Rw1G015400

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006657
Physical Location & Seq
Forward (+)
34370769 .. 34371448
680 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G015400.1

Sequence Viewer

Length: 474 bp
ATGGATGAAGATGAGTGGGAAGATGTTCTAACCTATCCTGAAGAATACATTCCCGAGGACTCGGATGTTGACGACTTCGATCTACCAAATTTAGATGAAATGGAGCGCCAACCTGCTGATAAGGCGTCGTCCGGGAATTGGAAACGGAAGGGTCCTGCTAAGCGTCCTAGGCTTTCCCTTCCAGAACAGAGGGAGAGAGCGGCGAAGATGAAATTGGAGGATCTCCGTGTTTACTTCCATCTCACCTTTGAAGAGGCTTGTGAGGAATTGCGATTATCCGCAGCTGTTGTCAAAAGGCTCTGCCGCAAGTTTGGAATAGGCAGATGGCCTGGTAGAAAGATCAAAGCCATTTCAAAGATGATTTCAAATTTACAACCTCTGTTGTTCTCATATAGTGAGAGCACCAGAGTTCATATAGCAGCCAAAATTGAAAGTCTGGAGAAGGAGATCGCTATACTTTCGCGGTATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

157

Amino Acids

18.3

Weight (kDa)

5.59

Isoelectric Point (pI)

64.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 69 - 116 2.1e-18 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 121
AccBSI CCGCTC 1 cut(s) 200
AccII CGCG 1 cut(s) 463
AciI CCGC 4 cut(s) 200, 279, 304, 463
AclWI GGATC 1 cut(s) 228
AcsI RAATTY 2 cut(s) 88, 367
AcuI CTGAAG 1 cut(s) 60
AcyI GRCGYC 1 cut(s) 125
AfiI CCNNNNNNNGG 1 cut(s) 138
AgsI TTSAA 4 cut(s) 251, 354, 366, 431
AjnI CCWGG 1 cut(s) 328
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 2 cut(s) 284, 471
AluI AGCT 2 cut(s) 284, 471
Alw21I GWGCWC 1 cut(s) 404
AlwI GGATC 1 cut(s) 228
Ama87I CYCGRG 1 cut(s) 53
AoxI GGCC 1 cut(s) 326
ApeKI GCWGC 2 cut(s) 281, 419
ApoI RAATTY 2 cut(s) 88, 367
Asp700I GAANNNNTTC 2 cut(s) 24, 48
AspA2I CCTAGG 1 cut(s) 167
AspLEI GCGC 1 cut(s) 108
AspS9I GGNCC 1 cut(s) 152
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 1 cut(s) 235
AvaI CYCGRG 1 cut(s) 53
AvaII GGWCC 1 cut(s) 152
AvrII CCTAGG 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 404
BbvI GCAGC 2 cut(s) 293, 431
BccI CCATC 2 cut(s) 246, 318
BciT130I CCWGG 1 cut(s) 330
BcnI CCSGG 1 cut(s) 133
BfaI CTAG 1 cut(s) 168
BfoI RGCGCY 1 cut(s) 109
BfuAI ACCTGC 1 cut(s) 121
BisI GCNGC 4 cut(s) 201, 282, 304, 420
BlnI CCTAGG 1 cut(s) 167
BlpI GCTNAGC 1 cut(s) 159
BlsI GCNGC 4 cut(s) 202, 283, 305, 421
Bme1390I CCNGG 2 cut(s) 133, 330
Bme18I GGWCC 1 cut(s) 152
BmeT110I CYCGRG 1 cut(s) 53
BmgT120I GGNCC 1 cut(s) 152
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 2 cut(s) 133, 330
BpmI CTGGAG 1 cut(s) 458
Bpu1102I GCTNAGC 1 cut(s) 159
BpuMI CCSGG 1 cut(s) 133
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 2 cut(s) 54, 167
Bsc4I CCNNNNNNNGG 1 cut(s) 138
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 2 cut(s) 54, 167
BseGI GGATG 2 cut(s) 10, 70
BseLI CCNNNNNNNGG 1 cut(s) 138
BseXI GCAGC 2 cut(s) 293, 431
Bsh1236I CGCG 1 cut(s) 463
BshFI GGCC 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 404
BsiHKCI CYCGRG 1 cut(s) 53
BsiSI CCGG 1 cut(s) 132
BslI CCNNNNNNNGG 1 cut(s) 138
BsnI GGCC 1 cut(s) 328
BsoBI CYCGRG 1 cut(s) 53
Bsp1286I GDGCHC 1 cut(s) 404
Bsp143I GATC 4 cut(s) 79, 220, 339, 447
Bsp1720I GCTNAGC 1 cut(s) 159
BspACI CCGC 4 cut(s) 200, 279, 304, 463
BspANI GGCC 1 cut(s) 328
BspFNI CGCG 1 cut(s) 463
BspLI GGNNCC 1 cut(s) 153
BspMI ACCTGC 1 cut(s) 121
BspPI GGATC 1 cut(s) 228
BsrBI CCGCTC 1 cut(s) 200
BssECI CCNNGG 2 cut(s) 54, 167
BssMI GATC 4 cut(s) 79, 220, 339, 447
BssNI GRCGYC 1 cut(s) 125
BssT1I CCWWGG 1 cut(s) 167
Bst2UI CCWGG 1 cut(s) 330
Bst6I CTCTTC 1 cut(s) 246
BstACI GRCGYC 1 cut(s) 125
BstDEI CTNAG 1 cut(s) 159
BstF5I GGATG 2 cut(s) 10, 70
BstFNI CGCG 1 cut(s) 463
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 1 cut(s) 108
BstKTI GATC 4 cut(s) 82, 223, 342, 450
BstMBI GATC 4 cut(s) 79, 220, 339, 447
BstMWI GCNNNNNNNGC 2 cut(s) 122, 169
BstNI CCWGG 1 cut(s) 330
BstSCI CCNGG 2 cut(s) 131, 328
BstUI CGCG 1 cut(s) 463
BstV1I GCAGC 2 cut(s) 293, 431
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
BsuRI GGCC 1 cut(s) 328
BtsCI GGATG 2 cut(s) 10, 70
BveI ACCTGC 1 cut(s) 121
CfoI GCGC 1 cut(s) 108
Cfr13I GGNCC 1 cut(s) 152
CseI GACGC 2 cut(s) 114, 152
CviJI RGCY 8 cut(s) 172, 257, 284, 298, 328, 347, 422, 471
CviKI_1 RGCY 8 cut(s) 172, 257, 284, 298, 328, 347, 422, 471
DdeI CTNAG 1 cut(s) 159
DpnI GATC 4 cut(s) 81, 222, 341, 449
DpnII GATC 4 cut(s) 79, 220, 339, 447
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 167
Eco47I GGWCC 1 cut(s) 152
Eco57I CTGAAG 1 cut(s) 60
Eco88I CYCGRG 1 cut(s) 53
EcoO109I RGGNCCY 1 cut(s) 152
EcoRII CCWGG 1 cut(s) 328
EcoT14I CCWWGG 1 cut(s) 167
ErhI CCWWGG 1 cut(s) 167
FaiI YATR 6 cut(s) 391, 393, 414, 416, 455, 468
Fnu4HI GCNGC 4 cut(s) 201, 282, 304, 420
FokI GGATG 2 cut(s) 17, 77
Fsp4HI GCNGC 4 cut(s) 201, 282, 304, 420
FspBI CTAG 1 cut(s) 168
GlaI GCGC 1 cut(s) 107
GluI GCNGC 4 cut(s) 201, 282, 304, 420
GsuI CTGGAG 1 cut(s) 458
HaeII RGCGCY 1 cut(s) 109
HaeIII GGCC 1 cut(s) 328
HapII CCGG 1 cut(s) 132
HgaI GACGC 2 cut(s) 114, 152
HhaI GCGC 1 cut(s) 108
Hin1I GRCGYC 1 cut(s) 125
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 1 cut(s) 59
HpaII CCGG 1 cut(s) 132
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 2 cut(s) 70, 232
Hpy188I TCNGA 1 cut(s) 64
Hpy188III TCNNGA 4 cut(s) 38, 53, 182, 437
Hpy8I GTNNAC 2 cut(s) 70, 232
Hpy99I CGWCG 1 cut(s) 130
HpyAV CCTTC 3 cut(s) 142, 188, 436
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 169
HpyF3I CTNAG 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 125
HspAI GCGC 1 cut(s) 106
Kzo9I GATC 4 cut(s) 79, 220, 339, 447
LmnI GCTCC 1 cut(s) 103
LpnPI CCDG 9 cut(s) 51, 126, 145, 168, 195, 315, 342, 418, 422
Lsp1109I GCAGC 2 cut(s) 293, 431
MaeI CTAG 1 cut(s) 168
MalI GATC 4 cut(s) 81, 222, 341, 449
MbiI CCGCTC 1 cut(s) 200
MboI GATC 4 cut(s) 79, 220, 339, 447
MboII GAAGA 5 cut(s) 20, 32, 53, 217, 263
MflI RGATCY 1 cut(s) 220
MhlI GDGCHC 1 cut(s) 404
MluCI AATT 6 cut(s) 88, 136, 212, 266, 367, 426
MlyI GAGTC 1 cut(s) 53
MnlI CCTC 6 cut(s) 49, 183, 211, 247, 256, 387
MroXI GAANNNNTTC 2 cut(s) 24, 48
MspA1I CMGCKG 1 cut(s) 284
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 2 cut(s) 133, 330
MvaI CCWGG 1 cut(s) 330
MvnI CGCG 1 cut(s) 463
MwoI GCNNNNNNNGC 2 cut(s) 122, 169
NciI CCSGG 1 cut(s) 133
NdeII GATC 4 cut(s) 79, 220, 339, 447
NlaIV GGNNCC 1 cut(s) 153
PdmI GAANNNNTTC 2 cut(s) 24, 48
PfoI TCCNGGA 1 cut(s) 131
PkrI GCNGC 4 cut(s) 202, 283, 305, 421
PleI GAGTC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 53
PpuMI RGGWCCY 1 cut(s) 152
Psp5II RGGWCCY 1 cut(s) 152
Psp6I CCWGG 1 cut(s) 328
PspGI CCWGG 1 cut(s) 328
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 1 cut(s) 152
PspPPI RGGWCCY 1 cut(s) 152
PsuI RGATCY 1 cut(s) 220
PvuII CAGCTG 1 cut(s) 284
SatI GCNGC 4 cut(s) 201, 282, 304, 420
Sau3AI GATC 4 cut(s) 79, 220, 339, 447
Sau96I GGNCC 1 cut(s) 152
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 2 cut(s) 133, 330
SduI GDGCHC 1 cut(s) 404
SetI ASST 6 cut(s) 35, 115, 248, 286, 379, 473
SinI GGWCC 1 cut(s) 152
Sse9I AATT 6 cut(s) 88, 136, 212, 266, 367, 426
SsiI CCGC 4 cut(s) 200, 279, 304, 463
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 2 cut(s) 131, 328
StyI CCWWGG 1 cut(s) 167
TaqI TCGA 1 cut(s) 78
TasI AATT 6 cut(s) 88, 136, 212, 266, 367, 426
TauI GCSGC 2 cut(s) 203, 306
TseI GCWGC 2 cut(s) 281, 419
TspDTI ATGAA 4 cut(s) 21, 111, 224, 401
TspGWI ACGGA 2 cut(s) 160, 215
VpaK11BI GGWCC 1 cut(s) 152
XapI RAATTY 2 cut(s) 88, 367
XmaJI CCTAGG 1 cut(s) 167
XmnI GAANNNNTTC 2 cut(s) 24, 48
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.