RLG00000021639

tRNA nucleoside ribose methylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005805
Physical Location & Seq
Reverse (-)
76929802 .. 76932296
2495 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021639

Sequence Viewer

Length: 1074 bp
ATGGCGACCTGCAAAACGCTAGTCCACACTTGTCTCTCTTCAATCGGTTCCACTCTCCGACCCCAATTCATCCCTCTCCTCAATCTCCGCTTCTCTCCTCTCCTCTCACCCAATAAAGGGCTCAGAGTTTCCCAGAACAATGCTCCTTCTCGGCCGTACGCTTCAATTGTAGCAGCAGAAGCCGCGGACTTCACAGACGAAGCTCCCTCCCCAAACGAAACCCTAGATCCATCTGAGTCACAAGACACCGTGGAGCACCTACTCACGCACCGAGACGACGTCGTAAAGTTCATGAAAATGGAGCGCCGCCCTCTCCGGTCCGCACGCGGCTGCGGCGCCCGGTGGTTCCCGTACCTCGACAGATTCAACTGCGGCGGTGCTTACCTCAGCAGCGGTGAGATACTGGAAGCGGTTGATCCGTACATTATGGATGTGAGGAAAGAGAGGTTTAGGAATGTGGTGAGGGGTCGGAGCTACTCGGTGTGCTTGGTGGTGGAGGGATTGGGAGATTTTGGAAATGTATCGGCAGTTTTCCGGTCCGCCGACGCTCTCGGGATTCAGTCGGTTCATGTGCTCTCTTGTGACGCGTCCAAAAGGTACAGAGAAAATCGTCATGTCAGCATGGGAGCAGAGAAATGGTTAGATATTGAACTTTGGGACTCCACTCAGGAGTGCTTTGAAGTTCTAACATCGCGTGGTTATCGAATTGCCACAACACACGTGGGGATGGATGCGGTGTCTATTTACGACATGGACTGGTCCTGCCCAACTGCAATAGTAGTCGGAAATGAAAATAGGGGAATAAGTGATGAGGCCCTGGATTTGTCAGATTTGCATTGCAGTATTCCAATGAAAGGCATGGTGGATTCTTTCAATGTTTCAGTTGCTGCAGGCATTCTCATGCACCATGCTGTTTGTGATAGAACTTCTCGCCTGGGCTGTCATGGTGATCTGACATCTGAAGAGAACCAAATTCTTCTTGCTGAGTTTTCTCTGCGTCATAGCAAGAATTCAATTAGCATTGCTCATGAGTATGCAAAGCGGAAGGCAGCTCTGCTCAAACCAAGGCTCTGA

Protein Analysis

358

Amino Acids

39.63

Weight (kDa)

6.32

Isoelectric Point (pI)

47.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 161 - 302 1.2e-35 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 282
Acc36I ACCTGC 1 cut(s) 17
AccB1I GGYRCC 1 cut(s) 335
AccII CGCG 4 cut(s) 185, 327, 587, 694
AclWI GGATC 2 cut(s) 221, 410
AcoI YGGCCR 1 cut(s) 152
AcsI RAATTY 2 cut(s) 972, 1009
AcuI CTGAAG 1 cut(s) 981
AcvI CACGTG 1 cut(s) 721
AcyI GRCGYC 2 cut(s) 279, 336
AfaI GTAC 4 cut(s) 158, 353, 422, 599
AfiI CCNNNNNNNGG 3 cut(s) 116, 117, 854
AflIII ACRYGT 2 cut(s) 585, 718
AgsI TTSAA 7 cut(s) 42, 165, 367, 650, 680, 874, 1014
AjnI CCWGG 2 cut(s) 816, 933
AluBI AGCT 3 cut(s) 203, 474, 1052
AluI AGCT 3 cut(s) 203, 474, 1052
Alw21I GWGCWC 2 cut(s) 258, 576
Alw26I GTCTC 2 cut(s) 38, 267
AlwI GGATC 2 cut(s) 221, 410
AlwNI CAGNNNCTG 1 cut(s) 887
Ama87I CYCGRG 1 cut(s) 551
AoxI GGCC 2 cut(s) 152, 813
ApeKI GCWGC 5 cut(s) 173, 330, 390, 887, 1049
ApoI RAATTY 2 cut(s) 972, 1009
ArsI GACNNNNNNTTYG 1 cut(s) 38
AspLEI GCGC 2 cut(s) 306, 338
AspS9I GGNCC 4 cut(s) 318, 537, 759, 814
AsuC2I CCSGG 1 cut(s) 340
AsuHPI GGTGA 4 cut(s) 99, 407, 472, 959
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 3 cut(s) 318, 537, 759
BanI GGYRCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 123
BbrPI CACGTG 1 cut(s) 721
Bbv12I GWGCWC 2 cut(s) 258, 576
BbvCI CCTCAGC 1 cut(s) 386
BbvI GCAGC 5 cut(s) 185, 317, 402, 874, 1061
BccI CCATC 2 cut(s) 238, 721
BceAI ACGGC 1 cut(s) 139
BciT130I CCWGG 2 cut(s) 818, 935
BcnI CCSGG 1 cut(s) 340
BcoDI GTCTC 2 cut(s) 38, 267
BfaI CTAG 2 cut(s) 20, 224
BfmI CTRYAG 1 cut(s) 888
BfoI RGCGCY 2 cut(s) 307, 339
BfuAI ACCTGC 1 cut(s) 17
Bme1390I CCNGG 3 cut(s) 340, 818, 935
Bme18I GGWCC 3 cut(s) 318, 537, 759
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 4 cut(s) 318, 537, 759, 814
BmiI GGNNCC 3 cut(s) 49, 337, 347
BmrFI CCNGG 3 cut(s) 340, 818, 935
BmsI GCATC 1 cut(s) 721
Bpu10I CCTNAGC 1 cut(s) 386
BpuMI CCSGG 1 cut(s) 340
BsaAI YACGTR 1 cut(s) 721
BsaHI GRCGYC 2 cut(s) 279, 336
BsaJI CCNNGG 5 cut(s) 183, 249, 816, 934, 1064
BsaWI WCCGGW 2 cut(s) 315, 534
Bsc4I CCNNNNNNNGG 3 cut(s) 116, 117, 854
Bse1I ACTGG 2 cut(s) 408, 761
Bse3DI GCAATG 2 cut(s) 835, 1020
BseBI CCWGG 2 cut(s) 818, 935
BseDI CCNNGG 5 cut(s) 183, 249, 816, 934, 1064
BseGI GGATG 4 cut(s) 69, 436, 732, 736
BseLI CCNNNNNNNGG 3 cut(s) 116, 117, 854
BseMI GCAATG 2 cut(s) 835, 1020
BseMII CTCAG 5 cut(s) 136, 225, 400, 680, 975
BseNI ACTGG 2 cut(s) 408, 761
BseRI GAGGAG 3 cut(s) 68, 87, 92
BseX3I CGGCCG 1 cut(s) 152
BseXI GCAGC 5 cut(s) 185, 317, 402, 874, 1061
Bsh1236I CGCG 4 cut(s) 185, 327, 587, 694
Bsh1285I CGRYCG 1 cut(s) 155
BshFI GGCC 2 cut(s) 154, 815
BshNI GGYRCC 1 cut(s) 335
BsiEI CGRYCG 1 cut(s) 155
BsiHKAI GWGCWC 2 cut(s) 258, 576
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 3 cut(s) 316, 340, 535
BsiWI CGTACG 1 cut(s) 156
BslFI GGGAC 1 cut(s) 671
BslI CCNNNNNNNGG 3 cut(s) 116, 117, 854
BsmAI GTCTC 2 cut(s) 38, 267
BsmBI CGTCTC 1 cut(s) 267
BsmFI GGGAC 1 cut(s) 671
BsmI GAATGC 1 cut(s) 894
BsnI GGCC 2 cut(s) 154, 815
BsoBI CYCGRG 1 cut(s) 551
Bsp1286I GDGCHC 3 cut(s) 123, 258, 576
Bsp143I GATC 3 cut(s) 226, 415, 949
BspANI GGCC 2 cut(s) 154, 815
BspCNI CTCAG 5 cut(s) 135, 226, 399, 679, 976
BspFNI CGCG 4 cut(s) 185, 327, 587, 694
BspHI TCATGA 2 cut(s) 291, 1027
BspLI GGNNCC 3 cut(s) 49, 337, 347
BspMAI CTGCAG 1 cut(s) 892
BspMI ACCTGC 1 cut(s) 17
BspPI GGATC 2 cut(s) 221, 410
BspT107I GGYRCC 1 cut(s) 335
BsrDI GCAATG 2 cut(s) 835, 1020
BsrI ACTGG 2 cut(s) 408, 761
BssECI CCNNGG 5 cut(s) 183, 249, 816, 934, 1064
BssMI GATC 3 cut(s) 226, 415, 949
BssNI GRCGYC 2 cut(s) 279, 336
BssT1I CCWWGG 1 cut(s) 1064
Bst2UI CCWGG 2 cut(s) 818, 935
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 2 cut(s) 43, 957
BstACI GRCGYC 2 cut(s) 279, 336
BstBAI YACGTR 1 cut(s) 721
BstC8I GCNNGC 2 cut(s) 325, 892
BstDEI CTNAG 5 cut(s) 122, 234, 386, 666, 984
BstDSI CCRYGG 2 cut(s) 183, 249
BstF5I GGATG 4 cut(s) 69, 436, 732, 736
BstFNI CGCG 4 cut(s) 185, 327, 587, 694
BstH2I RGCGCY 2 cut(s) 307, 339
BstHHI GCGC 2 cut(s) 306, 338
BstKTI GATC 3 cut(s) 229, 418, 952
BstMAI GTCTC 2 cut(s) 38, 267
BstMBI GATC 3 cut(s) 226, 415, 949
BstMCI CGRYCG 1 cut(s) 155
BstMWI GCNNNNNNNGC 3 cut(s) 179, 182, 333
BstNI CCWGG 2 cut(s) 818, 935
BstSCI CCNGG 3 cut(s) 338, 816, 933
BstSFI CTRYAG 1 cut(s) 888
BstUI CGCG 4 cut(s) 185, 327, 587, 694
BstV1I GCAGC 5 cut(s) 185, 317, 402, 874, 1061
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
BstZI CGGCCG 1 cut(s) 152
BsuRI GGCC 2 cut(s) 154, 815
BtgI CCRYGG 2 cut(s) 183, 249
BtgZI GCGATG 1 cut(s) 675
BtsCI GGATG 4 cut(s) 69, 436, 732, 736
BveI ACCTGC 1 cut(s) 17
Cac8I GCNNGC 2 cut(s) 325, 892
CaiI CAGNNNCTG 1 cut(s) 887
CciI TCATGA 2 cut(s) 291, 1027
CfoI GCGC 2 cut(s) 306, 338
Cfr13I GGNCC 4 cut(s) 318, 537, 759, 814
Cfr42I CCGCGG 1 cut(s) 186
CpoI CGGWCCG 2 cut(s) 318, 537
CseI GACGC 4 cut(s) 554, 576, 593, 986
Csp6I GTAC 4 cut(s) 157, 352, 421, 598
CspCI CAANNNNNGTGG 2 cut(s) 702, 737
CspI CGGWCCG 2 cut(s) 318, 537
CviQI GTAC 4 cut(s) 157, 352, 421, 598
DdeI CTNAG 5 cut(s) 122, 234, 386, 666, 984
DinI GGCGCC 1 cut(s) 337
DpnI GATC 3 cut(s) 228, 417, 951
DpnII GATC 3 cut(s) 226, 415, 949
EaeI YGGCCR 1 cut(s) 152
EagI CGGCCG 1 cut(s) 152
Eam1104I CTCTTC 2 cut(s) 43, 957
EarI CTCTTC 2 cut(s) 43, 957
EciI GGCGGA 1 cut(s) 529
EclXI CGGCCG 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 1064
Eco24I GRGCYC 1 cut(s) 123
Eco47I GGWCC 3 cut(s) 318, 537, 759
Eco52I CGGCCG 1 cut(s) 152
Eco57I CTGAAG 1 cut(s) 981
Eco72I CACGTG 1 cut(s) 721
Eco88I CYCGRG 1 cut(s) 551
EcoO109I RGGNCCY 1 cut(s) 814
EcoRI GAATTC 1 cut(s) 1009
EcoRII CCWGG 2 cut(s) 816, 933
EcoT14I CCWWGG 1 cut(s) 1064
EcoT38I GRGCYC 1 cut(s) 123
EgeI GGCGCC 1 cut(s) 337
EheI GGCGCC 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 1064
Esp3I CGTCTC 1 cut(s) 267
FaqI GGGAC 1 cut(s) 671
FokI GGATG 4 cut(s) 56, 443, 739, 743
FriOI GRGCYC 1 cut(s) 123
FspBI CTAG 2 cut(s) 20, 224
GlaI GCGC 2 cut(s) 305, 337
HaeII RGCGCY 2 cut(s) 307, 339
HaeIII GGCC 2 cut(s) 154, 815
HapII CCGG 3 cut(s) 316, 340, 535
HgaI GACGC 4 cut(s) 554, 576, 593, 986
HhaI GCGC 2 cut(s) 306, 338
Hin1I GRCGYC 2 cut(s) 279, 336
Hin6I GCGC 2 cut(s) 304, 336
HinP1I GCGC 2 cut(s) 304, 336
HinfI GANTC 5 cut(s) 236, 363, 556, 659, 866
HpaII CCGG 3 cut(s) 316, 340, 535
HphI GGTGA 4 cut(s) 99, 407, 472, 959
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 9 cut(s) 59, 125, 235, 471, 785, 829, 954, 961, 1073
Hpy188III TCNNGA 4 cut(s) 292, 553, 668, 1028
Hpy8I GTNNAC 1 cut(s) 25
Hpy99I CGWCG 3 cut(s) 281, 284, 548
HpyAV CCTTC 2 cut(s) 156, 1039
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 2 cut(s) 279, 720
HpyCH4V TGCA 7 cut(s) 12, 773, 835, 840, 890, 904, 1037
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 182, 333
HpyF3I CTNAG 5 cut(s) 122, 234, 386, 666, 984
HpySE526I ACGT 2 cut(s) 279, 720
Hsp92I GRCGYC 2 cut(s) 279, 336
HspAI GCGC 2 cut(s) 304, 336
KasI GGCGCC 1 cut(s) 335
KspI CCGCGG 1 cut(s) 186
Kzo9I GATC 3 cut(s) 226, 415, 949
LmnI GCTCC 6 cut(s) 148, 208, 253, 301, 471, 626
Lsp1109I GCAGC 5 cut(s) 185, 317, 402, 874, 1061
LweI GCATC 1 cut(s) 721
MaeI CTAG 2 cut(s) 20, 224
MaeII ACGT 2 cut(s) 279, 720
MaeIII GTNAC 2 cut(s) 237, 581
MalI GATC 3 cut(s) 228, 417, 951
MboI GATC 3 cut(s) 226, 415, 949
MboII GAAGA 3 cut(s) 30, 968, 974
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 1 cut(s) 226
MhlI GDGCHC 3 cut(s) 123, 258, 576
MluCI AATT 6 cut(s) 65, 165, 705, 972, 1009, 1014
MluI ACGCGT 1 cut(s) 585
Mly113I GGCGCC 1 cut(s) 336
MlyI GAGTC 2 cut(s) 245, 653
MmeI TCCRAC 3 cut(s) 82, 449, 763
MslI CAYNNNNRTG 3 cut(s) 296, 899, 1032
MspA1I CMGCKG 2 cut(s) 185, 393
MspI CCGG 3 cut(s) 316, 340, 535
MspR9I CCNGG 3 cut(s) 340, 818, 935
MunI CAATTG 1 cut(s) 165
Mva1269I GAATGC 1 cut(s) 894
MvaI CCWGG 2 cut(s) 818, 935
MvnI CGCG 4 cut(s) 185, 327, 587, 694
MwoI GCNNNNNNNGC 3 cut(s) 179, 182, 333
NarI GGCGCC 1 cut(s) 336
NciI CCSGG 1 cut(s) 340
NdeII GATC 3 cut(s) 226, 415, 949
NlaIV GGNNCC 3 cut(s) 49, 337, 347
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 2 cut(s) 237, 581
PagI TCATGA 2 cut(s) 291, 1027
PctI GAATGC 1 cut(s) 894
PfeI GAWTC 3 cut(s) 363, 556, 866
Pfl23II CGTACG 1 cut(s) 156
PflFI GACNNNGTC 1 cut(s) 278
PleI GAGTC 2 cut(s) 244, 653
PluTI GGCGCC 1 cut(s) 339
PmaCI CACGTG 1 cut(s) 721
PmlI CACGTG 1 cut(s) 721
PpsI GAGTC 2 cut(s) 244, 653
Ppu21I YACGTR 1 cut(s) 721
Psp6I CCWGG 2 cut(s) 816, 933
PspCI CACGTG 1 cut(s) 721
PspGI CCWGG 2 cut(s) 816, 933
PspLI CGTACG 1 cut(s) 156
PspN4I GGNNCC 3 cut(s) 49, 337, 347
PspPI GGNCC 4 cut(s) 318, 537, 759, 814
PstI CTGCAG 1 cut(s) 892
PstNI CAGNNNCTG 1 cut(s) 887
PsuI RGATCY 1 cut(s) 226
PsyI GACNNNGTC 1 cut(s) 278
RsaI GTAC 4 cut(s) 158, 353, 422, 599
RsaNI GTAC 4 cut(s) 157, 352, 421, 598
RseI CAYNNNNRTG 3 cut(s) 296, 899, 1032
Rsr2I CGGWCCG 2 cut(s) 318, 537
RsrII CGGWCCG 2 cut(s) 318, 537
SacII CCGCGG 1 cut(s) 186
Sau3AI GATC 3 cut(s) 226, 415, 949
Sau96I GGNCC 4 cut(s) 318, 537, 759, 814
SchI GAGTC 2 cut(s) 245, 653
ScrFI CCNGG 3 cut(s) 340, 818, 935
SduI GDGCHC 3 cut(s) 123, 258, 576
SfaNI GCATC 1 cut(s) 721
SfcI CTRYAG 1 cut(s) 888
SfoI GGCGCC 1 cut(s) 337
Sfr303I CCGCGG 1 cut(s) 186
SgrBI CCGCGG 1 cut(s) 186
SinI GGWCC 3 cut(s) 318, 537, 759
SmiMI CAYNNNNRTG 3 cut(s) 296, 899, 1032
Sse9I AATT 6 cut(s) 65, 165, 705, 972, 1009, 1014
SspDI GGCGCC 1 cut(s) 335
SspMI CTAG 2 cut(s) 20, 224
StyD4I CCNGG 3 cut(s) 338, 816, 933
StyI CCWWGG 1 cut(s) 1064
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 2 cut(s) 282, 723
TaqI TCGA 2 cut(s) 357, 703
TasI AATT 6 cut(s) 65, 165, 705, 972, 1009, 1014
TauI GCSGC 5 cut(s) 185, 309, 330, 336, 375
TfiI GAWTC 3 cut(s) 363, 556, 866
TseFI GTSAC 2 cut(s) 237, 581
TseI GCWGC 5 cut(s) 173, 330, 390, 887, 1049
Tsp45I GTSAC 2 cut(s) 237, 581
TspDTI ATGAA 6 cut(s) 58, 280, 308, 557, 804, 866
TspGWI ACGGA 1 cut(s) 408
Tth111I GACNNNGTC 1 cut(s) 278
VpaK11BI GGWCC 3 cut(s) 318, 537, 759
XapI RAATTY 2 cut(s) 972, 1009
XcmI CCANNNNNNNNNTGG 1 cut(s) 718
XspI CTAG 2 cut(s) 20, 224
ZraI GACGTC 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.