FvH4_5g32040

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006657
Physical Location & Seq
Reverse (-)
23113483 .. 23115432
1950 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32040.t1

Sequence Viewer

Length: 1161 bp
ATGGAGGGCGTGACAGATTCTCCATGGGCTGTTGTGCCTTACCATGACCCTTATGATCACGAAGTCCCTGAAGTTCTGAGTTTTGTTAATGATTCTCCTGATCAAACTCTTGACGCATTGTTCGACGGTGGAGTAGTTCCTTCTTCCCCTCATCAATGTCCAGTTCCTCATTTTGATGATGGAATCCAAGATTTCCCCATGTCCCCAATGTGGGATTTCAGCAACAATTATAATGGGGGAGGGAATAATAATTTTTCAGTACCAGAGGATCCTCCTTCTCAGTGTTCCGGAGGAGGTGGAGAACCGGCTGTTGATCAGAATTCCGGGTTAGGGTTTGGGCAAAATGTGATGCCGCCTCCAGTTGTGGCGCCACCACCACCAATAATCAACTCCGGTTGCTGCGGCTGCCAGGCTCTAAGGGAAATCGTTCACTTCAGCCGCGACTATATCATGACACTTAAAATTCATGGAAGACTTGGTGCCATCTGCCATGCGATTCAAGAAAGCCGAGCCAATTACTTGTATGACTCCGGTGTTTCTAGCGAGCCTCACTATCAGAACTTAGACTTTTGCAATAAAGGCATTGAGGAAGTGAAGCAGTGTCTGACGCAGTACTGCTTAGAGCGAAAGGTAGAAGGCTACATCATGCAGCAAGATCCTCTGCAAGCATTTTATCAGACTCTCTGTGTTGGTATGGATGGTGAGTGGGATGATATTCTTTGTTATCCCGACGAATTTATTCCAGAGGACTTCGACGCCACCGACGACTTCATTTCTCCTAATTCAGAAAGGGTGGCAGAAATGGATCAACCTGCTACTGTGGCGACCGTGAATACGATAGGGAAGGGTCCTAGGCTTCCTAGGTATTCCCTTGCAGAACAGAGGGAGAGAGCAGCAAAAATGAAATTAGATGATCTTCGTGCTTACTTCCATCTCACCATTGAAGAGGCTTCTGAACAATTGCGTTTTTGCCCAACAGTTGTCAAGAGGATCTGTCGAAAGTTTGGAGTAAGCAGGTGGCCTGCTAGAAAGATCAAAAGCATTGAGAAGCGAATATCAAGCTTGAGGCCACTTTTGAATTCATGTAGTGAGAGCACCAGGGGCCATGTTGCAGCTGAAATCGAAAGGCTTGAAACAGAAGTAGCCCGACTTGTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

387

Amino Acids

43.05

Weight (kDa)

4.77

Isoelectric Point (pI)

59.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 300 - 348 2.3e-21 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 231
AarI CACCTGC 1 cut(s) 1005
Acc36I ACCTGC 2 cut(s) 820, 1005
AccB1I GGYRCC 2 cut(s) 367, 479
AccII CGCG 1 cut(s) 441
AccIII TCCGGA 1 cut(s) 287
AciI CCGC 3 cut(s) 353, 402, 439
AclWI GGATC 5 cut(s) 263, 276, 650, 813, 998
AcsI RAATTY 4 cut(s) 319, 462, 734, 1078
AcuI CTGAAG 2 cut(s) 90, 418
AcyI GRCGYC 2 cut(s) 368, 756
AfaI GTAC 3 cut(s) 261, 614, 1155
AfiI CCNNNNNNNGG 3 cut(s) 210, 211, 330
AgsI TTSAA 4 cut(s) 500, 944, 1078, 1133
AjnI CCWGG 2 cut(s) 408, 1097
AluBI AGCT 2 cut(s) 1062, 1115
AluI AGCT 2 cut(s) 1062, 1115
Alw21I GWGCWC 1 cut(s) 1097
AlwI GGATC 5 cut(s) 263, 276, 650, 813, 998
AlwNI CAGNNNCTG 1 cut(s) 604
Aor13HI TCCGGA 1 cut(s) 287
AoxI GGCC 3 cut(s) 1019, 1067, 1102
ApeKI GCWGC 5 cut(s) 399, 405, 649, 893, 1112
ApoI RAATTY 4 cut(s) 319, 462, 734, 1078
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
Asp700I GAANNNNTTC 2 cut(s) 426, 738
AspA2I CCTAGG 2 cut(s) 851, 860
AspLEI GCGC 1 cut(s) 370
AspS9I GGNCC 2 cut(s) 848, 1102
AsuC2I CCSGG 1 cut(s) 325
AsuHPI GGTGA 2 cut(s) 713, 928
AvaII GGWCC 1 cut(s) 848
AvrII CCTAGG 2 cut(s) 851, 860
BamHI GGATCC 1 cut(s) 268
BanI GGYRCC 2 cut(s) 367, 479
BbsI GAAGAC 1 cut(s) 478
Bbv12I GWGCWC 1 cut(s) 1097
BbvI GCAGC 5 cut(s) 386, 392, 661, 905, 1124
BccI CCATC 4 cut(s) 173, 491, 692, 939
BciT130I CCWGG 2 cut(s) 410, 1099
BclI TGATCA 3 cut(s) 55, 100, 313
BcnI CCSGG 1 cut(s) 325
BfaI CTAG 4 cut(s) 540, 852, 861, 1026
BfoI RGCGCY 1 cut(s) 371
BfuAI ACCTGC 2 cut(s) 820, 1005
BisI GCNGC 8 cut(s) 353, 400, 403, 406, 439, 650, 894, 1113
BlnI CCTAGG 2 cut(s) 851, 860
BlsI GCNGC 8 cut(s) 354, 401, 404, 407, 440, 651, 895, 1114
BmcAI AGTACT 1 cut(s) 614
Bme1390I CCNGG 3 cut(s) 325, 410, 1099
Bme18I GGWCC 1 cut(s) 848
BmgT120I GGNCC 2 cut(s) 848, 1102
BmiI GGNNCC 5 cut(s) 270, 369, 481, 849, 1103
BmrFI CCNGG 3 cut(s) 325, 410, 1099
BmsI GCATC 1 cut(s) 339
BpiI GAAGAC 1 cut(s) 478
BpmI CTGGAG 1 cut(s) 342
BpuEI CTTGAG 1 cut(s) 1084
BpuMI CCSGG 1 cut(s) 325
BsaHI GRCGYC 2 cut(s) 368, 756
BsaJI CCNNGG 4 cut(s) 23, 851, 860, 1098
BsaWI WCCGGW 3 cut(s) 287, 392, 530
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 211, 330
Bse118I RCCGGY 1 cut(s) 304
Bse1I ACTGG 2 cut(s) 161, 359
BseAI TCCGGA 1 cut(s) 287
BseBI CCWGG 2 cut(s) 410, 1099
BseDI CCNNGG 4 cut(s) 23, 851, 860, 1098
BseGI GGATG 2 cut(s) 703, 715
BseLI CCNNNNNNNGG 3 cut(s) 210, 211, 330
BseMII CTCAG 2 cut(s) 68, 293
BseNI ACTGG 2 cut(s) 161, 359
BseRI GAGGAG 1 cut(s) 306
BseXI GCAGC 5 cut(s) 386, 392, 661, 905, 1124
Bsh1236I CGCG 1 cut(s) 441
Bsh1285I CGRYCG 1 cut(s) 828
BshFI GGCC 3 cut(s) 1021, 1069, 1104
BshNI GGYRCC 2 cut(s) 367, 479
BsiEI CGRYCG 1 cut(s) 828
BsiHKAI GWGCWC 1 cut(s) 1097
BsiSI CCGG 5 cut(s) 288, 305, 324, 393, 531
BslFI GGGAC 2 cut(s) 50, 187
BslI CCNNNNNNNGG 3 cut(s) 210, 211, 330
BsmFI GGGAC 2 cut(s) 50, 187
BsnI GGCC 3 cut(s) 1021, 1069, 1104
Bsp1286I GDGCHC 1 cut(s) 1097
Bsp13I TCCGGA 1 cut(s) 287
Bsp143I GATC 9 cut(s) 55, 100, 268, 313, 655, 805, 913, 990, 1032
Bsp19I CCATGG 1 cut(s) 23
BspACI CCGC 3 cut(s) 353, 402, 439
BspANI GGCC 3 cut(s) 1021, 1069, 1104
BspCNI CTCAG 2 cut(s) 69, 292
BspEI TCCGGA 1 cut(s) 287
BspFNI CGCG 1 cut(s) 441
BspHI TCATGA 1 cut(s) 450
BspLI GGNNCC 5 cut(s) 270, 369, 481, 849, 1103
BspMI ACCTGC 2 cut(s) 820, 1005
BspPI GGATC 5 cut(s) 263, 276, 650, 813, 998
BspT107I GGYRCC 2 cut(s) 367, 479
BsrFI RCCGGY 1 cut(s) 304
BsrI ACTGG 2 cut(s) 161, 359
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 4 cut(s) 23, 851, 860, 1098
BssMI GATC 9 cut(s) 55, 100, 268, 313, 655, 805, 913, 990, 1032
BssNI GRCGYC 2 cut(s) 368, 756
BssT1I CCWWGG 3 cut(s) 23, 851, 860
Bst2UI CCWGG 2 cut(s) 410, 1099
Bst4CI ACNGT 4 cut(s) 128, 820, 829, 979
Bst6I CTCTTC 1 cut(s) 939
BstACI GRCGYC 2 cut(s) 368, 756
BstC8I GCNNGC 3 cut(s) 545, 666, 1023
BstDEI CTNAG 5 cut(s) 77, 279, 416, 562, 619
BstDSI CCRYGG 1 cut(s) 23
BstF5I GGATG 2 cut(s) 703, 715
BstFNI CGCG 1 cut(s) 441
BstH2I RGCGCY 1 cut(s) 371
BstHHI GCGC 1 cut(s) 370
BstKTI GATC 9 cut(s) 58, 103, 271, 316, 658, 808, 916, 993, 1035
BstMBI GATC 9 cut(s) 55, 100, 268, 313, 655, 805, 913, 990, 1032
BstMCI CGRYCG 1 cut(s) 828
BstMWI GCNNNNNNNGC 4 cut(s) 405, 579, 821, 1101
BstNI CCWGG 2 cut(s) 410, 1099
BstSCI CCNGG 3 cut(s) 323, 408, 1097
BstUI CGCG 1 cut(s) 441
BstV1I GCAGC 5 cut(s) 386, 392, 661, 905, 1124
BstV2I GAAGAC 1 cut(s) 478
BstX2I RGATCY 3 cut(s) 268, 655, 990
BstYI RGATCY 3 cut(s) 268, 655, 990
BsuRI GGCC 3 cut(s) 1021, 1069, 1104
BtgI CCRYGG 1 cut(s) 23
BtsCI GGATG 2 cut(s) 703, 715
BtsI GCAGTG 1 cut(s) 605
BtsIMutI CAGTG 2 cut(s) 287, 605
BveI ACCTGC 2 cut(s) 820, 1005
Cac8I GCNNGC 3 cut(s) 545, 666, 1023
CaiI CAGNNNCTG 1 cut(s) 604
CciI TCATGA 1 cut(s) 450
CfoI GCGC 1 cut(s) 370
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 2 cut(s) 848, 1102
CseI GACGC 3 cut(s) 122, 616, 764
Csp6I GTAC 3 cut(s) 260, 613, 1154
CviAII CATG 9 cut(s) 24, 44, 199, 451, 467, 491, 646, 1083, 1106
CviQI GTAC 3 cut(s) 260, 613, 1154
DdeI CTNAG 5 cut(s) 77, 279, 416, 562, 619
DinI GGCGCC 1 cut(s) 369
DpnI GATC 9 cut(s) 57, 102, 270, 315, 657, 807, 915, 992, 1034
DpnII GATC 9 cut(s) 55, 100, 268, 313, 655, 805, 913, 990, 1032
Eam1104I CTCTTC 1 cut(s) 939
EarI CTCTTC 1 cut(s) 939
Eco130I CCWWGG 3 cut(s) 23, 851, 860
Eco47I GGWCC 1 cut(s) 848
Eco57I CTGAAG 2 cut(s) 90, 418
EcoO109I RGGNCCY 1 cut(s) 848
EcoRI GAATTC 2 cut(s) 319, 1078
EcoRII CCWGG 2 cut(s) 408, 1097
EcoT14I CCWWGG 3 cut(s) 23, 851, 860
EgeI GGCGCC 1 cut(s) 369
EheI GGCGCC 1 cut(s) 369
ErhI CCWWGG 3 cut(s) 23, 851, 860
FaeI CATG 9 cut(s) 27, 47, 202, 454, 470, 494, 649, 1086, 1109
FaqI GGGAC 2 cut(s) 50, 187
FatI CATG 9 cut(s) 23, 43, 198, 450, 466, 490, 645, 1082, 1105
FbaI TGATCA 3 cut(s) 55, 100, 313
Fnu4HI GCNGC 8 cut(s) 353, 400, 403, 406, 439, 650, 894, 1113
FokI GGATG 2 cut(s) 710, 722
Fsp4HI GCNGC 8 cut(s) 353, 400, 403, 406, 439, 650, 894, 1113
FspBI CTAG 4 cut(s) 540, 852, 861, 1026
GlaI GCGC 1 cut(s) 369
GluI GCNGC 8 cut(s) 353, 400, 403, 406, 439, 650, 894, 1113
GsuI CTGGAG 1 cut(s) 342
HaeII RGCGCY 1 cut(s) 371
HaeIII GGCC 3 cut(s) 1021, 1069, 1104
HapII CCGG 5 cut(s) 288, 305, 324, 393, 531
HgaI GACGC 3 cut(s) 122, 616, 764
HhaI GCGC 1 cut(s) 370
Hin1I GRCGYC 2 cut(s) 368, 756
Hin1II CATG 9 cut(s) 27, 47, 202, 454, 470, 494, 649, 1086, 1109
Hin6I GCGC 1 cut(s) 368
HinP1I GCGC 1 cut(s) 368
HindIII AAGCTT 1 cut(s) 1060
HinfI GANTC 6 cut(s) 17, 92, 183, 496, 527, 679
HpaII CCGG 5 cut(s) 288, 305, 324, 393, 531
HphI GGTGA 2 cut(s) 713, 928
Hpy166II GTNNAC 1 cut(s) 430
Hpy188I TCNGA 7 cut(s) 78, 318, 558, 606, 678, 787, 955
Hpy188III TCNNGA 9 cut(s) 59, 98, 110, 288, 451, 500, 728, 743, 985
Hpy8I GTNNAC 1 cut(s) 430
Hpy99I CGWCG 4 cut(s) 128, 734, 758, 767
HpyAV CCTTC 4 cut(s) 150, 285, 629, 838
HpyCH4III ACNGT 4 cut(s) 128, 820, 829, 979
HpyCH4V TGCA 5 cut(s) 573, 649, 664, 875, 1112
HpyF10VI GCNNNNNNNGC 4 cut(s) 405, 579, 821, 1101
HpyF3I CTNAG 5 cut(s) 77, 279, 416, 562, 619
Hsp92I GRCGYC 2 cut(s) 368, 756
Hsp92II CATG 9 cut(s) 27, 47, 202, 454, 470, 494, 649, 1086, 1109
HspAI GCGC 1 cut(s) 368
KasI GGCGCC 1 cut(s) 367
Kpn2I TCCGGA 1 cut(s) 287
Ksp22I TGATCA 3 cut(s) 55, 100, 313
Kzo9I GATC 9 cut(s) 55, 100, 268, 313, 655, 805, 913, 990, 1032
Lsp1109I GCAGC 5 cut(s) 386, 392, 661, 905, 1124
LweI GCATC 1 cut(s) 339
MaeI CTAG 4 cut(s) 540, 852, 861, 1026
MaeIII GTNAC 1 cut(s) 10
MalI GATC 9 cut(s) 57, 102, 270, 315, 657, 807, 915, 992, 1034
MboI GATC 9 cut(s) 55, 100, 268, 313, 655, 805, 913, 990, 1032
MboII GAAGA 4 cut(s) 135, 483, 908, 956
MfeI CAATTG 1 cut(s) 959
MflI RGATCY 3 cut(s) 268, 655, 990
MhlI GDGCHC 1 cut(s) 1097
Mly113I GGCGCC 1 cut(s) 368
MlyI GAGTC 2 cut(s) 521, 673
MroI TCCGGA 1 cut(s) 287
MroXI GAANNNNTTC 2 cut(s) 426, 738
MseI TTAA 2 cut(s) 87, 459
MslI CAYNNNNRTG 1 cut(s) 174
MspA1I CMGCKG 1 cut(s) 1115
MspI CCGG 5 cut(s) 288, 305, 324, 393, 531
MspR9I CCNGG 3 cut(s) 325, 410, 1099
MunI CAATTG 1 cut(s) 959
MvaI CCWGG 2 cut(s) 410, 1099
MvnI CGCG 1 cut(s) 441
MwoI GCNNNNNNNGC 4 cut(s) 405, 579, 821, 1101
NarI GGCGCC 1 cut(s) 368
NciI CCSGG 1 cut(s) 325
NcoI CCATGG 1 cut(s) 23
NdeII GATC 9 cut(s) 55, 100, 268, 313, 655, 805, 913, 990, 1032
NlaIII CATG 9 cut(s) 27, 47, 202, 454, 470, 494, 649, 1086, 1109
NlaIV GGNNCC 5 cut(s) 270, 369, 481, 849, 1103
NmeAIII GCCGAG 1 cut(s) 533
NmuCI GTSAC 1 cut(s) 10
PagI TCATGA 1 cut(s) 450
PaqCI CACCTGC 1 cut(s) 1005
PcsI WCGNNNNNNNCGW 3 cut(s) 120, 759, 762
PdmI GAANNNNTTC 2 cut(s) 426, 738
PfeI GAWTC 4 cut(s) 17, 92, 183, 496
PkrI GCNGC 8 cut(s) 354, 401, 404, 407, 440, 651, 895, 1114
PleI GAGTC 2 cut(s) 521, 673
PluTI GGCGCC 1 cut(s) 371
PpsI GAGTC 2 cut(s) 521, 673
PpuMI RGGWCCY 1 cut(s) 848
PsiI TTATAA 1 cut(s) 231
Psp5II RGGWCCY 1 cut(s) 848
Psp6I CCWGG 2 cut(s) 408, 1097
PspGI CCWGG 2 cut(s) 408, 1097
PspN4I GGNNCC 5 cut(s) 270, 369, 481, 849, 1103
PspPI GGNCC 2 cut(s) 848, 1102
PspPPI RGGWCCY 1 cut(s) 848
PstNI CAGNNNCTG 1 cut(s) 604
PsuI RGATCY 3 cut(s) 268, 655, 990
PvuII CAGCTG 1 cut(s) 1115
RsaI GTAC 3 cut(s) 261, 614, 1155
RsaNI GTAC 3 cut(s) 260, 613, 1154
RseI CAYNNNNRTG 1 cut(s) 174
SaqAI TTAA 2 cut(s) 87, 459
SatI GCNGC 8 cut(s) 353, 400, 403, 406, 439, 650, 894, 1113
Sau3AI GATC 9 cut(s) 55, 100, 268, 313, 655, 805, 913, 990, 1032
Sau96I GGNCC 2 cut(s) 848, 1102
ScaI AGTACT 1 cut(s) 614
SchI GAGTC 2 cut(s) 521, 673
ScrFI CCNGG 3 cut(s) 325, 410, 1099
SduI GDGCHC 1 cut(s) 1097
SetI ASST 7 cut(s) 298, 633, 814, 866, 1019, 1064, 1117
SfaNI GCATC 1 cut(s) 339
SfoI GGCGCC 1 cut(s) 369
SinI GGWCC 1 cut(s) 848
SmiMI CAYNNNNRTG 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 1063
SmoI CTYRAG 1 cut(s) 1063
SsiI CCGC 3 cut(s) 353, 402, 439
SspDI GGCGCC 1 cut(s) 367
SspMI CTAG 4 cut(s) 540, 852, 861, 1026
StyD4I CCNGG 3 cut(s) 323, 408, 1097
StyI CCWWGG 3 cut(s) 23, 851, 860
TaaI ACNGT 4 cut(s) 128, 820, 829, 979
TaqI TCGA 4 cut(s) 123, 753, 997, 1122
TatI WGTACW 1 cut(s) 612
TauI GCSGC 3 cut(s) 355, 405, 441
TfiI GAWTC 4 cut(s) 17, 92, 183, 496
Tru1I TTAA 2 cut(s) 87, 459
Tru9I TTAA 2 cut(s) 87, 459
TscAI CASTG 2 cut(s) 287, 605
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 5 cut(s) 399, 405, 649, 893, 1112
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 4 cut(s) 455, 760, 917, 1071
TspRI CASTG 2 cut(s) 287, 605
VpaK11BI GGWCC 1 cut(s) 848
XapI RAATTY 4 cut(s) 319, 462, 734, 1078
XmaJI CCTAGG 2 cut(s) 851, 860
XmnI GAANNNNTTC 2 cut(s) 426, 738
XspI CTAG 4 cut(s) 540, 852, 861, 1026
ZrmI AGTACT 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.