MD08G1069500.v1.1

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006657
Physical Location & Seq
Reverse (-)
5509910 .. 5511605
1696 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1069500.v1.1.491

Sequence Viewer

Length: 1122 bp
ATGGCAGATCCTAGGGCTGTTGTACCGTACCATGACCCGTACGAAAATGGCCTGTGTTCCGATATTATTAACTTTCTGTATGATTCCGAACATCCGATCCTCGATGAGAACGTACCGTTACCGCCTTTGGACGATGAGGGACTCACTTGCCCTACTAATCCAATTTCTCTGTTTGATGAGGGAATCCAAGACTTGGACGACCCTTTGTTGGATCAAGCAACATGGGATTTCCTCAATCAGTGTAGTAATGGCGGAGGGAACGTAGATTCGCAAGCCGGTCCCTCGCATATTATCGGAGAATCGAGTGGTACTAGTCCTCCCAGAGGGGACAACATTGAGCAGAATCAGGATTTTGGTGATCCCGTGCCGCTTTCGGTTTGGCCTGCGATACCGGTTCCATTTAACTGCAGTTGTTGCCAACTGCTTAGGGAAATCATACACTCCAATGGCAATCATGTCATGAAATTTGACATTCATGGAAGGTTCGGTATGATCAGTCATGCAATTCTCCACAGTTTCGATATGAAATCTTCCGACAATCATCAGTATCAAATGTTTGATTTTTGCAAGAAAAGCATAGAGGAGGTGAAGCAATTTCTGATGCAGTACTGCCTAGAGAGAAAGCTAGAGGGATACATTATGCAGCAAGATCCAATGGCAATCTTCTACGAGGCCCTCTGCGTTGGACTCGAGTGGGATGACATTCTAAACACCGATGACTTCACTTTCCCACCATCTCCTCTAAATTCAGGAAGAGCCGGTGAAGTGGAACAACAACAACCCGCTGCTGATCAACTAGAGGCTGCTGAGGATGAGAACGAGAGGATTTCCCTTGCAGCGCAGAGGGAGAGAACAGCAAACATGACGTTGGACGATCTTCGTGGTTACTTCCATCTTCCGATTCAAAAAGCTGCTAGGAGATTGAAGGTTTGCCCAACTGTTGTGAAAAGGATATGCCGCCGAAACGGATTAAACAGATGGCCCTCTAGAAAGATTCGAAGCATCAACAGGCAGATTTCTAGGTTGACACCGAGTTTAGAGTCCGACGACGCAAACACGAGGGTTCATGCTGAAGCTGAAATCTCTAGGCTTGAAAGAGAAATAGTTGAACTTGTCCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

374

Amino Acids

42.19

Weight (kDa)

4.7

Isoelectric Point (pI)

46.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 287 - 335 4.2e-24 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 193
AciI CCGC 5 cut(s) 122, 252, 368, 783, 958
AclWI GGATC 5 cut(s) 2, 91, 219, 353, 644
AcsI RAATTY 2 cut(s) 464, 745
AcuI CTGAAG 1 cut(s) 1092
AfaI GTAC 6 cut(s) 24, 29, 41, 114, 310, 608
AfiI CCNNNNNNNGG 3 cut(s) 193, 208, 323
AgeI ACCGGT 1 cut(s) 391
AgsI TTSAA 4 cut(s) 905, 925, 1094, 1109
AhlI ACTAGT 1 cut(s) 311
AloI GAACNNNNNNTCC 2 cut(s) 81, 113
AluBI AGCT 3 cut(s) 625, 911, 1076
AluI AGCT 3 cut(s) 625, 911, 1076
AlwI GGATC 5 cut(s) 2, 91, 219, 353, 644
Ama87I CYCGRG 1 cut(s) 689
AoxI GGCC 4 cut(s) 49, 380, 672, 980
ApeKI GCWGC 5 cut(s) 643, 785, 803, 836, 911
ApoI RAATTY 2 cut(s) 464, 745
AsiGI ACCGGT 1 cut(s) 391
AspA2I CCTAGG 1 cut(s) 11
AspLEI GCGC 1 cut(s) 841
AspS9I GGNCC 3 cut(s) 278, 673, 981
AsuHPI GGTGA 3 cut(s) 368, 598, 773
AsuII TTCGAA 1 cut(s) 997
AvaI CYCGRG 1 cut(s) 689
AvaII GGWCC 1 cut(s) 278
AvrII CCTAGG 1 cut(s) 11
BauI CACGAG 1 cut(s) 1057
BbvCI CCTCAGC 1 cut(s) 807
BbvI GCAGC 5 cut(s) 655, 772, 790, 848, 898
BccI CCATC 3 cut(s) 742, 900, 972
BciVI GTATCC 1 cut(s) 626
BclI TGATCA 2 cut(s) 492, 790
BcuI ACTAGT 1 cut(s) 311
BfaI CTAG 9 cut(s) 12, 312, 614, 626, 797, 915, 987, 1020, 1086
BfmI CTRYAG 1 cut(s) 406
BfuI GTATCC 1 cut(s) 626
BisI GCNGC 7 cut(s) 368, 644, 786, 804, 837, 912, 958
BlnI CCTAGG 1 cut(s) 11
BlsI GCNGC 7 cut(s) 369, 645, 787, 805, 838, 913, 959
BmcAI AGTACT 1 cut(s) 608
Bme18I GGWCC 1 cut(s) 278
BmeT110I CYCGRG 1 cut(s) 689
BmgT120I GGNCC 3 cut(s) 278, 673, 981
BmiI GGNNCC 2 cut(s) 280, 396
BmsI GCATC 2 cut(s) 591, 1011
Bpu10I CCTNAGC 2 cut(s) 425, 807
Bpu14I TTCGAA 1 cut(s) 997
BsaJI CCNNGG 1 cut(s) 11
BsaWI WCCGGW 1 cut(s) 391
BsaXI ACNNNNNCTCC 4 cut(s) 246, 276, 301, 331
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 208, 323
Bse118I RCCGGY 3 cut(s) 275, 391, 758
BseDI CCNNGG 1 cut(s) 11
BseGI GGATG 3 cut(s) 91, 703, 817
BseLI CCNNNNNNNGG 3 cut(s) 193, 208, 323
BseMII CTCAG 1 cut(s) 798
BseRI GAGGAG 2 cut(s) 596, 729
BseXI GCAGC 5 cut(s) 655, 772, 790, 848, 898
BshFI GGCC 4 cut(s) 51, 382, 674, 982
BshTI ACCGGT 1 cut(s) 391
BsiHKCI CYCGRG 1 cut(s) 689
BsiSI CCGG 3 cut(s) 276, 392, 759
BsiWI CGTACG 1 cut(s) 39
BslFI GGGAC 4 cut(s) 153, 264, 341, 1100
BslI CCNNNNNNNGG 3 cut(s) 193, 208, 323
BsmFI GGGAC 4 cut(s) 153, 264, 341, 1100
BsnI GGCC 4 cut(s) 51, 382, 674, 982
BsoBI CYCGRG 1 cut(s) 689
Bsp119I TTCGAA 1 cut(s) 997
Bsp143I GATC 8 cut(s) 7, 96, 211, 358, 492, 649, 790, 874
BspACI CCGC 5 cut(s) 122, 252, 368, 783, 958
BspANI GGCC 4 cut(s) 51, 382, 674, 982
BspCNI CTCAG 1 cut(s) 799
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 2 cut(s) 280, 396
BspMAI CTGCAG 1 cut(s) 410
BspPI GGATC 5 cut(s) 2, 91, 219, 353, 644
BspQI GCTCTTC 1 cut(s) 748
BspT104I TTCGAA 1 cut(s) 997
BsrFI RCCGGY 3 cut(s) 275, 391, 758
BssAI RCCGGY 3 cut(s) 275, 391, 758
BssECI CCNNGG 1 cut(s) 11
BssMI GATC 8 cut(s) 7, 96, 211, 358, 492, 649, 790, 874
BssSI CACGAG 1 cut(s) 1057
BssT1I CCWWGG 1 cut(s) 11
Bst2BI CACGAG 1 cut(s) 1057
Bst4CI ACNGT 4 cut(s) 27, 117, 515, 940
Bst6I CTCTTC 1 cut(s) 748
BstAPI GCANNNNNTGC 1 cut(s) 414
BstBI TTCGAA 1 cut(s) 997
BstC8I GCNNGC 2 cut(s) 273, 384
BstDEI CTNAG 2 cut(s) 425, 807
BstENI CCTNNNNNAGG 1 cut(s) 321
BstF5I GGATG 3 cut(s) 91, 703, 817
BstHHI GCGC 1 cut(s) 841
BstKTI GATC 8 cut(s) 10, 99, 214, 361, 495, 652, 793, 877
BstMBI GATC 8 cut(s) 7, 96, 211, 358, 492, 649, 790, 874
BstMWI GCNNNNNNNGC 2 cut(s) 414, 573
BstSFI CTRYAG 1 cut(s) 406
BstV1I GCAGC 5 cut(s) 655, 772, 790, 848, 898
BstX2I RGATCY 2 cut(s) 7, 649
BstYI RGATCY 2 cut(s) 7, 649
BsuI GTATCC 1 cut(s) 626
BsuRI GGCC 4 cut(s) 51, 382, 674, 982
BtsCI GGATG 3 cut(s) 91, 703, 817
BtsIMutI CAGTG 1 cut(s) 245
Cac8I GCNNGC 2 cut(s) 273, 384
CciI TCATGA 1 cut(s) 459
CfoI GCGC 1 cut(s) 841
Cfr10I RCCGGY 3 cut(s) 275, 391, 758
Cfr13I GGNCC 3 cut(s) 278, 673, 981
CseI GACGC 1 cut(s) 1058
Csp6I GTAC 6 cut(s) 23, 28, 40, 113, 309, 607
CspAI ACCGGT 1 cut(s) 391
CviAII CATG 8 cut(s) 32, 222, 455, 460, 476, 500, 862, 1067
CviQI GTAC 6 cut(s) 23, 28, 40, 113, 309, 607
DdeI CTNAG 2 cut(s) 425, 807
DpnI GATC 8 cut(s) 9, 98, 213, 360, 494, 651, 792, 876
DpnII GATC 8 cut(s) 7, 96, 211, 358, 492, 649, 790, 874
Eam1104I CTCTTC 1 cut(s) 748
EarI CTCTTC 1 cut(s) 748
EciI GGCGGA 1 cut(s) 267
Eco130I CCWWGG 1 cut(s) 11
Eco47I GGWCC 1 cut(s) 278
Eco57I CTGAAG 1 cut(s) 1092
Eco88I CYCGRG 1 cut(s) 689
EcoNI CCTNNNNNAGG 1 cut(s) 321
EcoO109I RGGNCCY 1 cut(s) 673
EcoT14I CCWWGG 1 cut(s) 11
ErhI CCWWGG 1 cut(s) 11
FaeI CATG 8 cut(s) 35, 225, 458, 463, 479, 503, 865, 1070
FaqI GGGAC 4 cut(s) 153, 264, 341, 1100
FatI CATG 8 cut(s) 31, 221, 454, 459, 475, 499, 861, 1066
FauI CCCGC 1 cut(s) 790
FbaI TGATCA 2 cut(s) 492, 790
Fnu4HI GCNGC 7 cut(s) 368, 644, 786, 804, 837, 912, 958
FokI GGATG 3 cut(s) 78, 710, 824
Fsp4HI GCNGC 7 cut(s) 368, 644, 786, 804, 837, 912, 958
FspBI CTAG 9 cut(s) 12, 312, 614, 626, 797, 915, 987, 1020, 1086
GlaI GCGC 1 cut(s) 840
GluI GCNGC 7 cut(s) 368, 644, 786, 804, 837, 912, 958
HaeIII GGCC 4 cut(s) 51, 382, 674, 982
HapII CCGG 3 cut(s) 276, 392, 759
HgaI GACGC 1 cut(s) 1058
HhaI GCGC 1 cut(s) 841
Hin1II CATG 8 cut(s) 35, 225, 458, 463, 479, 503, 865, 1070
Hin6I GCGC 1 cut(s) 839
HinP1I GCGC 1 cut(s) 839
HincII GTYRAC 1 cut(s) 1026
HindII GTYRAC 1 cut(s) 1026
HpaII CCGG 3 cut(s) 276, 392, 759
HphI GGTGA 3 cut(s) 368, 598, 773
Hpy166II GTNNAC 1 cut(s) 1026
Hpy188I TCNGA 8 cut(s) 61, 88, 96, 296, 535, 600, 900, 1045
Hpy188III TCNNGA 4 cut(s) 347, 460, 750, 987
Hpy8I GTNNAC 1 cut(s) 1026
Hpy99I CGWCG 2 cut(s) 1049, 1052
HpyAV CCTTC 2 cut(s) 474, 919
HpyCH4III ACNGT 4 cut(s) 27, 117, 515, 940
HpyCH4IV ACGT 3 cut(s) 111, 261, 866
HpyCH4V TGCA 6 cut(s) 408, 503, 567, 604, 643, 836
HpyF10VI GCNNNNNNNGC 2 cut(s) 414, 573
HpyF3I CTNAG 2 cut(s) 425, 807
HpySE526I ACGT 3 cut(s) 111, 261, 866
Hsp92II CATG 8 cut(s) 35, 225, 458, 463, 479, 503, 865, 1070
HspAI GCGC 1 cut(s) 839
Ksp22I TGATCA 2 cut(s) 492, 790
Kzo9I GATC 8 cut(s) 7, 96, 211, 358, 492, 649, 790, 874
LguI GCTCTTC 1 cut(s) 748
LpnPI CCDG 9 cut(s) 65, 289, 332, 334, 396, 405, 735, 772, 994
Lsp1109I GCAGC 5 cut(s) 655, 772, 790, 848, 898
LweI GCATC 2 cut(s) 591, 1011
MaeI CTAG 9 cut(s) 12, 312, 614, 626, 797, 915, 987, 1020, 1086
MaeII ACGT 3 cut(s) 111, 261, 866
MaeIII GTNAC 2 cut(s) 117, 884
MalI GATC 8 cut(s) 9, 98, 213, 360, 494, 651, 792, 876
MboI GATC 8 cut(s) 7, 96, 211, 358, 492, 649, 790, 874
MboII GAAGA 5 cut(s) 522, 655, 765, 869, 887
MflI RGATCY 2 cut(s) 7, 649
MluCI AATT 5 cut(s) 162, 464, 504, 593, 745
MlyI GAGTC 3 cut(s) 135, 681, 1049
MmeI TCCRAC 5 cut(s) 189, 558, 664, 849, 1068
MseI TTAA 3 cut(s) 69, 402, 971
MslI CAYNNNNRTG 1 cut(s) 444
MspA1I CMGCKG 1 cut(s) 785
MspI CCGG 3 cut(s) 276, 392, 759
MwoI GCNNNNNNNGC 2 cut(s) 414, 573
NdeII GATC 8 cut(s) 7, 96, 211, 358, 492, 649, 790, 874
NlaIII CATG 8 cut(s) 35, 225, 458, 463, 479, 503, 865, 1070
NlaIV GGNNCC 2 cut(s) 280, 396
NspV TTCGAA 1 cut(s) 997
PaeR7I CTCGAG 1 cut(s) 689
PagI TCATGA 1 cut(s) 459
PciSI GCTCTTC 1 cut(s) 748
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PfeI GAWTC 7 cut(s) 83, 183, 266, 299, 343, 901, 994
Pfl23II CGTACG 1 cut(s) 39
PflMI CCANNNNNTGG 1 cut(s) 193
PinAI ACCGGT 1 cut(s) 391
PkrI GCNGC 7 cut(s) 369, 645, 787, 805, 838, 913, 959
PleI GAGTC 3 cut(s) 135, 681, 1048
PpsI GAGTC 3 cut(s) 135, 681, 1048
PspLI CGTACG 1 cut(s) 39
PspN4I GGNNCC 2 cut(s) 280, 396
PspPI GGNCC 3 cut(s) 278, 673, 981
PspXI VCTCGAGB 1 cut(s) 689
PstI CTGCAG 1 cut(s) 410
PsuI RGATCY 2 cut(s) 7, 649
RsaI GTAC 6 cut(s) 24, 29, 41, 114, 310, 608
RsaNI GTAC 6 cut(s) 23, 28, 40, 113, 309, 607
RseI CAYNNNNRTG 1 cut(s) 444
SapI GCTCTTC 1 cut(s) 748
SaqAI TTAA 3 cut(s) 69, 402, 971
SatI GCNGC 7 cut(s) 368, 644, 786, 804, 837, 912, 958
Sau3AI GATC 8 cut(s) 7, 96, 211, 358, 492, 649, 790, 874
Sau96I GGNCC 3 cut(s) 278, 673, 981
ScaI AGTACT 1 cut(s) 608
SchI GAGTC 3 cut(s) 135, 681, 1049
SfaNI GCATC 2 cut(s) 591, 1011
SfcI CTRYAG 1 cut(s) 406
Sfr274I CTCGAG 1 cut(s) 689
SfuI TTCGAA 1 cut(s) 997
SinI GGWCC 1 cut(s) 278
SlaI CTCGAG 1 cut(s) 689
SmiMI CAYNNNNRTG 1 cut(s) 444
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
SpeI ACTAGT 1 cut(s) 311
Sse9I AATT 5 cut(s) 162, 464, 504, 593, 745
SsiI CCGC 5 cut(s) 122, 252, 368, 783, 958
SspMI CTAG 9 cut(s) 12, 312, 614, 626, 797, 915, 987, 1020, 1086
StyI CCWWGG 1 cut(s) 11
TaaI ACNGT 4 cut(s) 27, 117, 515, 940
TaiI ACGT 3 cut(s) 114, 264, 869
TaqI TCGA 5 cut(s) 102, 302, 519, 690, 997
TasI AATT 5 cut(s) 162, 464, 504, 593, 745
TatI WGTACW 1 cut(s) 606
TauI GCSGC 2 cut(s) 370, 960
TfiI GAWTC 7 cut(s) 83, 183, 266, 299, 343, 901, 994
Tru1I TTAA 3 cut(s) 69, 402, 971
Tru9I TTAA 3 cut(s) 69, 402, 971
TscAI CASTG 1 cut(s) 245
TseI GCWGC 5 cut(s) 643, 785, 803, 836, 911
TspDTI ATGAA 4 cut(s) 464, 476, 539, 1055
TspGWI ACGGA 1 cut(s) 981
TspRI CASTG 1 cut(s) 245
Van91I CCANNNNNTGG 1 cut(s) 193
VpaK11BI GGWCC 1 cut(s) 278
XagI CCTNNNNNAGG 1 cut(s) 321
XapI RAATTY 2 cut(s) 464, 745
XbaI TCTAGA 1 cut(s) 986
XhoI CTCGAG 1 cut(s) 689
XmaJI CCTAGG 1 cut(s) 11
XspI CTAG 9 cut(s) 12, 312, 614, 626, 797, 915, 987, 1020, 1086
ZrmI AGTACT 1 cut(s) 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.