Rw2G054000

TRAM, LAG1 and CLN8 homology domains.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004398
Physical Location & Seq
Reverse (-)
81733855 .. 81735241
1387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G054000.1

Sequence Viewer

Length: 756 bp
ATGGGAACGCAGCAAAGCCCAATTCTTTCTTCCCTCCCACCCCTCCCAATCCTCTTCTCCTTCTTCTTGACCGTCTACCTCATCGCCTACTTCCTCGTCTTCCGCTCCTGGAGCCCCAAGATCCGACCCGAAGCCTCCAGCTGCCTCATCTCCCTCGCCCACGGCACCCCCGCCGTCTTCTTCTCCTCCTTCGCCATTCTCTCCGATCCCTCCACCGGCTTCGCCGCCCCCAACACGCGCCTCCAGAACTCCGTCCTCGATTTCAGCGTCGCCTATTTCCTCATGGATCTTCTCCACTACCTCCTCTTCTTCCCCGGCGACGTCCTCTTCATCGGCCACCACCTCGCCACGCTCTTCGTCTTCCTCACCTGCCGCTACGTCGCCTCCCGCGGCGCCTTCGCCGTCCTCACGCTCCTCATCCTCGCCGAGGTCACCAGCCTCTGCCAGAACGTCTGGACGCTCGCCAACGCGCGCCGGAGGGACGTGGAGTTCGCCGCCAAGGTGTACGATTTTCTGTCTCCTCCGTTTTATGTTCTCTATTCGATTGTGAGGGGCTTTGTTGGCCCTTGTTTCGTGTATAGAATGTGTGCTTTCTACGCTAGCGGCGCCGCCGAGGGCTTGATTCCCCGGTGGGTTTGGGTTTCGTGGATTGTGGTGGTAATGTCTGCTATTTCTGTTAGCATTATGTGGATTTCGAATCTTTGGGTGGAGTTGTATAGAGATAGGAGTGGCAAGTTGGAAAAGAAAGTTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573 TRAM_LAG1_CLN8

Protein Analysis

251

Amino Acids

28.25

Weight (kDa)

9.01

Isoelectric Point (pI)

41.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 46 - 235 1.9e-18 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 377
AatII GACGTC 1 cut(s) 324
Acc36I ACCTGC 1 cut(s) 377
AccB1I GGYRCC 3 cut(s) 164, 392, 605
AccBSI CCGCTC 1 cut(s) 105
AccI GTMKAC 1 cut(s) 75
AccII CGCG 4 cut(s) 238, 390, 470, 472
AciI CCGC 9 cut(s) 103, 171, 225, 373, 388, 390, 495, 603, 609
AclWI GGATC 3 cut(s) 115, 200, 294
AcoI YGGCCR 1 cut(s) 334
AcyI GRCGYC 3 cut(s) 321, 393, 606
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 2 cut(s) 215, 427
AjiI CACGTC 1 cut(s) 484
AjnI CCWGG 1 cut(s) 107
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AloI GAACNNNNNNTCC 2 cut(s) 473, 505
AluBI AGCT 1 cut(s) 141
AluI AGCT 1 cut(s) 141
Alw26I GTCTC 1 cut(s) 522
AlwI GGATC 3 cut(s) 115, 200, 294
AlwNI CAGNNNCTG 1 cut(s) 441
AoxI GGCC 2 cut(s) 334, 562
ApeKI GCWGC 2 cut(s) 10, 141
ArsI GACNNNNNNTTYG 2 cut(s) 473, 505
AspLEI GCGC 5 cut(s) 240, 395, 472, 474, 608
AspS9I GGNCC 1 cut(s) 563
AsuC2I CCSGG 2 cut(s) 315, 628
AsuHPI GGTGA 2 cut(s) 358, 424
AsuII TTCGAA 1 cut(s) 695
AsuNHI GCTAGC 1 cut(s) 599
BanI GGYRCC 3 cut(s) 164, 392, 605
BanII GRGCYC 1 cut(s) 116
BbsI GAAGAC 3 cut(s) 91, 169, 352
BbvI GCAGC 2 cut(s) 22, 128
BceAI ACGGC 3 cut(s) 158, 178, 386
BciT130I CCWGG 1 cut(s) 109
BcnI CCSGG 2 cut(s) 315, 628
BcoDI GTCTC 1 cut(s) 522
BfaI CTAG 1 cut(s) 600
BfoI RGCGCY 2 cut(s) 396, 609
BfuAI ACCTGC 1 cut(s) 377
BisI GCNGC 8 cut(s) 11, 142, 225, 373, 391, 495, 604, 609
BlsI GCNGC 8 cut(s) 12, 143, 226, 374, 392, 496, 605, 610
Bme1390I CCNGG 3 cut(s) 109, 315, 628
BmgBI CACGTC 1 cut(s) 484
BmgT120I GGNCC 1 cut(s) 563
BmiI GGNNCC 4 cut(s) 113, 166, 394, 607
BmrFI CCNGG 3 cut(s) 109, 315, 628
BmtI GCTAGC 1 cut(s) 603
BpiI GAAGAC 3 cut(s) 91, 169, 352
BpmI CTGGAG 3 cut(s) 121, 130, 227
Bpu14I TTCGAA 1 cut(s) 695
BpuMI CCSGG 2 cut(s) 315, 628
BsaHI GRCGYC 3 cut(s) 321, 393, 606
BsaJI CCNNGG 7 cut(s) 160, 313, 388, 426, 498, 612, 626
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 427
Bse118I RCCGGY 1 cut(s) 215
BseBI CCWGG 1 cut(s) 109
BseDI CCNNGG 7 cut(s) 160, 313, 388, 426, 498, 612, 626
BseGI GGATG 1 cut(s) 417
BseLI CCNNNNNNNGG 2 cut(s) 215, 427
BsePI GCGCGC 1 cut(s) 470
BseRI GAGGAG 4 cut(s) 175, 293, 404, 510
BseXI GCAGC 2 cut(s) 22, 128
Bsh1236I CGCG 4 cut(s) 238, 390, 470, 472
BshFI GGCC 2 cut(s) 336, 564
BshNI GGYRCC 3 cut(s) 164, 392, 605
BsiSI CCGG 4 cut(s) 216, 315, 475, 628
BslFI GGGAC 1 cut(s) 494
BslI CCNNNNNNNGG 2 cut(s) 215, 427
BsmAI GTCTC 1 cut(s) 522
BsmFI GGGAC 1 cut(s) 494
BsnI GGCC 2 cut(s) 336, 564
Bsp119I TTCGAA 1 cut(s) 695
Bsp1286I GDGCHC 1 cut(s) 116
Bsp143I GATC 3 cut(s) 120, 205, 286
BspACI CCGC 9 cut(s) 103, 171, 225, 373, 388, 390, 495, 603, 609
BspANI GGCC 2 cut(s) 336, 564
BspFNI CGCG 4 cut(s) 238, 390, 470, 472
BspLI GGNNCC 4 cut(s) 113, 166, 394, 607
BspMI ACCTGC 1 cut(s) 377
BspOI GCTAGC 1 cut(s) 603
BspPI GGATC 3 cut(s) 115, 200, 294
BspQI GCTCTTC 1 cut(s) 359
BspT104I TTCGAA 1 cut(s) 695
BspT107I GGYRCC 3 cut(s) 164, 392, 605
BsrBI CCGCTC 1 cut(s) 105
BsrFI RCCGGY 1 cut(s) 215
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 7 cut(s) 160, 313, 388, 426, 498, 612, 626
BssHII GCGCGC 1 cut(s) 470
BssMI GATC 3 cut(s) 120, 205, 286
BssNI GRCGYC 3 cut(s) 321, 393, 606
BssT1I CCWWGG 1 cut(s) 498
Bst2UI CCWGG 1 cut(s) 109
Bst4CI ACNGT 1 cut(s) 73
Bst6I CTCTTC 4 cut(s) 59, 311, 332, 359
BstACI GRCGYC 3 cut(s) 321, 393, 606
BstBI TTCGAA 1 cut(s) 695
BstC8I GCNNGC 3 cut(s) 462, 472, 601
BstDSI CCRYGG 2 cut(s) 160, 388
BstEII GGTNACC 1 cut(s) 430
BstENI CCTNNNNNAGG 1 cut(s) 425
BstF5I GGATG 1 cut(s) 417
BstFNI CGCG 4 cut(s) 238, 390, 470, 472
BstH2I RGCGCY 2 cut(s) 396, 609
BstHHI GCGC 5 cut(s) 240, 395, 472, 474, 608
BstKTI GATC 3 cut(s) 123, 208, 289
BstMAI GTCTC 1 cut(s) 522
BstMBI GATC 3 cut(s) 120, 205, 286
BstMWI GCNNNNNNNGC 4 cut(s) 111, 561, 596, 605
BstNI CCWGG 1 cut(s) 109
BstPI GGTNACC 1 cut(s) 430
BstSCI CCNGG 3 cut(s) 107, 313, 626
BstUI CGCG 4 cut(s) 238, 390, 470, 472
BstV1I GCAGC 2 cut(s) 22, 128
BstV2I GAAGAC 3 cut(s) 91, 169, 352
BstX2I RGATCY 2 cut(s) 120, 286
BstYI RGATCY 2 cut(s) 120, 286
BsuRI GGCC 2 cut(s) 336, 564
BtgI CCRYGG 2 cut(s) 160, 388
BtgZI GCGATG 1 cut(s) 67
BtrI CACGTC 1 cut(s) 484
BtsCI GGATG 1 cut(s) 417
BveI ACCTGC 1 cut(s) 377
Cac8I GCNNGC 3 cut(s) 462, 472, 601
CaiI CAGNNNCTG 1 cut(s) 441
CfoI GCGC 5 cut(s) 240, 395, 472, 474, 608
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 563
Cfr42I CCGCGG 1 cut(s) 391
CseI GACGC 2 cut(s) 256, 466
Csp6I GTAC 1 cut(s) 505
CviAII CATG 1 cut(s) 283
CviQI GTAC 1 cut(s) 505
DinI GGCGCC 2 cut(s) 394, 607
DpnI GATC 3 cut(s) 122, 207, 288
DpnII GATC 3 cut(s) 120, 205, 286
EaeI YGGCCR 1 cut(s) 334
Eam1104I CTCTTC 4 cut(s) 59, 311, 332, 359
EarI CTCTTC 4 cut(s) 59, 311, 332, 359
Eco130I CCWWGG 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 116
Eco91I GGTNACC 1 cut(s) 430
EcoNI CCTNNNNNAGG 1 cut(s) 425
EcoO65I GGTNACC 1 cut(s) 430
EcoRII CCWGG 1 cut(s) 107
EcoT14I CCWWGG 1 cut(s) 498
EcoT38I GRGCYC 1 cut(s) 116
EgeI GGCGCC 2 cut(s) 394, 607
EheI GGCGCC 2 cut(s) 394, 607
ErhI CCWWGG 1 cut(s) 498
FaeI CATG 1 cut(s) 286
FaiI YATR 6 cut(s) 284, 531, 579, 686, 717, 754
FaqI GGGAC 1 cut(s) 494
FatI CATG 1 cut(s) 282
FauI CCCGC 2 cut(s) 178, 395
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 8 cut(s) 11, 142, 225, 373, 391, 495, 604, 609
FokI GGATG 1 cut(s) 404
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 8 cut(s) 11, 142, 225, 373, 391, 495, 604, 609
FspBI CTAG 1 cut(s) 600
GlaI GCGC 5 cut(s) 239, 394, 471, 473, 607
GluI GCNGC 8 cut(s) 11, 142, 225, 373, 391, 495, 604, 609
GsuI CTGGAG 3 cut(s) 121, 130, 227
HaeII RGCGCY 2 cut(s) 396, 609
HaeIII GGCC 2 cut(s) 336, 564
HapII CCGG 4 cut(s) 216, 315, 475, 628
HgaI GACGC 2 cut(s) 256, 466
HhaI GCGC 5 cut(s) 240, 395, 472, 474, 608
Hin1I GRCGYC 3 cut(s) 321, 393, 606
Hin1II CATG 1 cut(s) 286
Hin6I GCGC 5 cut(s) 238, 393, 470, 472, 606
HinP1I GCGC 5 cut(s) 238, 393, 470, 472, 606
HinfI GANTC 2 cut(s) 622, 697
HpaII CCGG 4 cut(s) 216, 315, 475, 628
HphI GGTGA 2 cut(s) 358, 424
Hpy166II GTNNAC 2 cut(s) 76, 505
Hpy188I TCNGA 2 cut(s) 125, 205
Hpy188III TCNNGA 3 cut(s) 67, 244, 454
Hpy8I GTNNAC 2 cut(s) 76, 505
Hpy99I CGWCG 3 cut(s) 272, 323, 383
HpyAV CCTTC 3 cut(s) 70, 199, 406
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4IV ACGT 4 cut(s) 321, 378, 450, 483
HpyCH4V TGCA 1 cut(s) 752
HpyF10VI GCNNNNNNNGC 4 cut(s) 111, 561, 596, 605
HpySE526I ACGT 4 cut(s) 321, 378, 450, 483
Hsp92I GRCGYC 3 cut(s) 321, 393, 606
Hsp92II CATG 1 cut(s) 286
HspAI GCGC 5 cut(s) 238, 393, 470, 472, 606
KasI GGCGCC 2 cut(s) 392, 605
KspI CCGCGG 1 cut(s) 391
Kzo9I GATC 3 cut(s) 120, 205, 286
LguI GCTCTTC 1 cut(s) 359
LmnI GCTCC 3 cut(s) 110, 111, 417
Lsp1109I GCAGC 2 cut(s) 22, 128
MaeI CTAG 1 cut(s) 600
MaeII ACGT 4 cut(s) 321, 378, 450, 483
MaeIII GTNAC 1 cut(s) 430
MalI GATC 3 cut(s) 122, 207, 288
MbiI CCGCTC 1 cut(s) 105
MboI GATC 3 cut(s) 120, 205, 286
MflI RGATCY 2 cut(s) 120, 286
MhlI GDGCHC 1 cut(s) 116
MluCI AATT 1 cut(s) 21
Mly113I GGCGCC 2 cut(s) 393, 606
MmeI TCCRAC 2 cut(s) 148, 717
MspA1I CMGCKG 2 cut(s) 141, 390
MspI CCGG 4 cut(s) 216, 315, 475, 628
MspR9I CCNGG 3 cut(s) 109, 315, 628
MvaI CCWGG 1 cut(s) 109
MvnI CGCG 4 cut(s) 238, 390, 470, 472
MwoI GCNNNNNNNGC 4 cut(s) 111, 561, 596, 605
NarI GGCGCC 2 cut(s) 393, 606
NciI CCSGG 2 cut(s) 315, 628
NdeII GATC 3 cut(s) 120, 205, 286
NheI GCTAGC 1 cut(s) 599
NlaIII CATG 1 cut(s) 286
NlaIV GGNNCC 4 cut(s) 113, 166, 394, 607
NmeAIII GCCGAG 2 cut(s) 451, 637
NmuCI GTSAC 1 cut(s) 430
NspV TTCGAA 1 cut(s) 695
PaqCI CACCTGC 1 cut(s) 377
PauI GCGCGC 1 cut(s) 470
PciSI GCTCTTC 1 cut(s) 359
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PfeI GAWTC 2 cut(s) 622, 697
PfoI TCCNGGA 1 cut(s) 107
PkrI GCNGC 8 cut(s) 12, 143, 226, 374, 392, 496, 605, 610
PluTI GGCGCC 2 cut(s) 396, 609
Psp6I CCWGG 1 cut(s) 107
PspEI GGTNACC 1 cut(s) 430
PspGI CCWGG 1 cut(s) 107
PspN4I GGNNCC 4 cut(s) 113, 166, 394, 607
PspPI GGNCC 1 cut(s) 563
PstNI CAGNNNCTG 1 cut(s) 441
PsuI RGATCY 2 cut(s) 120, 286
PteI GCGCGC 1 cut(s) 470
PvuII CAGCTG 1 cut(s) 141
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
SacII CCGCGG 1 cut(s) 391
SapI GCTCTTC 1 cut(s) 359
SatI GCNGC 8 cut(s) 11, 142, 225, 373, 391, 495, 604, 609
Sau3AI GATC 3 cut(s) 120, 205, 286
Sau96I GGNCC 1 cut(s) 563
ScrFI CCNGG 3 cut(s) 109, 315, 628
SduI GDGCHC 1 cut(s) 116
SfoI GGCGCC 2 cut(s) 394, 607
Sfr303I CCGCGG 1 cut(s) 391
SfuI TTCGAA 1 cut(s) 695
SgrBI CCGCGG 1 cut(s) 391
Sse9I AATT 1 cut(s) 21
SsiI CCGC 9 cut(s) 103, 171, 225, 373, 388, 390, 495, 603, 609
SspDI GGCGCC 2 cut(s) 392, 605
SspMI CTAG 1 cut(s) 600
StyD4I CCNGG 3 cut(s) 107, 313, 626
StyI CCWWGG 1 cut(s) 498
TaaI ACNGT 1 cut(s) 73
TaiI ACGT 4 cut(s) 324, 381, 453, 486
TaqI TCGA 3 cut(s) 258, 542, 695
TasI AATT 1 cut(s) 21
TauI GCSGC 6 cut(s) 227, 375, 393, 497, 606, 611
TfiI GAWTC 2 cut(s) 622, 697
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 2 cut(s) 10, 141
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 1 cut(s) 319
TspGWI ACGGA 2 cut(s) 241, 513
XagI CCTNNNNNAGG 1 cut(s) 425
XmiI GTMKAC 1 cut(s) 75
XspI CTAG 1 cut(s) 600
ZraI GACGTC 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.