Rh4DG034600

tRNA nucleoside ribose methylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005805
Physical Location & Seq
Forward (+)
5868693 .. 5872442
3750 bp
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UTR
Exon/CDS
Intron
Rh4DG034600.1

Sequence Viewer

Length: 273 bp
ATGGCCGAATTCGCAGAGAGAAGACTTAAACCTTCATCTCCAACAAACGCAAGCATAAGGGGAATAAGTGATGAGGCCCTTGATTTGTCAGATTTGCATTGTAGTATTCCAATGAAGGGCATGGTGGATTCTTTCAATGTTTCAGTTGCTGCTGGCATTCTCATGCACCATGCTGTTTGTGATAGAACTTCTCGCCTGGGCTGTCATGGTGATCTGACTCTTAACTCCGGATCAGGTTTTTGGATGGAAGACTGGACTTTTATAAAAAGATGA

Protein Analysis

90

Amino Acids

9.9

Weight (kDa)

6.02

Isoelectric Point (pI)

40.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 20 - 56 2e-09 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 263
AccIII TCCGGA 1 cut(s) 227
AclWI GGATC 1 cut(s) 238
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 8
AfiI CCNNNNNNNGG 1 cut(s) 116
AgsI TTSAA 1 cut(s) 136
AjnI CCWGG 1 cut(s) 195
AlwI GGATC 1 cut(s) 238
AlwNI CAGNNNCTG 1 cut(s) 149
Aor13HI TCCGGA 1 cut(s) 227
AoxI GGCC 2 cut(s) 3, 75
ApeKI GCWGC 1 cut(s) 149
ApoI RAATTY 1 cut(s) 8
AspS9I GGNCC 1 cut(s) 76
AsuHPI GGTGA 1 cut(s) 221
BbsI GAAGAC 2 cut(s) 28, 255
BbvI GCAGC 1 cut(s) 136
BccI CCATC 1 cut(s) 238
BciT130I CCWGG 1 cut(s) 197
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
Bme1390I CCNGG 1 cut(s) 197
BmgT120I GGNCC 1 cut(s) 76
BmrFI CCNGG 1 cut(s) 197
BpiI GAAGAC 2 cut(s) 28, 255
BsaJI CCNNGG 1 cut(s) 196
BsaWI WCCGGW 1 cut(s) 227
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 257
BseAI TCCGGA 1 cut(s) 227
BseBI CCWGG 1 cut(s) 197
BseDI CCNNGG 1 cut(s) 196
BseGI GGATG 1 cut(s) 249
BseLI CCNNNNNNNGG 1 cut(s) 116
BseNI ACTGG 1 cut(s) 257
BseXI GCAGC 1 cut(s) 136
BshFI GGCC 2 cut(s) 5, 77
BsiSI CCGG 1 cut(s) 228
BslI CCNNNNNNNGG 1 cut(s) 116
BsmI GAATGC 1 cut(s) 156
BsnI GGCC 2 cut(s) 5, 77
Bsp13I TCCGGA 1 cut(s) 227
Bsp143I GATC 2 cut(s) 211, 230
BspANI GGCC 2 cut(s) 5, 77
BspEI TCCGGA 1 cut(s) 227
BspPI GGATC 1 cut(s) 238
BsrI ACTGG 1 cut(s) 257
BssECI CCNNGG 1 cut(s) 196
BssMI GATC 2 cut(s) 211, 230
Bst2UI CCWGG 1 cut(s) 197
BstC8I GCNNGC 2 cut(s) 52, 154
BstF5I GGATG 1 cut(s) 249
BstKTI GATC 2 cut(s) 214, 233
BstMBI GATC 2 cut(s) 211, 230
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 197
BstSCI CCNGG 1 cut(s) 195
BstV1I GCAGC 1 cut(s) 136
BstV2I GAAGAC 2 cut(s) 28, 255
BsuRI GGCC 2 cut(s) 5, 77
BtsCI GGATG 1 cut(s) 249
Cac8I GCNNGC 2 cut(s) 52, 154
CaiI CAGNNNCTG 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 76
CviAII CATG 4 cut(s) 121, 163, 170, 206
CviJI RGCY 3 cut(s) 5, 77, 201
CviKI_1 RGCY 3 cut(s) 5, 77, 201
DpnI GATC 2 cut(s) 213, 232
DpnII GATC 2 cut(s) 211, 230
EaeI YGGCCR 1 cut(s) 3
EcoO109I RGGNCCY 1 cut(s) 76
EcoRI GAATTC 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 195
FaeI CATG 4 cut(s) 124, 166, 173, 209
FaiI YATR 6 cut(s) 56, 122, 164, 171, 207, 263
FatI CATG 4 cut(s) 120, 162, 169, 205
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 1 cut(s) 256
Fsp4HI GCNGC 1 cut(s) 150
GluI GCNGC 1 cut(s) 150
HaeIII GGCC 2 cut(s) 5, 77
HapII CCGG 1 cut(s) 228
Hin1II CATG 4 cut(s) 124, 166, 173, 209
HinfI GANTC 2 cut(s) 128, 217
HpaII CCGG 1 cut(s) 228
HphI GGTGA 1 cut(s) 221
Hpy188I TCNGA 2 cut(s) 91, 216
Hpy188III TCNNGA 1 cut(s) 228
HpyAV CCTTC 2 cut(s) 42, 109
HpyCH4V TGCA 2 cut(s) 97, 166
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
Hsp92II CATG 4 cut(s) 124, 166, 173, 209
Kpn2I TCCGGA 1 cut(s) 227
Kzo9I GATC 2 cut(s) 211, 230
LpnPI CCDG 6 cut(s) 138, 182, 209, 219, 238, 241
Lsp1109I GCAGC 1 cut(s) 136
MalI GATC 2 cut(s) 213, 232
MboI GATC 2 cut(s) 211, 230
MboII GAAGA 2 cut(s) 33, 260
MluCI AATT 1 cut(s) 8
MlyI GAGTC 1 cut(s) 211
MmeI TCCRAC 1 cut(s) 65
MnlI CCTC 1 cut(s) 67
MroI TCCGGA 1 cut(s) 227
MseI TTAA 2 cut(s) 27, 222
MslI CAYNNNNRTG 1 cut(s) 161
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 1 cut(s) 197
Mva1269I GAATGC 1 cut(s) 156
MvaI CCWGG 1 cut(s) 197
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeII GATC 2 cut(s) 211, 230
NlaIII CATG 4 cut(s) 124, 166, 173, 209
PctI GAATGC 1 cut(s) 156
PfeI GAWTC 1 cut(s) 128
PkrI GCNGC 1 cut(s) 151
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
PsiI TTATAA 1 cut(s) 263
Psp6I CCWGG 1 cut(s) 195
PspGI CCWGG 1 cut(s) 195
PspPI GGNCC 1 cut(s) 76
PstNI CAGNNNCTG 1 cut(s) 149
RseI CAYNNNNRTG 1 cut(s) 161
SaqAI TTAA 2 cut(s) 27, 222
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 2 cut(s) 211, 230
Sau96I GGNCC 1 cut(s) 76
SchI GAGTC 1 cut(s) 211
ScrFI CCNGG 1 cut(s) 197
SetI ASST 2 cut(s) 34, 238
SmiMI CAYNNNNRTG 1 cut(s) 161
Sse9I AATT 1 cut(s) 8
StyD4I CCNGG 1 cut(s) 195
TasI AATT 1 cut(s) 8
TfiI GAWTC 1 cut(s) 128
Tru1I TTAA 2 cut(s) 27, 222
Tru9I TTAA 2 cut(s) 27, 222
TseI GCWGC 1 cut(s) 149
TspDTI ATGAA 2 cut(s) 24, 128
XapI RAATTY 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.