FvH4_7g08730

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006657
Physical Location & Seq
Forward (+)
8557494 .. 8558534
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g08730.t1

Sequence Viewer

Length: 525 bp
ATGGAGGATGAAGATGAGTGGGAAGAAGTTTTAAGCTATCCTGAAGAATTCATCTATGAGGTGTTTGATATTGAAGACTTCGGTCCTCCAAATTCAAATGCAATGCAGGGCCTACATGTCGAAAAGGCGTCGTCATCCAAAGGGAAAGGGAAAATGAGGGGTCGCCCTCCCAAGCTTCATAGGTTTTACCTTGCAGAACAGAGGGAGAGAGCGGCGAAAATGAAACTGGATGCGCAGAGGGAGAGAGCGGCGAGCATGAAATTGGAGGATCTTCGTGTTTACTTTCATCTCAGCATTGAAGAAGCTTCTGAGAAATTGCGGCTTCCCATAGCTATTGTCAAAAGGCTCTGCCATAAGTTTGGAGTATGCAGGTGGCCTAGTAGAAAGCAGCTCAAAGCTATTTCAAAGGAGATTTCAAGTCTACAACCTATGTTGTTGTCATATAGGAAGGGCACCAGAGATTATATGGCAGCTAGAATTGAAAGTTTGGAGAAGGAGAGAGCAATCCTTTTGCATTATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

175

Amino Acids

20.39

Weight (kDa)

8.98

Isoelectric Point (pI)

60.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 85 - 129 6.5e-14 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 360
Acc16I TGCGCA 1 cut(s) 234
Acc36I ACCTGC 1 cut(s) 360
AccB1I GGYRCC 1 cut(s) 452
AccBSI CCGCTC 2 cut(s) 212, 248
AccI GTMKAC 1 cut(s) 421
AciI CCGC 3 cut(s) 212, 248, 319
AclWI GGATC 1 cut(s) 276
AcsI RAATTY 2 cut(s) 47, 91
AcuI CTGAAG 1 cut(s) 63
AcyI GRCGYC 1 cut(s) 128
AflIII ACRYGT 1 cut(s) 115
AgsI TTSAA 6 cut(s) 74, 96, 299, 405, 417, 482
AluBI AGCT 8 cut(s) 36, 175, 305, 332, 391, 398, 473, 522
AluI AGCT 8 cut(s) 36, 175, 305, 332, 391, 398, 473, 522
AlwI GGATC 1 cut(s) 276
AoxI GGCC 2 cut(s) 109, 374
ApeKI GCWGC 2 cut(s) 388, 470
ApoI RAATTY 2 cut(s) 47, 91
AspLEI GCGC 1 cut(s) 235
AspS9I GGNCC 2 cut(s) 83, 109
AvaII GGWCC 1 cut(s) 83
BaeGI GKGCMC 1 cut(s) 455
BanI GGYRCC 1 cut(s) 452
BbsI GAAGAC 1 cut(s) 81
BbvI GCAGC 2 cut(s) 400, 482
BfaI CTAG 2 cut(s) 378, 474
BfuAI ACCTGC 1 cut(s) 360
BisI GCNGC 5 cut(s) 213, 249, 320, 389, 471
BlsI GCNGC 5 cut(s) 214, 250, 321, 390, 472
Bme18I GGWCC 1 cut(s) 83
BmgT120I GGNCC 2 cut(s) 83, 109
BmiI GGNNCC 1 cut(s) 454
BmsI GCATC 1 cut(s) 220
BoxI GACNNNNGTC 1 cut(s) 81
BpiI GAAGAC 1 cut(s) 81
BsaHI GRCGYC 1 cut(s) 128
Bse1I ACTGG 1 cut(s) 231
Bse3DI GCAATG 1 cut(s) 108
BseGI GGATG 3 cut(s) 13, 134, 235
BseMI GCAATG 1 cut(s) 108
BseMII CTCAG 2 cut(s) 300, 304
BseNI ACTGG 1 cut(s) 231
BseSI GKGCMC 1 cut(s) 455
BseXI GCAGC 2 cut(s) 400, 482
BshFI GGCC 2 cut(s) 111, 376
BshNI GGYRCC 1 cut(s) 452
BsnI GGCC 2 cut(s) 111, 376
Bsp1286I GDGCHC 1 cut(s) 455
Bsp143I GATC 1 cut(s) 268
BspACI CCGC 3 cut(s) 212, 248, 319
BspANI GGCC 2 cut(s) 111, 376
BspCNI CTCAG 2 cut(s) 301, 303
BspLI GGNNCC 1 cut(s) 454
BspMI ACCTGC 1 cut(s) 360
BspPI GGATC 1 cut(s) 276
BspT107I GGYRCC 1 cut(s) 452
BsrBI CCGCTC 2 cut(s) 212, 248
BsrDI GCAATG 1 cut(s) 108
BsrI ACTGG 1 cut(s) 231
BssMI GATC 1 cut(s) 268
BssNI GRCGYC 1 cut(s) 128
BstACI GRCGYC 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 2 cut(s) 290, 309
BstF5I GGATG 3 cut(s) 13, 134, 235
BstHHI GCGC 1 cut(s) 235
BstKTI GATC 1 cut(s) 271
BstMBI GATC 1 cut(s) 268
BstNSI RCATGY 1 cut(s) 119
BstPAI GACNNNNGTC 1 cut(s) 81
BstSLI GKGCMC 1 cut(s) 455
BstV1I GCAGC 2 cut(s) 400, 482
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 1 cut(s) 268
BstXI CCANNNNNNTGG 1 cut(s) 359
BstYI RGATCY 1 cut(s) 268
BsuRI GGCC 2 cut(s) 111, 376
BtsCI GGATG 3 cut(s) 13, 134, 235
BveI ACCTGC 1 cut(s) 360
Cac8I GCNNGC 1 cut(s) 253
CfoI GCGC 1 cut(s) 235
Cfr13I GGNCC 2 cut(s) 83, 109
CseI GACGC 1 cut(s) 117
CviAII CATG 2 cut(s) 116, 256
DdeI CTNAG 2 cut(s) 290, 309
DpnI GATC 1 cut(s) 270
DpnII GATC 1 cut(s) 268
Eco47I GGWCC 1 cut(s) 83
Eco57I CTGAAG 1 cut(s) 63
EcoO109I RGGNCCY 1 cut(s) 109
EcoRI GAATTC 1 cut(s) 47
FaeI CATG 2 cut(s) 119, 259
FatI CATG 2 cut(s) 115, 255
FblI GTMKAC 1 cut(s) 421
Fnu4HI GCNGC 5 cut(s) 213, 249, 320, 389, 471
FokI GGATG 3 cut(s) 20, 121, 242
Fsp4HI GCNGC 5 cut(s) 213, 249, 320, 389, 471
FspBI CTAG 2 cut(s) 378, 474
FspI TGCGCA 1 cut(s) 234
GlaI GCGC 1 cut(s) 234
GluI GCNGC 5 cut(s) 213, 249, 320, 389, 471
HaeIII GGCC 2 cut(s) 111, 376
HgaI GACGC 1 cut(s) 117
HhaI GCGC 1 cut(s) 235
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 2 cut(s) 119, 259
Hin6I GCGC 1 cut(s) 233
HinP1I GCGC 1 cut(s) 233
HindIII AAGCTT 2 cut(s) 173, 303
Hpy166II GTNNAC 2 cut(s) 280, 422
Hpy188I TCNGA 1 cut(s) 310
Hpy188III TCNNGA 1 cut(s) 41
Hpy8I GTNNAC 2 cut(s) 280, 422
Hpy99I CGWCG 1 cut(s) 133
HpyAV CCTTC 2 cut(s) 442, 487
HpyCH4V TGCA 5 cut(s) 101, 106, 194, 369, 514
HpyF3I CTNAG 2 cut(s) 290, 309
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 2 cut(s) 119, 259
HspAI GCGC 1 cut(s) 233
Kzo9I GATC 1 cut(s) 268
LpnPI CCDG 5 cut(s) 54, 92, 212, 355, 469
Lsp1109I GCAGC 2 cut(s) 400, 482
LweI GCATC 1 cut(s) 220
MaeI CTAG 2 cut(s) 378, 474
MalI GATC 1 cut(s) 270
MbiI CCGCTC 2 cut(s) 212, 248
MboI GATC 1 cut(s) 268
MboII GAAGA 6 cut(s) 23, 35, 56, 86, 263, 311
MflI RGATCY 1 cut(s) 268
MhlI GDGCHC 1 cut(s) 455
MluCI AATT 5 cut(s) 47, 91, 260, 314, 477
MnlI CCTC 7 cut(s) 52, 96, 150, 177, 195, 231, 259
MseI TTAA 1 cut(s) 32
NdeII GATC 1 cut(s) 268
NlaIII CATG 2 cut(s) 119, 259
NlaIV GGNNCC 1 cut(s) 454
NsbI TGCGCA 1 cut(s) 234
NspI RCATGY 1 cut(s) 119
PaqCI CACCTGC 1 cut(s) 360
PciI ACATGT 1 cut(s) 115
PkrI GCNGC 5 cut(s) 214, 250, 321, 390, 472
PscI ACATGT 1 cut(s) 115
PshAI GACNNNNGTC 1 cut(s) 81
PspN4I GGNNCC 1 cut(s) 454
PspPI GGNCC 2 cut(s) 83, 109
PsuI RGATCY 1 cut(s) 268
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 5 cut(s) 213, 249, 320, 389, 471
Sau3AI GATC 1 cut(s) 268
Sau96I GGNCC 2 cut(s) 83, 109
SduI GDGCHC 1 cut(s) 455
SfaNI GCATC 1 cut(s) 220
SinI GGWCC 1 cut(s) 83
Sse9I AATT 5 cut(s) 47, 91, 260, 314, 477
SsiI CCGC 3 cut(s) 212, 248, 319
SspMI CTAG 2 cut(s) 378, 474
TaqI TCGA 1 cut(s) 120
TaqII GACCGA 1 cut(s) 71
TasI AATT 5 cut(s) 47, 91, 260, 314, 477
TauI GCSGC 3 cut(s) 215, 251, 322
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TseI GCWGC 2 cut(s) 388, 470
TspDTI ATGAA 6 cut(s) 24, 40, 167, 236, 272, 275
VpaK11BI GGWCC 1 cut(s) 83
XapI RAATTY 2 cut(s) 47, 91
XceI RCATGY 1 cut(s) 119
XcmI CCANNNNNNNNNTGG 1 cut(s) 463
XmiI GTMKAC 1 cut(s) 421
XspI CTAG 2 cut(s) 378, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.