FvH4_6g47560

tRNA nucleoside ribose methylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005805
Physical Location & Seq
Reverse (-)
36007948 .. 36010853
2906 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g47560.t5

Sequence Viewer

Length: 1065 bp
ATGGCGGCCTGCAGGACCCTAATCCACACTTCTCTCTCTTCAATCGGCTCCACTCTCCGGCCCCAATTCACCCCTCTTCTCAATCTCTGCTTCTCTCCTCTCCTTTCCCCCAATAAAGGGCTCAGACTCTCCCAGAACAACCTCCCTCATCGGCCGCACGCTTCAATTGCAGCAGCAGAAGCCGCAGATTTCGAAGCTCCGTCCCCAATTTCCGAAACCCTAGATCCGCCGGAGTCACAAGACACGGTGGAGCACCTCCTCACGCACCGGGACGACGTCGTGAAGCTCATGAAAATGGAACGCCGCCGGAGCGGCTGCGGCGGCGGCGGGCGGTGGTTTCCGTACCTGGACAGGTTCAACTGCGGCGGTGCTTACGTTACGAGTGGTGAGATACTGGAGGCGGTGGATCCGATTATAATGGATGTGAGGAAGGAGAGGTTTCGGAATGTGGTGAGGAACCGGAGCTACTCGGTGTGCTTGGTGGTGGAGGGGTTGGGAGATTTTGGGAATGTCTCTGCGGTGTTCCGGTCCGCCGACGCGCTTGGGATTCAGTCGGTTCATGTGCTCTCTTGTGACGCTTCGAAAAGGTACAGAGAAAATCGACATGTGAGCATGGGAGCTGAGAAATGGTTGGATATTGAACTTTGGGACTCCACTCAAGAATGCTTTGAAGTTCTAACATCGCGTGGTTATCGAATTGCCACAACACATGTGGGGATGGATGCGGTGTCTATTTACAACATGGACTGGTCTTGCCCAACTGCAATAGTTGTCGGAAATGAAAATAGGGGAATAAGTGATGAGGCCCTGGATTTGTCAGATTTGCATTGCAGTATTCCAATGAAAGGGATGGTGGATTCTTTCAATGTTTCAGTTGCTGCTGGCATTCTCATGCACCATGCTGTTTGTGATAGAACTTCTCGCCTGGGCAGTCATGGTGATCTGACACCTGAAGAGAACCAAATTCTTCTTGCTGAGTTCTCTCTGCGTCACAGCAAGAATTCAATTAGCATTGCTCACGAGTACGCAAAGCGGAAGGCAGCTTTGCTCACACCAAGGCTTTGA

Protein Analysis

355

Amino Acids

39.09

Weight (kDa)

6.27

Isoelectric Point (pI)

47.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 158 - 263 6.4e-19 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 416
AatII GACGTC 1 cut(s) 279
AccBSI CCGCTC 1 cut(s) 312
AccII CGCG 2 cut(s) 539, 685
AclWI GGATC 3 cut(s) 218, 401, 414
AcoI YGGCCR 1 cut(s) 152
AcsI RAATTY 2 cut(s) 963, 1000
AcuI CTGAAG 1 cut(s) 972
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 3 cut(s) 344, 590, 1025
AfiI CCNNNNNNNGG 4 cut(s) 57, 116, 117, 845
AflIII ACRYGT 2 cut(s) 604, 709
AgsI TTSAA 7 cut(s) 42, 165, 358, 641, 671, 865, 1005
AjnI CCWGG 3 cut(s) 345, 807, 924
AluBI AGCT 5 cut(s) 197, 286, 465, 620, 1043
AluI AGCT 5 cut(s) 197, 286, 465, 620, 1043
Alw21I GWGCWC 2 cut(s) 255, 567
Alw26I GTCTC 1 cut(s) 517
AlwI GGATC 3 cut(s) 218, 401, 414
AlwNI CAGNNNCTG 1 cut(s) 878
AoxI GGCC 4 cut(s) 6, 59, 152, 804
ApeKI GCWGC 5 cut(s) 170, 173, 315, 878, 1040
ApoI RAATTY 2 cut(s) 963, 1000
AspLEI GCGC 1 cut(s) 541
AspS9I GGNCC 4 cut(s) 15, 60, 528, 805
AsuC2I CCSGG 1 cut(s) 269
AsuHPI GGTGA 4 cut(s) 61, 398, 463, 950
AsuII TTCGAA 2 cut(s) 192, 581
AvaII GGWCC 2 cut(s) 15, 528
BamHI GGATCC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 123
BauI CACGAG 1 cut(s) 1019
Bbv12I GWGCWC 2 cut(s) 255, 567
BbvI GCAGC 5 cut(s) 182, 185, 302, 865, 1052
BccI CCATC 2 cut(s) 712, 844
BciT130I CCWGG 3 cut(s) 347, 809, 926
BcnI CCSGG 1 cut(s) 269
BcoDI GTCTC 1 cut(s) 517
BfaI CTAG 1 cut(s) 221
BfmI CTRYAG 1 cut(s) 10
BglI GCCNNNNNGGC 1 cut(s) 312
Bme1390I CCNGG 4 cut(s) 269, 347, 809, 926
Bme18I GGWCC 2 cut(s) 15, 528
BmgT120I GGNCC 4 cut(s) 15, 60, 528, 805
BmiI GGNNCC 5 cut(s) 17, 49, 62, 408, 458
BmrFI CCNGG 4 cut(s) 269, 347, 809, 926
BmsI GCATC 1 cut(s) 712
BpmI CTGGAG 1 cut(s) 416
Bpu14I TTCGAA 2 cut(s) 192, 581
BpuEI CTTGAG 1 cut(s) 642
BpuMI CCSGG 1 cut(s) 269
BsaHI GRCGYC 1 cut(s) 276
BsaJI CCNNGG 3 cut(s) 807, 925, 1055
BsaWI WCCGGW 2 cut(s) 459, 525
Bsc4I CCNNNNNNNGG 4 cut(s) 57, 116, 117, 845
Bse1I ACTGG 2 cut(s) 399, 752
Bse3DI GCAATG 2 cut(s) 826, 1011
BseBI CCWGG 3 cut(s) 347, 809, 926
BseDI CCNNGG 3 cut(s) 807, 925, 1055
BseGI GGATG 4 cut(s) 427, 723, 727, 855
BseLI CCNNNNNNNGG 4 cut(s) 57, 116, 117, 845
BseMI GCAATG 2 cut(s) 826, 1011
BseMII CTCAG 3 cut(s) 136, 612, 966
BseNI ACTGG 2 cut(s) 399, 752
BseRI GAGGAG 2 cut(s) 87, 248
BseX3I CGGCCG 1 cut(s) 152
BseXI GCAGC 5 cut(s) 182, 185, 302, 865, 1052
Bsh1236I CGCG 2 cut(s) 539, 685
Bsh1285I CGRYCG 1 cut(s) 155
BshFI GGCC 4 cut(s) 8, 61, 154, 806
BsiEI CGRYCG 1 cut(s) 155
BsiHKAI GWGCWC 2 cut(s) 255, 567
BsiSI CCGG 6 cut(s) 58, 230, 268, 307, 460, 526
BslFI GGGAC 3 cut(s) 187, 284, 662
BslI CCNNNNNNNGG 4 cut(s) 57, 116, 117, 845
BsmAI GTCTC 1 cut(s) 517
BsmFI GGGAC 3 cut(s) 187, 284, 662
BsmI GAATGC 2 cut(s) 668, 885
BsnI GGCC 4 cut(s) 8, 61, 154, 806
Bsp119I TTCGAA 2 cut(s) 192, 581
Bsp1286I GDGCHC 3 cut(s) 123, 255, 567
Bsp143I GATC 3 cut(s) 223, 406, 940
BspANI GGCC 4 cut(s) 8, 61, 154, 806
BspCNI CTCAG 3 cut(s) 135, 613, 967
BspFNI CGCG 2 cut(s) 539, 685
BspHI TCATGA 1 cut(s) 288
BspLI GGNNCC 5 cut(s) 17, 49, 62, 408, 458
BspMAI CTGCAG 1 cut(s) 14
BspPI GGATC 3 cut(s) 218, 401, 414
BspT104I TTCGAA 2 cut(s) 192, 581
BsrBI CCGCTC 1 cut(s) 312
BsrDI GCAATG 2 cut(s) 826, 1011
BsrI ACTGG 2 cut(s) 399, 752
BssECI CCNNGG 3 cut(s) 807, 925, 1055
BssMI GATC 3 cut(s) 223, 406, 940
BssNI GRCGYC 1 cut(s) 276
BssSI CACGAG 1 cut(s) 1019
BssT1I CCWWGG 1 cut(s) 1055
Bst2BI CACGAG 1 cut(s) 1019
Bst2UI CCWGG 3 cut(s) 347, 809, 926
Bst4CI ACNGT 1 cut(s) 247
Bst6I CTCTTC 3 cut(s) 43, 81, 948
BstACI GRCGYC 1 cut(s) 276
BstBI TTCGAA 2 cut(s) 192, 581
BstC8I GCNNGC 4 cut(s) 10, 159, 329, 883
BstDEI CTNAG 3 cut(s) 122, 621, 975
BstF5I GGATG 4 cut(s) 427, 723, 727, 855
BstFNI CGCG 2 cut(s) 539, 685
BstHHI GCGC 1 cut(s) 541
BstKTI GATC 3 cut(s) 226, 409, 943
BstMAI GTCTC 1 cut(s) 517
BstMBI GATC 3 cut(s) 223, 406, 940
BstMCI CGRYCG 1 cut(s) 155
BstMWI GCNNNNNNNGC 8 cut(s) 167, 179, 182, 309, 312, 318, 321, 324
BstNI CCWGG 3 cut(s) 347, 809, 926
BstNSI RCATGY 2 cut(s) 608, 713
BstSCI CCNGG 4 cut(s) 267, 345, 807, 924
BstSFI CTRYAG 1 cut(s) 10
BstUI CGCG 2 cut(s) 539, 685
BstV1I GCAGC 5 cut(s) 182, 185, 302, 865, 1052
BstX2I RGATCY 2 cut(s) 223, 406
BstYI RGATCY 2 cut(s) 223, 406
BstZI CGGCCG 1 cut(s) 152
BsuRI GGCC 4 cut(s) 8, 61, 154, 806
BtgZI GCGATG 1 cut(s) 666
BtsCI GGATG 4 cut(s) 427, 723, 727, 855
Cac8I GCNNGC 4 cut(s) 10, 159, 329, 883
CaiI CAGNNNCTG 1 cut(s) 878
CciI TCATGA 1 cut(s) 288
CfoI GCGC 1 cut(s) 541
Cfr13I GGNCC 4 cut(s) 15, 60, 528, 805
CpoI CGGWCCG 1 cut(s) 528
CseI GACGC 3 cut(s) 545, 584, 977
Csp6I GTAC 3 cut(s) 343, 589, 1024
CspCI CAANNNNNGTGG 2 cut(s) 693, 728
CspI CGGWCCG 1 cut(s) 528
CviAII CATG 9 cut(s) 289, 560, 605, 613, 710, 742, 892, 899, 935
CviQI GTAC 3 cut(s) 343, 589, 1024
DdeI CTNAG 3 cut(s) 122, 621, 975
DpnI GATC 3 cut(s) 225, 408, 942
DpnII GATC 3 cut(s) 223, 406, 940
EaeI YGGCCR 1 cut(s) 152
EagI CGGCCG 1 cut(s) 152
Eam1104I CTCTTC 3 cut(s) 43, 81, 948
EarI CTCTTC 3 cut(s) 43, 81, 948
EciI GGCGGA 2 cut(s) 216, 520
EclXI CGGCCG 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 1055
Eco24I GRGCYC 1 cut(s) 123
Eco47I GGWCC 2 cut(s) 15, 528
Eco52I CGGCCG 1 cut(s) 152
Eco57I CTGAAG 1 cut(s) 972
EcoO109I RGGNCCY 2 cut(s) 15, 805
EcoRI GAATTC 1 cut(s) 1000
EcoRII CCWGG 3 cut(s) 345, 807, 924
EcoT14I CCWWGG 1 cut(s) 1055
EcoT38I GRGCYC 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 1055
FaeI CATG 9 cut(s) 292, 563, 608, 616, 713, 745, 895, 902, 938
FaqI GGGAC 3 cut(s) 187, 284, 662
FatI CATG 9 cut(s) 288, 559, 604, 612, 709, 741, 891, 898, 934
FauI CCCGC 1 cut(s) 320
FokI GGATG 4 cut(s) 434, 730, 734, 862
FriOI GRGCYC 1 cut(s) 123
FspBI CTAG 1 cut(s) 221
GlaI GCGC 1 cut(s) 540
GsuI CTGGAG 1 cut(s) 416
HaeIII GGCC 4 cut(s) 8, 61, 154, 806
HapII CCGG 6 cut(s) 58, 230, 268, 307, 460, 526
HgaI GACGC 3 cut(s) 545, 584, 977
HhaI GCGC 1 cut(s) 541
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 9 cut(s) 292, 563, 608, 616, 713, 745, 895, 902, 938
Hin6I GCGC 1 cut(s) 539
HinP1I GCGC 1 cut(s) 539
HinfI GANTC 5 cut(s) 126, 233, 547, 650, 857
HpaII CCGG 6 cut(s) 58, 230, 268, 307, 460, 526
HphI GGTGA 4 cut(s) 61, 398, 463, 950
Hpy188I TCNGA 7 cut(s) 125, 214, 411, 444, 776, 820, 945
Hpy188III TCNNGA 4 cut(s) 280, 289, 659, 1019
Hpy99I CGWCG 3 cut(s) 278, 281, 539
HpyAV CCTTC 2 cut(s) 424, 1030
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 2 cut(s) 276, 375
HpyCH4V TGCA 6 cut(s) 12, 170, 764, 826, 831, 895
HpyF10VI GCNNNNNNNGC 8 cut(s) 167, 179, 182, 309, 312, 318, 321, 324
HpyF3I CTNAG 3 cut(s) 122, 621, 975
HpySE526I ACGT 2 cut(s) 276, 375
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 9 cut(s) 292, 563, 608, 616, 713, 745, 895, 902, 938
HspAI GCGC 1 cut(s) 539
Kzo9I GATC 3 cut(s) 223, 406, 940
LmnI GCTCC 6 cut(s) 53, 202, 250, 309, 462, 617
Lsp1109I GCAGC 5 cut(s) 182, 185, 302, 865, 1052
LweI GCATC 1 cut(s) 712
MaeI CTAG 1 cut(s) 221
MaeII ACGT 2 cut(s) 276, 375
MaeIII GTNAC 4 cut(s) 234, 376, 572, 989
MalI GATC 3 cut(s) 225, 408, 942
MbiI CCGCTC 1 cut(s) 312
MboI GATC 3 cut(s) 223, 406, 940
MboII GAAGA 4 cut(s) 30, 68, 959, 965
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 2 cut(s) 223, 406
MhlI GDGCHC 3 cut(s) 123, 255, 567
MluCI AATT 7 cut(s) 65, 165, 207, 696, 963, 1000, 1005
MlyI GAGTC 3 cut(s) 120, 242, 644
MmeI TCCRAC 2 cut(s) 612, 754
MslI CAYNNNNRTG 2 cut(s) 293, 890
MspI CCGG 6 cut(s) 58, 230, 268, 307, 460, 526
MspR9I CCNGG 4 cut(s) 269, 347, 809, 926
MunI CAATTG 1 cut(s) 165
Mva1269I GAATGC 2 cut(s) 668, 885
MvaI CCWGG 3 cut(s) 347, 809, 926
MvnI CGCG 2 cut(s) 539, 685
MwoI GCNNNNNNNGC 8 cut(s) 167, 179, 182, 309, 312, 318, 321, 324
NciI CCSGG 1 cut(s) 269
NdeII GATC 3 cut(s) 223, 406, 940
NlaIII CATG 9 cut(s) 292, 563, 608, 616, 713, 745, 895, 902, 938
NlaIV GGNNCC 5 cut(s) 17, 49, 62, 408, 458
NmuCI GTSAC 3 cut(s) 234, 572, 989
NspI RCATGY 2 cut(s) 608, 713
NspV TTCGAA 2 cut(s) 192, 581
PagI TCATGA 1 cut(s) 288
PciI ACATGT 2 cut(s) 604, 709
PctI GAATGC 2 cut(s) 668, 885
PfeI GAWTC 2 cut(s) 547, 857
PflFI GACNNNGTC 1 cut(s) 275
PleI GAGTC 3 cut(s) 120, 241, 644
PpsI GAGTC 3 cut(s) 120, 241, 644
PpuMI RGGWCCY 1 cut(s) 15
PscI ACATGT 2 cut(s) 604, 709
PsiI TTATAA 1 cut(s) 416
Psp5II RGGWCCY 1 cut(s) 15
Psp6I CCWGG 3 cut(s) 345, 807, 924
PspGI CCWGG 3 cut(s) 345, 807, 924
PspN4I GGNNCC 5 cut(s) 17, 49, 62, 408, 458
PspPI GGNCC 4 cut(s) 15, 60, 528, 805
PspPPI RGGWCCY 1 cut(s) 15
PsrI GAACNNNNNNTAC 2 cut(s) 449, 481
PstI CTGCAG 1 cut(s) 14
PstNI CAGNNNCTG 1 cut(s) 878
PsuI RGATCY 2 cut(s) 223, 406
PsyI GACNNNGTC 1 cut(s) 275
RsaI GTAC 3 cut(s) 344, 590, 1025
RsaNI GTAC 3 cut(s) 343, 589, 1024
RseI CAYNNNNRTG 2 cut(s) 293, 890
Rsr2I CGGWCCG 1 cut(s) 528
RsrII CGGWCCG 1 cut(s) 528
Sau3AI GATC 3 cut(s) 223, 406, 940
Sau96I GGNCC 4 cut(s) 15, 60, 528, 805
SbfI CCTGCAGG 1 cut(s) 14
SchI GAGTC 3 cut(s) 120, 242, 644
ScrFI CCNGG 4 cut(s) 269, 347, 809, 926
SdaI CCTGCAGG 1 cut(s) 14
SduI GDGCHC 3 cut(s) 123, 255, 567
SfaNI GCATC 1 cut(s) 712
SfcI CTRYAG 1 cut(s) 10
SfuI TTCGAA 2 cut(s) 192, 581
SinI GGWCC 2 cut(s) 15, 528
SmiMI CAYNNNNRTG 2 cut(s) 293, 890
SmlI CTYRAG 1 cut(s) 657
SmoI CTYRAG 1 cut(s) 657
Sse8387I CCTGCAGG 1 cut(s) 14
Sse9I AATT 7 cut(s) 65, 165, 207, 696, 963, 1000, 1005
SspMI CTAG 1 cut(s) 221
StyD4I CCNGG 4 cut(s) 267, 345, 807, 924
StyI CCWWGG 1 cut(s) 1055
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 2 cut(s) 279, 378
TaqI TCGA 4 cut(s) 192, 581, 601, 694
TasI AATT 7 cut(s) 65, 165, 207, 696, 963, 1000, 1005
TauI GCSGC 9 cut(s) 8, 157, 185, 306, 315, 321, 324, 327, 366
TfiI GAWTC 2 cut(s) 547, 857
TseFI GTSAC 3 cut(s) 234, 572, 989
TseI GCWGC 5 cut(s) 170, 173, 315, 878, 1040
Tsp45I GTSAC 3 cut(s) 234, 572, 989
TspDTI ATGAA 4 cut(s) 305, 548, 795, 857
TspGWI ACGGA 2 cut(s) 189, 330
Tth111I GACNNNGTC 1 cut(s) 275
VpaK11BI GGWCC 2 cut(s) 15, 528
XapI RAATTY 2 cut(s) 963, 1000
XceI RCATGY 2 cut(s) 608, 713
XcmI CCANNNNNNNNNTGG 1 cut(s) 709
XspI CTAG 1 cut(s) 221
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.