Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003420

Physical Location & Seq

Genomic Coordinates Reverse (-)

10862016 .. 10864946

Gene Structure 2931 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G28840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1134 bp

ATGGGAACTACCAATGGAACAGACTATGGAGCATACACATACAAGGAGCTAGAAAGAGAGCAATATTGGCCATCTGAGAATCTCAAGATATCAATAACAGGAGCTGGAGGTTTCATTGCATCTCACATTGCTCGTCGTTTGAAGCACGAAGGTCATTACGTGATTGCTTCTGACTGGAAAAAGAATGAACACATGACTGAAGACATGTTCTGTGATGAGTTCCATCTTGTTGATCTTAGGGTTATGGAGAATTGTCTCAAAGTTACTGAAGGAGTTGATCATGTTTTTAACTTAGCTGCTGATATGGGTGGTATGGGTTTTATCCAGAGTAATCACTCTGTGATTATGTATAATAATACTATGATTAGTTTCAATATGATTGAGGCTGCTAGGATCAATGGGATTAAGAGGTTCTTTTATGCTTCGAGTGCTTGTATCTATCCAGAGTTTAAGCAGTTGGAGACTACTAATGTGAGCTTGAAGGAGTCAGATGCTTGGCCTGCAGAGCCTCAAGATGCTTATGGTTTGGAGAAGCTTGCTACGGAGGAGTTGTGTAAGCATTACAACAAAGATTTTGGTATTGAGTGTCGAATTGGAAGGTTCCATAACATTTATGGTCCTTTTGGAACATGGAAAGGTGGAAGGGAGAAGGCTCCAGCTGCTTTCTGTAGGAAGGCTCAGACTTCCACTGATAGGTTTGAGATGTGGGGAGATGGGCTTCAGACCCGTTCTTTTACCTTTATCGATGAGTGTGTTGAAGGTGTACTCAGGTTGACAAAATCAGATTTCCGTGAGCCGGTGAACATCGGAAGCGATGAGATGGTGAGCATGAATGAGATGGCTGAGATGGTTCTCAGCTTTGAGGAAAAGAAGCTTCCAATTCACCACATTCCTGGCCCGGAAGGTGTTCGTGGTCGTAACTCAGACAACAATCTGATCAAAGAAAAGCTTGGTTGGGCTCCTAATATGAGATTGAAGGAGGGGCTTAGAATAACCTACTTCTGGATAAAGGAACAGATCGAGAAAGAGAAAGCAAAGGGAAGCGATGTGTCGCTTTACGGGTCATCAAAGGTGGTTGGAACTCAAGCACCGGTTCAGCTAGGCTCACTCCGCGCGGCTGATGGAAAAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0005996 GO:0006082 GO:0006732 GO:0006766 GO:0006767 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009110 GO:0009987 GO:0016051 GO:0016053 GO:0016853 GO:0016854 GO:0016857 GO:0019752 GO:0019852 GO:0019853 GO:0036094 GO:0042364 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046364 GO:0046394 GO:0047918 GO:0048037 GO:0050662 GO:0051186 GO:0051188 GO:0051287 GO:0071704 GO:0097159 GO:1901265 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00053 ko00520 ko01100 ko01110 map00053 map00520 map01100 map01110

Pfam Domains

Protein Families

Epimerase

Protein Analysis

377

Amino Acids

42.76

Weight (kDa)

5.85

Isoelectric Point (pI)

40.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	30 - 270	4.9e-45	NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd	PF16363	32 - 331	2.2e-25	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	32 - 190	3e-10	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10	PF13460	34 - 152	8.3e-06	NAD(P)H-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003420)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G28840 AT5G28840
fragaria_vesca	FvH4_6g02980
malus_domestica	MD04G1224100.v1.1 MD04G1224200.v1.1 MD12G1240900.v1.1
prunus_persica	Prupe.1G025100_v2.0.a1 Prupe.1G025100_v2.0.a1 Prupe.6G342800_v2.0.a1
pyrus_communis	pycom04g19750
rosa_chinensis	RchiOBHm_Chr3g0451161 RchiOBHm_Chr4g0388911
rosa_laevigata	RLG00000010008 RLG00000010011 RLG00000025681 RLG00000025682
rosa_multiflora	Rmu_co8299443.1_g000001 Rmu_sc0005486.1_g000010 Rmu_ssc0000028.1_g000003
rosa_roxburghii	Rroxscaffold_5G00335220 Rroxscaffold_6G00428630
rosa_rugosa	Rorug02G0632400 Rorug02G0632500 Rorug03G0321000 Rorug03G0321000 Rorug03G0321100
rosa_samantha	Rh3AG034900 Rh3BG035300 Rh3CG033800 Rh3CG033900 Rh3DG034900 Rh4BG018800 Rh4CG028600 Rh4DG019300
rosa_wichuraiana	Rw3G002560 Rw4G001840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	1113, 1115
AciI	CCGC	2 cut(s)	1111, 1115
AclWI	GGATC	1 cut(s)	401
AcoI	YGGCCR	1 cut(s)	68
AcuI	CTGAAG	3 cut(s)	219, 288, 704
AdeI	CACNNNGTG	1 cut(s)	340
AfaI	GTAC	1 cut(s)	765
AfiI	CCNNNNNNNGG	3 cut(s)	693, 796, 1002
AflIII	ACRYGT	1 cut(s)	204
AgeI	ACCGGT	1 cut(s)	1090
AgsI	TTSAA	5 cut(s)	142, 373, 481, 758, 976
AjnI	CCWGG	1 cut(s)	892
Alw26I	GTCTC	2 cut(s)	260, 455
AlwI	GGATC	1 cut(s)	401
AlwNI	CAGNNNCTG	1 cut(s)	104
AoxI	GGCC	3 cut(s)	68, 497, 895
ApeKI	GCWGC	3 cut(s)	296, 386, 659
AsiGI	ACCGGT	1 cut(s)	1090
Asp700I	GAANNNNTTC	1 cut(s)	906
AspLEI	GCGC	1 cut(s)	1115
AspS9I	GGNCC	2 cut(s)	617, 896
AsuC2I	CCSGG	1 cut(s)	899
AsuHPI	GGTGA	3 cut(s)	811, 835, 875
AvaII	GGWCC	1 cut(s)	617
BalI	TGGCCA	1 cut(s)	70
BanII	GRGCYC	1 cut(s)	961
BbsI	GAAGAC	1 cut(s)	207
BbvI	GCAGC	3 cut(s)	283, 373, 646
BccI	CCATC	7 cut(s)	79, 231, 707, 814, 832, 841, 1115
BciT130I	CCWGG	1 cut(s)	894
BclI	TGATCA	2 cut(s)	277, 936
BcnI	CCSGG	1 cut(s)	899
BcoDI	GTCTC	2 cut(s)	260, 455
BfaI	CTAG	3 cut(s)	50, 390, 1100
BfmI	CTRYAG	2 cut(s)	501, 667
BisI	GCNGC	4 cut(s)	297, 387, 660, 1116
BlsI	GCNGC	4 cut(s)	298, 388, 661, 1117
Bme1390I	CCNGG	2 cut(s)	894, 899
Bme18I	GGWCC	1 cut(s)	617
BmgT120I	GGNCC	2 cut(s)	617, 896
BmiI	GGNNCC	3 cut(s)	602, 654, 960
BmrFI	CCNGG	2 cut(s)	894, 899
BmsI	GCATC	3 cut(s)	128, 481, 505
BpiI	GAAGAC	1 cut(s)	207
BpmI	CTGGAG	2 cut(s)	126, 639
BpuEI	CTTGAG	3 cut(s)	68, 495, 1068
BpuMI	CCSGG	1 cut(s)	899
Bsa29I	ATCGAT	1 cut(s)	744
BsaAI	YACGTR	1 cut(s)	160
BsaWI	WCCGGW	1 cut(s)	1090
BsaXI	ACNNNNNCTCC	2 cut(s)	536, 566
Bsc4I	CCNNNNNNNGG	3 cut(s)	693, 796, 1002
Bse118I	RCCGGY	2 cut(s)	796, 1090
Bse1I	ACTGG	1 cut(s)	179
Bse3DI	GCAATG	2 cut(s)	114, 126
BseBI	CCWGG	1 cut(s)	894
BseCI	ATCGAT	1 cut(s)	744
BseLI	CCNNNNNNNGG	3 cut(s)	693, 796, 1002
BseMI	GCAATG	2 cut(s)	114, 126
BseMII	CTCAG	6 cut(s)	66, 692, 781, 834, 868, 936
BseNI	ACTGG	1 cut(s)	179
BseRI	GAGGAG	1 cut(s)	560
BseXI	GCAGC	3 cut(s)	283, 373, 646
Bsh1236I	CGCG	2 cut(s)	1113, 1115
BshFI	GGCC	3 cut(s)	70, 499, 897
BshTI	ACCGGT	1 cut(s)	1090
BshVI	ATCGAT	1 cut(s)	744
BsiSI	CCGG	3 cut(s)	797, 899, 1091
BslI	CCNNNNNNNGG	3 cut(s)	693, 796, 1002
BsmAI	GTCTC	2 cut(s)	260, 455
BsnI	GGCC	3 cut(s)	70, 499, 897
Bsp1286I	GDGCHC	1 cut(s)	961
Bsp143I	GATC	5 cut(s)	232, 277, 393, 936, 1017
BspACI	CCGC	2 cut(s)	1111, 1115
BspANI	GGCC	3 cut(s)	70, 499, 897
BspCNI	CTCAG	6 cut(s)	67, 691, 780, 835, 867, 935
BspDI	ATCGAT	1 cut(s)	744
BspFNI	CGCG	2 cut(s)	1113, 1115
BspLI	GGNNCC	3 cut(s)	602, 654, 960
BspMAI	CTGCAG	1 cut(s)	505
BspPI	GGATC	1 cut(s)	401
BsrDI	GCAATG	2 cut(s)	114, 126
BsrFI	RCCGGY	2 cut(s)	796, 1090
BsrI	ACTGG	1 cut(s)	179
BssAI	RCCGGY	2 cut(s)	796, 1090
BssMI	GATC	5 cut(s)	232, 277, 393, 936, 1017
Bst2UI	CCWGG	1 cut(s)	894
BstBAI	YACGTR	1 cut(s)	160
BstC8I	GCNNGC	2 cut(s)	501, 537
BstDEI	CTNAG	9 cut(s)	75, 236, 292, 678, 767, 843, 854, 922, 986
BstFNI	CGCG	2 cut(s)	1113, 1115
BstHHI	GCGC	1 cut(s)	1115
BstKTI	GATC	5 cut(s)	235, 280, 396, 939, 1020
BstMAI	GTCTC	2 cut(s)	260, 455
BstMBI	GATC	5 cut(s)	232, 277, 393, 936, 1017
BstMWI	GCNNNNNNNGC	6 cut(s)	67, 428, 500, 505, 659, 1110
BstNI	CCWGG	1 cut(s)	894
BstNSI	RCATGY	1 cut(s)	208
BstSCI	CCNGG	2 cut(s)	892, 897
BstSFI	CTRYAG	2 cut(s)	501, 667
BstUI	CGCG	2 cut(s)	1113, 1115
BstV1I	GCAGC	3 cut(s)	283, 373, 646
BstV2I	GAAGAC	1 cut(s)	207
BstXI	CCANNNNNNTGG	1 cut(s)	893
Bsu15I	ATCGAT	1 cut(s)	744
BsuRI	GGCC	3 cut(s)	70, 499, 897
BsuTUI	ATCGAT	1 cut(s)	744
BtgZI	GCGATG	2 cut(s)	828, 1059
BtsIMutI	CAGTG	1 cut(s)	687
Cac8I	GCNNGC	2 cut(s)	501, 537
CaiI	CAGNNNCTG	1 cut(s)	104
CfoI	GCGC	1 cut(s)	1115
Cfr10I	RCCGGY	2 cut(s)	796, 1090
Cfr13I	GGNCC	2 cut(s)	617, 896
ClaI	ATCGAT	1 cut(s)	744
Csp6I	GTAC	1 cut(s)	764
CspAI	ACCGGT	1 cut(s)	1090
CviAII	CATG	5 cut(s)	193, 205, 281, 630, 829
CviQI	GTAC	1 cut(s)	764
DdeI	CTNAG	9 cut(s)	75, 236, 292, 678, 767, 843, 854, 922, 986
DpnI	GATC	5 cut(s)	234, 279, 395, 938, 1019
DpnII	GATC	5 cut(s)	232, 277, 393, 936, 1017
DraIII	CACNNNGTG	1 cut(s)	340
EaeI	YGGCCR	1 cut(s)	68
Eco24I	GRGCYC	1 cut(s)	961
Eco32I	GATATC	1 cut(s)	90
Eco47I	GGWCC	1 cut(s)	617
Eco57I	CTGAAG	3 cut(s)	219, 288, 704
EcoRII	CCWGG	1 cut(s)	892
EcoRV	GATATC	1 cut(s)	90
EcoT38I	GRGCYC	1 cut(s)	961
FaeI	CATG	5 cut(s)	196, 208, 284, 633, 832
FalI	AAGNNNNNCTT	4 cut(s)	398, 430, 933, 965
FatI	CATG	5 cut(s)	192, 204, 280, 629, 828
FbaI	TGATCA	2 cut(s)	277, 936
Fnu4HI	GCNGC	4 cut(s)	297, 387, 660, 1116
FriOI	GRGCYC	1 cut(s)	961
Fsp4HI	GCNGC	4 cut(s)	297, 387, 660, 1116
FspBI	CTAG	3 cut(s)	50, 390, 1100
GlaI	GCGC	1 cut(s)	1114
GluI	GCNGC	4 cut(s)	297, 387, 660, 1116
GsuI	CTGGAG	2 cut(s)	126, 639
HaeIII	GGCC	3 cut(s)	70, 499, 897
HapII	CCGG	3 cut(s)	797, 899, 1091
HhaI	GCGC	1 cut(s)	1115
Hin1II	CATG	5 cut(s)	196, 208, 284, 633, 832
Hin6I	GCGC	1 cut(s)	1113
HinP1I	GCGC	1 cut(s)	1113
HincII	GTYRAC	1 cut(s)	774
HindII	GTYRAC	1 cut(s)	774
HindIII	AAGCTT	3 cut(s)	533, 872, 947
HinfI	GANTC	2 cut(s)	79, 485
HpaII	CCGG	3 cut(s)	797, 899, 1091
HphI	GGTGA	3 cut(s)	811, 835, 875
Hpy166II	GTNNAC	3 cut(s)	764, 774, 802
Hpy188I	TCNGA	9 cut(s)	76, 172, 490, 681, 723, 784, 809, 925, 936
Hpy188III	TCNNGA	6 cut(s)	85, 325, 443, 512, 1003, 1021
Hpy8I	GTNNAC	3 cut(s)	764, 774, 802
Hpy99I	CGWCG	1 cut(s)	138
HpyCH4IV	ACGT	1 cut(s)	159
HpyCH4V	TGCA	2 cut(s)	119, 503
HpyF10VI	GCNNNNNNNGC	6 cut(s)	67, 428, 500, 505, 659, 1110
HpyF3I	CTNAG	9 cut(s)	75, 236, 292, 678, 767, 843, 854, 922, 986
HpySE526I	ACGT	1 cut(s)	159
Hsp92II	CATG	5 cut(s)	196, 208, 284, 633, 832
HspAI	GCGC	1 cut(s)	1113
Ksp22I	TGATCA	2 cut(s)	277, 936
Kzo9I	GATC	5 cut(s)	232, 277, 393, 936, 1017
LmnI	GCTCC	5 cut(s)	29, 46, 101, 658, 964
Lsp1109I	GCAGC	3 cut(s)	283, 373, 646
LweI	GCATC	3 cut(s)	128, 481, 505
MaeI	CTAG	3 cut(s)	50, 390, 1100
MaeII	ACGT	1 cut(s)	159
MaeIII	GTNAC	2 cut(s)	262, 917
MalI	GATC	5 cut(s)	234, 279, 395, 938, 1019
MboI	GATC	5 cut(s)	232, 277, 393, 936, 1017
MboII	GAAGA	1 cut(s)	212
MhlI	GDGCHC	1 cut(s)	961
MlsI	TGGCCA	1 cut(s)	70
MluCI	AATT	3 cut(s)	250, 591, 879
MluNI	TGGCCA	1 cut(s)	70
MlyI	GAGTC	1 cut(s)	494
MmeI	TCCRAC	2 cut(s)	438, 1057
MnlI	CCTC	7 cut(s)	101, 376, 402, 519, 538, 856, 973
Mox20I	TGGCCA	1 cut(s)	70
MroXI	GAANNNNTTC	1 cut(s)	906
MscI	TGGCCA	1 cut(s)	70
MseI	TTAA	3 cut(s)	288, 405, 450
Msp20I	TGGCCA	1 cut(s)	70
MspA1I	CMGCKG	1 cut(s)	659
MspI	CCGG	3 cut(s)	797, 899, 1091
MspR9I	CCNGG	2 cut(s)	894, 899
MvaI	CCWGG	1 cut(s)	894
MvnI	CGCG	2 cut(s)	1113, 1115
MwoI	GCNNNNNNNGC	6 cut(s)	67, 428, 500, 505, 659, 1110
NciI	CCSGG	1 cut(s)	899
NdeII	GATC	5 cut(s)	232, 277, 393, 936, 1017
NlaIII	CATG	5 cut(s)	196, 208, 284, 633, 832
NlaIV	GGNNCC	3 cut(s)	602, 654, 960
NspI	RCATGY	1 cut(s)	208
PciI	ACATGT	1 cut(s)	204
PdmI	GAANNNNTTC	1 cut(s)	906
PfeI	GAWTC	1 cut(s)	79
PinAI	ACCGGT	1 cut(s)	1090
PkrI	GCNGC	4 cut(s)	298, 388, 661, 1117
PleI	GAGTC	1 cut(s)	493
PpsI	GAGTC	1 cut(s)	493
Ppu21I	YACGTR	1 cut(s)	160
PscI	ACATGT	1 cut(s)	204
Psp6I	CCWGG	1 cut(s)	892
PspGI	CCWGG	1 cut(s)	892
PspN4I	GGNNCC	3 cut(s)	602, 654, 960
PspPI	GGNCC	2 cut(s)	617, 896
PstI	CTGCAG	1 cut(s)	505
PstNI	CAGNNNCTG	1 cut(s)	104
PvuII	CAGCTG	1 cut(s)	659
RsaI	GTAC	1 cut(s)	765
RsaNI	GTAC	1 cut(s)	764
SaqAI	TTAA	3 cut(s)	288, 405, 450
SatI	GCNGC	4 cut(s)	297, 387, 660, 1116
Sau3AI	GATC	5 cut(s)	232, 277, 393, 936, 1017
Sau96I	GGNCC	2 cut(s)	617, 896
SchI	GAGTC	1 cut(s)	494
ScrFI	CCNGG	2 cut(s)	894, 899
SduI	GDGCHC	1 cut(s)	961
SfaNI	GCATC	3 cut(s)	128, 481, 505
SfcI	CTRYAG	2 cut(s)	501, 667
SinI	GGWCC	1 cut(s)	617
SmlI	CTYRAG	3 cut(s)	83, 510, 1083
SmoI	CTYRAG	3 cut(s)	83, 510, 1083
Sse9I	AATT	3 cut(s)	250, 591, 879
SsiI	CCGC	2 cut(s)	1111, 1115
SspI	AATATT	1 cut(s)	65
SspMI	CTAG	3 cut(s)	50, 390, 1100
StyD4I	CCNGG	2 cut(s)	892, 897
TaiI	ACGT	1 cut(s)	162
TaqI	TCGA	4 cut(s)	425, 589, 744, 1020
TasI	AATT	3 cut(s)	250, 591, 879
TatI	WGTACW	1 cut(s)	763
TauI	GCSGC	1 cut(s)	1118
TfiI	GAWTC	1 cut(s)	79
Tru1I	TTAA	3 cut(s)	288, 405, 450
Tru9I	TTAA	3 cut(s)	288, 405, 450
TscAI	CASTG	1 cut(s)	694
TseI	GCWGC	3 cut(s)	296, 386, 659
TspDTI	ATGAA	3 cut(s)	103, 201, 845
TspGWI	ACGGA	2 cut(s)	557, 779
TspRI	CASTG	1 cut(s)	694
VpaK11BI	GGWCC	1 cut(s)	617
XceI	RCATGY	1 cut(s)	208
XcmI	CCANNNNNNNNNTGG	1 cut(s)	611
XmnI	GAANNNNTTC	1 cut(s)	906
XspI	CTAG	3 cut(s)	50, 390, 1100

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.