AT5G16100

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006657
Physical Location & Seq
Reverse (-)
5257609 .. 5259924
2316 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16100.2

Sequence Viewer

Length: 1074 bp
ATGGCGGGTGATGATCCAAAATCATCCGCTCCTAAAGACGATGGGTATGAAGATCCCTACATTAATGATCCATTTGATGGGTTCGGGCCCTTTACGGACGAAGATCTAATTAATGTAATGGCCGACATAAACCCAAACTTAATGGACATGTCTTTCACGGAACACACGCCTCCGTCTGTGTTACCTACATTACCATCGGACGTGGACGAAACTCATTTGTTTCATGAACTCGATCTTTCGATCAGTAACTACGACTCCAAAGATGAGTTCGAGACACTGTCTTTCGTTGAAGACGGTTTCACCATCCCCTTGGATATGTTCGAACAACCTAATGATGAACATAACGACAACAATGATGTCCCAGGAGAGATTGGTAATGATAATGGTAAAAACGTTGTTGGTGATAGTACTCGACTTGAATTGAGCACTAGAGTTTTTGGTGGTGCTAATGGTCTAGACTTTGAAATTGGTGGTTCAGCAACAATGCCTGTCCCGGTTACAACGACAATCTCTGATGGAGTTCACGCATGCAATTGTTGCAGATTGCTTCGAGAATTAGTCCATCTCAAGGAAGGGGAGGAAAGGAGTAAGCTTGACATCTATGGAGGAATTGGTTTCATTTGCCATGCTATTCTCTTGATTCAACTGTTGGCTCCTGATTCTATGCAACGTCAACCTCTAATATTTCATCTGCAGCATTTAACGATGGAGGAAGTTAAAAAGTTCATAGAAGATTATTGCTACCAGAGAGTAGCAAATGGTTTATCACTTTTGCAAGACACTAATGCTGCTTTTTATCAAGCAATGAGTGCCAACTCCATCTTTAACCAGCCACCACCTATGTTGACATTACCATCAAGCACTGATGTTCCACTGTCTTTGCTTTCGCCTAACGAAGATCACGATGTTCCACTGTCTTTGCTTTCGCCTGAGGAAGCCCTAGATATTCAGCCAGTTCCACCACATGTTGAACTAAGGGAGGAACCCACCACCAGGTTGCCGAAACAAAGGGAAAAGCGACAAACACCTCTTGCTGCACAGGTTATATCTCTAGTTTCTCAATTTAATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

357

Amino Acids

39.59

Weight (kDa)

4.29

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 353 - 401 1.4e-22 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 77
AccBSI CCGCTC 1 cut(s) 29
AciI CCGC 2 cut(s) 5, 27
AclI AACGTT 1 cut(s) 393
AclWI GGATC 3 cut(s) 8, 47, 62
AcoI YGGCCR 1 cut(s) 120
AfaI GTAC 1 cut(s) 409
AfiI CCNNNNNNNGG 3 cut(s) 77, 568, 993
AflIII ACRYGT 2 cut(s) 147, 964
AgsI TTSAA 5 cut(s) 290, 419, 464, 644, 971
AjiI CACGTC 1 cut(s) 202
AjnI CCWGG 2 cut(s) 361, 992
AjuI GAANNNNNNNTTGG 2 cut(s) 251, 283
AluBI AGCT 1 cut(s) 592
AluI AGCT 1 cut(s) 592
Alw21I GWGCWC 1 cut(s) 428
Alw26I GTCTC 1 cut(s) 266
AlwI GGATC 3 cut(s) 8, 47, 62
AoxI GGCC 2 cut(s) 86, 120
ApaI GGGCCC 1 cut(s) 90
ApeKI GCWGC 3 cut(s) 694, 788, 1034
AseI ATTAAT 2 cut(s) 63, 111
AspS9I GGNCC 2 cut(s) 86, 87
AsuC2I CCSGG 1 cut(s) 494
AsuHPI GGTGA 3 cut(s) 20, 292, 413
AsuII TTCGAA 1 cut(s) 321
AxyI CCTNAGG 1 cut(s) 930
BaeGI GKGCMC 1 cut(s) 90
BanII GRGCYC 1 cut(s) 90
BbsI GAAGAC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 428
BbvI GCAGC 3 cut(s) 706, 775, 1021
BccI CCATC 9 cut(s) 35, 71, 202, 311, 509, 570, 700, 827, 862
BciT130I CCWGG 2 cut(s) 363, 994
BcnI CCSGG 1 cut(s) 494
BcoDI GTCTC 1 cut(s) 266
BfaI CTAG 4 cut(s) 429, 455, 941, 1052
BfmI CTRYAG 1 cut(s) 692
BglII AGATCT 1 cut(s) 103
BisI GCNGC 3 cut(s) 695, 789, 1035
BlsI GCNGC 3 cut(s) 696, 790, 1036
BmcAI AGTACT 1 cut(s) 409
Bme1390I CCNGG 3 cut(s) 363, 494, 994
BmgBI CACGTC 1 cut(s) 202
BmgT120I GGNCC 2 cut(s) 86, 87
BmiI GGNNCC 3 cut(s) 88, 654, 984
BmrFI CCNGG 3 cut(s) 363, 494, 994
BpiI GAAGAC 1 cut(s) 297
Bpu14I TTCGAA 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 551
BpuMI CCSGG 1 cut(s) 494
BsaJI CCNNGG 2 cut(s) 309, 361
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 568, 993
Bse1I ACTGG 1 cut(s) 953
Bse21I CCTNAGG 1 cut(s) 930
Bse3DI GCAATG 1 cut(s) 810
BseBI CCWGG 2 cut(s) 363, 994
BseDI CCNNGG 2 cut(s) 309, 361
BseGI GGATG 2 cut(s) 23, 303
BseLI CCNNNNNNNGG 3 cut(s) 77, 568, 993
BseMI GCAATG 1 cut(s) 810
BseMII CTCAG 1 cut(s) 921
BseNI ACTGG 1 cut(s) 953
BseSI GKGCMC 1 cut(s) 90
BseXI GCAGC 3 cut(s) 706, 775, 1021
BsgI GTGCAG 1 cut(s) 1020
BshFI GGCC 2 cut(s) 88, 122
BsiHKAI GWGCWC 1 cut(s) 428
BsiSI CCGG 1 cut(s) 494
BslFI GGGAC 2 cut(s) 344, 476
BslI CCNNNNNNNGG 3 cut(s) 77, 568, 993
BsmAI GTCTC 1 cut(s) 266
BsmFI GGGAC 2 cut(s) 344, 476
BsnI GGCC 2 cut(s) 88, 122
Bsp119I TTCGAA 1 cut(s) 321
Bsp120I GGGCCC 1 cut(s) 86
Bsp1286I GDGCHC 2 cut(s) 90, 428
Bsp143I GATC 7 cut(s) 13, 52, 67, 103, 232, 240, 898
BspACI CCGC 2 cut(s) 5, 27
BspANI GGCC 2 cut(s) 88, 122
BspCNI CTCAG 1 cut(s) 922
BspHI TCATGA 1 cut(s) 223
BspLI GGNNCC 3 cut(s) 88, 654, 984
BspMAI CTGCAG 1 cut(s) 696
BspPI GGATC 3 cut(s) 8, 47, 62
BspT104I TTCGAA 1 cut(s) 321
BsrBI CCGCTC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 810
BsrI ACTGG 1 cut(s) 953
BssECI CCNNGG 2 cut(s) 309, 361
BssMI GATC 7 cut(s) 13, 52, 67, 103, 232, 240, 898
BssT1I CCWWGG 1 cut(s) 309
Bst2UI CCWGG 2 cut(s) 363, 994
Bst4CI ACNGT 5 cut(s) 279, 296, 648, 876, 915
BstAPI GCANNNNNTGC 2 cut(s) 537, 809
BstBI TTCGAA 1 cut(s) 321
BstC8I GCNNGC 1 cut(s) 529
BstDEI CTNAG 2 cut(s) 930, 974
BstF5I GGATG 2 cut(s) 23, 303
BstKTI GATC 7 cut(s) 16, 55, 70, 106, 235, 243, 901
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 7 cut(s) 13, 52, 67, 103, 232, 240, 898
BstMWI GCNNNNNNNGC 2 cut(s) 537, 809
BstNI CCWGG 2 cut(s) 363, 994
BstNSI RCATGY 3 cut(s) 151, 531, 968
BstSCI CCNGG 3 cut(s) 361, 492, 992
BstSFI CTRYAG 1 cut(s) 692
BstSLI GKGCMC 1 cut(s) 90
BstV1I GCAGC 3 cut(s) 706, 775, 1021
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 2 cut(s) 52, 103
BstXI CCANNNNNNTGG 1 cut(s) 310
BstYI RGATCY 2 cut(s) 52, 103
Bsu36I CCTNAGG 1 cut(s) 930
BsuRI GGCC 2 cut(s) 88, 122
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 2 cut(s) 23, 303
BtsIMutI CAGTG 4 cut(s) 275, 861, 872, 911
Cac8I GCNNGC 1 cut(s) 529
CciI TCATGA 1 cut(s) 223
Cfr13I GGNCC 2 cut(s) 86, 87
CsiI ACCWGGT 1 cut(s) 992
Csp6I GTAC 1 cut(s) 408
CspCI CAANNNNNGTGG 6 cut(s) 825, 860, 861, 896, 900, 935
CviAII CATG 5 cut(s) 148, 224, 528, 626, 965
CviJI RGCY 7 cut(s) 88, 122, 592, 653, 832, 938, 952
CviKI_1 RGCY 7 cut(s) 88, 122, 592, 653, 832, 938, 952
CviQI GTAC 1 cut(s) 408
DdeI CTNAG 2 cut(s) 930, 974
DpnI GATC 7 cut(s) 15, 54, 69, 105, 234, 242, 900
DpnII GATC 7 cut(s) 13, 52, 67, 103, 232, 240, 898
EaeI YGGCCR 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 309
Eco24I GRGCYC 1 cut(s) 90
Eco81I CCTNAGG 1 cut(s) 930
EcoO109I RGGNCCY 1 cut(s) 87
EcoRII CCWGG 2 cut(s) 361, 992
EcoT14I CCWWGG 1 cut(s) 309
EcoT38I GRGCYC 1 cut(s) 90
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 5 cut(s) 151, 227, 531, 629, 968
FaqI GGGAC 2 cut(s) 344, 476
FatI CATG 5 cut(s) 147, 223, 527, 625, 964
Fnu4HI GCNGC 3 cut(s) 695, 789, 1035
FokI GGATG 2 cut(s) 10, 290
FriOI GRGCYC 1 cut(s) 90
Fsp4HI GCNGC 3 cut(s) 695, 789, 1035
FspBI CTAG 4 cut(s) 429, 455, 941, 1052
GluI GCNGC 3 cut(s) 695, 789, 1035
HaeIII GGCC 2 cut(s) 88, 122
HapII CCGG 1 cut(s) 494
Hin1II CATG 5 cut(s) 151, 227, 531, 629, 968
HincII GTYRAC 2 cut(s) 674, 846
HindII GTYRAC 2 cut(s) 674, 846
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 3 cut(s) 254, 640, 659
HpaII CCGG 1 cut(s) 494
HphI GGTGA 3 cut(s) 20, 292, 413
Hpy166II GTNNAC 4 cut(s) 205, 523, 674, 846
Hpy188I TCNGA 2 cut(s) 199, 514
Hpy188III TCNNGA 7 cut(s) 224, 271, 455, 551, 637, 656, 902
Hpy8I GTNNAC 4 cut(s) 205, 523, 674, 846
HpyAV CCTTC 1 cut(s) 566
HpyCH4III ACNGT 5 cut(s) 279, 296, 648, 876, 915
HpyCH4IV ACGT 3 cut(s) 201, 393, 670
HpyCH4V TGCA 6 cut(s) 531, 540, 667, 694, 775, 1037
HpyF10VI GCNNNNNNNGC 2 cut(s) 537, 809
HpyF3I CTNAG 2 cut(s) 930, 974
HpySE526I ACGT 3 cut(s) 201, 393, 670
Hsp92II CATG 5 cut(s) 151, 227, 531, 629, 968
Kzo9I GATC 7 cut(s) 13, 52, 67, 103, 232, 240, 898
LmnI GCTCC 2 cut(s) 34, 658
Lsp1109I GCAGC 3 cut(s) 706, 775, 1021
MabI ACCWGGT 1 cut(s) 992
MaeI CTAG 4 cut(s) 429, 455, 941, 1052
MaeII ACGT 3 cut(s) 201, 393, 670
MaeIII GTNAC 3 cut(s) 180, 245, 496
MalI GATC 7 cut(s) 15, 54, 69, 105, 234, 242, 900
MbiI CCGCTC 1 cut(s) 29
MboI GATC 7 cut(s) 13, 52, 67, 103, 232, 240, 898
MboII GAAGA 5 cut(s) 62, 113, 302, 743, 908
MfeI CAATTG 1 cut(s) 532
MflI RGATCY 2 cut(s) 52, 103
MhlI GDGCHC 2 cut(s) 90, 428
MluCI AATT 7 cut(s) 108, 419, 465, 532, 554, 609, 1061
MlyI GAGTC 1 cut(s) 248
MnlI CCTC 8 cut(s) 180, 571, 599, 687, 703, 925, 973, 1038
MseI TTAA 7 cut(s) 63, 111, 140, 701, 717, 825, 1065
MspI CCGG 1 cut(s) 494
MspR9I CCNGG 3 cut(s) 363, 494, 994
MunI CAATTG 1 cut(s) 532
MvaI CCWGG 2 cut(s) 363, 994
MwoI GCNNNNNNNGC 2 cut(s) 537, 809
NciI CCSGG 1 cut(s) 494
NdeII GATC 7 cut(s) 13, 52, 67, 103, 232, 240, 898
NlaIII CATG 5 cut(s) 151, 227, 531, 629, 968
NlaIV GGNNCC 3 cut(s) 88, 654, 984
NspI RCATGY 3 cut(s) 151, 531, 968
NspV TTCGAA 1 cut(s) 321
PaeI GCATGC 1 cut(s) 531
PagI TCATGA 1 cut(s) 223
PciI ACATGT 2 cut(s) 147, 964
PcsI WCGNNNNNNNCGW 1 cut(s) 900
PfeI GAWTC 2 cut(s) 640, 659
PflFI GACNNNGTC 1 cut(s) 277
PflMI CCANNNNNTGG 1 cut(s) 77
PkrI GCNGC 3 cut(s) 696, 790, 1036
PleI GAGTC 1 cut(s) 248
PpsI GAGTC 1 cut(s) 248
PscI ACATGT 2 cut(s) 147, 964
PshBI ATTAAT 2 cut(s) 63, 111
Psp1406I AACGTT 1 cut(s) 393
Psp6I CCWGG 2 cut(s) 361, 992
PspGI CCWGG 2 cut(s) 361, 992
PspN4I GGNNCC 3 cut(s) 88, 654, 984
PspOMI GGGCCC 1 cut(s) 86
PspPI GGNCC 2 cut(s) 86, 87
PstI CTGCAG 1 cut(s) 696
PsuI RGATCY 2 cut(s) 52, 103
PsyI GACNNNGTC 1 cut(s) 277
RsaI GTAC 1 cut(s) 409
RsaNI GTAC 1 cut(s) 408
SaqAI TTAA 7 cut(s) 63, 111, 140, 701, 717, 825, 1065
SatI GCNGC 3 cut(s) 695, 789, 1035
Sau3AI GATC 7 cut(s) 13, 52, 67, 103, 232, 240, 898
Sau96I GGNCC 2 cut(s) 86, 87
ScaI AGTACT 1 cut(s) 409
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 3 cut(s) 363, 494, 994
SduI GDGCHC 2 cut(s) 90, 428
SexAI ACCWGGT 1 cut(s) 992
SfcI CTRYAG 1 cut(s) 692
SfuI TTCGAA 1 cut(s) 321
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
SphI GCATGC 1 cut(s) 531
Sse9I AATT 7 cut(s) 108, 419, 465, 532, 554, 609, 1061
SsiI CCGC 2 cut(s) 5, 27
SspI AATATT 1 cut(s) 684
SspMI CTAG 4 cut(s) 429, 455, 941, 1052
StyD4I CCNGG 3 cut(s) 361, 492, 992
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 5 cut(s) 279, 296, 648, 876, 915
TaiI ACGT 3 cut(s) 204, 396, 673
TaqI TCGA 6 cut(s) 231, 239, 270, 321, 412, 550
TasI AATT 7 cut(s) 108, 419, 465, 532, 554, 609, 1061
TatI WGTACW 1 cut(s) 407
TfiI GAWTC 2 cut(s) 640, 659
Tru1I TTAA 7 cut(s) 63, 111, 140, 701, 717, 825, 1065
Tru9I TTAA 7 cut(s) 63, 111, 140, 701, 717, 825, 1065
TscAI CASTG 4 cut(s) 282, 868, 879, 918
TseI GCWGC 3 cut(s) 694, 788, 1034
TspDTI ATGAA 7 cut(s) 63, 212, 240, 351, 607, 677, 715
TspGWI ACGGA 3 cut(s) 110, 162, 173
TspRI CASTG 4 cut(s) 282, 868, 879, 918
Tth111I GACNNNGTC 1 cut(s) 277
Van91I CCANNNNNTGG 1 cut(s) 77
VspI ATTAAT 2 cut(s) 63, 111
XbaI TCTAGA 1 cut(s) 454
XceI RCATGY 3 cut(s) 151, 531, 968
XspI CTAG 4 cut(s) 429, 455, 941, 1052
ZrmI AGTACT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.