AT5G15390

tRNA rRNA methyltransferase (SpoU) family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005805
Physical Location & Seq
Forward (+)
4995138 .. 4997489
2352 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G15390.1

Sequence Viewer

Length: 1053 bp
ATGAACTCGTGCAAATCCCTTATCCGTGCCTCAATCTCTTCCTTTCCATTTGTTCCTCTTCCAAACCCTAATTTCTCTATCACCTTCATCTCCGTTCGCGCCTTTTCTCCTCTCTCTGTCCTTCATCCAAATTCATCATGTATTGTAACAGCTCGACGCACATTTCATGGAGCTGTGGCTCTTTCACCGGAGAGCTTAACGGAAGAGTCTCCCAAGGATACTGTGAAAGGATTGTTAACAACCAATCGTGGTGAGGCCTCAAGCTTGATGAAGATGGAGCGGAGGTGTAGCTTAAGCAATGGTGAGGGTGATTGTAGAGGATCTTGGTTTCCTTACGAGGATAGGTTCAGGTGTGGTGAGGTGCATCTTAGTAGCCGTGAGGTGTTGGAGGCGGTGAGTCCTCATATGATGGAAGAGAGGACAGATAGGTTCAGGCGTGTGGTGGAGAATCGAAGCTACTCGGTGTGTCTTGTGGTTGAAGGTCTCTCTGATTTTGGAAACATCTCTGCTGCTTTCCGCTCAGCTGATGCGTTGGGGATTCAATCTGTACATGTTGTTTCCTGTGACAGCTCCAAAAGGTATAACGGAAATCGCCATGTGAGTATGGGAGCTGAGAAATGGCTGGACATTGAGTTTTGGGACACACCTAAAGAATGTTTCAAAGTTTTGAAGTCTCGCGGTTACAGAATAGCTACAACTCATTTGGGAATGGACACGGTTTCCATTTACGATATGGACTGGTCATGCCCAACAGCAATAGTTGTTGGAAACGAGGGCAGGGGAATAAGTGACGAAGCGTTGGAGTTATCAGATCTGCGTTGCAGTATTCCGATGAATGGAATGGTTGATTCGTTTAATGTCTCAGTGGCTGCAGGGATTCTGATGCACCACGCAGTCTCTGATAGAACCACTCGTCTGGGAAGCCATGGAGATCTGTCGGAAGCAGAGAAAGAGATATTGATGGCTGAGTTCTCACTTCGTCACAGTAGAAGCTCAATTTGCATTGCTTACGAGTTCGCTAAACGTAAACAACAACACTCTACCTCCTCCTAA

Protein Analysis

350

Amino Acids

38.85

Weight (kDa)

6.64

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 155 - 296 1.2e-36 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 280, 519
AccII CGCG 2 cut(s) 99, 678
AciI CCGC 4 cut(s) 280, 392, 517, 678
AclWI GGATC 1 cut(s) 328
AcsI RAATTY 1 cut(s) 130
AfaI GTAC 1 cut(s) 549
AfiI CCNNNNNNNGG 1 cut(s) 836
AflII CTTAAG 1 cut(s) 292
AflIII ACRYGT 1 cut(s) 550
AgsI TTSAA 4 cut(s) 479, 542, 661, 670
Alw26I GTCTC 5 cut(s) 213, 488, 678, 865, 901
AlwI GGATC 1 cut(s) 328
AlwNI CAGNNNCTG 2 cut(s) 869, 899
AoxI GGCC 1 cut(s) 255
ApeKI GCWGC 2 cut(s) 509, 869
ApoI RAATTY 1 cut(s) 130
AspLEI GCGC 1 cut(s) 101
AsuHPI GGTGA 7 cut(s) 73, 177, 263, 314, 320, 368, 406
BauI CACGAG 1 cut(s) 7
BbvI GCAGC 2 cut(s) 496, 856
BccI CCATC 3 cut(s) 268, 403, 955
BceAI ACGGC 1 cut(s) 360
BciVI GTATCC 1 cut(s) 211
BcoDI GTCTC 5 cut(s) 213, 488, 678, 865, 901
BfmI CTRYAG 1 cut(s) 870
BfrI CTTAAG 1 cut(s) 292
BfuI GTATCC 1 cut(s) 211
BglII AGATCT 2 cut(s) 811, 931
BisI GCNGC 2 cut(s) 510, 870
BlpI GCTNAGC 1 cut(s) 520
BlsI GCNGC 2 cut(s) 511, 871
BmsI GCATC 3 cut(s) 373, 517, 873
Bpu1102I GCTNAGC 1 cut(s) 520
BpuEI CTTGAG 1 cut(s) 244
BsaI GGTCTC 1 cut(s) 488
BsaJI CCNNGG 2 cut(s) 213, 925
BsaWI WCCGGW 1 cut(s) 187
BsaXI ACNNNNNCTCC 3 cut(s) 269, 299, 1028
Bsc4I CCNNNNNNNGG 1 cut(s) 836
Bse1I ACTGG 1 cut(s) 743
Bse3DI GCAATG 2 cut(s) 304, 1002
BseDI CCNNGG 2 cut(s) 213, 925
BseGI GGATG 1 cut(s) 124
BseLI CCNNNNNNNGG 1 cut(s) 836
BseMI GCAATG 2 cut(s) 304, 1002
BseMII CTCAG 4 cut(s) 534, 603, 876, 957
BseNI ACTGG 1 cut(s) 743
BseRI GAGGAG 2 cut(s) 99, 1036
BseXI GCAGC 2 cut(s) 496, 856
Bsh1236I CGCG 2 cut(s) 99, 678
BshFI GGCC 1 cut(s) 257
BsiSI CCGG 1 cut(s) 188
BslFI GGGAC 1 cut(s) 653
BslI CCNNNNNNNGG 1 cut(s) 836
BsmAI GTCTC 5 cut(s) 213, 488, 678, 865, 901
BsmFI GGGAC 1 cut(s) 653
BsnI GGCC 1 cut(s) 257
Bso31I GGTCTC 1 cut(s) 488
Bsp1407I TGTACA 1 cut(s) 547
Bsp143I GATC 3 cut(s) 320, 811, 931
Bsp1720I GCTNAGC 1 cut(s) 520
Bsp19I CCATGG 1 cut(s) 925
BspACI CCGC 4 cut(s) 280, 392, 517, 678
BspANI GGCC 1 cut(s) 257
BspCNI CTCAG 4 cut(s) 533, 604, 875, 958
BspFNI CGCG 2 cut(s) 99, 678
BspMAI CTGCAG 1 cut(s) 874
BspPI GGATC 1 cut(s) 328
BspTI CTTAAG 1 cut(s) 292
BspTNI GGTCTC 1 cut(s) 488
BsrBI CCGCTC 2 cut(s) 280, 519
BsrDI GCAATG 2 cut(s) 304, 1002
BsrGI TGTACA 1 cut(s) 547
BsrI ACTGG 1 cut(s) 743
BssECI CCNNGG 2 cut(s) 213, 925
BssMI GATC 3 cut(s) 320, 811, 931
BssSI CACGAG 1 cut(s) 7
BssT1I CCWWGG 2 cut(s) 213, 925
Bst2BI CACGAG 1 cut(s) 7
Bst4CI ACNGT 3 cut(s) 223, 718, 986
Bst6I CTCTTC 4 cut(s) 43, 63, 198, 408
BstAFI CTTAAG 1 cut(s) 292
BstAUI TGTACA 1 cut(s) 547
BstDEI CTNAG 5 cut(s) 368, 520, 612, 862, 966
BstDSI CCRYGG 1 cut(s) 925
BstF5I GGATG 1 cut(s) 124
BstFNI CGCG 2 cut(s) 99, 678
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 3 cut(s) 323, 814, 934
BstMAI GTCTC 5 cut(s) 213, 488, 678, 865, 901
BstMBI GATC 3 cut(s) 320, 811, 931
BstMWI GCNNNNNNNGC 1 cut(s) 999
BstNSI RCATGY 1 cut(s) 554
BstSFI CTRYAG 1 cut(s) 870
BstUI CGCG 2 cut(s) 99, 678
BstV1I GCAGC 2 cut(s) 496, 856
BstX2I RGATCY 3 cut(s) 320, 811, 931
BstXI CCANNNNNNTGG 1 cut(s) 916
BstYI RGATCY 3 cut(s) 320, 811, 931
BsuI GTATCC 1 cut(s) 211
BsuRI GGCC 1 cut(s) 257
BtgI CCRYGG 1 cut(s) 925
BtsCI GGATG 1 cut(s) 124
BtsIMutI CAGTG 1 cut(s) 870
CaiI CAGNNNCTG 2 cut(s) 869, 899
CfoI GCGC 1 cut(s) 101
CseI GACGC 1 cut(s) 165
Csp6I GTAC 1 cut(s) 548
CviAII CATG 6 cut(s) 138, 167, 551, 596, 744, 926
CviQI GTAC 1 cut(s) 548
DdeI CTNAG 5 cut(s) 368, 520, 612, 862, 966
DpnI GATC 3 cut(s) 322, 813, 933
DpnII GATC 3 cut(s) 320, 811, 931
Eam1104I CTCTTC 4 cut(s) 43, 63, 198, 408
EarI CTCTTC 4 cut(s) 43, 63, 198, 408
Eco130I CCWWGG 2 cut(s) 213, 925
Eco147I AGGCCT 1 cut(s) 257
Eco31I GGTCTC 1 cut(s) 488
EcoT14I CCWWGG 2 cut(s) 213, 925
ErhI CCWWGG 2 cut(s) 213, 925
FaeI CATG 6 cut(s) 141, 170, 554, 599, 747, 929
FaqI GGGAC 1 cut(s) 653
FatI CATG 6 cut(s) 137, 166, 550, 595, 743, 925
FauNDI CATATG 1 cut(s) 405
Fnu4HI GCNGC 2 cut(s) 510, 870
FokI GGATG 1 cut(s) 111
Fsp4HI GCNGC 2 cut(s) 510, 870
GlaI GCGC 1 cut(s) 100
GluI GCNGC 2 cut(s) 510, 870
HaeIII GGCC 1 cut(s) 257
HapII CCGG 1 cut(s) 188
HgaI GACGC 1 cut(s) 165
HhaI GCGC 1 cut(s) 101
Hin1II CATG 6 cut(s) 141, 170, 554, 599, 747, 929
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HincII GTYRAC 1 cut(s) 237
HindII GTYRAC 1 cut(s) 237
HindIII AAGCTT 1 cut(s) 262
HinfI GANTC 6 cut(s) 206, 397, 448, 538, 848, 877
HpaI GTTAAC 1 cut(s) 237
HpaII CCGG 1 cut(s) 188
HphI GGTGA 7 cut(s) 73, 177, 263, 314, 320, 368, 406
Hpy166II GTNNAC 2 cut(s) 237, 1028
Hpy188I TCNGA 6 cut(s) 490, 811, 831, 882, 901, 940
Hpy8I GTNNAC 2 cut(s) 237, 1028
Hpy99I CGWCG 1 cut(s) 159
HpyAV CCTTC 3 cut(s) 94, 131, 473
HpyCH4III ACNGT 3 cut(s) 223, 718, 986
HpyCH4IV ACGT 1 cut(s) 1024
HpyCH4V TGCA 6 cut(s) 12, 364, 822, 872, 886, 1002
HpyF10VI GCNNNNNNNGC 1 cut(s) 999
HpyF3I CTNAG 5 cut(s) 368, 520, 612, 862, 966
HpySE526I ACGT 1 cut(s) 1024
Hsp92II CATG 6 cut(s) 141, 170, 554, 599, 747, 929
HspAI GCGC 1 cut(s) 99
KspAI GTTAAC 1 cut(s) 237
Kzo9I GATC 3 cut(s) 320, 811, 931
LmnI GCTCC 4 cut(s) 170, 277, 575, 608
LpnPI CCDG 9 cut(s) 201, 334, 418, 574, 608, 724, 763, 858, 902
Lsp1109I GCAGC 2 cut(s) 496, 856
LweI GCATC 3 cut(s) 373, 517, 873
MaeII ACGT 1 cut(s) 1024
MaeIII GTNAC 5 cut(s) 145, 563, 680, 788, 980
MalI GATC 3 cut(s) 322, 813, 933
MbiI CCGCTC 2 cut(s) 280, 519
MboI GATC 3 cut(s) 320, 811, 931
MboII GAAGA 5 cut(s) 30, 50, 215, 283, 425
MflI RGATCY 3 cut(s) 320, 811, 931
MluCI AATT 3 cut(s) 70, 130, 996
MlyI GAGTC 2 cut(s) 215, 406
MmeI TCCRAC 4 cut(s) 366, 745, 780, 918
MseI TTAA 4 cut(s) 197, 236, 293, 855
MspA1I CMGCKG 1 cut(s) 524
MspCI CTTAAG 1 cut(s) 292
MspI CCGG 1 cut(s) 188
MvnI CGCG 2 cut(s) 99, 678
MwoI GCNNNNNNNGC 1 cut(s) 999
NcoI CCATGG 1 cut(s) 925
NdeI CATATG 1 cut(s) 405
NdeII GATC 3 cut(s) 320, 811, 931
NlaIII CATG 6 cut(s) 141, 170, 554, 599, 747, 929
NmuCI GTSAC 3 cut(s) 563, 788, 980
NspI RCATGY 1 cut(s) 554
PceI AGGCCT 1 cut(s) 257
PciI ACATGT 1 cut(s) 550
PfeI GAWTC 4 cut(s) 448, 538, 848, 877
PkrI GCNGC 2 cut(s) 511, 871
PleI GAGTC 2 cut(s) 214, 405
PpsI GAGTC 2 cut(s) 214, 405
PscI ACATGT 1 cut(s) 550
PstI CTGCAG 1 cut(s) 874
PstNI CAGNNNCTG 2 cut(s) 869, 899
PsuI RGATCY 3 cut(s) 320, 811, 931
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 1 cut(s) 549
RsaNI GTAC 1 cut(s) 548
SaqAI TTAA 4 cut(s) 197, 236, 293, 855
SatI GCNGC 2 cut(s) 510, 870
Sau3AI GATC 3 cut(s) 320, 811, 931
SchI GAGTC 2 cut(s) 215, 406
SfaNI GCATC 3 cut(s) 373, 517, 873
SfcI CTRYAG 1 cut(s) 870
SmlI CTYRAG 2 cut(s) 259, 292
SmoI CTYRAG 2 cut(s) 259, 292
Sse9I AATT 3 cut(s) 70, 130, 996
SseBI AGGCCT 1 cut(s) 257
SsiI CCGC 4 cut(s) 280, 392, 517, 678
StuI AGGCCT 1 cut(s) 257
StyI CCWWGG 2 cut(s) 213, 925
TaaI ACNGT 3 cut(s) 223, 718, 986
TaiI ACGT 1 cut(s) 1027
TaqI TCGA 2 cut(s) 154, 451
TasI AATT 3 cut(s) 70, 130, 996
TatI WGTACW 1 cut(s) 547
TfiI GAWTC 4 cut(s) 448, 538, 848, 877
Tru1I TTAA 4 cut(s) 197, 236, 293, 855
Tru9I TTAA 4 cut(s) 197, 236, 293, 855
TscAI CASTG 1 cut(s) 870
TseFI GTSAC 3 cut(s) 563, 788, 980
TseI GCWGC 2 cut(s) 509, 869
Tsp45I GTSAC 3 cut(s) 563, 788, 980
TspDTI ATGAA 7 cut(s) 17, 76, 113, 123, 155, 284, 848
TspGWI ACGGA 4 cut(s) 14, 82, 215, 600
TspRI CASTG 1 cut(s) 870
Vha464I CTTAAG 1 cut(s) 292
XapI RAATTY 1 cut(s) 130
XceI RCATGY 1 cut(s) 554
XcmI CCANNNNNNNNNTGG 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.