MD15G1052600.v1.1

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006657
Physical Location & Seq
Forward (+)
3608515 .. 3610155
1641 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1052600.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGGCAGATCCCAGGGCTGTTGTACCGTACCATGACCCGTACGATAACGGCCCGTGTTCCGATATTGTGAACTTTCTGTACGATTCTGAACACCCGATCCTCGACGAGAGCTTACCGTTACCGCCATTGGACGATGAGGGACTTGCTTGCCCTACTAAACCAATTCCTCTTTTTGATGAAGGAATCCAAGATTTGAGCGACCCTTCGTTGGATCAAGCGACATGGGATTTTCTCTATCAGTGTAGTAATGGCGGAGGAAACTTTGATTCGCAAGCCGGTCCCTCGCAGTTTATCGGAGAATCGAGCGGCTCTAGTCCTCCCAGAGGGGACAACAATGAGCAGAATCAGGGTTTTGGTGATCCGAGGCCGCTTTCGGTTTGGCCTGCGACACCAGTTCCATTTAATTGCAGTTGTTGCCAACTGCTTAGGGAAATTATACACTTAAATGGCAACCATGTCATGAAACTTGACATTCATGGAAGGTTTGGTACGATCAGTCATGCAATTCTTTACAGTTTCGATGTGAATTCTTCCGACAATCATCAGTATCAAATGTTTGATTTTTGCAAGAAAAGCATAGAGGAGGTGAAGCAATTTCTGATGCAGTACTGCCTAGAGAGAAAGCTAGAGGGCTACATTCTGCAGCAAGATCCAATGGCAGTCTTTTACGAGGCCCTCTGCATTGGACTCGGGTGGGATGACATTCTAAATACCGATGACTTCACCTTCCCACCATCTCCTCCAAATTCAGGTTCACGTGAAGAGTCCGTGAAGTGGAACAACAACAACCCGCTGAAGATCAATCACATGCGGCCGAGGATGAGAACGAAAGGATTTCCCTTGCAGCACAGGGAGAGAACAGCAAGCATGACATTGGACGACCTTCGCGATTACTTCCATCTTCCGATCGAAAAAGCTGCTAGGAGATTGAAGGTTTGCCCAACTGTTGTGAAAAGGATATGCCGCCGAAGCGGATTAAATAGATGGCCCTCAAGAAAGATTCGAAGCATCAACAGGCAGATTTCGAGGTTGAGACCAAGTTTAGATTCCGACGATGCAAACACAAGGGTTCATGCTGAAGCTGAAATCTCTAGGCTTGAAAGAAAAATAACCGCACTTGTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

376

Amino Acids

42.64

Weight (kDa)

5.69

Isoelectric Point (pI)

51.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 289 - 337 3.2e-23 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 306
AccII CGCG 1 cut(s) 888
AciI CCGC 9 cut(s) 122, 252, 306, 368, 791, 811, 964, 972, 1113
AclWI GGATC 5 cut(s) 2, 91, 219, 353, 644
AcoI YGGCCR 1 cut(s) 812
AcsI RAATTY 2 cut(s) 526, 745
AcuI CTGAAG 2 cut(s) 815, 1098
AcvI CACGTG 1 cut(s) 758
AfaI GTAC 7 cut(s) 24, 29, 41, 80, 490, 608, 1122
AfiI CCNNNNNNNGG 4 cut(s) 208, 323, 749, 774
AgsI TTSAA 2 cut(s) 931, 1100
AjnI CCWGG 1 cut(s) 11
AjuI GAANNNNNNNTTGG 4 cut(s) 736, 768, 1030, 1062
AloI GAACNNNNNNTCC 2 cut(s) 81, 113
AluBI AGCT 4 cut(s) 111, 625, 917, 1082
AluI AGCT 4 cut(s) 111, 625, 917, 1082
Alw26I GTCTC 1 cut(s) 1027
AlwI GGATC 5 cut(s) 2, 91, 219, 353, 644
Ama87I CYCGRG 1 cut(s) 689
AoxI GGCC 6 cut(s) 49, 365, 380, 672, 812, 986
ApeKI GCWGC 3 cut(s) 643, 844, 917
ApoI RAATTY 2 cut(s) 526, 745
AspS9I GGNCC 4 cut(s) 50, 278, 673, 987
AsuHPI GGTGA 3 cut(s) 368, 598, 715
AsuII TTCGAA 1 cut(s) 1003
AvaI CYCGRG 1 cut(s) 689
AvaII GGWCC 1 cut(s) 278
BarI GAAGNNNNNNTAC 2 cut(s) 472, 504
BbrPI CACGTG 1 cut(s) 758
BbvI GCAGC 3 cut(s) 655, 856, 904
BccI CCATC 3 cut(s) 742, 906, 978
BceAI ACGGC 1 cut(s) 64
BcgI CGANNNNNNTGC 4 cut(s) 670, 704, 899, 933
BciT130I CCWGG 1 cut(s) 13
BcoDI GTCTC 1 cut(s) 1027
BfaI CTAG 5 cut(s) 312, 614, 626, 921, 1092
BfmI CTRYAG 1 cut(s) 641
BisI GCNGC 7 cut(s) 307, 368, 644, 812, 845, 918, 964
BlsI GCNGC 7 cut(s) 308, 369, 645, 813, 846, 919, 965
BmcAI AGTACT 1 cut(s) 608
Bme1390I CCNGG 1 cut(s) 13
Bme18I GGWCC 1 cut(s) 278
BmeT110I CYCGRG 1 cut(s) 689
BmgT120I GGNCC 4 cut(s) 50, 278, 673, 987
BmiI GGNNCC 1 cut(s) 280
BmrFI CCNGG 1 cut(s) 13
BmsI GCATC 3 cut(s) 591, 1017, 1045
Bpu10I CCTNAGC 1 cut(s) 425
Bpu14I TTCGAA 1 cut(s) 1003
BpuEI CTTGAG 1 cut(s) 976
BsaAI YACGTR 1 cut(s) 758
BsaI GGTCTC 1 cut(s) 1027
BsaJI CCNNGG 4 cut(s) 11, 12, 362, 815
Bsc4I CCNNNNNNNGG 4 cut(s) 208, 323, 749, 774
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 1 cut(s) 392
BseBI CCWGG 1 cut(s) 13
BseDI CCNNGG 4 cut(s) 11, 12, 362, 815
BseGI GGATG 2 cut(s) 703, 825
BseLI CCNNNNNNNGG 4 cut(s) 208, 323, 749, 774
BseNI ACTGG 1 cut(s) 392
BseRI GAGGAG 2 cut(s) 596, 729
BseX3I CGGCCG 1 cut(s) 812
BseXI GCAGC 3 cut(s) 655, 856, 904
Bsh1236I CGCG 1 cut(s) 888
Bsh1285I CGRYCG 2 cut(s) 815, 909
BshFI GGCC 6 cut(s) 51, 367, 382, 674, 814, 988
BsiEI CGRYCG 2 cut(s) 815, 909
BsiHKCI CYCGRG 1 cut(s) 689
BsiSI CCGG 1 cut(s) 276
BsiWI CGTACG 1 cut(s) 39
BslFI GGGAC 3 cut(s) 153, 264, 341
BslI CCNNNNNNNGG 4 cut(s) 208, 323, 749, 774
BsmAI GTCTC 1 cut(s) 1027
BsmFI GGGAC 3 cut(s) 153, 264, 341
BsnI GGCC 6 cut(s) 51, 367, 382, 674, 814, 988
Bso31I GGTCTC 1 cut(s) 1027
BsoBI CYCGRG 1 cut(s) 689
Bsp119I TTCGAA 1 cut(s) 1003
Bsp143I GATC 8 cut(s) 7, 96, 211, 358, 492, 649, 798, 906
Bsp68I TCGCGA 1 cut(s) 888
BspACI CCGC 9 cut(s) 122, 252, 306, 368, 791, 811, 964, 972, 1113
BspANI GGCC 6 cut(s) 51, 367, 382, 674, 814, 988
BspFNI CGCG 1 cut(s) 888
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 1 cut(s) 280
BspMAI CTGCAG 1 cut(s) 645
BspPI GGATC 5 cut(s) 2, 91, 219, 353, 644
BspT104I TTCGAA 1 cut(s) 1003
BspTNI GGTCTC 1 cut(s) 1027
BsrBI CCGCTC 1 cut(s) 306
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 1 cut(s) 392
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 4 cut(s) 11, 12, 362, 815
BssMI GATC 8 cut(s) 7, 96, 211, 358, 492, 649, 798, 906
Bst2UI CCWGG 1 cut(s) 13
Bst4CI ACNGT 4 cut(s) 27, 117, 515, 946
Bst6I CTCTTC 1 cut(s) 756
BstAPI GCANNNNNTGC 1 cut(s) 414
BstBAI YACGTR 1 cut(s) 758
BstBI TTCGAA 1 cut(s) 1003
BstC8I GCNNGC 4 cut(s) 148, 273, 384, 865
BstDEI CTNAG 1 cut(s) 425
BstENI CCTNNNNNAGG 1 cut(s) 321
BstF5I GGATG 2 cut(s) 703, 825
BstFNI CGCG 1 cut(s) 888
BstKTI GATC 8 cut(s) 10, 99, 214, 361, 495, 652, 801, 909
BstMAI GTCTC 1 cut(s) 1027
BstMBI GATC 8 cut(s) 7, 96, 211, 358, 492, 649, 798, 906
BstMCI CGRYCG 2 cut(s) 815, 909
BstMWI GCNNNNNNNGC 3 cut(s) 414, 573, 969
BstNI CCWGG 1 cut(s) 13
BstNSI RCATGY 1 cut(s) 811
BstSCI CCNGG 1 cut(s) 11
BstSFI CTRYAG 1 cut(s) 641
BstUI CGCG 1 cut(s) 888
BstV1I GCAGC 3 cut(s) 655, 856, 904
BstX2I RGATCY 2 cut(s) 7, 649
BstYI RGATCY 2 cut(s) 7, 649
BstZI CGGCCG 1 cut(s) 812
BsuRI GGCC 6 cut(s) 51, 367, 382, 674, 814, 988
BtsCI GGATG 2 cut(s) 703, 825
BtsIMutI CAGTG 1 cut(s) 245
BtuMI TCGCGA 1 cut(s) 888
Cac8I GCNNGC 4 cut(s) 148, 273, 384, 865
CciI TCATGA 1 cut(s) 459
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 4 cut(s) 50, 278, 673, 987
Csp6I GTAC 7 cut(s) 23, 28, 40, 79, 489, 607, 1121
CviAII CATG 9 cut(s) 32, 222, 455, 460, 476, 500, 808, 868, 1073
CviQI GTAC 7 cut(s) 23, 28, 40, 79, 489, 607, 1121
DdeI CTNAG 1 cut(s) 425
DpnI GATC 8 cut(s) 9, 98, 213, 360, 494, 651, 800, 908
DpnII GATC 8 cut(s) 7, 96, 211, 358, 492, 649, 798, 906
EaeI YGGCCR 1 cut(s) 812
EagI CGGCCG 1 cut(s) 812
Eam1104I CTCTTC 1 cut(s) 756
EarI CTCTTC 1 cut(s) 756
EciI GGCGGA 1 cut(s) 267
EclXI CGGCCG 1 cut(s) 812
Eco31I GGTCTC 1 cut(s) 1027
Eco47I GGWCC 1 cut(s) 278
Eco52I CGGCCG 1 cut(s) 812
Eco57I CTGAAG 2 cut(s) 815, 1098
Eco72I CACGTG 1 cut(s) 758
Eco88I CYCGRG 1 cut(s) 689
EcoNI CCTNNNNNAGG 1 cut(s) 321
EcoO109I RGGNCCY 1 cut(s) 673
EcoRI GAATTC 1 cut(s) 526
EcoRII CCWGG 1 cut(s) 11
FaeI CATG 9 cut(s) 35, 225, 458, 463, 479, 503, 811, 871, 1076
FaqI GGGAC 3 cut(s) 153, 264, 341
FatI CATG 9 cut(s) 31, 221, 454, 459, 475, 499, 807, 867, 1072
FauI CCCGC 1 cut(s) 798
Fnu4HI GCNGC 7 cut(s) 307, 368, 644, 812, 845, 918, 964
FokI GGATG 2 cut(s) 710, 832
Fsp4HI GCNGC 7 cut(s) 307, 368, 644, 812, 845, 918, 964
FspBI CTAG 5 cut(s) 312, 614, 626, 921, 1092
GluI GCNGC 7 cut(s) 307, 368, 644, 812, 845, 918, 964
HaeIII GGCC 6 cut(s) 51, 367, 382, 674, 814, 988
HapII CCGG 1 cut(s) 276
Hin1II CATG 9 cut(s) 35, 225, 458, 463, 479, 503, 811, 871, 1076
HinfI GANTC 9 cut(s) 83, 183, 266, 299, 343, 687, 764, 1000, 1046
HpaII CCGG 1 cut(s) 276
HphI GGTGA 3 cut(s) 368, 598, 715
Hpy166II GTNNAC 2 cut(s) 70, 755
Hpy188I TCNGA 8 cut(s) 61, 88, 296, 363, 535, 600, 906, 1051
Hpy188III TCNNGA 3 cut(s) 460, 887, 993
Hpy8I GTNNAC 2 cut(s) 70, 755
Hpy99I CGWCG 2 cut(s) 107, 1055
HpyAV CCTTC 6 cut(s) 173, 213, 474, 736, 893, 925
HpyCH4III ACNGT 4 cut(s) 27, 117, 515, 946
HpyCH4IV ACGT 1 cut(s) 757
HpyCH4V TGCA 8 cut(s) 408, 503, 567, 604, 643, 681, 844, 1058
HpyF10VI GCNNNNNNNGC 3 cut(s) 414, 573, 969
HpyF3I CTNAG 1 cut(s) 425
HpySE526I ACGT 1 cut(s) 757
Hsp92II CATG 9 cut(s) 35, 225, 458, 463, 479, 503, 811, 871, 1076
Kzo9I GATC 8 cut(s) 7, 96, 211, 358, 492, 649, 798, 906
LpnPI CCDG 9 cut(s) 25, 289, 332, 334, 396, 405, 735, 835, 1000
Lsp1109I GCAGC 3 cut(s) 655, 856, 904
LweI GCATC 3 cut(s) 591, 1017, 1045
MaeI CTAG 5 cut(s) 312, 614, 626, 921, 1092
MaeII ACGT 1 cut(s) 757
MaeIII GTNAC 1 cut(s) 117
MalI GATC 8 cut(s) 9, 98, 213, 360, 494, 651, 800, 908
MbiI CCGCTC 1 cut(s) 306
MboI GATC 8 cut(s) 7, 96, 211, 358, 492, 649, 798, 906
MboII GAAGA 4 cut(s) 522, 773, 808, 893
MflI RGATCY 2 cut(s) 7, 649
MluCI AATT 7 cut(s) 162, 403, 432, 504, 526, 593, 745
MlyI GAGTC 2 cut(s) 681, 773
MmeI TCCRAC 3 cut(s) 189, 558, 1074
MseI TTAA 3 cut(s) 402, 443, 977
MslI CAYNNNNRTG 1 cut(s) 444
MspA1I CMGCKG 1 cut(s) 793
MspI CCGG 1 cut(s) 276
MspR9I CCNGG 1 cut(s) 13
MvaI CCWGG 1 cut(s) 13
MvnI CGCG 1 cut(s) 888
MwoI GCNNNNNNNGC 3 cut(s) 414, 573, 969
NdeII GATC 8 cut(s) 7, 96, 211, 358, 492, 649, 798, 906
NlaIII CATG 9 cut(s) 35, 225, 458, 463, 479, 503, 811, 871, 1076
NlaIV GGNNCC 1 cut(s) 280
NmeAIII GCCGAG 1 cut(s) 840
NruI TCGCGA 1 cut(s) 888
NspI RCATGY 1 cut(s) 811
NspV TTCGAA 1 cut(s) 1003
PagI TCATGA 1 cut(s) 459
PasI CCCWGGG 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 885
PfeI GAWTC 7 cut(s) 83, 183, 266, 299, 343, 1000, 1046
Pfl23II CGTACG 1 cut(s) 39
PkrI GCNGC 7 cut(s) 308, 369, 645, 813, 846, 919, 965
Ple19I CGATCG 1 cut(s) 909
PleI GAGTC 2 cut(s) 681, 772
PmaCI CACGTG 1 cut(s) 758
PmlI CACGTG 1 cut(s) 758
PpsI GAGTC 2 cut(s) 681, 772
Ppu21I YACGTR 1 cut(s) 758
Psp6I CCWGG 1 cut(s) 11
PspCI CACGTG 1 cut(s) 758
PspGI CCWGG 1 cut(s) 11
PspLI CGTACG 1 cut(s) 39
PspN4I GGNNCC 1 cut(s) 280
PspPI GGNCC 4 cut(s) 50, 278, 673, 987
PsrI GAACNNNNNNTAC 2 cut(s) 62, 94
PstI CTGCAG 1 cut(s) 645
PsuI RGATCY 2 cut(s) 7, 649
PvuI CGATCG 1 cut(s) 909
RruI TCGCGA 1 cut(s) 888
RsaI GTAC 7 cut(s) 24, 29, 41, 80, 490, 608, 1122
RsaNI GTAC 7 cut(s) 23, 28, 40, 79, 489, 607, 1121
RseI CAYNNNNRTG 1 cut(s) 444
SaqAI TTAA 3 cut(s) 402, 443, 977
SatI GCNGC 7 cut(s) 307, 368, 644, 812, 845, 918, 964
Sau3AI GATC 8 cut(s) 7, 96, 211, 358, 492, 649, 798, 906
Sau96I GGNCC 4 cut(s) 50, 278, 673, 987
ScaI AGTACT 1 cut(s) 608
SchI GAGTC 2 cut(s) 681, 773
ScrFI CCNGG 1 cut(s) 13
SfaNI GCATC 3 cut(s) 591, 1017, 1045
SfcI CTRYAG 1 cut(s) 641
SfuI TTCGAA 1 cut(s) 1003
SinI GGWCC 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 444
SmlI CTYRAG 1 cut(s) 991
SmoI CTYRAG 1 cut(s) 991
Sse9I AATT 7 cut(s) 162, 403, 432, 504, 526, 593, 745
SsiI CCGC 9 cut(s) 122, 252, 306, 368, 791, 811, 964, 972, 1113
SspMI CTAG 5 cut(s) 312, 614, 626, 921, 1092
StyD4I CCNGG 1 cut(s) 11
TaaI ACNGT 4 cut(s) 27, 117, 515, 946
TaiI ACGT 1 cut(s) 760
TaqI TCGA 6 cut(s) 102, 302, 519, 909, 1003, 1025
TasI AATT 7 cut(s) 162, 403, 432, 504, 526, 593, 745
TatI WGTACW 1 cut(s) 606
TauI GCSGC 4 cut(s) 309, 370, 814, 966
TfiI GAWTC 7 cut(s) 83, 183, 266, 299, 343, 1000, 1046
Tru1I TTAA 3 cut(s) 402, 443, 977
Tru9I TTAA 3 cut(s) 402, 443, 977
TscAI CASTG 1 cut(s) 245
TseI GCWGC 3 cut(s) 643, 844, 917
TspDTI ATGAA 4 cut(s) 192, 464, 476, 1061
TspGWI ACGGA 1 cut(s) 757
TspRI CASTG 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 278
XagI CCTNNNNNAGG 1 cut(s) 321
XapI RAATTY 2 cut(s) 526, 745
XceI RCATGY 1 cut(s) 811
XspI CTAG 5 cut(s) 312, 614, 626, 921, 1092
ZrmI AGTACT 1 cut(s) 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.