Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004691

Physical Location & Seq

Genomic Coordinates Forward (+)

9690428 .. 9693289

Gene Structure 2862 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G27450.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1137 bp

ATGGAAGTGAAAGCTAGAGCTCCTGGGAAGATCATACTTGCAGGGGAACACGCTGTTGTTCATGGATCCACCGCTGTAGCTGCCGCCATTGATCTCTACACTTACGTTACTCTCCGCTTTCCTCTTCCATCAGCTGAGAACAATGATAGGCTTACACTTCAGCTCAAGGACATTTCCTTGGAGTTTTCATGGTCCTTAGCCAGAATCAAAGAAGCGATTCCTTATGATTCAAGCACTCTCTGCCGTTCTACGCCGGCTTCATGTTCAGAGGAGACCCTTAAATCAATTGCAGTTTTGGTTGAAGAGCAAAATCTTCCAAAGGAAAAGATGTGGCTCTCCTCTGGGATCTCCACGTTTCTCTGGTTATACACCAGAATTATAGGGTTCAATCCGGCTACAGTAGTCATTAACTCTGAGCTTCCATACGGGTCTGGCCTCGGTTCATCAGCAGCTTTATGTGTAGCTCTCACAGCTGCTCTCCTTGCTTCTTCTATTTCAGAGAAAACCCGTGGTAACGGTTGGTCATCTCTCGATGAAACCAATCTTGAGTTGCTAAATAAATGGGCTTTCGAAGGCGAAAAGATCATCCATGGGAAACCTTCTGGGATAGACAACACCGTCAGTGCATACGGCAACATGATCAAGTTCTGCTCAGGCGAGATAACTCGGTTACAATCCAACATGCCTCTGAGAATGCTAATTACCAACACTAGAGTTGGGCGAAACACAAAAGCTCTGGTCTCTGGTGTGTCACAGAGAGCGGTAAGACATCCTGATGCGATGAAGTCAGTGTTCAACGCCGTGGATTCTATAAGCAAAGAGCTCGCTGCGATCATTCAGTCTAAAGACGAGACCTCAGTTACAGAAAAAGAAGAGAGAATAAAAGAACTCATGGAGATGAACCAAGGTCTGCTCCTGTCAATGGGGGTTAGCCACAGCTCAATCGAGGCTGTGATTCTAACCACGGTCAAGCACAAGCTTGTCTCCAAACTTACAGGAGCTGGTGGCGGCGGCTGCGTCCTCACTCTATTACCAACCGGGACGGTGGTGGACAAAGTGGTGGAGGAGCTCGAGTCCAGCGGTTTTCAGTGTTTCACGGCATTGATTGGTGGTAACGGAGCTCAGATTTGCTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000287 GO:0003674 GO:0003824 GO:0004496 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006084 GO:0006139 GO:0006163 GO:0006629 GO:0006637 GO:0006644 GO:0006720 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008202 GO:0008299 GO:0008610 GO:0008654 GO:0009058 GO:0009117 GO:0009150 GO:0009240 GO:0009259 GO:0009987 GO:0016125 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019287 GO:0019637 GO:0019693 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0035383 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043169 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046490 GO:0046872 GO:0051186 GO:0055086 GO:0071704 GO:0072521 GO:0090407 GO:0097159 GO:0097367 GO:1901135 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00900 ko01100 ko01110 ko01130 ko04146 map00900 map01100 map01110 map01130 map04146

Pfam Domains

Protein Families

GHMP_kinases_C GHMP_kinases_N

Protein Analysis

378

Amino Acids

40.64

Weight (kDa)

6.35

Isoelectric Point (pI)

31.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GHMP_kinases_N	PF00288	126 - 211	7.3e-11	GHMP kinases N terminal domain
GHMP_kinases_C	PF08544	292 - 359	1.5e-06	GHMP kinases C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004691)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G27450 AT5G27450 AT5G27450
fragaria_vesca	FvH4_6g05650 FvH4_6g05650
malus_domestica	MD04G1206900.v1.1 MD12G1220700.v1.1
prunus_persica	Prupe.6G327600_v2.0.a1
pyrus_communis	pycom04g18290 pycom12g20650
rosa_chinensis	RchiOBHm_Chr3g0454501 RchiOBHm_Chr7g0220931
rosa_laevigata	RLG00000002188 RLG00000002192 RLG00000025439
rosa_multiflora	Rmu_sc0002105.1_g000027 Rmu_sc0007209.1_g000003 Rmu_sc0026738.1_g000003
rosa_roxburghii	Rroxscaffold_6G00425020 Rroxscaffold_6G00425040
rosa_rugosa	Rorug03G0000100 Rorug07G0198300
rosa_samantha	Rh3AG059300 Rh3BG061400 Rh3CG060200 Rh3DG061100 Rh7DG337500
rosa_wichuraiana	Rw0G017160 Rw0G017190 Rw3G004600 Rw7G028810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	617
AccBSI	CCGCTC	1 cut(s)	761
AciI	CCGC	7 cut(s)	72, 84, 115, 761, 1008, 1011, 1080
AclWI	GGATC	3 cut(s)	60, 73, 353
AcuI	CTGAAG	1 cut(s)	143
AfiI	CCNNNNNNNGG	1 cut(s)	922
AgsI	TTSAA	4 cut(s)	231, 302, 388, 796
AjnI	CCWGG	1 cut(s)	22
Alw21I	GWGCWC	4 cut(s)	22, 825, 1071, 1123
Alw26I	GTCTC	4 cut(s)	266, 745, 845, 988
AlwI	GGATC	3 cut(s)	60, 73, 353
AlwNI	CAGNNNCTG	1 cut(s)	1001
Ama87I	CYCGRG	1 cut(s)	1070
AoxI	GGCC	1 cut(s)	433
ApeKI	GCWGC	5 cut(s)	80, 449, 473, 827, 1014
Asp700I	GAANNNNTTC	1 cut(s)	216
AspS9I	GGNCC	1 cut(s)	192
AsuC2I	CCSGG	1 cut(s)	1039
AsuII	TTCGAA	1 cut(s)	570
AvaI	CYCGRG	1 cut(s)	1070
AvaII	GGWCC	1 cut(s)	192
BamHI	GGATCC	1 cut(s)	65
BanII	GRGCYC	4 cut(s)	22, 825, 1071, 1123
Bbv12I	GWGCWC	4 cut(s)	22, 825, 1071, 1123
BbvI	GCAGC	5 cut(s)	67, 460, 461, 814, 1001
BccI	CCATC	1 cut(s)	136
BceAI	ACGGC	4 cut(s)	228, 646, 785, 1113
BcgI	CGANNNNNNTGC	2 cut(s)	805, 839
BciT130I	CCWGG	1 cut(s)	24
BclI	TGATCA	1 cut(s)	639
BcnI	CCSGG	1 cut(s)	1039
BcoDI	GTCTC	4 cut(s)	266, 745, 845, 988
BfaI	CTAG	2 cut(s)	15, 711
BfmI	CTRYAG	2 cut(s)	75, 396
BisI	GCNGC	8 cut(s)	81, 84, 450, 474, 828, 1009, 1012, 1015
BlsI	GCNGC	8 cut(s)	82, 85, 451, 475, 829, 1010, 1013, 1016
Bme1390I	CCNGG	2 cut(s)	24, 1039
Bme18I	GGWCC	1 cut(s)	192
BmeT110I	CYCGRG	1 cut(s)	1070
BmgT120I	GGNCC	1 cut(s)	192
BmiI	GGNNCC	1 cut(s)	67
BmrFI	CCNGG	2 cut(s)	24, 1039
BmsI	GCATC	1 cut(s)	766
Bpu10I	CCTNAGC	2 cut(s)	196, 652
Bpu14I	TTCGAA	1 cut(s)	570
BpuEI	CTTGAG	2 cut(s)	149, 566
BpuMI	CCSGG	1 cut(s)	1039
BsaI	GGTCTC	3 cut(s)	266, 745, 845
BsaJI	CCNNGG	8 cut(s)	23, 177, 436, 508, 589, 801, 904, 963
BsaXI	ACNNNNNCTCC	2 cut(s)	1058, 1088
Bsc4I	CCNNNNNNNGG	1 cut(s)	922
Bse118I	RCCGGY	1 cut(s)	253
BseBI	CCWGG	1 cut(s)	24
BseDI	CCNNGG	8 cut(s)	23, 177, 436, 508, 589, 801, 904, 963
BseGI	GGATG	2 cut(s)	585, 769
BseLI	CCNNNNNNNGG	1 cut(s)	922
BseMII	CTCAG	6 cut(s)	126, 405, 666, 680, 870, 1136
BseRI	GAGGAG	3 cut(s)	284, 328, 1079
BseXI	GCAGC	5 cut(s)	67, 460, 461, 814, 1001
BshFI	GGCC	1 cut(s)	435
BsiHKAI	GWGCWC	4 cut(s)	22, 825, 1071, 1123
BsiHKCI	CYCGRG	1 cut(s)	1070
BsiSI	CCGG	3 cut(s)	254, 392, 1038
BslFI	GGGAC	1 cut(s)	1054
BslI	CCNNNNNNNGG	1 cut(s)	922
BsmAI	GTCTC	4 cut(s)	266, 745, 845, 988
BsmFI	GGGAC	1 cut(s)	1054
BsmI	GAATGC	1 cut(s)	699
BsnI	GGCC	1 cut(s)	435
Bso31I	GGTCTC	3 cut(s)	266, 745, 845
BsoBI	CYCGRG	1 cut(s)	1070
Bsp119I	TTCGAA	1 cut(s)	570
Bsp1286I	GDGCHC	4 cut(s)	22, 825, 1071, 1123
Bsp143I	GATC	7 cut(s)	30, 65, 91, 345, 582, 639, 831
Bsp19I	CCATGG	1 cut(s)	589
BspACI	CCGC	7 cut(s)	72, 84, 115, 761, 1008, 1011, 1080
BspANI	GGCC	1 cut(s)	435
BspCNI	CTCAG	6 cut(s)	127, 406, 665, 681, 869, 1135
BspLI	GGNNCC	1 cut(s)	67
BspPI	GGATC	3 cut(s)	60, 73, 353
BspQI	GCTCTTC	1 cut(s)	297
BspT104I	TTCGAA	1 cut(s)	570
BspTNI	GGTCTC	3 cut(s)	266, 745, 845
BsrBI	CCGCTC	1 cut(s)	761
BsrFI	RCCGGY	1 cut(s)	253
BssAI	RCCGGY	1 cut(s)	253
BssECI	CCNNGG	8 cut(s)	23, 177, 436, 508, 589, 801, 904, 963
BssMI	GATC	7 cut(s)	30, 65, 91, 345, 582, 639, 831
BssT1I	CCWWGG	3 cut(s)	177, 589, 904
Bst2UI	CCWGG	1 cut(s)	24
Bst4CI	ACNGT	5 cut(s)	400, 518, 619, 967, 1045
Bst6I	CTCTTC	3 cut(s)	129, 297, 867
BstAPI	GCANNNNNTGC	1 cut(s)	240
BstBI	TTCGAA	1 cut(s)	570
BstC8I	GCNNGC	2 cut(s)	255, 825
BstDEI	CTNAG	7 cut(s)	135, 196, 414, 652, 689, 856, 1122
BstDSI	CCRYGG	4 cut(s)	508, 589, 801, 963
BstF5I	GGATG	2 cut(s)	585, 769
BstKTI	GATC	7 cut(s)	33, 68, 94, 348, 585, 642, 834
BstMAI	GTCTC	4 cut(s)	266, 745, 845, 988
BstMBI	GATC	7 cut(s)	30, 65, 91, 345, 582, 639, 831
BstMWI	GCNNNNNNNGC	5 cut(s)	80, 240, 470, 482, 1014
BstNI	CCWGG	1 cut(s)	24
BstNSI	RCATGY	1 cut(s)	685
BstSCI	CCNGG	2 cut(s)	22, 1037
BstSFI	CTRYAG	2 cut(s)	75, 396
BstV1I	GCAGC	5 cut(s)	67, 460, 461, 814, 1001
BstX2I	RGATCY	2 cut(s)	65, 345
BstYI	RGATCY	2 cut(s)	65, 345
BsuRI	GGCC	1 cut(s)	435
BtgI	CCRYGG	4 cut(s)	508, 589, 801, 963
BtgZI	GCGATG	1 cut(s)	794
BtsCI	GGATG	2 cut(s)	585, 769
BtsIMutI	CAGTG	3 cut(s)	628, 795, 1094
Cac8I	GCNNGC	2 cut(s)	255, 825
CaiI	CAGNNNCTG	1 cut(s)	1001
Cfr10I	RCCGGY	1 cut(s)	253
Cfr13I	GGNCC	1 cut(s)	192
CseI	GACGC	1 cut(s)	1006
CviAII	CATG	7 cut(s)	62, 189, 261, 590, 637, 682, 892
DdeI	CTNAG	7 cut(s)	135, 196, 414, 652, 689, 856, 1122
DpnI	GATC	7 cut(s)	32, 67, 93, 347, 584, 641, 833
DpnII	GATC	7 cut(s)	30, 65, 91, 345, 582, 639, 831
DrdI	GACNNNNNNGTC	1 cut(s)	617
DseDI	GACNNNNNNGTC	1 cut(s)	617
Eam1104I	CTCTTC	3 cut(s)	129, 297, 867
EarI	CTCTTC	3 cut(s)	129, 297, 867
Ecl136II	GAGCTC	4 cut(s)	20, 823, 1069, 1121
Eco130I	CCWWGG	3 cut(s)	177, 589, 904
Eco24I	GRGCYC	4 cut(s)	22, 825, 1071, 1123
Eco31I	GGTCTC	3 cut(s)	266, 745, 845
Eco47I	GGWCC	1 cut(s)	192
Eco53kI	GAGCTC	4 cut(s)	20, 823, 1069, 1121
Eco57I	CTGAAG	1 cut(s)	143
Eco88I	CYCGRG	1 cut(s)	1070
EcoICRI	GAGCTC	4 cut(s)	20, 823, 1069, 1121
EcoRII	CCWGG	1 cut(s)	22
EcoT14I	CCWWGG	3 cut(s)	177, 589, 904
EcoT38I	GRGCYC	4 cut(s)	22, 825, 1071, 1123
ErhI	CCWWGG	3 cut(s)	177, 589, 904
FaeI	CATG	7 cut(s)	65, 192, 264, 593, 640, 685, 895
FaqI	GGGAC	1 cut(s)	1054
FatI	CATG	7 cut(s)	61, 188, 260, 589, 636, 681, 891
FbaI	TGATCA	1 cut(s)	639
Fnu4HI	GCNGC	8 cut(s)	81, 84, 450, 474, 828, 1009, 1012, 1015
FokI	GGATG	2 cut(s)	572, 756
FriOI	GRGCYC	4 cut(s)	22, 825, 1071, 1123
Fsp4HI	GCNGC	8 cut(s)	81, 84, 450, 474, 828, 1009, 1012, 1015
FspBI	CTAG	2 cut(s)	15, 711
GluI	GCNGC	8 cut(s)	81, 84, 450, 474, 828, 1009, 1012, 1015
HaeIII	GGCC	1 cut(s)	435
HapII	CCGG	3 cut(s)	254, 392, 1038
HgaI	GACGC	1 cut(s)	1006
Hin1II	CATG	7 cut(s)	65, 192, 264, 593, 640, 685, 895
HindIII	AAGCTT	1 cut(s)	977
HinfI	GANTC	6 cut(s)	204, 217, 227, 806, 955, 1073
HpaII	CCGG	3 cut(s)	254, 392, 1038
Hpy166II	GTNNAC	1 cut(s)	1051
Hpy188I	TCNGA	5 cut(s)	268, 415, 499, 690, 1125
Hpy188III	TCNNGA	3 cut(s)	530, 545, 773
Hpy8I	GTNNAC	1 cut(s)	1051
HpyAV	CCTTC	2 cut(s)	566, 609
HpyCH4III	ACNGT	5 cut(s)	400, 518, 619, 967, 1045
HpyCH4IV	ACGT	2 cut(s)	105, 353
HpyCH4V	TGCA	3 cut(s)	41, 290, 626
HpyF10VI	GCNNNNNNNGC	5 cut(s)	80, 240, 470, 482, 1014
HpyF3I	CTNAG	7 cut(s)	135, 196, 414, 652, 689, 856, 1122
HpySE526I	ACGT	2 cut(s)	105, 353
Hsp92II	CATG	7 cut(s)	65, 192, 264, 593, 640, 685, 895
KroI	GCCGGC	1 cut(s)	253
KroNI	GCCGGC	1 cut(s)	255
Ksp22I	TGATCA	1 cut(s)	639
Kzo9I	GATC	7 cut(s)	30, 65, 91, 345, 582, 639, 831
LguI	GCTCTTC	1 cut(s)	297
LmnI	GCTCC	5 cut(s)	25, 918, 998, 1066, 1118
Lsp1109I	GCAGC	5 cut(s)	67, 460, 461, 814, 1001
LweI	GCATC	1 cut(s)	766
MaeI	CTAG	2 cut(s)	15, 711
MaeII	ACGT	2 cut(s)	105, 353
MaeIII	GTNAC	6 cut(s)	106, 512, 669, 750, 859, 1112
MalI	GATC	7 cut(s)	32, 67, 93, 347, 584, 641, 833
MbiI	CCGCTC	1 cut(s)	761
MboI	GATC	7 cut(s)	30, 65, 91, 345, 582, 639, 831
MboII	GAAGA	6 cut(s)	40, 116, 305, 314, 480, 884
MfeI	CAATTG	1 cut(s)	285
MflI	RGATCY	2 cut(s)	65, 345
MhlI	GDGCHC	4 cut(s)	22, 825, 1071, 1123
MluCI	AATT	3 cut(s)	285, 375, 699
MlyI	GAGTC	1 cut(s)	1082
MmeI	TCCRAC	1 cut(s)	702
MnlI	CCTC	9 cut(s)	132, 262, 349, 446, 696, 865, 940, 1031, 1057
MroNI	GCCGGC	1 cut(s)	253
MroXI	GAANNNNTTC	1 cut(s)	216
MseI	TTAA	2 cut(s)	279, 408
MslI	CAYNNNNRTG	2 cut(s)	774, 896
MspA1I	CMGCKG	4 cut(s)	74, 134, 473, 1080
MspI	CCGG	3 cut(s)	254, 392, 1038
MspR9I	CCNGG	2 cut(s)	24, 1039
MunI	CAATTG	1 cut(s)	285
Mva1269I	GAATGC	1 cut(s)	699
MvaI	CCWGG	1 cut(s)	24
MwoI	GCNNNNNNNGC	5 cut(s)	80, 240, 470, 482, 1014
NaeI	GCCGGC	1 cut(s)	255
NciI	CCSGG	1 cut(s)	1039
NcoI	CCATGG	1 cut(s)	589
NdeII	GATC	7 cut(s)	30, 65, 91, 345, 582, 639, 831
NgoMIV	GCCGGC	1 cut(s)	253
NlaIII	CATG	7 cut(s)	65, 192, 264, 593, 640, 685, 895
NlaIV	GGNNCC	1 cut(s)	67
NmuCI	GTSAC	1 cut(s)	750
NspI	RCATGY	1 cut(s)	685
NspV	TTCGAA	1 cut(s)	570
PaeR7I	CTCGAG	1 cut(s)	1070
PciSI	GCTCTTC	1 cut(s)	297
PctI	GAATGC	1 cut(s)	699
PdiI	GCCGGC	1 cut(s)	255
PdmI	GAANNNNTTC	1 cut(s)	216
PfeI	GAWTC	5 cut(s)	204, 217, 227, 806, 955
PkrI	GCNGC	8 cut(s)	82, 85, 451, 475, 829, 1010, 1013, 1016
PleI	GAGTC	1 cut(s)	1081
PpsI	GAGTC	1 cut(s)	1081
Psp124BI	GAGCTC	4 cut(s)	22, 825, 1071, 1123
Psp6I	CCWGG	1 cut(s)	22
PspGI	CCWGG	1 cut(s)	22
PspN4I	GGNNCC	1 cut(s)	67
PspPI	GGNCC	1 cut(s)	192
PspXI	VCTCGAGB	1 cut(s)	1070
PstNI	CAGNNNCTG	1 cut(s)	1001
PsuI	RGATCY	2 cut(s)	65, 345
PvuII	CAGCTG	2 cut(s)	134, 473
RseI	CAYNNNNRTG	2 cut(s)	774, 896
SacI	GAGCTC	4 cut(s)	22, 825, 1071, 1123
SapI	GCTCTTC	1 cut(s)	297
SaqAI	TTAA	2 cut(s)	279, 408
SatI	GCNGC	8 cut(s)	81, 84, 450, 474, 828, 1009, 1012, 1015
Sau3AI	GATC	7 cut(s)	30, 65, 91, 345, 582, 639, 831
Sau96I	GGNCC	1 cut(s)	192
SchI	GAGTC	1 cut(s)	1082
ScrFI	CCNGG	2 cut(s)	24, 1039
SduI	GDGCHC	4 cut(s)	22, 825, 1071, 1123
SfaNI	GCATC	1 cut(s)	766
SfcI	CTRYAG	2 cut(s)	75, 396
Sfr274I	CTCGAG	1 cut(s)	1070
SfuI	TTCGAA	1 cut(s)	570
SinI	GGWCC	1 cut(s)	192
SlaI	CTCGAG	1 cut(s)	1070
SmiMI	CAYNNNNRTG	2 cut(s)	774, 896
SmlI	CTYRAG	3 cut(s)	164, 545, 1070
SmoI	CTYRAG	3 cut(s)	164, 545, 1070
Sse9I	AATT	3 cut(s)	285, 375, 699
SsiI	CCGC	7 cut(s)	72, 84, 115, 761, 1008, 1011, 1080
SspMI	CTAG	2 cut(s)	15, 711
SstI	GAGCTC	4 cut(s)	22, 825, 1071, 1123
StyD4I	CCNGG	2 cut(s)	22, 1037
StyI	CCWWGG	3 cut(s)	177, 589, 904
TaaI	ACNGT	5 cut(s)	400, 518, 619, 967, 1045
TaiI	ACGT	2 cut(s)	108, 356
TaqI	TCGA	4 cut(s)	531, 570, 945, 1071
TasI	AATT	3 cut(s)	285, 375, 699
TauI	GCSGC	3 cut(s)	86, 1011, 1014
TfiI	GAWTC	5 cut(s)	204, 217, 227, 806, 955
Tru1I	TTAA	2 cut(s)	279, 408
Tru9I	TTAA	2 cut(s)	279, 408
TscAI	CASTG	3 cut(s)	628, 795, 1094
TseFI	GTSAC	1 cut(s)	750
TseI	GCWGC	5 cut(s)	80, 449, 473, 827, 1014
Tsp45I	GTSAC	1 cut(s)	750
TspDTI	ATGAA	7 cut(s)	50, 177, 249, 432, 549, 797, 914
TspGWI	ACGGA	1 cut(s)	1131
TspRI	CASTG	3 cut(s)	628, 795, 1094
VpaK11BI	GGWCC	1 cut(s)	192
XceI	RCATGY	1 cut(s)	685
XhoI	CTCGAG	1 cut(s)	1070
XmnI	GAANNNNTTC	1 cut(s)	216
XspI	CTAG	2 cut(s)	15, 711

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.