Rorug01G0170100

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006657
Physical Location & Seq
Reverse (-)
23126618 .. 23127085
468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0170100.1

Sequence Viewer

Length: 468 bp
ATGTCATCGAGTTTCAGCAGTTCAAGAAGTGGCAGCGGCATGGTGGTACCCATGGAAGAGGTACAGAAGGTCTTCAACAAGTTCGACGAGAATGGTGACGGACTCATCTCTTGCGATGAGCTCAAGAACGTTTTGTGTGAGCTGGGGTGTGAGGTGAAGTCGGAGGGAGTGCAGGCTGTGATGTCCGAGTTCGACAAGGACGGCGACGGCCACATCGACCTGGAGGAGTTCACAAAACTCATGAATGGATCTGCGTCAAGCAAGGATATCCGTGACGCCTTCGATGTCTTCGATCTCGACAAGAACGGCCTGATCTCGGCTAGTGAACTTCATGAGGTGCTCAACCGCTTAGGCTTAGGGCAGAAGTGCTCCGTCGGGGAATGTGCCAGCATGATCACCAAATTTGACGCCGATGGAGATGGCTTCGTTAACTTTCAAGAGTTCAAGAATATGATGAACCGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

155

Amino Acids

16.93

Weight (kDa)

4.41

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 19 - 81 4.7e-16 EF-hand domain pair
EF-hand_1 PF00036 20 - 45 9.4e-07 EF hand domain
EF-hand_6 PF13405 21 - 49 3.6e-07 EF-hand domain
EF-hand_8 PF13833 54 - 82 1e-05 EF-hand domain pair
EF-hand_1 PF00036 57 - 82 4.4e-08 EF hand domain
EF-hand_5 PF13202 58 - 81 2.5e-06 EF hand
EF-hand_7 PF13499 88 - 152 5.5e-15 EF-hand domain pair
EF-hand_1 PF00036 89 - 116 2.2e-06 EF hand domain
EF-hand_6 PF13405 89 - 118 4.5e-07 EF-hand domain
EF-hand_5 PF13202 90 - 113 3.8e-06 EF hand
EF-hand_8 PF13833 119 - 154 2.1e-06 EF-hand domain pair
EF-hand_1 PF00036 128 - 154 2.4e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 46
AccB1I GGYRCC 1 cut(s) 46
AccBSI CCGCTC 1 cut(s) 462
AciI CCGC 3 cut(s) 36, 346, 460
AclI AACGTT 1 cut(s) 129
AclWI GGATC 1 cut(s) 256
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 1 cut(s) 401
AcyI GRCGYC 2 cut(s) 276, 408
AfaI GTAC 2 cut(s) 48, 63
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 4 cut(s) 24, 76, 437, 445
AjnI CCWGG 1 cut(s) 219
AluBI AGCT 2 cut(s) 121, 142
AluI AGCT 2 cut(s) 121, 142
Alw21I GWGCWC 3 cut(s) 123, 342, 371
AlwI GGATC 1 cut(s) 256
AoxI GGCC 2 cut(s) 208, 307
ApeKI GCWGC 1 cut(s) 33
ApoI RAATTY 1 cut(s) 401
Asp700I GAANNNNTTC 1 cut(s) 71
Asp718I GGTACC 1 cut(s) 46
AsuHPI GGTGA 3 cut(s) 107, 166, 388
BanI GGYRCC 1 cut(s) 46
BanII GRGCYC 1 cut(s) 123
BbsI GAAGAC 2 cut(s) 64, 280
Bbv12I GWGCWC 3 cut(s) 123, 342, 371
BbvI GCAGC 1 cut(s) 45
BccI CCATC 2 cut(s) 407, 413
BceAI ACGGC 3 cut(s) 217, 223, 322
BciT130I CCWGG 1 cut(s) 221
BclI TGATCA 1 cut(s) 393
BfaI CTAG 1 cut(s) 321
BisI GCNGC 2 cut(s) 34, 37
BlsI GCNGC 2 cut(s) 35, 38
Bme1390I CCNGG 1 cut(s) 221
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 1 cut(s) 221
BpiI GAAGAC 2 cut(s) 64, 280
BpmI CTGGAG 1 cut(s) 242
Bpu10I CCTNAGC 2 cut(s) 349, 355
BpuEI CTTGAG 1 cut(s) 107
BsaHI GRCGYC 2 cut(s) 276, 408
BsaJI CCNNGG 1 cut(s) 51
Bsc4I CCNNNNNNNGG 1 cut(s) 316
BseBI CCWGG 1 cut(s) 221
BseDI CCNNGG 1 cut(s) 51
BseLI CCNNNNNNNGG 1 cut(s) 316
BseRI GAGGAG 1 cut(s) 239
BseXI GCAGC 1 cut(s) 45
BseYI CCCAGC 1 cut(s) 142
BsgI GTGCAG 1 cut(s) 191
BshFI GGCC 2 cut(s) 210, 309
BshNI GGYRCC 1 cut(s) 46
BsiHKAI GWGCWC 3 cut(s) 123, 342, 371
BslI CCNNNNNNNGG 1 cut(s) 316
BsnI GGCC 2 cut(s) 210, 309
Bsp1286I GDGCHC 3 cut(s) 123, 342, 371
Bsp143I GATC 4 cut(s) 248, 292, 312, 393
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 3 cut(s) 36, 346, 460
BspANI GGCC 2 cut(s) 210, 309
BspHI TCATGA 2 cut(s) 240, 331
BspLI GGNNCC 1 cut(s) 48
BspPI GGATC 1 cut(s) 256
BspT107I GGYRCC 1 cut(s) 46
BsrBI CCGCTC 1 cut(s) 462
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 4 cut(s) 248, 292, 312, 393
BssNI GRCGYC 2 cut(s) 276, 408
BssT1I CCWWGG 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 221
Bst6I CTCTTC 1 cut(s) 51
BstACI GRCGYC 2 cut(s) 276, 408
BstC8I GCNNGC 2 cut(s) 174, 388
BstDEI CTNAG 2 cut(s) 349, 355
BstDSI CCRYGG 1 cut(s) 51
BstKTI GATC 4 cut(s) 251, 295, 315, 396
BstMBI GATC 4 cut(s) 248, 292, 312, 393
BstNI CCWGG 1 cut(s) 221
BstSCI CCNGG 1 cut(s) 219
BstV1I GCAGC 1 cut(s) 45
BstV2I GAAGAC 2 cut(s) 64, 280
BstX2I RGATCY 1 cut(s) 248
BstYI RGATCY 1 cut(s) 248
BsuRI GGCC 2 cut(s) 210, 309
BtgI CCRYGG 1 cut(s) 51
BtgZI GCGATG 1 cut(s) 129
Cac8I GCNNGC 2 cut(s) 174, 388
CciI TCATGA 2 cut(s) 240, 331
CseI GACGC 3 cut(s) 243, 284, 416
Csp6I GTAC 2 cut(s) 47, 62
CviAII CATG 5 cut(s) 40, 52, 241, 332, 391
CviJI RGCY 8 cut(s) 121, 142, 176, 210, 309, 320, 354, 423
CviKI_1 RGCY 8 cut(s) 121, 142, 176, 210, 309, 320, 354, 423
CviQI GTAC 2 cut(s) 47, 62
DdeI CTNAG 2 cut(s) 349, 355
DpnI GATC 4 cut(s) 250, 294, 314, 395
DpnII GATC 4 cut(s) 248, 292, 312, 393
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Ecl136II GAGCTC 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 123
Eco32I GATATC 1 cut(s) 268
Eco53kI GAGCTC 1 cut(s) 121
EcoICRI GAGCTC 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 219
EcoRV GATATC 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 5 cut(s) 43, 55, 244, 335, 394
FaiI YATR 6 cut(s) 41, 53, 242, 333, 392, 452
FatI CATG 5 cut(s) 39, 51, 240, 331, 390
FbaI TGATCA 1 cut(s) 393
Fnu4HI GCNGC 2 cut(s) 34, 37
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 2 cut(s) 34, 37
FspBI CTAG 1 cut(s) 321
GluI GCNGC 2 cut(s) 34, 37
GsaI CCCAGC 1 cut(s) 146
GsuI CTGGAG 1 cut(s) 242
HaeIII GGCC 2 cut(s) 210, 309
HgaI GACGC 3 cut(s) 243, 284, 416
Hin1I GRCGYC 2 cut(s) 276, 408
Hin1II CATG 5 cut(s) 43, 55, 244, 335, 394
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HinfI GANTC 1 cut(s) 102
HpaI GTTAAC 1 cut(s) 430
HphI GGTGA 3 cut(s) 107, 166, 388
Hpy166II GTNNAC 3 cut(s) 231, 326, 430
Hpy188I TCNGA 2 cut(s) 163, 187
Hpy188III TCNNGA 8 cut(s) 24, 124, 241, 296, 332, 437, 445, 465
Hpy8I GTNNAC 3 cut(s) 231, 326, 430
Hpy99I CGWCG 3 cut(s) 89, 209, 377
HpyAV CCTTC 2 cut(s) 61, 289
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 1 cut(s) 172
HpyF3I CTNAG 2 cut(s) 349, 355
HpySE526I ACGT 1 cut(s) 129
Hsp92I GRCGYC 2 cut(s) 276, 408
Hsp92II CATG 5 cut(s) 43, 55, 244, 335, 394
KpnI GGTACC 1 cut(s) 50
Ksp22I TGATCA 1 cut(s) 393
KspAI GTTAAC 1 cut(s) 430
Kzo9I GATC 4 cut(s) 248, 292, 312, 393
LmnI GCTCC 1 cut(s) 374
LpnPI CCDG 6 cut(s) 128, 158, 206, 233, 323, 400
Lsp1109I GCAGC 1 cut(s) 45
MaeI CTAG 1 cut(s) 321
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 2 cut(s) 95, 272
MalI GATC 4 cut(s) 250, 294, 314, 395
MbiI CCGCTC 1 cut(s) 462
MboI GATC 4 cut(s) 248, 292, 312, 393
MboII GAAGA 3 cut(s) 64, 68, 280
MflI RGATCY 1 cut(s) 248
MhlI GDGCHC 3 cut(s) 123, 342, 371
MluCI AATT 1 cut(s) 401
MlyI GAGTC 1 cut(s) 96
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 5 cut(s) 52, 145, 157, 217, 328
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 1 cut(s) 429
MspA1I CMGCKG 1 cut(s) 36
MspR9I CCNGG 1 cut(s) 221
MvaI CCWGG 1 cut(s) 221
NcoI CCATGG 1 cut(s) 51
NdeII GATC 4 cut(s) 248, 292, 312, 393
NlaIII CATG 5 cut(s) 43, 55, 244, 335, 394
NlaIV GGNNCC 1 cut(s) 48
NmeAIII GCCGAG 1 cut(s) 296
NmuCI GTSAC 2 cut(s) 95, 272
PagI TCATGA 2 cut(s) 240, 331
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 288
PdmI GAANNNNTTC 1 cut(s) 71
PkrI GCNGC 2 cut(s) 35, 38
PleI GAGTC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 96
Psp124BI GAGCTC 1 cut(s) 123
Psp1406I AACGTT 1 cut(s) 129
Psp6I CCWGG 1 cut(s) 219
PspFI CCCAGC 1 cut(s) 142
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 1 cut(s) 48
PsuI RGATCY 1 cut(s) 248
RsaI GTAC 2 cut(s) 48, 63
RsaNI GTAC 2 cut(s) 47, 62
SacI GAGCTC 1 cut(s) 123
SaqAI TTAA 1 cut(s) 429
SatI GCNGC 2 cut(s) 34, 37
Sau3AI GATC 4 cut(s) 248, 292, 312, 393
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 1 cut(s) 221
SduI GDGCHC 3 cut(s) 123, 342, 371
SetI ASST 8 cut(s) 63, 72, 123, 132, 144, 156, 222, 339
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 1 cut(s) 401
SsiI CCGC 3 cut(s) 36, 346, 460
SspMI CTAG 1 cut(s) 321
SstI GAGCTC 1 cut(s) 123
StyD4I CCNGG 1 cut(s) 219
StyI CCWWGG 1 cut(s) 51
TaiI ACGT 1 cut(s) 132
TaqI TCGA 7 cut(s) 8, 84, 192, 216, 282, 291, 297
TasI AATT 1 cut(s) 401
TauI GCSGC 1 cut(s) 39
Tru1I TTAA 1 cut(s) 429
Tru9I TTAA 1 cut(s) 429
TseFI GTSAC 2 cut(s) 95, 272
TseI GCWGC 1 cut(s) 33
Tsp45I GTSAC 2 cut(s) 95, 272
TspDTI ATGAA 2 cut(s) 257, 320
TspGWI ACGGA 3 cut(s) 114, 260, 361
XapI RAATTY 1 cut(s) 401
XmnI GAANNNNTTC 1 cut(s) 71
XspI CTAG 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.