Rh7DG395100

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0006657
Physical Location & Seq
Reverse (-)
54208307 .. 54210351
2045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG395100.1

Sequence Viewer

Length: 1149 bp
ATGACAGATTCTCCGTGGGCTGTTGTGCCTTACCATGACCCTTATGATCATGAAGTCCCCGACGTTGTGAATTTCATTAATGATACTCCTGATCCAACCCTTGACACATTGTTCGACGGTGGAGTAGTTCCTTCTTTCTCTCATCAATATCCAATTCCTCAGTTTGATAATGGAATCCAAGATTTTCCCATGTCCCCAATGTGGGATATGGGCAACAATTATAATGGCGGAGGGAATAATACTATTTCAGTACTAGCGGATCATCCTTCACAGTGTTCCGGACGAGGAGGAGGAGAATCGGGCTTTGATCAGAATTCGGGGTTAGGGTTTGGGAGTAATGTGATGCCACCTCCAGTTTGGGCTCCACCACCAATCCCCTTCAGTTGCTGCGGTTGCCAAGTTCTAAGGGAAATTGTTCACTTCAATCGCAATTATATTATGACACTTGAAATCCATGGAAGACTCGGTGCCATCTGTCATGCAATTCAAGAAAGCCGCGCCAATTACTTGTACGATTCCAGTGCTTCTATTGAGCCTCACTATCAGAACTTTGACTTTTGCAATAAAAACATTGAGGAAGTGAAGCAGTTTCTGACGCAATACTGCTTAGAGCGAAAGGTAGAAGGCTACATTATGCAGCAAGATCCTCTGCAAGCATTTTATCAGACTCTCTGTGTTGGTATGGATGGTGAGTGGGATGATATTCTTAGTTATCCCGACGAATTCATTCCTGAGGACTTGGAAACTACCGACGACTTCATTTCTCCTAATTCAGAAATGGCGGAAGAAATGGAACAACCTGTCGATGAGGTGACCGTGAATATGAAAGGGAAGGGTCCTAGGCTTCCCAGGTTTTCCCTTGCAGAACAGAGGGAGAGAGCAGCAAAAATGAAATTGAATGATCTTCGTGCTTACTTCCATCTCACCATTGAAGAGGCTTCTGAGCAATTGGGTTTTTGCCCAACTGTTGTCAAGAGGATCTGCCGCAAGTTTGGAGTAAGCAGGTGGCCTGCTAGAAAGATCAAAAGCATTGAGAGGCGAATATCAAGCTTGAGGCCGCTTTTGAATTCATATAGTGATAGCACCAGGGTCCATGTTGCAGCTGAAATTAAAAGGCTTGAAAGAGAAGTAGCCCGACTTGTATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

382

Amino Acids

43.32

Weight (kDa)

4.89

Isoelectric Point (pI)

52.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 296 - 344 9.9e-22 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 222
AarI CACCTGC 1 cut(s) 993
Acc36I ACCTGC 1 cut(s) 993
AccB1I GGYRCC 1 cut(s) 467
AccII CGCG 1 cut(s) 498
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 7 cut(s) 228, 257, 390, 496, 782, 985, 1058
AclWI GGATC 4 cut(s) 86, 267, 638, 986
AcsI RAATTY 4 cut(s) 70, 313, 722, 1066
AcuI CTGAAG 1 cut(s) 364
AfaI GTAC 2 cut(s) 252, 512
AfiI CCNNNNNNNGG 2 cut(s) 201, 202
AgsI TTSAA 7 cut(s) 424, 449, 488, 898, 932, 1066, 1121
AjnI CCWGG 2 cut(s) 848, 1085
AluBI AGCT 2 cut(s) 1050, 1103
AluI AGCT 2 cut(s) 1050, 1103
AlwI GGATC 4 cut(s) 86, 267, 638, 986
AlwNI CAGNNNCTG 2 cut(s) 387, 592
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 2 cut(s) 1007, 1055
ApeKI GCWGC 4 cut(s) 387, 637, 881, 1100
ApoI RAATTY 4 cut(s) 70, 313, 722, 1066
ArsI GACNNNNNNTTYG 2 cut(s) 95, 127
AseI ATTAAT 1 cut(s) 78
Asp700I GAANNNNTTC 2 cut(s) 414, 726
AspA2I CCTAGG 1 cut(s) 839
AspLEI GCGC 1 cut(s) 500
AspS9I GGNCC 2 cut(s) 836, 1090
AsuHPI GGTGA 3 cut(s) 701, 823, 916
AvaII GGWCC 2 cut(s) 836, 1090
AvrII CCTAGG 1 cut(s) 839
AxyI CCTNAGG 1 cut(s) 732
BanI GGYRCC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 364
BbsI GAAGAC 1 cut(s) 466
BbvI GCAGC 4 cut(s) 374, 649, 893, 1112
BccI CCATC 3 cut(s) 479, 680, 927
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BciT130I CCWGG 2 cut(s) 850, 1087
BclI TGATCA 2 cut(s) 46, 307
BfaI CTAG 3 cut(s) 254, 840, 1014
BfuAI ACCTGC 1 cut(s) 993
BisI GCNGC 7 cut(s) 388, 496, 638, 882, 985, 1058, 1101
BlnI CCTAGG 1 cut(s) 839
BlsI GCNGC 7 cut(s) 389, 497, 639, 883, 986, 1059, 1102
BmcAI AGTACT 1 cut(s) 252
Bme1390I CCNGG 2 cut(s) 850, 1087
Bme18I GGWCC 2 cut(s) 836, 1090
BmgT120I GGNCC 2 cut(s) 836, 1090
BmiI GGNNCC 4 cut(s) 363, 469, 837, 1091
BmrFI CCNGG 2 cut(s) 850, 1087
BmsI GCATC 1 cut(s) 333
BpiI GAAGAC 1 cut(s) 466
BpmI CTGGAG 1 cut(s) 336
BpuEI CTTGAG 1 cut(s) 1072
BsaJI CCNNGG 5 cut(s) 14, 454, 839, 848, 1086
BsaWI WCCGGW 1 cut(s) 278
BsaXI ACNNNNNCTCC 1 cut(s) 25
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 202
Bse1I ACTGG 2 cut(s) 353, 519
Bse21I CCTNAGG 1 cut(s) 732
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 2 cut(s) 850, 1087
BseDI CCNNGG 5 cut(s) 14, 454, 839, 848, 1086
BseGI GGATG 3 cut(s) 262, 691, 703
BseLI CCNNNNNNNGG 2 cut(s) 201, 202
BseMII CTCAG 3 cut(s) 173, 723, 933
BseNI ACTGG 2 cut(s) 353, 519
BseRI GAGGAG 3 cut(s) 300, 303, 306
BseXI GCAGC 4 cut(s) 374, 649, 893, 1112
Bsh1236I CGCG 1 cut(s) 498
BshFI GGCC 2 cut(s) 1009, 1057
BshNI GGYRCC 1 cut(s) 467
BsiSI CCGG 1 cut(s) 279
BslFI GGGAC 2 cut(s) 41, 178
BslI CCNNNNNNNGG 2 cut(s) 201, 202
BsmFI GGGAC 2 cut(s) 41, 178
BsnI GGCC 2 cut(s) 1009, 1057
Bsp1286I GDGCHC 1 cut(s) 364
Bsp13I TCCGGA 1 cut(s) 278
Bsp143I GATC 8 cut(s) 46, 91, 259, 307, 643, 901, 978, 1020
Bsp19I CCATGG 1 cut(s) 454
BspACI CCGC 7 cut(s) 228, 257, 390, 496, 782, 985, 1058
BspANI GGCC 2 cut(s) 1009, 1057
BspCNI CTCAG 3 cut(s) 172, 724, 934
BspEI TCCGGA 1 cut(s) 278
BspFNI CGCG 1 cut(s) 498
BspHI TCATGA 1 cut(s) 49
BspLI GGNNCC 4 cut(s) 363, 469, 837, 1091
BspMI ACCTGC 1 cut(s) 993
BspPI GGATC 4 cut(s) 86, 267, 638, 986
BspT107I GGYRCC 1 cut(s) 467
BsrI ACTGG 2 cut(s) 353, 519
BssECI CCNNGG 5 cut(s) 14, 454, 839, 848, 1086
BssMI GATC 8 cut(s) 46, 91, 259, 307, 643, 901, 978, 1020
BssT1I CCWWGG 2 cut(s) 454, 839
Bst2UI CCWGG 2 cut(s) 850, 1087
Bst4CI ACNGT 4 cut(s) 119, 273, 817, 967
Bst6I CTCTTC 1 cut(s) 927
BstC8I GCNNGC 2 cut(s) 654, 1011
BstDEI CTNAG 6 cut(s) 159, 404, 607, 707, 732, 942
BstDSI CCRYGG 2 cut(s) 14, 454
BstEII GGTNACC 1 cut(s) 811
BstF5I GGATG 3 cut(s) 262, 691, 703
BstFNI CGCG 1 cut(s) 498
BstHHI GCGC 1 cut(s) 500
BstKTI GATC 8 cut(s) 49, 94, 262, 310, 646, 904, 981, 1023
BstMBI GATC 8 cut(s) 46, 91, 259, 307, 643, 901, 978, 1020
BstMWI GCNNNNNNNGC 1 cut(s) 393
BstNI CCWGG 2 cut(s) 850, 1087
BstPI GGTNACC 1 cut(s) 811
BstSCI CCNGG 2 cut(s) 848, 1085
BstUI CGCG 1 cut(s) 498
BstV1I GCAGC 4 cut(s) 374, 649, 893, 1112
BstV2I GAAGAC 1 cut(s) 466
BstX2I RGATCY 2 cut(s) 643, 978
BstYI RGATCY 2 cut(s) 643, 978
Bsu36I CCTNAGG 1 cut(s) 732
BsuRI GGCC 2 cut(s) 1009, 1057
BtgI CCRYGG 2 cut(s) 14, 454
BtsCI GGATG 3 cut(s) 262, 691, 703
BtsIMutI CAGTG 2 cut(s) 278, 526
BveI ACCTGC 1 cut(s) 993
Cac8I GCNNGC 2 cut(s) 654, 1011
CaiI CAGNNNCTG 2 cut(s) 387, 592
CciI TCATGA 1 cut(s) 49
CfoI GCGC 1 cut(s) 500
Cfr13I GGNCC 2 cut(s) 836, 1090
CseI GACGC 1 cut(s) 604
Csp6I GTAC 2 cut(s) 251, 511
CviAII CATG 6 cut(s) 35, 50, 190, 455, 479, 1094
CviQI GTAC 2 cut(s) 251, 511
DdeI CTNAG 6 cut(s) 159, 404, 607, 707, 732, 942
DpnI GATC 8 cut(s) 48, 93, 261, 309, 645, 903, 980, 1022
DpnII GATC 8 cut(s) 46, 91, 259, 307, 643, 901, 978, 1020
Eam1104I CTCTTC 1 cut(s) 927
EarI CTCTTC 1 cut(s) 927
EciI GGCGGA 2 cut(s) 243, 797
Eco130I CCWWGG 2 cut(s) 454, 839
Eco24I GRGCYC 1 cut(s) 364
Eco47I GGWCC 2 cut(s) 836, 1090
Eco57I CTGAAG 1 cut(s) 364
Eco81I CCTNAGG 1 cut(s) 732
Eco91I GGTNACC 1 cut(s) 811
EcoO109I RGGNCCY 1 cut(s) 836
EcoO65I GGTNACC 1 cut(s) 811
EcoRI GAATTC 3 cut(s) 313, 722, 1066
EcoRII CCWGG 2 cut(s) 848, 1085
EcoT14I CCWWGG 2 cut(s) 454, 839
EcoT38I GRGCYC 1 cut(s) 364
ErhI CCWWGG 2 cut(s) 454, 839
FaeI CATG 6 cut(s) 38, 53, 193, 458, 482, 1097
FaqI GGGAC 2 cut(s) 41, 178
FatI CATG 6 cut(s) 34, 49, 189, 454, 478, 1093
FbaI TGATCA 2 cut(s) 46, 307
Fnu4HI GCNGC 7 cut(s) 388, 496, 638, 882, 985, 1058, 1101
FokI GGATG 3 cut(s) 249, 698, 710
FriOI GRGCYC 1 cut(s) 364
Fsp4HI GCNGC 7 cut(s) 388, 496, 638, 882, 985, 1058, 1101
FspBI CTAG 3 cut(s) 254, 840, 1014
GlaI GCGC 1 cut(s) 499
GluI GCNGC 7 cut(s) 388, 496, 638, 882, 985, 1058, 1101
GsuI CTGGAG 1 cut(s) 336
HaeIII GGCC 2 cut(s) 1009, 1057
HapII CCGG 1 cut(s) 279
HgaI GACGC 1 cut(s) 604
HhaI GCGC 1 cut(s) 500
Hin1II CATG 6 cut(s) 38, 53, 193, 458, 482, 1097
Hin6I GCGC 1 cut(s) 498
HinP1I GCGC 1 cut(s) 498
HindIII AAGCTT 1 cut(s) 1048
HinfI GANTC 6 cut(s) 8, 174, 296, 462, 515, 667
HpaII CCGG 1 cut(s) 279
HphI GGTGA 3 cut(s) 701, 823, 916
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 6 cut(s) 312, 546, 594, 666, 775, 943
Hpy188III TCNNGA 7 cut(s) 50, 89, 279, 488, 716, 731, 973
Hpy8I GTNNAC 1 cut(s) 418
Hpy99I CGWCG 4 cut(s) 65, 119, 722, 755
HpyAV CCTTC 5 cut(s) 141, 276, 388, 617, 826
HpyCH4III ACNGT 4 cut(s) 119, 273, 817, 967
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 6 cut(s) 482, 561, 637, 652, 863, 1100
HpyF10VI GCNNNNNNNGC 1 cut(s) 393
HpyF3I CTNAG 6 cut(s) 159, 404, 607, 707, 732, 942
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 6 cut(s) 38, 53, 193, 458, 482, 1097
HspAI GCGC 1 cut(s) 498
Kpn2I TCCGGA 1 cut(s) 278
Ksp22I TGATCA 2 cut(s) 46, 307
Kzo9I GATC 8 cut(s) 46, 91, 259, 307, 643, 901, 978, 1020
LmnI GCTCC 1 cut(s) 367
Lsp1109I GCAGC 4 cut(s) 374, 649, 893, 1112
LweI GCATC 1 cut(s) 333
MaeI CTAG 3 cut(s) 254, 840, 1014
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 1 cut(s) 811
MalI GATC 8 cut(s) 48, 93, 261, 309, 645, 903, 980, 1022
MboI GATC 8 cut(s) 46, 91, 259, 307, 643, 901, 978, 1020
MboII GAAGA 4 cut(s) 471, 797, 896, 944
MfeI CAATTG 1 cut(s) 947
MflI RGATCY 2 cut(s) 643, 978
MhlI GDGCHC 1 cut(s) 364
MlyI GAGTC 2 cut(s) 456, 661
MmeI TCCRAC 1 cut(s) 119
MroI TCCGGA 1 cut(s) 278
MroXI GAANNNNTTC 2 cut(s) 414, 726
MseI TTAA 2 cut(s) 78, 1110
MspA1I CMGCKG 1 cut(s) 1103
MspI CCGG 1 cut(s) 279
MspR9I CCNGG 2 cut(s) 850, 1087
MunI CAATTG 1 cut(s) 947
MvaI CCWGG 2 cut(s) 850, 1087
MvnI CGCG 1 cut(s) 498
MwoI GCNNNNNNNGC 1 cut(s) 393
NcoI CCATGG 1 cut(s) 454
NdeII GATC 8 cut(s) 46, 91, 259, 307, 643, 901, 978, 1020
NlaIII CATG 6 cut(s) 38, 53, 193, 458, 482, 1097
NlaIV GGNNCC 4 cut(s) 363, 469, 837, 1091
NmuCI GTSAC 1 cut(s) 811
PagI TCATGA 1 cut(s) 49
PaqCI CACCTGC 1 cut(s) 993
PdmI GAANNNNTTC 2 cut(s) 414, 726
PfeI GAWTC 4 cut(s) 8, 174, 296, 515
PkrI GCNGC 7 cut(s) 389, 497, 639, 883, 986, 1059, 1102
PleI GAGTC 2 cut(s) 456, 661
PpsI GAGTC 2 cut(s) 456, 661
PpuMI RGGWCCY 1 cut(s) 836
PshBI ATTAAT 1 cut(s) 78
PsiI TTATAA 1 cut(s) 222
Psp5II RGGWCCY 1 cut(s) 836
Psp6I CCWGG 2 cut(s) 848, 1085
PspEI GGTNACC 1 cut(s) 811
PspGI CCWGG 2 cut(s) 848, 1085
PspN4I GGNNCC 4 cut(s) 363, 469, 837, 1091
PspPI GGNCC 2 cut(s) 836, 1090
PspPPI RGGWCCY 1 cut(s) 836
PstNI CAGNNNCTG 2 cut(s) 387, 592
PsuI RGATCY 2 cut(s) 643, 978
PvuII CAGCTG 1 cut(s) 1103
RsaI GTAC 2 cut(s) 252, 512
RsaNI GTAC 2 cut(s) 251, 511
SaqAI TTAA 2 cut(s) 78, 1110
SatI GCNGC 7 cut(s) 388, 496, 638, 882, 985, 1058, 1101
Sau3AI GATC 8 cut(s) 46, 91, 259, 307, 643, 901, 978, 1020
Sau96I GGNCC 2 cut(s) 836, 1090
ScaI AGTACT 1 cut(s) 252
SchI GAGTC 2 cut(s) 456, 661
ScrFI CCNGG 2 cut(s) 850, 1087
SduI GDGCHC 1 cut(s) 364
SetI ASST 9 cut(s) 66, 352, 621, 802, 813, 854, 1007, 1052, 1105
SfaNI GCATC 1 cut(s) 333
SinI GGWCC 2 cut(s) 836, 1090
SmlI CTYRAG 1 cut(s) 1051
SmoI CTYRAG 1 cut(s) 1051
SsiI CCGC 7 cut(s) 228, 257, 390, 496, 782, 985, 1058
SspMI CTAG 3 cut(s) 254, 840, 1014
StyD4I CCNGG 2 cut(s) 848, 1085
StyI CCWWGG 2 cut(s) 454, 839
TaaI ACNGT 4 cut(s) 119, 273, 817, 967
TaiI ACGT 1 cut(s) 66
TaqI TCGA 2 cut(s) 114, 804
TatI WGTACW 1 cut(s) 250
TauI GCSGC 3 cut(s) 498, 987, 1060
TfiI GAWTC 4 cut(s) 8, 174, 296, 515
Tru1I TTAA 2 cut(s) 78, 1110
Tru9I TTAA 2 cut(s) 78, 1110
TscAI CASTG 2 cut(s) 278, 526
TseFI GTSAC 1 cut(s) 811
TseI GCWGC 4 cut(s) 387, 637, 881, 1100
Tsp45I GTSAC 1 cut(s) 811
TspDTI ATGAA 7 cut(s) 64, 66, 715, 748, 839, 905, 1059
TspRI CASTG 2 cut(s) 278, 526
VpaK11BI GGWCC 2 cut(s) 836, 1090
VspI ATTAAT 1 cut(s) 78
XapI RAATTY 4 cut(s) 70, 313, 722, 1066
XcmI CCANNNNNNNNNTGG 1 cut(s) 354
XmaJI CCTAGG 1 cut(s) 839
XmnI GAANNNNTTC 2 cut(s) 414, 726
XspI CTAG 3 cut(s) 254, 840, 1014
ZrmI AGTACT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.