 164
BseRI GAGGAG 2 cut(s) 587, 817
BseXI GCAGC 2 cut(s) 434, 812
BseYI CCCAGC 1 cut(s) 620
Bsh1236I CGCG 1 cut(s) 935
BshFI GGCC 3 cut(s) 642, 897, 972
BshNI GGYRCC 2 cut(s) 470, 912
BshVI ATCGAT 1 cut(s) 814
BsiSI CCGG 1 cut(s) 522
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 4 cut(s) 521, 733, 777, 893
BsmAI GTCTC 2 cut(s) 135, 829
BsmFI GGGAC 1 cut(s) 158
BsmI GAATGC 1 cut(s) 701
BsnI GGCC 3 cut(s) 642, 897, 972
Bsp119I TTCGAA 1 cut(s) 1002
Bsp1286I GDGCHC 1 cut(s) 422
Bsp143I GATC 5 cut(s) 124, 426, 592, 723, 815
BspANI GGCC 3 cut(s) 642, 897, 972
BspCNI CTCAG 1 cut(s) 283
BspDI ATCGAT 1 cut(s) 814
BspFNI CGCG 1 cut(s) 935
BspLI GGNNCC 6 cut(s) 419, 472, 871, 896, 914, 950
BspMI ACCTGC 1 cut(s) 683
BspPI GGATC 2 cut(s) 421, 718
BspQI GCTCTTC 1 cut(s) 665
BspT104I TTCGAA 1 cut(s) 1002
BspT107I GGYRCC 2 cut(s) 470, 912
BsrDI GCAATG 1 cut(s) 387
BsrI ACTGG 1 cut(s) 164
BssECI CCNNGG 4 cut(s) 466, 694, 732, 933
BssMI GATC 5 cut(s) 124, 426, 592, 723, 815
BssNI GRCGYC 2 cut(s) 627, 913
Bst2UI CCWGG 2 cut(s) 778, 953
Bst4CI ACNGT 2 cut(s) 54, 324
Bst6I CTCTTC 2 cut(s) 638, 665
BstACI GRCGYC 2 cut(s) 627, 913
BstBI TTCGAA 1 cut(s) 1002
BstC8I GCNNGC 1 cut(s) 768
BstDEI CTNAG 3 cut(s) 270, 423, 804
BstDSI CCRYGG 3 cut(s) 466, 694, 933
BstEII GGTNACC 1 cut(s) 736
BstENI CCTNNNNNAGG 1 cut(s) 731
BstF5I GGATG 2 cut(s) 183, 501
BstFNI CGCG 1 cut(s) 935
BstH2I RGCGCY 1 cut(s) 916
BstHHI GCGC 2 cut(s) 701, 915
BstKTI GATC 5 cut(s) 127, 429, 595, 726, 818
BstMAI GTCTC 2 cut(s) 135, 829
BstMBI GATC 5 cut(s) 124, 426, 592, 723, 815
BstNI CCWGG 2 cut(s) 778, 953
BstPI GGTNACC 1 cut(s) 736
BstSCI CCNGG 2 cut(s) 776, 951
BstUI CGCG 1 cut(s) 935
BstV1I GCAGC 2 cut(s) 434, 812
BstV2I GAAGAC 3 cut(s) 397, 604, 658
BstX2I RGATCY 1 cut(s) 426
BstYI RGATCY 1 cut(s) 426
Bsu15I ATCGAT 1 cut(s) 814
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 3 cut(s) 642, 897, 972
BsuTUI ATCGAT 1 cut(s) 814
BtgI CCRYGG 3 cut(s) 466, 694, 933
BtgZI GCGATG 1 cut(s) 291
BtsCI GGATG 2 cut(s) 183, 501
BveI ACCTGC 1 cut(s) 683
Cac8I GCNNGC 1 cut(s) 768
CaiI CAGNNNCTG 1 cut(s) 747
CfoI GCGC 2 cut(s) 701, 915
Cfr13I GGNCC 2 cut(s) 870, 895
Cfr42I CCGCGG 1 cut(s) 936
ClaI ATCGAT 1 cut(s) 814
CseI GACGC 2 cut(s) 562, 772
Csp6I GTAC 1 cut(s) 884
CviAII CATG 2 cut(s) 58, 367
CviQI GTAC 1 cut(s) 884
DdeI CTNAG 3 cut(s) 270, 423, 804
DinI GGCGCC 1 cut(s) 914
DpnI GATC 5 cut(s) 126, 428, 594, 725, 817
DpnII GATC 5 cut(s) 124, 426, 592, 723, 815
EaeI YGGCCR 2 cut(s) 640, 970
Eam1104I CTCTTC 2 cut(s) 638, 665
EarI CTCTTC 2 cut(s) 638, 665
EciI GGCGGA 1 cut(s) 481
Eco24I GRGCYC 1 cut(s) 422
Eco47I GGWCC 1 cut(s) 870
Eco91I GGTNACC 1 cut(s) 736
EcoNI CCTNNNNNAGG 1 cut(s) 731
EcoO109I RGGNCCY 1 cut(s) 895
EcoO65I GGTNACC 1 cut(s) 736
EcoRII CCWGG 2 cut(s) 776, 951
EcoT38I GRGCYC 1 cut(s) 422
EgeI GGCGCC 1 cut(s) 914
EheI GGCGCC 1 cut(s) 914
FaeI CATG 2 cut(s) 61, 370
FaiI YATR 8 cut(s) 33, 43, 45, 59, 80, 104, 207, 368
FaqI GGGAC 1 cut(s) 158
FatI CATG 2 cut(s) 57, 366
FauI CCCGC 3 cut(s) 309, 484, 523
FblI GTMKAC 1 cut(s) 381
Fnu4HI GCNGC 7 cut(s) 448, 531, 679, 801, 911, 916, 973
FokI GGATG 2 cut(s) 170, 488
FriOI GRGCYC 1 cut(s) 422
Fsp4HI GCNGC 7 cut(s) 448, 531, 679, 801, 911, 916, 973
GlaI GCGC 2 cut(s) 700, 914
GluI GCNGC 7 cut(s) 448, 531, 679, 801, 911, 916, 973
GsaI CCCAGC 1 cut(s) 624
GsuI CTGGAG 1 cut(s) 427
HaeII RGCGCY 1 cut(s) 916
HaeIII GGCC 3 cut(s) 642, 897, 972
HapII CCGG 1 cut(s) 522
HgaI GACGC 2 cut(s) 562, 772
HhaI GCGC 2 cut(s) 701, 915
Hin1I GRCGYC 2 cut(s) 627, 913
Hin1II CATG 2 cut(s) 61, 370
Hin6I GCGC 2 cut(s) 699, 913
HinP1I GCGC 2 cut(s) 699, 913
HinfI GANTC 5 cut(s) 137, 227, 525, 929, 1004
HpaII CCGG 1 cut(s) 522
HphI GGTGA 4 cut(s) 312, 580, 664, 730
Hpy166II GTNNAC 2 cut(s) 382, 884
Hpy188I TCNGA 6 cut(s) 66, 226, 273, 410, 431, 511
Hpy188III TCNNGA 4 cut(s) 134, 550, 721, 760
Hpy8I GTNNAC 2 cut(s) 382, 884
Hpy99I CGWCG 7 cut(s) 173, 316, 328, 578, 629, 689, 924
HpyCH4III ACNGT 2 cut(s) 54, 324
HpyCH4IV ACGT 5 cut(s) 384, 413, 627, 684, 756
HpyCH4V TGCA 1 cut(s) 800
HpyF3I CTNAG 3 cut(s) 270, 423, 804
HpySE526I ACGT 5 cut(s) 384, 413, 627, 684, 756
Hsp92I GRCGYC 2 cut(s) 627, 913
Hsp92II CATG 2 cut(s) 61, 370
HspAI GCGC 2 cut(s) 699, 913
KasI GGCGCC 1 cut(s) 912
KspI CCGCGG 1 cut(s) 936
Kzo9I GATC 5 cut(s) 124, 426, 592, 723, 815
LguI GCTCTTC 1 cut(s) 665
LmnI GCTCC 1 cut(s) 417
Lsp1109I GCAGC 2 cut(s) 434, 812
MaeII ACGT 5 cut(s) 384, 413, 627, 684, 756
MaeIII GTNAC 2 cut(s) 318, 736
MalI GATC 5 cut(s) 126, 428, 594, 725, 817
MboI GATC 5 cut(s) 124, 426, 592, 723, 815
MboII GAAGA 9 cut(s) 230, 349, 352, 397, 604, 607, 625, 652, 658
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 1 cut(s) 422
MluCI AATT 4 cut(s) 18, 198, 209, 1017
Mly113I GGCGCC 1 cut(s) 913
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 3 cut(s) 361, 454, 534
MseI TTAA 1 cut(s) 212
MspA1I CMGCKG 3 cut(s) 447, 910, 935
MspI CCGG 1 cut(s) 522
MspR9I CCNGG 2 cut(s) 778, 953
Mva1269I GAATGC 1 cut(s) 701
MvaI CCWGG 2 cut(s) 778, 953
MvnI CGCG 1 cut(s) 935
NarI GGCGCC 1 cut(s) 913
NdeII GATC 5 cut(s) 124, 426, 592, 723, 815
NlaIII CATG 2 cut(s) 61, 370
NlaIV GGNNCC 6 cut(s) 419, 472, 871, 896, 914, 950
NmeAIII GCCGAG 1 cut(s) 757
NmuCI GTSAC 2 cut(s) 318, 736
NspV TTCGAA 1 cut(s) 1002
PaqCI CACCTGC 1 cut(s) 683
PciSI GCTCTTC 1 cut(s) 665
PcsI WCGNNNNNNNCGW 2 cut(s) 320, 570
PctI GAATGC 1 cut(s) 701
PfeI GAWTC 4 cut(s) 227, 525, 929, 1004
PfoI TCCNGGA 1 cut(s) 951
PkrI GCNGC 7 cut(s) 449, 532, 680, 802, 912, 917, 974
PleI GAGTC 1 cut(s) 145
PluTI GGCGCC 1 cut(s) 916
PpsI GAGTC 1 cut(s) 145
Psp6I CCWGG 2 cut(s) 776, 951
PspEI GGTNACC 1 cut(s) 736
PspFI CCCAGC 1 cut(s) 620
PspGI CCWGG 2 cut(s) 776, 951
PspN4I GGNNCC 6 cut(s) 419, 472, 871, 896, 914, 950
PspPI GGNCC 2 cut(s) 870, 895
PstNI CAGNNNCTG 1 cut(s) 747
PsuI RGATCY 1 cut(s) 426
PvuII CAGCTG 1 cut(s) 447
RsaI GTAC 1 cut(s) 885
RsaNI GTAC 1 cut(s) 884
SacII CCGCGG 1 cut(s) 936
SapI GCTCTTC 1 cut(s) 665
SaqAI TTAA 1 cut(s) 212
SatI GCNGC 7 cut(s) 448, 531, 679, 801, 911, 916, 973
Sau3AI GATC 5 cut(s) 124, 426, 592, 723, 815
Sau96I GGNCC 2 cut(s) 870, 895
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 2 cut(s) 778, 953
SduI GDGCHC 1 cut(s) 422
SfoI GGCGCC 1 cut(s) 914
Sfr303I CCGCGG 1 cut(s) 936
SfuI TTCGAA 1 cut(s) 1002
SgrBI CCGCGG 1 cut(s) 936
SinI GGWCC 1 cut(s) 870
SmlI CTYRAG 1 cut(s) 132
SmoI CTYRAG 1 cut(s) 132
Sse9I AATT 4 cut(s) 18, 198, 209, 1017
SspDI GGCGCC 1 cut(s) 912
StyD4I CCNGG 2 cut(s) 776, 951
TaaI ACNGT 2 cut(s) 54, 324
TaiI ACGT 5 cut(s) 387, 416, 630, 687, 759
TaqI TCGA 5 cut(s) 564, 814, 849, 1002, 1015
TasI AATT 4 cut(s) 18, 198, 209, 1017
TauI GCSGC 5 cut(s) 533, 681, 913, 918, 975
TfiI GAWTC 4 cut(s) 227, 525, 929, 1004
Tru1I TTAA 1 cut(s) 212
Tru9I TTAA 1 cut(s) 212
TseFI GTSAC 2 cut(s) 318, 736
TseI GCWGC 2 cut(s) 447, 800
Tsp45I GTSAC 2 cut(s) 318, 736
TspDTI ATGAA 4 cut(s) 46, 91, 388, 625
TspGWI ACGGA 3 cut(s) 547, 862, 937
VpaK11BI GGWCC 1 cut(s) 870
XagI CCTNNNNNAGG 1 cut(s) 731
XapI RAATTY 1 cut(s) 18
XmiI GTMKAC 1 cut(s) 381
ZraI GACGTC 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.