FvH4_7g07010

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006657
Physical Location & Seq
Forward (+)
6984055 .. 6985849
1795 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g07010.t1

Sequence Viewer

Length: 363 bp
ATGGAACAATCTGCGGAGGAGGCGTCTCCGAAAGGGAATGGTCCTAGGCTTCCTAGGTATTCCCTTGCAGAACAGAGGGAGAGAGCAGCAAAAATGAAATTAGACGATCTTCGTGCTTACTTCCGTCTCACCATTGAAGAGGCTTCTGAGCAATTGCGTTTTTGCCCAACTGTTGTCAAGAGGATCTGCCGCAAGTTTGGAGTAAGCAGGTGGCCTGCTAGAAAGATCAAAAGCATTGAGAAACAAATATCAAGCTTGAGGCCACTTTTGTATTCATGTAGTGAGAGCACCAGGGTCCATGTCGCAGCTAAAATCAAAAGGTTTGAAAGAGAAGTGGCTCGATTTGTACCATATGTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

121

Amino Acids

13.99

Weight (kDa)

10.01

Isoelectric Point (pI)

63.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 31 - 79 3.5e-21 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 198
Acc36I ACCTGC 1 cut(s) 198
AciI CCGC 2 cut(s) 14, 190
AclWI GGATC 1 cut(s) 191
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 1 cut(s) 348
AgsI TTSAA 2 cut(s) 137, 326
AjnI CCWGG 1 cut(s) 290
AluBI AGCT 2 cut(s) 255, 308
AluI AGCT 2 cut(s) 255, 308
Alw21I GWGCWC 1 cut(s) 290
Alw26I GTCTC 2 cut(s) 30, 131
AlwI GGATC 1 cut(s) 191
AoxI GGCC 2 cut(s) 212, 260
ApeKI GCWGC 2 cut(s) 86, 305
AspA2I CCTAGG 2 cut(s) 44, 53
AspS9I GGNCC 2 cut(s) 41, 295
AsuHPI GGTGA 1 cut(s) 121
AvaII GGWCC 2 cut(s) 41, 295
AvrII CCTAGG 2 cut(s) 44, 53
Bbv12I GWGCWC 1 cut(s) 290
BbvI GCAGC 2 cut(s) 98, 317
BcgI CGANNNNNNTGC 2 cut(s) 95, 129
BciT130I CCWGG 1 cut(s) 292
BcoDI GTCTC 2 cut(s) 30, 131
BfaI CTAG 3 cut(s) 45, 54, 219
BfuAI ACCTGC 1 cut(s) 198
BisI GCNGC 3 cut(s) 87, 190, 306
BlnI CCTAGG 2 cut(s) 44, 53
BlsI GCNGC 3 cut(s) 88, 191, 307
Bme1390I CCNGG 1 cut(s) 292
Bme18I GGWCC 2 cut(s) 41, 295
BmgT120I GGNCC 2 cut(s) 41, 295
BmiI GGNNCC 1 cut(s) 296
BmrFI CCNGG 1 cut(s) 292
BpuEI CTTGAG 1 cut(s) 277
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 3 cut(s) 44, 53, 291
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
BseBI CCWGG 1 cut(s) 292
BseDI CCNNGG 3 cut(s) 44, 53, 291
BseMII CTCAG 1 cut(s) 138
BseRI GAGGAG 1 cut(s) 32
BseXI GCAGC 2 cut(s) 98, 317
BshFI GGCC 2 cut(s) 214, 262
BsiHKAI GWGCWC 1 cut(s) 290
BsmAI GTCTC 2 cut(s) 30, 131
BsmBI CGTCTC 2 cut(s) 30, 131
BsnI GGCC 2 cut(s) 214, 262
Bsp1286I GDGCHC 1 cut(s) 290
Bsp143I GATC 3 cut(s) 106, 183, 225
BspACI CCGC 2 cut(s) 14, 190
BspANI GGCC 2 cut(s) 214, 262
BspCNI CTCAG 1 cut(s) 139
BspLI GGNNCC 1 cut(s) 296
BspMI ACCTGC 1 cut(s) 198
BspPI GGATC 1 cut(s) 191
BssECI CCNNGG 3 cut(s) 44, 53, 291
BssMI GATC 3 cut(s) 106, 183, 225
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 2 cut(s) 44, 53
Bst2UI CCWGG 1 cut(s) 292
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 1 cut(s) 132
BstACI GRCGYC 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 1 cut(s) 147
BstKTI GATC 3 cut(s) 109, 186, 228
BstMAI GTCTC 2 cut(s) 30, 131
BstMBI GATC 3 cut(s) 106, 183, 225
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstNI CCWGG 1 cut(s) 292
BstSCI CCNGG 1 cut(s) 290
BstV1I GCAGC 2 cut(s) 98, 317
BstX2I RGATCY 1 cut(s) 183
BstYI RGATCY 1 cut(s) 183
BsuRI GGCC 2 cut(s) 214, 262
BveI ACCTGC 1 cut(s) 198
Cac8I GCNNGC 1 cut(s) 216
Cfr13I GGNCC 2 cut(s) 41, 295
CseI GACGC 1 cut(s) 12
Csp6I GTAC 1 cut(s) 347
CviAII CATG 2 cut(s) 276, 299
CviJI RGCY 7 cut(s) 49, 143, 214, 255, 262, 308, 338
CviKI_1 RGCY 7 cut(s) 49, 143, 214, 255, 262, 308, 338
CviQI GTAC 1 cut(s) 347
DdeI CTNAG 1 cut(s) 147
DpnI GATC 3 cut(s) 108, 185, 227
DpnII GATC 3 cut(s) 106, 183, 225
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Eco130I CCWWGG 2 cut(s) 44, 53
Eco47I GGWCC 2 cut(s) 41, 295
EcoRII CCWGG 1 cut(s) 290
EcoT14I CCWWGG 2 cut(s) 44, 53
ErhI CCWWGG 2 cut(s) 44, 53
Esp3I CGTCTC 2 cut(s) 30, 131
FaeI CATG 2 cut(s) 279, 302
FaiI YATR 4 cut(s) 277, 300, 352, 354
FatI CATG 2 cut(s) 275, 298
FauNDI CATATG 1 cut(s) 352
Fnu4HI GCNGC 3 cut(s) 87, 190, 306
Fsp4HI GCNGC 3 cut(s) 87, 190, 306
FspBI CTAG 3 cut(s) 45, 54, 219
GluI GCNGC 3 cut(s) 87, 190, 306
HaeIII GGCC 2 cut(s) 214, 262
HgaI GACGC 1 cut(s) 12
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 2 cut(s) 279, 302
HindIII AAGCTT 1 cut(s) 253
HphI GGTGA 1 cut(s) 121
Hpy188I TCNGA 2 cut(s) 30, 148
Hpy188III TCNNGA 1 cut(s) 178
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4V TGCA 1 cut(s) 68
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpyF3I CTNAG 1 cut(s) 147
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 2 cut(s) 279, 302
Kzo9I GATC 3 cut(s) 106, 183, 225
LpnPI CCDG 4 cut(s) 193, 228, 277, 304
Lsp1109I GCAGC 2 cut(s) 98, 317
MaeI CTAG 3 cut(s) 45, 54, 219
MalI GATC 3 cut(s) 108, 185, 227
MboI GATC 3 cut(s) 106, 183, 225
MboII GAAGA 2 cut(s) 101, 149
MfeI CAATTG 1 cut(s) 152
MflI RGATCY 1 cut(s) 183
MhlI GDGCHC 1 cut(s) 290
MluCI AATT 2 cut(s) 98, 152
MnlI CCTC 6 cut(s) 10, 13, 69, 133, 174, 252
MspR9I CCNGG 1 cut(s) 292
MunI CAATTG 1 cut(s) 152
MvaI CCWGG 1 cut(s) 292
MwoI GCNNNNNNNGC 1 cut(s) 20
NdeI CATATG 1 cut(s) 352
NdeII GATC 3 cut(s) 106, 183, 225
NlaIII CATG 2 cut(s) 279, 302
NlaIV GGNNCC 1 cut(s) 296
PaqCI CACCTGC 1 cut(s) 198
PkrI GCNGC 3 cut(s) 88, 191, 307
Psp6I CCWGG 1 cut(s) 290
PspGI CCWGG 1 cut(s) 290
PspN4I GGNNCC 1 cut(s) 296
PspPI GGNCC 2 cut(s) 41, 295
PsuI RGATCY 1 cut(s) 183
RsaI GTAC 1 cut(s) 348
RsaNI GTAC 1 cut(s) 347
SatI GCNGC 3 cut(s) 87, 190, 306
Sau3AI GATC 3 cut(s) 106, 183, 225
Sau96I GGNCC 2 cut(s) 41, 295
ScrFI CCNGG 1 cut(s) 292
SduI GDGCHC 1 cut(s) 290
SetI ASST 5 cut(s) 59, 212, 257, 310, 323
SinI GGWCC 2 cut(s) 41, 295
SmlI CTYRAG 1 cut(s) 256
SmoI CTYRAG 1 cut(s) 256
Sse9I AATT 2 cut(s) 98, 152
SsiI CCGC 2 cut(s) 14, 190
SspMI CTAG 3 cut(s) 45, 54, 219
StyD4I CCNGG 1 cut(s) 290
StyI CCWWGG 2 cut(s) 44, 53
TaaI ACNGT 1 cut(s) 172
TaqI TCGA 1 cut(s) 340
TasI AATT 2 cut(s) 98, 152
TauI GCSGC 1 cut(s) 192
TseI GCWGC 2 cut(s) 86, 305
TspDTI ATGAA 2 cut(s) 110, 264
TspGWI ACGGA 1 cut(s) 113
VpaK11BI GGWCC 2 cut(s) 41, 295
XmaJI CCTAGG 2 cut(s) 44, 53
XspI CTAG 3 cut(s) 45, 54, 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.