Rroxscaffold_4G00310200

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006657
Physical Location & Seq
Reverse (-)
32106992 .. 32110048
3057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00310200.1

Sequence Viewer

Length: 1215 bp
ATGGCAGATATTCCATGGGATGTTTTGCCGTACCATGACCCTTACAATCAAGAAGTCCCTGACATTCTGAGTTCCGAGAATGATTCTTTTGATCTAAGCCTTCAGAACATCTCGGCTCTGTTGGACGTAGTAGTACCTCCATCCCCTCATTCTCATGCAGTTCCTGTTTTTGATAATGACATCCAAAACATCAACAATCCCATTCCCTTTAATGCCCCAGTACGGAATTTTGGCAACAATGCTAGTCTGTCCAGTAATGGAGGAGGAGGAGGGAGTCCGACTATTTCACTGTCGGATCATTTCTCAGAGATTTTTGGGGCAGAAGCTAATACTATTTCACTACCAGATCTTCCCTCGCAGATTTTCGGAGCAGGAGGCAATAACATTTTACTAGCGGATATTCCCTCGCATATCTTCGAAACAGGAGGCAATATTGTTTCACTACCGGATCTTTCGTCGCAGAATTTCAGAGCAAGAGGCAATACAAACAAGTATATCATCAAATTTGAAATTCATGGAAAACGCGGCGTCATCTGTCATGCAATTCAAGAACGTCGCGCCAATGTCTTCCACAATTCCGGTGCTTCCAGTGAACCTCTCTATGAGAGCTTCGATTTTCGCACTAAAAGCCTAAAGGAAGTGAAGCACTTTCTAACGCGGTATTGCGAGGAGCGAAAGGCCGAAGGCTATATAAAGCAATTGGATCCTATGCAGCCATTTTTTCAGCCTCCCTTTGTCGGAATGGATGAAGATGAGTGGGAAGATGTTCTAACCTATCCTGAAGAATACATTCCCGAGGACTTGGATATTGACGACTTCGATCTACCAAATTTAGATGAAATGGAGCGCCAACCTGCTGATAAGGCGTCGTCCGGGAATTGGAAACGGAAGGGTCCTGCTAAGCGTCCTAGGCTTTCCCTTCCAGAACAGAGGGAGAGAGCGGCGAAGATGAAATTGGAGGATCTCCGTGTTTACTTCCATCTCACCTTTGAAGAGGCTTCTGAGGAATTGCGATTATCCGCAGCTGTTGTCAAAAGGCTCTGCCGCAAGTTTGGAATAGGCAGATGGCCTGGTAGAAAGATCAAAGCCATTTCAAAGATGATTTCAAATTTACAACCTCTGTTGTTCTCATTTAGTGAGAGCACCAGAGCTCATATAGCAGCCAAAATTGAAAGTCTGGAGAAGGAGATCGCTATACTTTCGCGTTATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

404

Amino Acids

45.47

Weight (kDa)

5.33

Isoelectric Point (pI)

57.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 316 - 363 2.3e-17 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 862
AccBSI CCGCTC 1 cut(s) 941
AccII CGCG 4 cut(s) 525, 558, 658, 1204
AciI CCGC 6 cut(s) 395, 525, 658, 941, 1020, 1045
AclWI GGATC 5 cut(s) 303, 456, 698, 711, 969
AcsI RAATTY 6 cut(s) 226, 463, 503, 510, 829, 1108
AcuI CTGAAG 2 cut(s) 86, 801
AcyI GRCGYC 2 cut(s) 528, 866
AfaI GTAC 3 cut(s) 32, 135, 222
AfiI CCNNNNNNNGG 3 cut(s) 222, 737, 879
AgsI TTSAA 6 cut(s) 509, 548, 992, 1095, 1107, 1172
AjnI CCWGG 1 cut(s) 1069
AjuI GAANNNNNNNTTGG 2 cut(s) 938, 970
AluBI AGCT 5 cut(s) 326, 609, 1025, 1151, 1212
AluI AGCT 5 cut(s) 326, 609, 1025, 1151, 1212
Alw21I GWGCWC 2 cut(s) 1145, 1153
AlwI GGATC 5 cut(s) 303, 456, 698, 711, 969
AlwNI CAGNNNCTG 1 cut(s) 164
Ama87I CYCGRG 1 cut(s) 794
AoxI GGCC 2 cut(s) 678, 1067
ApeKI GCWGC 3 cut(s) 712, 1022, 1160
ApoI RAATTY 6 cut(s) 226, 463, 503, 510, 829, 1108
Asp700I GAANNNNTTC 2 cut(s) 765, 789
AspA2I CCTAGG 1 cut(s) 908
AspLEI GCGC 2 cut(s) 560, 849
AspS9I GGNCC 1 cut(s) 893
AsuC2I CCSGG 1 cut(s) 874
AsuHPI GGTGA 1 cut(s) 976
AsuII TTCGAA 1 cut(s) 417
AvaI CYCGRG 1 cut(s) 794
AvaII GGWCC 1 cut(s) 893
AvrII CCTAGG 1 cut(s) 908
BamHI GGATCC 1 cut(s) 703
BanII GRGCYC 1 cut(s) 1153
BbsI GAAGAC 1 cut(s) 559
Bbv12I GWGCWC 2 cut(s) 1145, 1153
BbvI GCAGC 3 cut(s) 724, 1034, 1172
BccI CCATC 3 cut(s) 148, 987, 1059
BceAI ACGGC 1 cut(s) 13
BciT130I CCWGG 1 cut(s) 1071
BcnI CCSGG 1 cut(s) 874
BfaI CTAG 3 cut(s) 243, 392, 909
BfoI RGCGCY 1 cut(s) 850
BfuAI ACCTGC 1 cut(s) 862
BglII AGATCT 1 cut(s) 346
BisI GCNGC 6 cut(s) 526, 713, 942, 1023, 1045, 1161
BlnI CCTAGG 1 cut(s) 908
BlpI GCTNAGC 1 cut(s) 900
BlsI GCNGC 6 cut(s) 527, 714, 943, 1024, 1046, 1162
Bme1390I CCNGG 2 cut(s) 874, 1071
Bme18I GGWCC 1 cut(s) 893
BmeT110I CYCGRG 1 cut(s) 794
BmgT120I GGNCC 1 cut(s) 893
BmiI GGNNCC 2 cut(s) 705, 894
BmrFI CCNGG 2 cut(s) 874, 1071
BmrI ACTGGG 1 cut(s) 212
BmuI ACTGGG 1 cut(s) 212
BpiI GAAGAC 1 cut(s) 559
BpmI CTGGAG 1 cut(s) 1199
Bpu1102I GCTNAGC 1 cut(s) 900
Bpu14I TTCGAA 1 cut(s) 417
BpuMI CCSGG 1 cut(s) 874
BsaHI GRCGYC 2 cut(s) 528, 866
BsaJI CCNNGG 3 cut(s) 14, 795, 908
BsaWI WCCGGW 2 cut(s) 445, 578
Bsc4I CCNNNNNNNGG 3 cut(s) 222, 737, 879
Bse1I ACTGG 3 cut(s) 218, 252, 588
BseBI CCWGG 1 cut(s) 1071
BseDI CCNNGG 3 cut(s) 14, 795, 908
BseGI GGATG 4 cut(s) 25, 140, 180, 751
BseLI CCNNNNNNNGG 3 cut(s) 222, 737, 879
BseMII CTCAG 3 cut(s) 59, 318, 993
BseNI ACTGG 3 cut(s) 218, 252, 588
BseRI GAGGAG 4 cut(s) 276, 279, 282, 683
BseXI GCAGC 3 cut(s) 724, 1034, 1172
Bsh1236I CGCG 4 cut(s) 525, 558, 658, 1204
BshFI GGCC 2 cut(s) 680, 1069
BsiHKAI GWGCWC 2 cut(s) 1145, 1153
BsiHKCI CYCGRG 1 cut(s) 794
BsiSI CCGG 3 cut(s) 446, 579, 873
BslFI GGGAC 1 cut(s) 41
BslI CCNNNNNNNGG 3 cut(s) 222, 737, 879
BsmFI GGGAC 1 cut(s) 41
BsnI GGCC 2 cut(s) 680, 1069
BsoBI CYCGRG 1 cut(s) 794
Bsp119I TTCGAA 1 cut(s) 417
Bsp1286I GDGCHC 2 cut(s) 1145, 1153
Bsp143I GATC 9 cut(s) 91, 295, 346, 448, 703, 820, 961, 1080, 1188
Bsp1720I GCTNAGC 1 cut(s) 900
Bsp19I CCATGG 1 cut(s) 14
BspACI CCGC 6 cut(s) 395, 525, 658, 941, 1020, 1045
BspANI GGCC 2 cut(s) 680, 1069
BspCNI CTCAG 3 cut(s) 60, 317, 994
BspFNI CGCG 4 cut(s) 525, 558, 658, 1204
BspLI GGNNCC 2 cut(s) 705, 894
BspMI ACCTGC 1 cut(s) 862
BspPI GGATC 5 cut(s) 303, 456, 698, 711, 969
BspT104I TTCGAA 1 cut(s) 417
BsrBI CCGCTC 1 cut(s) 941
BsrI ACTGG 3 cut(s) 218, 252, 588
BssECI CCNNGG 3 cut(s) 14, 795, 908
BssMI GATC 9 cut(s) 91, 295, 346, 448, 703, 820, 961, 1080, 1188
BssNI GRCGYC 2 cut(s) 528, 866
BssT1I CCWWGG 2 cut(s) 14, 908
Bst2UI CCWGG 1 cut(s) 1071
Bst4CI ACNGT 1 cut(s) 291
Bst6I CTCTTC 1 cut(s) 987
BstACI GRCGYC 2 cut(s) 528, 866
BstBI TTCGAA 1 cut(s) 417
BstDEI CTNAG 5 cut(s) 68, 95, 304, 900, 1002
BstDSI CCRYGG 1 cut(s) 14
BstF5I GGATG 4 cut(s) 25, 140, 180, 751
BstFNI CGCG 4 cut(s) 525, 558, 658, 1204
BstH2I RGCGCY 1 cut(s) 850
BstHHI GCGC 2 cut(s) 560, 849
BstKTI GATC 9 cut(s) 94, 298, 349, 451, 706, 823, 964, 1083, 1191
BstMBI GATC 9 cut(s) 91, 295, 346, 448, 703, 820, 961, 1080, 1188
BstMWI GCNNNNNNNGC 4 cut(s) 627, 863, 910, 1157
BstNI CCWGG 1 cut(s) 1071
BstSCI CCNGG 2 cut(s) 872, 1069
BstUI CGCG 4 cut(s) 525, 558, 658, 1204
BstV1I GCAGC 3 cut(s) 724, 1034, 1172
BstV2I GAAGAC 1 cut(s) 559
BstX2I RGATCY 4 cut(s) 346, 448, 703, 961
BstYI RGATCY 4 cut(s) 346, 448, 703, 961
BsuRI GGCC 2 cut(s) 680, 1069
BtgI CCRYGG 1 cut(s) 14
BtsCI GGATG 4 cut(s) 25, 140, 180, 751
BtsIMutI CAGTG 2 cut(s) 287, 595
BveI ACCTGC 1 cut(s) 862
CaiI CAGNNNCTG 1 cut(s) 164
CfoI GCGC 2 cut(s) 560, 849
Cfr13I GGNCC 1 cut(s) 893
CseI GACGC 3 cut(s) 517, 855, 893
Csp6I GTAC 3 cut(s) 31, 134, 221
CviAII CATG 5 cut(s) 15, 35, 155, 515, 539
CviQI GTAC 3 cut(s) 31, 134, 221
DdeI CTNAG 5 cut(s) 68, 95, 304, 900, 1002
DpnI GATC 9 cut(s) 93, 297, 348, 450, 705, 822, 963, 1082, 1190
DpnII GATC 9 cut(s) 91, 295, 346, 448, 703, 820, 961, 1080, 1188
Eam1104I CTCTTC 1 cut(s) 987
EarI CTCTTC 1 cut(s) 987
Ecl136II GAGCTC 1 cut(s) 1151
Eco130I CCWWGG 2 cut(s) 14, 908
Eco24I GRGCYC 1 cut(s) 1153
Eco47I GGWCC 1 cut(s) 893
Eco53kI GAGCTC 1 cut(s) 1151
Eco57I CTGAAG 2 cut(s) 86, 801
Eco88I CYCGRG 1 cut(s) 794
EcoICRI GAGCTC 1 cut(s) 1151
EcoO109I RGGNCCY 1 cut(s) 893
EcoRII CCWGG 1 cut(s) 1069
EcoT14I CCWWGG 2 cut(s) 14, 908
EcoT38I GRGCYC 1 cut(s) 1153
ErhI CCWWGG 2 cut(s) 14, 908
FaeI CATG 5 cut(s) 18, 38, 158, 518, 542
FaqI GGGAC 1 cut(s) 41
FatI CATG 5 cut(s) 14, 34, 154, 514, 538
Fnu4HI GCNGC 6 cut(s) 526, 713, 942, 1023, 1045, 1161
FokI GGATG 4 cut(s) 32, 127, 167, 758
FriOI GRGCYC 1 cut(s) 1153
Fsp4HI GCNGC 6 cut(s) 526, 713, 942, 1023, 1045, 1161
FspBI CTAG 3 cut(s) 243, 392, 909
GlaI GCGC 2 cut(s) 559, 848
GluI GCNGC 6 cut(s) 526, 713, 942, 1023, 1045, 1161
GsuI CTGGAG 1 cut(s) 1199
HaeII RGCGCY 1 cut(s) 850
HaeIII GGCC 2 cut(s) 680, 1069
HapII CCGG 3 cut(s) 446, 579, 873
HgaI GACGC 3 cut(s) 517, 855, 893
HhaI GCGC 2 cut(s) 560, 849
Hin1I GRCGYC 2 cut(s) 528, 866
Hin1II CATG 5 cut(s) 18, 38, 158, 518, 542
Hin6I GCGC 2 cut(s) 558, 847
HinP1I GCGC 2 cut(s) 558, 847
HinfI GANTC 2 cut(s) 83, 274
HpaII CCGG 3 cut(s) 446, 579, 873
HphI GGTGA 1 cut(s) 976
Hpy166II GTNNAC 2 cut(s) 593, 973
Hpy188III TCNNGA 6 cut(s) 50, 548, 779, 794, 923, 1178
Hpy8I GTNNAC 2 cut(s) 593, 973
Hpy99I CGWCG 3 cut(s) 460, 558, 871
HpyAV CCTTC 5 cut(s) 110, 677, 883, 929, 1177
HpyCH4III ACNGT 1 cut(s) 291
HpyCH4IV ACGT 2 cut(s) 126, 553
HpyCH4V TGCA 3 cut(s) 158, 542, 712
HpyF10VI GCNNNNNNNGC 4 cut(s) 627, 863, 910, 1157
HpyF3I CTNAG 5 cut(s) 68, 95, 304, 900, 1002
HpySE526I ACGT 2 cut(s) 126, 553
Hsp92I GRCGYC 2 cut(s) 528, 866
Hsp92II CATG 5 cut(s) 18, 38, 158, 518, 542
HspAI GCGC 2 cut(s) 558, 847
Kzo9I GATC 9 cut(s) 91, 295, 346, 448, 703, 820, 961, 1080, 1188
LmnI GCTCC 3 cut(s) 368, 670, 844
Lsp1109I GCAGC 3 cut(s) 724, 1034, 1172
MaeI CTAG 3 cut(s) 243, 392, 909
MaeII ACGT 2 cut(s) 126, 553
MalI GATC 9 cut(s) 93, 297, 348, 450, 705, 822, 963, 1082, 1190
MbiI CCGCTC 1 cut(s) 941
MboI GATC 9 cut(s) 91, 295, 346, 448, 703, 820, 961, 1080, 1188
MboII GAAGA 8 cut(s) 341, 406, 559, 761, 773, 794, 958, 1004
MfeI CAATTG 1 cut(s) 698
MflI RGATCY 4 cut(s) 346, 448, 703, 961
MhlI GDGCHC 2 cut(s) 1145, 1153
MlyI GAGTC 1 cut(s) 283
MmeI TCCRAC 4 cut(s) 102, 273, 302, 718
MroXI GAANNNNTTC 2 cut(s) 765, 789
MseI TTAA 1 cut(s) 210
MslI CAYNNNNRTG 1 cut(s) 153
MspA1I CMGCKG 1 cut(s) 1025
MspI CCGG 3 cut(s) 446, 579, 873
MspR9I CCNGG 2 cut(s) 874, 1071
MunI CAATTG 1 cut(s) 698
MvaI CCWGG 1 cut(s) 1071
MvnI CGCG 4 cut(s) 525, 558, 658, 1204
MwoI GCNNNNNNNGC 4 cut(s) 627, 863, 910, 1157
NciI CCSGG 1 cut(s) 874
NcoI CCATGG 1 cut(s) 14
NdeII GATC 9 cut(s) 91, 295, 346, 448, 703, 820, 961, 1080, 1188
NlaIII CATG 5 cut(s) 18, 38, 158, 518, 542
NlaIV GGNNCC 2 cut(s) 705, 894
NmeAIII GCCGAG 1 cut(s) 92
NspV TTCGAA 1 cut(s) 417
PdmI GAANNNNTTC 2 cut(s) 765, 789
PfeI GAWTC 1 cut(s) 83
PfoI TCCNGGA 1 cut(s) 872
PkrI GCNGC 6 cut(s) 527, 714, 943, 1024, 1046, 1162
PleI GAGTC 1 cut(s) 282
PpsI GAGTC 1 cut(s) 282
PpuMI RGGWCCY 1 cut(s) 893
Psp124BI GAGCTC 1 cut(s) 1153
Psp5II RGGWCCY 1 cut(s) 893
Psp6I CCWGG 1 cut(s) 1069
PspGI CCWGG 1 cut(s) 1069
PspN4I GGNNCC 2 cut(s) 705, 894
PspPI GGNCC 1 cut(s) 893
PspPPI RGGWCCY 1 cut(s) 893
PstNI CAGNNNCTG 1 cut(s) 164
PsuI RGATCY 4 cut(s) 346, 448, 703, 961
PvuII CAGCTG 1 cut(s) 1025
RsaI GTAC 3 cut(s) 32, 135, 222
RsaNI GTAC 3 cut(s) 31, 134, 221
RseI CAYNNNNRTG 1 cut(s) 153
SacI GAGCTC 1 cut(s) 1153
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 6 cut(s) 526, 713, 942, 1023, 1045, 1161
Sau3AI GATC 9 cut(s) 91, 295, 346, 448, 703, 820, 961, 1080, 1188
Sau96I GGNCC 1 cut(s) 893
SchI GAGTC 1 cut(s) 283
ScrFI CCNGG 2 cut(s) 874, 1071
SduI GDGCHC 2 cut(s) 1145, 1153
SfuI TTCGAA 1 cut(s) 417
SinI GGWCC 1 cut(s) 893
SmiMI CAYNNNNRTG 1 cut(s) 153
SsiI CCGC 6 cut(s) 395, 525, 658, 941, 1020, 1045
SspI AATATT 1 cut(s) 433
SspMI CTAG 3 cut(s) 243, 392, 909
SstI GAGCTC 1 cut(s) 1153
StyD4I CCNGG 2 cut(s) 872, 1069
StyI CCWWGG 2 cut(s) 14, 908
TaaI ACNGT 1 cut(s) 291
TaiI ACGT 2 cut(s) 129, 556
TaqI TCGA 3 cut(s) 417, 612, 819
TauI GCSGC 3 cut(s) 528, 944, 1047
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 2 cut(s) 294, 595
TseI GCWGC 3 cut(s) 712, 1022, 1160
TspDTI ATGAA 4 cut(s) 503, 762, 852, 965
TspGWI ACGGA 3 cut(s) 238, 901, 956
TspRI CASTG 2 cut(s) 294, 595
VpaK11BI GGWCC 1 cut(s) 893
XapI RAATTY 6 cut(s) 226, 463, 503, 510, 829, 1108
XmaJI CCTAGG 1 cut(s) 908
XmnI GAANNNNTTC 2 cut(s) 765, 789
XspI CTAG 3 cut(s) 243, 392, 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.