Rroxscaffold_3G00231600

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006657
Physical Location & Seq
Forward (+)
16860878 .. 16862442
1565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00231600.1

Sequence Viewer

Length: 1149 bp
ATGACAGAGTCTCCATGGGCTGTTGTGCCTTACCATGACCCTTATGATCATGAAGTCCCCGACGTTGTGAATTTCATTAATGATACTCCTGATCCAACCCTTGACACATTGTTCGACGGTGGAGTAGTTCCTTCTTTCTCTCATCAATATCCAATTCCTCAGTTTGATAATGGAATCCAAGATTTTCCCATGTCCCCAATGTGGGATTTGGGCAACAATTATAATGGTGGAGGGAATAATACTATTTCAGTACTAGCGGATCATCCTTCGCAGTGTTCCGGAGGAGGAGGAGGAGAATCGGGTTTTGATCAGAATTCGGGGTTAGGGTTTGGGAGTAATGTGATGCCACCTCCAGTTTGGGCTCCACCACCAATCCCCTTCAGTTGCTGTGGTTGCCAAGTTCTAAGGGAAATTGTTCACTTCAATCGCAATTATATCATGACACTTGAAATCCATGGAAGACTCGGTGCCATCTGTCATGCAATTCAAGAAAGACGCGCCAATTACTTGTACGACTCCAGTGCTTCTATTGAGCCCCACTATCAGAACTTTGACTTTTGCAATAAAAACATAGAGGAAGTGAAGCAGTTTCTGACGCAGTACTGCTTAGAGCGAAAGGTAGAAGGCTACATTATGCAGCAAGATCCTCTGCAAGCATTTTATCAGACTCTCTGTGTTGGTATGGATGGTGAGTGGGATGATATTCTTAGTTATCCCGACGAATTCATTCCTGAGGACTTGGACACTACCGACGACTTCATTTCTCCTAATTCAGAAGGGGCGGAAGAAATGGAACAACCTATCGATGAGGTGACCGTGAATATGAAAAGGAAGGGTCCTAGGCTTCCCAGGTTTTCCCTTGCAGAACAGAGGGAGAGAGCAGCAAAAATGAAATTAAATGATCTTCGTGCTTACTTCCATCTCACCATTGAAGAGGCTTCTGAGCAATTGGGTTTTTGCCCAACTGTTGTCAAGAGGATCTGCCGCAAGTTTGGAGTAAGCCGGTGGCCTGCTAGAAAGATCAAAAGCATTGAGAGGCGAATATCAAGCTTGAGGCCGCTTTTGAATTCATATAGTGACAGCACCAGGGTCCACGTTGTAGCTGAAATTAAAAGGCTTGAAAGAGAAGTATCCCGACTTGTATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

382

Amino Acids

43.35

Weight (kDa)

4.94

Isoelectric Point (pI)

55.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 296 - 344 9.9e-22 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 222
AccB1I GGYRCC 1 cut(s) 467
AccII CGCG 1 cut(s) 498
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 4 cut(s) 257, 782, 985, 1058
AclWI GGATC 4 cut(s) 86, 267, 638, 986
AcsI RAATTY 4 cut(s) 70, 313, 722, 1066
AcuI CTGAAG 1 cut(s) 364
AfaI GTAC 3 cut(s) 252, 512, 602
AfiI CCNNNNNNNGG 2 cut(s) 201, 202
AgsI TTSAA 6 cut(s) 424, 449, 488, 932, 1066, 1121
AjnI CCWGG 2 cut(s) 848, 1085
AluBI AGCT 2 cut(s) 1050, 1103
AluI AGCT 2 cut(s) 1050, 1103
Alw26I GTCTC 1 cut(s) 15
AlwI GGATC 4 cut(s) 86, 267, 638, 986
AlwNI CAGNNNCTG 2 cut(s) 387, 592
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 2 cut(s) 1007, 1055
ApeKI GCWGC 2 cut(s) 637, 881
ApoI RAATTY 4 cut(s) 70, 313, 722, 1066
ArsI GACNNNNNNTTYG 2 cut(s) 95, 127
AseI ATTAAT 1 cut(s) 78
Asp700I GAANNNNTTC 2 cut(s) 414, 726
AspA2I CCTAGG 1 cut(s) 839
AspLEI GCGC 1 cut(s) 500
AspS9I GGNCC 2 cut(s) 836, 1090
AsuHPI GGTGA 3 cut(s) 701, 823, 916
AvaII GGWCC 2 cut(s) 836, 1090
AvrII CCTAGG 1 cut(s) 839
AxyI CCTNAGG 1 cut(s) 732
BanI GGYRCC 1 cut(s) 467
BanII GRGCYC 2 cut(s) 364, 537
BbsI GAAGAC 1 cut(s) 466
BbvI GCAGC 2 cut(s) 649, 893
BccI CCATC 3 cut(s) 479, 680, 927
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BciT130I CCWGG 2 cut(s) 850, 1087
BciVI GTATCC 1 cut(s) 1141
BclI TGATCA 2 cut(s) 46, 307
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 3 cut(s) 254, 840, 1014
BfuI GTATCC 1 cut(s) 1141
BisI GCNGC 4 cut(s) 638, 882, 985, 1058
BlnI CCTAGG 1 cut(s) 839
BlsI GCNGC 4 cut(s) 639, 883, 986, 1059
BmcAI AGTACT 2 cut(s) 252, 602
Bme1390I CCNGG 2 cut(s) 850, 1087
Bme18I GGWCC 2 cut(s) 836, 1090
BmgT120I GGNCC 2 cut(s) 836, 1090
BmiI GGNNCC 4 cut(s) 363, 469, 837, 1091
BmrFI CCNGG 2 cut(s) 850, 1087
BmsI GCATC 1 cut(s) 333
BpiI GAAGAC 1 cut(s) 466
BpmI CTGGAG 2 cut(s) 336, 502
BpuEI CTTGAG 1 cut(s) 1072
Bsa29I ATCGAT 1 cut(s) 804
BsaJI CCNNGG 5 cut(s) 14, 454, 839, 848, 1086
BsaWI WCCGGW 1 cut(s) 278
BsaXI ACNNNNNCTCC 1 cut(s) 25
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 202
Bse118I RCCGGY 1 cut(s) 1002
Bse1I ACTGG 2 cut(s) 353, 519
Bse21I CCTNAGG 1 cut(s) 732
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 2 cut(s) 850, 1087
BseCI ATCGAT 1 cut(s) 804
BseDI CCNNGG 5 cut(s) 14, 454, 839, 848, 1086
BseGI GGATG 3 cut(s) 262, 691, 703
BseLI CCNNNNNNNGG 2 cut(s) 201, 202
BseMII CTCAG 3 cut(s) 173, 723, 933
BseNI ACTGG 2 cut(s) 353, 519
BseRI GAGGAG 4 cut(s) 297, 300, 303, 306
BseXI GCAGC 2 cut(s) 649, 893
Bsh1236I CGCG 1 cut(s) 498
BshFI GGCC 2 cut(s) 1009, 1057
BshNI GGYRCC 1 cut(s) 467
BshVI ATCGAT 1 cut(s) 804
BsiSI CCGG 2 cut(s) 279, 1003
BslFI GGGAC 2 cut(s) 41, 178
BslI CCNNNNNNNGG 2 cut(s) 201, 202
BsmAI GTCTC 1 cut(s) 15
BsmFI GGGAC 2 cut(s) 41, 178
BsnI GGCC 2 cut(s) 1009, 1057
Bsp1286I GDGCHC 2 cut(s) 364, 537
Bsp13I TCCGGA 1 cut(s) 278
Bsp143I GATC 8 cut(s) 46, 91, 259, 307, 643, 901, 978, 1020
Bsp19I CCATGG 2 cut(s) 14, 454
BspACI CCGC 4 cut(s) 257, 782, 985, 1058
BspANI GGCC 2 cut(s) 1009, 1057
BspCNI CTCAG 3 cut(s) 172, 724, 934
BspDI ATCGAT 1 cut(s) 804
BspEI TCCGGA 1 cut(s) 278
BspFNI CGCG 1 cut(s) 498
BspHI TCATGA 2 cut(s) 49, 438
BspLI GGNNCC 4 cut(s) 363, 469, 837, 1091
BspPI GGATC 4 cut(s) 86, 267, 638, 986
BspT107I GGYRCC 1 cut(s) 467
BsrFI RCCGGY 1 cut(s) 1002
BsrI ACTGG 2 cut(s) 353, 519
BssAI RCCGGY 1 cut(s) 1002
BssECI CCNNGG 5 cut(s) 14, 454, 839, 848, 1086
BssMI GATC 8 cut(s) 46, 91, 259, 307, 643, 901, 978, 1020
BssT1I CCWWGG 3 cut(s) 14, 454, 839
Bst2UI CCWGG 2 cut(s) 850, 1087
Bst4CI ACNGT 3 cut(s) 119, 817, 967
Bst6I CTCTTC 1 cut(s) 927
BstC8I GCNNGC 2 cut(s) 654, 1011
BstDEI CTNAG 6 cut(s) 159, 404, 607, 707, 732, 942
BstDSI CCRYGG 2 cut(s) 14, 454
BstEII GGTNACC 1 cut(s) 811
BstF5I GGATG 3 cut(s) 262, 691, 703
BstFNI CGCG 1 cut(s) 498
BstHHI GCGC 1 cut(s) 500
BstKTI GATC 8 cut(s) 49, 94, 262, 310, 646, 904, 981, 1023
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 8 cut(s) 46, 91, 259, 307, 643, 901, 978, 1020
BstMWI GCNNNNNNNGC 1 cut(s) 393
BstNI CCWGG 2 cut(s) 850, 1087
BstPI GGTNACC 1 cut(s) 811
BstSCI CCNGG 2 cut(s) 848, 1085
BstUI CGCG 1 cut(s) 498
BstV1I GCAGC 2 cut(s) 649, 893
BstV2I GAAGAC 1 cut(s) 466
BstX2I RGATCY 2 cut(s) 643, 978
BstYI RGATCY 2 cut(s) 643, 978
Bsu15I ATCGAT 1 cut(s) 804
Bsu36I CCTNAGG 1 cut(s) 732
BsuI GTATCC 1 cut(s) 1141
BsuRI GGCC 2 cut(s) 1009, 1057
BsuTUI ATCGAT 1 cut(s) 804
BtgI CCRYGG 2 cut(s) 14, 454
BtsCI GGATG 3 cut(s) 262, 691, 703
BtsI GCAGTG 1 cut(s) 278
BtsIMutI CAGTG 2 cut(s) 278, 526
Cac8I GCNNGC 2 cut(s) 654, 1011
CaiI CAGNNNCTG 2 cut(s) 387, 592
CciI TCATGA 2 cut(s) 49, 438
CfoI GCGC 1 cut(s) 500
Cfr10I RCCGGY 1 cut(s) 1002
Cfr13I GGNCC 2 cut(s) 836, 1090
ClaI ATCGAT 1 cut(s) 804
CseI GACGC 2 cut(s) 504, 604
Csp6I GTAC 3 cut(s) 251, 511, 601
CviAII CATG 7 cut(s) 15, 35, 50, 190, 439, 455, 479
CviQI GTAC 3 cut(s) 251, 511, 601
DdeI CTNAG 6 cut(s) 159, 404, 607, 707, 732, 942
DpnI GATC 8 cut(s) 48, 93, 261, 309, 645, 903, 980, 1022
DpnII GATC 8 cut(s) 46, 91, 259, 307, 643, 901, 978, 1020
Eam1104I CTCTTC 1 cut(s) 927
EarI CTCTTC 1 cut(s) 927
EciI GGCGGA 1 cut(s) 797
Eco130I CCWWGG 3 cut(s) 14, 454, 839
Eco24I GRGCYC 2 cut(s) 364, 537
Eco47I GGWCC 2 cut(s) 836, 1090
Eco57I CTGAAG 1 cut(s) 364
Eco81I CCTNAGG 1 cut(s) 732
Eco91I GGTNACC 1 cut(s) 811
EcoO109I RGGNCCY 1 cut(s) 836
EcoO65I GGTNACC 1 cut(s) 811
EcoRI GAATTC 3 cut(s) 313, 722, 1066
EcoRII CCWGG 2 cut(s) 848, 1085
EcoT14I CCWWGG 3 cut(s) 14, 454, 839
EcoT38I GRGCYC 2 cut(s) 364, 537
ErhI CCWWGG 3 cut(s) 14, 454, 839
FaeI CATG 7 cut(s) 18, 38, 53, 193, 442, 458, 482
FaqI GGGAC 2 cut(s) 41, 178
FatI CATG 7 cut(s) 14, 34, 49, 189, 438, 454, 478
FbaI TGATCA 2 cut(s) 46, 307
Fnu4HI GCNGC 4 cut(s) 638, 882, 985, 1058
FokI GGATG 3 cut(s) 249, 698, 710
FriOI GRGCYC 2 cut(s) 364, 537
Fsp4HI GCNGC 4 cut(s) 638, 882, 985, 1058
FspBI CTAG 3 cut(s) 254, 840, 1014
GlaI GCGC 1 cut(s) 499
GluI GCNGC 4 cut(s) 638, 882, 985, 1058
GsuI CTGGAG 2 cut(s) 336, 502
HaeIII GGCC 2 cut(s) 1009, 1057
HapII CCGG 2 cut(s) 279, 1003
HgaI GACGC 2 cut(s) 504, 604
HhaI GCGC 1 cut(s) 500
Hin1II CATG 7 cut(s) 18, 38, 53, 193, 442, 458, 482
Hin6I GCGC 1 cut(s) 498
HinP1I GCGC 1 cut(s) 498
HindIII AAGCTT 1 cut(s) 1048
HinfI GANTC 6 cut(s) 8, 174, 296, 462, 515, 667
HpaII CCGG 2 cut(s) 279, 1003
HphI GGTGA 3 cut(s) 701, 823, 916
Hpy166II GTNNAC 2 cut(s) 418, 1093
Hpy188I TCNGA 6 cut(s) 312, 546, 594, 666, 775, 943
Hpy188III TCNNGA 9 cut(s) 50, 89, 279, 439, 488, 716, 731, 973, 1134
Hpy8I GTNNAC 2 cut(s) 418, 1093
Hpy99I CGWCG 4 cut(s) 65, 119, 722, 755
HpyAV CCTTC 6 cut(s) 141, 276, 388, 617, 770, 826
HpyCH4III ACNGT 3 cut(s) 119, 817, 967
HpyCH4IV ACGT 2 cut(s) 63, 1095
HpyCH4V TGCA 5 cut(s) 482, 561, 637, 652, 863
HpyF10VI GCNNNNNNNGC 1 cut(s) 393
HpyF3I CTNAG 6 cut(s) 159, 404, 607, 707, 732, 942
HpySE526I ACGT 2 cut(s) 63, 1095
Hsp92II CATG 7 cut(s) 18, 38, 53, 193, 442, 458, 482
HspAI GCGC 1 cut(s) 498
Kpn2I TCCGGA 1 cut(s) 278
Ksp22I TGATCA 2 cut(s) 46, 307
Kzo9I GATC 8 cut(s) 46, 91, 259, 307, 643, 901, 978, 1020
LmnI GCTCC 1 cut(s) 367
Lsp1109I GCAGC 2 cut(s) 649, 893
LweI GCATC 1 cut(s) 333
MaeI CTAG 3 cut(s) 254, 840, 1014
MaeII ACGT 2 cut(s) 63, 1095
MaeIII GTNAC 2 cut(s) 811, 1076
MalI GATC 8 cut(s) 48, 93, 261, 309, 645, 903, 980, 1022
MboI GATC 8 cut(s) 46, 91, 259, 307, 643, 901, 978, 1020
MboII GAAGA 4 cut(s) 471, 797, 896, 944
MfeI CAATTG 1 cut(s) 947
MflI RGATCY 2 cut(s) 643, 978
MhlI GDGCHC 2 cut(s) 364, 537
MlyI GAGTC 4 cut(s) 17, 456, 509, 661
MmeI TCCRAC 1 cut(s) 119
MroI TCCGGA 1 cut(s) 278
MroXI GAANNNNTTC 2 cut(s) 414, 726
MseI TTAA 3 cut(s) 78, 896, 1110
MspI CCGG 2 cut(s) 279, 1003
MspR9I CCNGG 2 cut(s) 850, 1087
MunI CAATTG 1 cut(s) 947
MvaI CCWGG 2 cut(s) 850, 1087
MvnI CGCG 1 cut(s) 498
MwoI GCNNNNNNNGC 1 cut(s) 393
NcoI CCATGG 2 cut(s) 14, 454
NdeII GATC 8 cut(s) 46, 91, 259, 307, 643, 901, 978, 1020
NlaIII CATG 7 cut(s) 18, 38, 53, 193, 442, 458, 482
NlaIV GGNNCC 4 cut(s) 363, 469, 837, 1091
NmuCI GTSAC 2 cut(s) 811, 1076
PagI TCATGA 2 cut(s) 49, 438
PdmI GAANNNNTTC 2 cut(s) 414, 726
PfeI GAWTC 2 cut(s) 174, 296
PflFI GACNNNGTC 1 cut(s) 7
PkrI GCNGC 4 cut(s) 639, 883, 986, 1059
PleI GAGTC 4 cut(s) 16, 456, 509, 661
PpsI GAGTC 4 cut(s) 16, 456, 509, 661
PpuMI RGGWCCY 1 cut(s) 836
PshBI ATTAAT 1 cut(s) 78
PsiI TTATAA 1 cut(s) 222
Psp5II RGGWCCY 1 cut(s) 836
Psp6I CCWGG 2 cut(s) 848, 1085
PspEI GGTNACC 1 cut(s) 811
PspGI CCWGG 2 cut(s) 848, 1085
PspN4I GGNNCC 4 cut(s) 363, 469, 837, 1091
PspPI GGNCC 2 cut(s) 836, 1090
PspPPI RGGWCCY 1 cut(s) 836
PstNI CAGNNNCTG 2 cut(s) 387, 592
PsuI RGATCY 2 cut(s) 643, 978
PsyI GACNNNGTC 1 cut(s) 7
RsaI GTAC 3 cut(s) 252, 512, 602
RsaNI GTAC 3 cut(s) 251, 511, 601
SaqAI TTAA 3 cut(s) 78, 896, 1110
SatI GCNGC 4 cut(s) 638, 882, 985, 1058
Sau3AI GATC 8 cut(s) 46, 91, 259, 307, 643, 901, 978, 1020
Sau96I GGNCC 2 cut(s) 836, 1090
ScaI AGTACT 2 cut(s) 252, 602
SchI GAGTC 4 cut(s) 17, 456, 509, 661
ScrFI CCNGG 2 cut(s) 850, 1087
SduI GDGCHC 2 cut(s) 364, 537
SetI ASST 9 cut(s) 66, 352, 621, 802, 813, 854, 1052, 1098, 1105
SfaNI GCATC 1 cut(s) 333
SinI GGWCC 2 cut(s) 836, 1090
SmlI CTYRAG 1 cut(s) 1051
SmoI CTYRAG 1 cut(s) 1051
SsiI CCGC 4 cut(s) 257, 782, 985, 1058
SspMI CTAG 3 cut(s) 254, 840, 1014
StyD4I CCNGG 2 cut(s) 848, 1085
StyI CCWWGG 3 cut(s) 14, 454, 839
TaaI ACNGT 3 cut(s) 119, 817, 967
TaiI ACGT 2 cut(s) 66, 1098
TaqI TCGA 2 cut(s) 114, 804
TatI WGTACW 2 cut(s) 250, 600
TauI GCSGC 2 cut(s) 987, 1060
TfiI GAWTC 2 cut(s) 174, 296
Tru1I TTAA 3 cut(s) 78, 896, 1110
Tru9I TTAA 3 cut(s) 78, 896, 1110
TscAI CASTG 2 cut(s) 278, 526
TseFI GTSAC 2 cut(s) 811, 1076
TseI GCWGC 2 cut(s) 637, 881
Tsp45I GTSAC 2 cut(s) 811, 1076
TspDTI ATGAA 7 cut(s) 64, 66, 715, 748, 839, 905, 1059
TspRI CASTG 2 cut(s) 278, 526
Tth111I GACNNNGTC 1 cut(s) 7
VpaK11BI GGWCC 2 cut(s) 836, 1090
VspI ATTAAT 1 cut(s) 78
XapI RAATTY 4 cut(s) 70, 313, 722, 1066
XcmI CCANNNNNNNNNTGG 1 cut(s) 354
XmaJI CCTAGG 1 cut(s) 839
XmnI GAANNNNTTC 2 cut(s) 414, 726
XspI CTAG 3 cut(s) 254, 840, 1014
ZrmI AGTACT 2 cut(s) 252, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.