Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003420

Physical Location & Seq

Genomic Coordinates Reverse (-)

30414815 .. 30418128

Gene Structure 3314 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1224100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1380 bp

ATGGCTCTCTCTCTCTCTCTGAATTATTTATGGTCAAACCGTAAATTAGTAACGAGTGGCGTCCTATTGTTTGCTGTGGGACTGTGTCCCAATTATGAATATATACCAGCAGAACTTCACAGGCTGAGGGCCAAATACCCATATCATATCCCCTTTCATCTCCTCCGAACCCAACATTTTGCCTCTCATTCTTATTCCCAACTTCTCAAAGCTTCAAAGCTTCAAACTTTGCTTTGTCCGGCAGTTCTAATGGGAACTGCTGGTGATACTAAGTATGGTGCGTACACCTACGAGAACCTCGAGAGGGAGCCTTATTGGCCTTCTGAAAAGCTCCGAATTTCCATCACTGGGGCAGGTGGCTTTGTCGCCTCCCACATTGCCCGGAGATTGAAGAATGAGGGTCATTACATCATTGCTTCTGACTGGAAGAAGAATGACTACATGACTGAAGACATCTTCTGCCATGAATTCCATCTTGCTGACCTCAGGGTTATGGATAATTGCTTGAAGGTTACTAAGAATGTTGACCATGTGTTCAACCTCGCAGCTGATATGGGCGGAATGGGCTTCATTCAGTCCAACCATTCCGTCATCTTTTATAATAATACCATGATTTGCTTCAACATGGTTGAGGCTTCTCGGATCAATGATGTGAAGAGGTTTTTCTATGCTTCAAGTGCTTGTATTTATCCTGAATTTAAGCAGCTGGAAACCAATGTGAGCCTGAAGGAGTCTGATGCTTGGCCTGCGGAGCCTCAAGATGCTTATGGCTTGCAGAAGCTTGCAACTGAGGAATTGTGCAAGCACTACACAAAAGACTTTGGAATTGAGTGCCGTATTGGAAGGTTCCACAACACTTATGGTCCATTTGGAACCTGGAAAGGTGGAAGGGAGAAGGCTCCTGCTGCTTTTTGCAGAAAGGCACTCACTGCCACTGAAAAGTTTGAGATGTGGGGAGATGGACTTCAGACCCGATCCTTTACTTTTATTGATGAATGTGTAGAAGGTGTTCTTCGGTTGACGAAGTCGGACTTCCGCGAGCCACTGAATATTGGAAGTGATGAGATGGTTAGCATGAATGAGATGGCTGAGATGGTTCTTAGCTTTGAGGACAAGAAGCTGCCTATCCAGCATATTCCGGGGCCAGAGGGTGTCCGTGGTCGTAACTCGGACAACACACTGATCAAAGAGAAACTTGGTTGGGCTCCTACCATAAGGTTGAAGGGTGGTCTGAGAATTACATACTTCTGGATCAAGGAACAGATTGAGAAAGAGAAGGCACAAGGTGCTGACCTCTCGGTGTATGGCTCATCTAAGGTTGTCGGAACCCAAGCCCCAGTTCAACTTGGTTCGCTGCTTGCTGCTGATGGTAAAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00053 ko00520 ko01100 ko01110 map00053 map00520 map01100 map01110

Pfam Domains

Protein Families

Epimerase

Protein Analysis

460

Amino Acids

51.98

Weight (kDa)

7.59

Isoelectric Point (pI)

39.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	113 - 352	1.2e-43	NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd	PF16363	115 - 412	8.1e-25	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	115 - 267	2.1e-07	3-beta hydroxysteroid dehydrogenase/isomerase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003420)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G28840 AT5G28840
fragaria_vesca	FvH4_6g02980
malus_domestica	MD04G1224100.v1.1 MD04G1224200.v1.1 MD12G1240900.v1.1
prunus_persica	Prupe.1G025100_v2.0.a1 Prupe.1G025100_v2.0.a1 Prupe.6G342800_v2.0.a1
pyrus_communis	pycom04g19750
rosa_chinensis	RchiOBHm_Chr3g0451161 RchiOBHm_Chr4g0388911
rosa_laevigata	RLG00000010008 RLG00000010011 RLG00000025681 RLG00000025682
rosa_multiflora	Rmu_co8299443.1_g000001 Rmu_sc0005486.1_g000010 Rmu_ssc0000028.1_g000003
rosa_roxburghii	Rroxscaffold_5G00335220 Rroxscaffold_6G00428630
rosa_rugosa	Rorug02G0632400 Rorug02G0632500 Rorug03G0321000 Rorug03G0321000 Rorug03G0321100
rosa_samantha	Rh3AG034900 Rh3BG035300 Rh3CG033800 Rh3CG033900 Rh3DG034900 Rh4BG018800 Rh4CG028600 Rh4DG019300
rosa_wichuraiana	Rw3G002560 Rw4G001840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	600
AarI	CACCTGC	1 cut(s)	344
Acc36I	ACCTGC	1 cut(s)	344
AccII	CGCG	1 cut(s)	1038
AciI	CCGC	3 cut(s)	558, 749, 1036
AclWI	GGATC	3 cut(s)	650, 969, 1259
AcsI	RAATTY	3 cut(s)	336, 467, 695
AcuI	CTGAAG	3 cut(s)	468, 746, 950
AcyI	GRCGYC	1 cut(s)	60
AdeI	CACNNNGTG	1 cut(s)	1286
AfaI	GTAC	1 cut(s)	284
AfiI	CCNNNNNNNGG	2 cut(s)	304, 348
AgsI	TTSAA	9 cut(s)	216, 224, 391, 508, 538, 622, 675, 1222, 1343
AjnI	CCWGG	1 cut(s)	875
AjuI	GAANNNNNNNTTGG	2 cut(s)	1323, 1355
AluBI	AGCT	8 cut(s)	212, 220, 331, 548, 706, 781, 1104, 1120
AluI	AGCT	8 cut(s)	212, 220, 331, 548, 706, 781, 1104, 1120
AlwI	GGATC	3 cut(s)	650, 969, 1259
Ama87I	CYCGRG	1 cut(s)	299
AoxI	GGCC	4 cut(s)	129, 317, 743, 1142
ApeKI	GCWGC	6 cut(s)	545, 703, 905, 1120, 1354, 1361
ApoI	RAATTY	3 cut(s)	336, 467, 695
Asp700I	GAANNNNTTC	1 cut(s)	1008
AspS9I	GGNCC	3 cut(s)	129, 863, 1142
AsuC2I	CCSGG	2 cut(s)	382, 1140
AsuHPI	GGTGA	1 cut(s)	275
AvaI	CYCGRG	1 cut(s)	299
AvaII	GGWCC	1 cut(s)	863
AxyI	CCTNAGG	1 cut(s)	485
BanII	GRGCYC	1 cut(s)	1207
BarI	GAAGNNNNNNTAC	2 cut(s)	422, 454
BbsI	GAAGAC	1 cut(s)	456
BbvCI	CCTCAGC	1 cut(s)	125
BbvI	GCAGC	6 cut(s)	557, 715, 892, 1107, 1341, 1348
BccI	CCATC	7 cut(s)	350, 480, 953, 1060, 1078, 1087, 1361
BceAI	ACGGC	1 cut(s)	819
BciT130I	CCWGG	1 cut(s)	877
BclI	TGATCA	1 cut(s)	1182
BcnI	CCSGG	2 cut(s)	382, 1140
BfuAI	ACCTGC	1 cut(s)	344
BglI	GCCNNNNNGGC	1 cut(s)	316
BisI	GCNGC	6 cut(s)	546, 704, 906, 1121, 1355, 1362
BlsI	GCNGC	6 cut(s)	547, 705, 907, 1122, 1356, 1363
Bme1390I	CCNGG	3 cut(s)	382, 877, 1140
Bme18I	GGWCC	1 cut(s)	863
BmeT110I	CYCGRG	1 cut(s)	299
BmgT120I	GGNCC	3 cut(s)	129, 863, 1142
BmiI	GGNNCC	8 cut(s)	309, 753, 848, 874, 900, 1143, 1206, 1327
BmrFI	CCNGG	3 cut(s)	382, 877, 1140
BmrI	ACTGGG	2 cut(s)	357, 1331
BmsI	GCATC	2 cut(s)	727, 751
BmuI	ACTGGG	2 cut(s)	357, 1331
BpiI	GAAGAC	1 cut(s)	456
Bpu10I	CCTNAGC	1 cut(s)	125
BpuEI	CTTGAG	1 cut(s)	741
BpuMI	CCSGG	2 cut(s)	382, 1140
BsaHI	GRCGYC	1 cut(s)	60
BsaJI	CCNNGG	2 cut(s)	1139, 1156
Bsc4I	CCNNNNNNNGG	2 cut(s)	304, 348
Bse1I	ACTGG	3 cut(s)	352, 428, 1337
Bse21I	CCTNAGG	1 cut(s)	485
Bse3DI	GCAATG	2 cut(s)	375, 411
BseBI	CCWGG	1 cut(s)	877
BseDI	CCNNGG	2 cut(s)	1139, 1156
BseLI	CCNNNNNNNGG	2 cut(s)	304, 348
BseMI	GCAATG	2 cut(s)	375, 411
BseMII	CTCAG	5 cut(s)	116, 499, 780, 1080, 1223
BseNI	ACTGG	3 cut(s)	352, 428, 1337
BseRI	GAGGAG	1 cut(s)	152
BseXI	GCAGC	6 cut(s)	557, 715, 892, 1107, 1341, 1348
Bsh1236I	CGCG	1 cut(s)	1038
BshFI	GGCC	4 cut(s)	131, 319, 745, 1144
BsiHKCI	CYCGRG	1 cut(s)	299
BsiSI	CCGG	3 cut(s)	239, 382, 1139
BslFI	GGGAC	2 cut(s)	72, 93
BslI	CCNNNNNNNGG	2 cut(s)	304, 348
BsmFI	GGGAC	2 cut(s)	72, 93
BsnI	GGCC	4 cut(s)	131, 319, 745, 1144
BsoBI	CYCGRG	1 cut(s)	299
Bsp1286I	GDGCHC	1 cut(s)	1207
Bsp143I	GATC	4 cut(s)	642, 974, 1182, 1251
BspACI	CCGC	3 cut(s)	558, 749, 1036
BspANI	GGCC	4 cut(s)	131, 319, 745, 1144
BspCNI	CTCAG	5 cut(s)	117, 498, 781, 1081, 1224
BspFNI	CGCG	1 cut(s)	1038
BspLI	GGNNCC	8 cut(s)	309, 753, 848, 874, 900, 1143, 1206, 1327
BspMI	ACCTGC	1 cut(s)	344
BspPI	GGATC	3 cut(s)	650, 969, 1259
BsrDI	GCAATG	2 cut(s)	375, 411
BsrI	ACTGG	3 cut(s)	352, 428, 1337
BssECI	CCNNGG	2 cut(s)	1139, 1156
BssMI	GATC	4 cut(s)	642, 974, 1182, 1251
BssNI	GRCGYC	1 cut(s)	60
Bst2UI	CCWGG	1 cut(s)	877
Bst4CI	ACNGT	2 cut(s)	41, 84
Bst6I	CTCTTC	1 cut(s)	650
BstACI	GRCGYC	1 cut(s)	60
BstAPI	GCANNNNNTGC	2 cut(s)	929, 1286
BstC8I	GCNNGC	6 cut(s)	747, 773, 783, 803, 1040, 1359
BstDEI	CTNAG	9 cut(s)	125, 270, 485, 516, 789, 1089, 1100, 1232, 1314
BstDSI	CCRYGG	1 cut(s)	1156
BstFNI	CGCG	1 cut(s)	1038
BstKTI	GATC	4 cut(s)	645, 977, 1185, 1254
BstMBI	GATC	4 cut(s)	642, 974, 1182, 1251
BstMWI	GCNNNNNNNGC	9 cut(s)	316, 564, 677, 746, 751, 905, 929, 1129, 1286
BstNI	CCWGG	1 cut(s)	877
BstSCI	CCNGG	3 cut(s)	380, 875, 1138
BstUI	CGCG	1 cut(s)	1038
BstV1I	GCAGC	6 cut(s)	557, 715, 892, 1107, 1341, 1348
BstV2I	GAAGAC	1 cut(s)	456
Bsu36I	CCTNAGG	1 cut(s)	485
BsuRI	GGCC	4 cut(s)	131, 319, 745, 1144
BtgI	CCRYGG	1 cut(s)	1156
BtsI	GCAGTG	1 cut(s)	927
BtsIMutI	CAGTG	5 cut(s)	345, 927, 933, 1043, 1178
BveI	ACCTGC	1 cut(s)	344
Cac8I	GCNNGC	6 cut(s)	747, 773, 783, 803, 1040, 1359
Cfr13I	GGNCC	3 cut(s)	129, 863, 1142
CseI	GACGC	1 cut(s)	49
Csp6I	GTAC	1 cut(s)	283
CviAII	CATG	6 cut(s)	442, 464, 530, 610, 625, 1075
CviQI	GTAC	1 cut(s)	283
DdeI	CTNAG	9 cut(s)	125, 270, 485, 516, 789, 1089, 1100, 1232, 1314
DpnI	GATC	4 cut(s)	644, 976, 1184, 1253
DpnII	GATC	4 cut(s)	642, 974, 1182, 1251
DraIII	CACNNNGTG	1 cut(s)	1286
Eam1104I	CTCTTC	1 cut(s)	650
EarI	CTCTTC	1 cut(s)	650
EciI	GGCGGA	1 cut(s)	573
Eco24I	GRGCYC	1 cut(s)	1207
Eco47I	GGWCC	1 cut(s)	863
Eco57I	CTGAAG	3 cut(s)	468, 746, 950
Eco81I	CCTNAGG	1 cut(s)	485
Eco88I	CYCGRG	1 cut(s)	299
EcoRI	GAATTC	1 cut(s)	467
EcoRII	CCWGG	1 cut(s)	875
EcoT38I	GRGCYC	1 cut(s)	1207
FaeI	CATG	6 cut(s)	445, 467, 533, 613, 628, 1078
FalI	AAGNNNNNCTT	6 cut(s)	996, 1028, 1016, 1048, 1179, 1211
FaqI	GGGAC	2 cut(s)	72, 93
FatI	CATG	6 cut(s)	441, 463, 529, 609, 624, 1074
FbaI	TGATCA	1 cut(s)	1182
Fnu4HI	GCNGC	6 cut(s)	546, 704, 906, 1121, 1355, 1362
FriOI	GRGCYC	1 cut(s)	1207
Fsp4HI	GCNGC	6 cut(s)	546, 704, 906, 1121, 1355, 1362
GluI	GCNGC	6 cut(s)	546, 704, 906, 1121, 1355, 1362
HaeIII	GGCC	4 cut(s)	131, 319, 745, 1144
HapII	CCGG	3 cut(s)	239, 382, 1139
HgaI	GACGC	1 cut(s)	49
Hin1I	GRCGYC	1 cut(s)	60
Hin1II	CATG	6 cut(s)	445, 467, 533, 613, 628, 1078
HincII	GTYRAC	2 cut(s)	526, 1020
HindII	GTYRAC	2 cut(s)	526, 1020
HindIII	AAGCTT	3 cut(s)	210, 218, 779
HinfI	GANTC	1 cut(s)	731
HpaII	CCGG	3 cut(s)	239, 382, 1139
HphI	GGTGA	1 cut(s)	275
Hpy166II	GTNNAC	3 cut(s)	285, 526, 1020
Hpy188III	TCNNGA	4 cut(s)	301, 692, 758, 1249
Hpy8I	GTNNAC	3 cut(s)	285, 526, 1020
HpyAV	CCTTC	9 cut(s)	330, 502, 721, 837, 882, 889, 998, 1216, 1270
HpyCH4III	ACNGT	2 cut(s)	41, 84
HpyCH4V	TGCA	4 cut(s)	775, 785, 801, 915
HpyF10VI	GCNNNNNNNGC	9 cut(s)	316, 564, 677, 746, 751, 905, 929, 1129, 1286
HpyF3I	CTNAG	9 cut(s)	125, 270, 485, 516, 789, 1089, 1100, 1232, 1314
Hsp92I	GRCGYC	1 cut(s)	60
Hsp92II	CATG	6 cut(s)	445, 467, 533, 613, 628, 1078
Ksp22I	TGATCA	1 cut(s)	1182
Kzo9I	GATC	4 cut(s)	642, 974, 1182, 1251
LmnI	GCTCC	5 cut(s)	307, 336, 751, 904, 1210
Lsp1109I	GCAGC	6 cut(s)	557, 715, 892, 1107, 1341, 1348
LweI	GCATC	2 cut(s)	727, 751
MaeIII	GTNAC	3 cut(s)	49, 511, 1163
MalI	GATC	4 cut(s)	644, 976, 1184, 1253
MboI	GATC	4 cut(s)	642, 974, 1182, 1251
MboII	GAAGA	7 cut(s)	403, 439, 442, 448, 461, 667, 1004
MhlI	GDGCHC	1 cut(s)	1207
MlyI	GAGTC	1 cut(s)	740
MmeI	TCCRAC	3 cut(s)	603, 1008, 1303
MroXI	GAANNNNTTC	1 cut(s)	1008
MseI	TTAA	1 cut(s)	699
MspA1I	CMGCKG	2 cut(s)	548, 706
MspI	CCGG	3 cut(s)	239, 382, 1139
MspR9I	CCNGG	3 cut(s)	382, 877, 1140
MvaI	CCWGG	1 cut(s)	877
MvnI	CGCG	1 cut(s)	1038
MwoI	GCNNNNNNNGC	9 cut(s)	316, 564, 677, 746, 751, 905, 929, 1129, 1286
NciI	CCSGG	2 cut(s)	382, 1140
NdeII	GATC	4 cut(s)	642, 974, 1182, 1251
NlaIII	CATG	6 cut(s)	445, 467, 533, 613, 628, 1078
NlaIV	GGNNCC	8 cut(s)	309, 753, 848, 874, 900, 1143, 1206, 1327
PaeR7I	CTCGAG	1 cut(s)	299
PaqCI	CACCTGC	1 cut(s)	344
PcsI	WCGNNNNNNNCGW	1 cut(s)	297
PdmI	GAANNNNTTC	1 cut(s)	1008
PflFI	GACNNNGTC	2 cut(s)	84, 1024
PkrI	GCNGC	6 cut(s)	547, 705, 907, 1122, 1356, 1363
PleI	GAGTC	1 cut(s)	739
PpsI	GAGTC	1 cut(s)	739
PsiI	TTATAA	1 cut(s)	600
Psp6I	CCWGG	1 cut(s)	875
PspGI	CCWGG	1 cut(s)	875
PspN4I	GGNNCC	8 cut(s)	309, 753, 848, 874, 900, 1143, 1206, 1327
PspPI	GGNCC	3 cut(s)	129, 863, 1142
PsrI	GAACNNNNNNTAC	2 cut(s)	993, 1025
PsyI	GACNNNGTC	2 cut(s)	84, 1024
PvuII	CAGCTG	2 cut(s)	548, 706
RsaI	GTAC	1 cut(s)	284
RsaNI	GTAC	1 cut(s)	283
SaqAI	TTAA	1 cut(s)	699
SatI	GCNGC	6 cut(s)	546, 704, 906, 1121, 1355, 1362
Sau3AI	GATC	4 cut(s)	642, 974, 1182, 1251
Sau96I	GGNCC	3 cut(s)	129, 863, 1142
SchI	GAGTC	1 cut(s)	740
ScrFI	CCNGG	3 cut(s)	382, 877, 1140
SduI	GDGCHC	1 cut(s)	1207
SfaNI	GCATC	2 cut(s)	727, 751
Sfr274I	CTCGAG	1 cut(s)	299
SinI	GGWCC	1 cut(s)	863
SlaI	CTCGAG	1 cut(s)	299
SmlI	CTYRAG	2 cut(s)	299, 756
SmoI	CTYRAG	2 cut(s)	299, 756
SsiI	CCGC	3 cut(s)	558, 749, 1036
SspI	AATATT	1 cut(s)	1051
StyD4I	CCNGG	3 cut(s)	380, 875, 1138
TaaI	ACNGT	2 cut(s)	41, 84
TaqI	TCGA	1 cut(s)	300
Tru1I	TTAA	1 cut(s)	699
Tru9I	TTAA	1 cut(s)	699
TscAI	CASTG	5 cut(s)	352, 934, 940, 1050, 1185
TseI	GCWGC	6 cut(s)	545, 703, 905, 1120, 1354, 1361
TspDTI	ATGAA	6 cut(s)	111, 146, 480, 559, 1008, 1091
TspGWI	ACGGA	2 cut(s)	577, 1145
TspRI	CASTG	5 cut(s)	352, 934, 940, 1050, 1185
Tth111I	GACNNNGTC	2 cut(s)	84, 1024
VpaK11BI	GGWCC	1 cut(s)	863
XapI	RAATTY	3 cut(s)	336, 467, 695
XcmI	CCANNNNNNNNNTGG	2 cut(s)	857, 873
XhoI	CTCGAG	1 cut(s)	299
XmnI	GAANNNNTTC	1 cut(s)	1008

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.