Rh3BG035300

GDP-mannose 3,5-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0003420
Physical Location & Seq
Forward (+)
2328316 .. 2332141
3826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG035300.1

Sequence Viewer

Length: 1149 bp
ATGAATCTGTCTTGTAGTATGGGAAGTGCTGGTGAAAGTGGTTATGGTGCATACACATATGAGGCCCTTGAGAGGGAGCCTTATTGGCCCTCAGAAAAACTCCGAATTTCCATTACTGGGGCTGGTGGCTTTATTGCCTCCCACATTGCCCGCCGATTGAAGAATGAGGGCCACTACATTATTGCCTCTGACTGGAAGAAGAATGAGCACATGAGTGAAGACATGTTCTGCCATGAATTCCATCTTGCGGACCTCAGGGTCATGGATAACTGCTTGAAGGTTACCAAGGATGTCGATCATGTGTTCAACCTTGCTGCTGATATGGGCGGCATGGGATTCATTCAGTCCAACCACTCTGTCATTATGTACAATAATACCATGATCAGCTTTAACATGCTGGAAGCTGCTAGGATCAGTGGAGTGAAAAGGTTATTCTATGCTTCCAGTGCTTGTATTTATCCCGAGTTTAAGCAGCTGGAAACCAATGTGAGCTTGAAGGAGGCTGATGCCTGGCCTGCAGAGCCTCAAGATGCTTATGGCTTAGAGAAACTTGCAACTGAGGAGTTGTGCAAGCACTACACCAAAGACTTTGGAATCGAATGCCGTATTGGAAGGTTCCACAACATCTATGGTCCTTTCGGAACCTGGAAAGGGGGCAGGGAGAAGGCTCCTGCTGCTTTCTGCAGAAAGGCTCTCACTTCCACTGATAAGTTTGAGATGTGGGGAGATGGACTCCAGACCCGATCCTTCACCTTTATTGATGAATGTGTTGAAGGTGTACTCCGGTTGACAAAGTCCGACTTCCGTGAGCCAGTGAATATTGGAAGCGATGAGATGGTTAGCATGAATGAGATGGCTGAGATTGTTCTTAGCTTTGAGAACAAGAAGCTGCCTATTCAACACATTCCTGGGCCTGAGGGTGTCCGTGGTCGTAACTCTGACAACACCCTGATCAAAGAGAAACTTGGCTGGGCTCCTACCATGAGGCTGAAGGATGGACTGAGGTTTACATACTTCTGGATCAAGGAACAGATTGAGAAAGAGAAAGCACAAGGTACTGACCTGTCGCTTTATGGGTCATCTAAGGTGGTGGGAACTCAAGCCCCAGTTCAACTTGGTTCACTTCGTGCCGCTGATGGCAAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko00520 ko01100 ko01110 map00053 map00520 map01100 map01110
Pfam Domains
Protein Families
Epimerase

Protein Analysis

382

Amino Acids

42.99

Weight (kDa)

5.99

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 36 - 275 2.2e-45 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 38 - 335 1.1e-26 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 38 - 190 1.6e-09 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 257
AciI CCGC 4 cut(s) 151, 248, 327, 1131
AclWI GGATC 3 cut(s) 419, 738, 1028
AcsI RAATTY 2 cut(s) 105, 236
AcuI CTGAAG 1 cut(s) 1010
AdeI CACNNNGTG 1 cut(s) 1127
AfaI GTAC 3 cut(s) 368, 780, 1057
AfiI CCNNNNNNNGG 6 cut(s) 72, 73, 117, 192, 247, 651
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 7 cut(s) 160, 277, 307, 496, 773, 899, 1112
AjnI CCWGG 3 cut(s) 509, 644, 907
AjuI GAANNNNNNNTTGG 2 cut(s) 591, 623
AleI CACNNNNGTG 1 cut(s) 213
AluBI AGCT 6 cut(s) 387, 404, 475, 492, 873, 889
AluI AGCT 6 cut(s) 387, 404, 475, 492, 873, 889
Alw21I GWGCWC 1 cut(s) 210
AlwI GGATC 3 cut(s) 419, 738, 1028
Ama87I CYCGRG 1 cut(s) 461
AoxI GGCC 5 cut(s) 63, 86, 169, 512, 911
ApeKI GCWGC 5 cut(s) 314, 404, 472, 674, 889
ApoI RAATTY 2 cut(s) 105, 236
AspS9I GGNCC 6 cut(s) 64, 87, 169, 250, 632, 911
AsuHPI GGTGA 2 cut(s) 44, 742
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 2 cut(s) 250, 632
AxyI CCTNAGG 2 cut(s) 254, 915
BanII GRGCYC 1 cut(s) 976
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 210
BbvI GCAGC 5 cut(s) 301, 391, 484, 661, 876
BccI CCATC 6 cut(s) 249, 722, 829, 847, 989, 1130
BceAI ACGGC 1 cut(s) 588
BciT130I CCWGG 3 cut(s) 511, 646, 909
BclI TGATCA 2 cut(s) 381, 951
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 2 cut(s) 516, 682
BglI GCCNNNNNGGC 1 cut(s) 85
BisI GCNGC 7 cut(s) 315, 328, 405, 473, 675, 890, 1131
BlsI GCNGC 7 cut(s) 316, 329, 406, 474, 676, 891, 1132
Bme1390I CCNGG 3 cut(s) 511, 646, 909
Bme18I GGWCC 2 cut(s) 250, 632
BmeT110I CYCGRG 1 cut(s) 461
BmgT120I GGNCC 6 cut(s) 64, 87, 169, 250, 632, 911
BmiI GGNNCC 5 cut(s) 78, 617, 643, 669, 975
BmrFI CCNGG 3 cut(s) 511, 646, 909
BmrI ACTGGG 2 cut(s) 126, 1100
BmsI GCATC 2 cut(s) 496, 520
BmuI ACTGGG 2 cut(s) 126, 1100
BpiI GAAGAC 1 cut(s) 225
BplI GAGNNNNNCTC 2 cut(s) 717, 749
BpmI CTGGAG 1 cut(s) 719
BpuEI CTTGAG 3 cut(s) 89, 510, 1083
BsaBI GATNNNNATC 1 cut(s) 294
BsaJI CCNNGG 3 cut(s) 285, 908, 925
BsaWI WCCGGW 1 cut(s) 783
Bsc4I CCNNNNNNNGG 6 cut(s) 72, 73, 117, 192, 247, 651
Bse1I ACTGG 5 cut(s) 121, 197, 444, 812, 1106
Bse21I CCTNAGG 2 cut(s) 254, 915
Bse3DI GCAATG 1 cut(s) 144
Bse8I GATNNNNATC 1 cut(s) 294
BseBI CCWGG 3 cut(s) 511, 646, 909
BseDI CCNNGG 3 cut(s) 285, 908, 925
BseGI GGATG 2 cut(s) 295, 1000
BseJI GATNNNNATC 1 cut(s) 294
BseLI CCNNNNNNNGG 6 cut(s) 72, 73, 117, 192, 247, 651
BseMI GCAATG 1 cut(s) 144
BseMII CTCAG 6 cut(s) 105, 268, 549, 849, 906, 992
BseNI ACTGG 5 cut(s) 121, 197, 444, 812, 1106
BseRI GAGGAG 1 cut(s) 575
BseXI GCAGC 5 cut(s) 301, 391, 484, 661, 876
BseYI CCCAGC 1 cut(s) 969
BshFI GGCC 5 cut(s) 65, 88, 171, 514, 913
BsiHKAI GWGCWC 1 cut(s) 210
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 1 cut(s) 784
BslI CCNNNNNNNGG 6 cut(s) 72, 73, 117, 192, 247, 651
BsmI GAATGC 1 cut(s) 605
BsnI GGCC 5 cut(s) 65, 88, 171, 514, 913
BsoBI CYCGRG 1 cut(s) 461
Bsp1286I GDGCHC 2 cut(s) 210, 976
Bsp1407I TGTACA 1 cut(s) 366
Bsp143I GATC 6 cut(s) 295, 381, 411, 743, 951, 1020
BspACI CCGC 4 cut(s) 151, 248, 327, 1131
BspANI GGCC 5 cut(s) 65, 88, 171, 514, 913
BspCNI CTCAG 6 cut(s) 104, 267, 550, 850, 907, 993
BspLI GGNNCC 5 cut(s) 78, 617, 643, 669, 975
BspMAI CTGCAG 2 cut(s) 520, 686
BspPI GGATC 3 cut(s) 419, 738, 1028
BsrDI GCAATG 1 cut(s) 144
BsrGI TGTACA 1 cut(s) 366
BsrI ACTGG 5 cut(s) 121, 197, 444, 812, 1106
BssECI CCNNGG 3 cut(s) 285, 908, 925
BssMI GATC 6 cut(s) 295, 381, 411, 743, 951, 1020
BssT1I CCWWGG 1 cut(s) 285
Bst2UI CCWGG 3 cut(s) 511, 646, 909
BstAUI TGTACA 1 cut(s) 366
BstC8I GCNNGC 3 cut(s) 151, 516, 572
BstDEI CTNAG 9 cut(s) 91, 254, 541, 558, 858, 869, 915, 1001, 1083
BstDSI CCRYGG 1 cut(s) 925
BstEII GGTNACC 1 cut(s) 280
BstF5I GGATG 2 cut(s) 295, 1000
BstKTI GATC 6 cut(s) 298, 384, 414, 746, 954, 1023
BstMBI GATC 6 cut(s) 295, 381, 411, 743, 951, 1020
BstMWI GCNNNNNNNGC 5 cut(s) 85, 446, 515, 520, 674
BstNI CCWGG 3 cut(s) 511, 646, 909
BstNSI RCATGY 2 cut(s) 226, 397
BstPI GGTNACC 1 cut(s) 280
BstSCI CCNGG 3 cut(s) 509, 644, 907
BstSFI CTRYAG 2 cut(s) 516, 682
BstV1I GCAGC 5 cut(s) 301, 391, 484, 661, 876
BstV2I GAAGAC 1 cut(s) 225
Bsu36I CCTNAGG 2 cut(s) 254, 915
BsuRI GGCC 5 cut(s) 65, 88, 171, 514, 913
BtgI CCRYGG 1 cut(s) 925
BtgZI GCGATG 1 cut(s) 843
BtsCI GGATG 2 cut(s) 295, 1000
BtsIMutI CAGTG 4 cut(s) 421, 451, 702, 819
Cac8I GCNNGC 3 cut(s) 151, 516, 572
Cfr13I GGNCC 6 cut(s) 64, 87, 169, 250, 632, 911
Csp6I GTAC 3 cut(s) 367, 779, 1056
CviQI GTAC 3 cut(s) 367, 779, 1056
DdeI CTNAG 9 cut(s) 91, 254, 541, 558, 858, 869, 915, 1001, 1083
DpnI GATC 6 cut(s) 297, 383, 413, 745, 953, 1022
DpnII GATC 6 cut(s) 295, 381, 411, 743, 951, 1020
DraIII CACNNNGTG 1 cut(s) 1127
DrdI GACNNNNNNGTC 1 cut(s) 257
DseDI GACNNNNNNGTC 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 976
Eco47I GGWCC 2 cut(s) 250, 632
Eco57I CTGAAG 1 cut(s) 1010
Eco81I CCTNAGG 2 cut(s) 254, 915
Eco88I CYCGRG 1 cut(s) 461
Eco91I GGTNACC 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 64
EcoO65I GGTNACC 1 cut(s) 280
EcoRI GAATTC 1 cut(s) 236
EcoRII CCWGG 3 cut(s) 509, 644, 907
EcoT14I CCWWGG 1 cut(s) 285
EcoT38I GRGCYC 1 cut(s) 976
ErhI CCWWGG 1 cut(s) 285
FalI AAGNNNNNCTT 4 cut(s) 785, 817, 948, 980
FauI CCCGC 1 cut(s) 158
FauNDI CATATG 1 cut(s) 58
FbaI TGATCA 2 cut(s) 381, 951
Fnu4HI GCNGC 7 cut(s) 315, 328, 405, 473, 675, 890, 1131
FokI GGATG 2 cut(s) 302, 1007
FriOI GRGCYC 1 cut(s) 976
Fsp4HI GCNGC 7 cut(s) 315, 328, 405, 473, 675, 890, 1131
FspBI CTAG 1 cut(s) 408
GluI GCNGC 7 cut(s) 315, 328, 405, 473, 675, 890, 1131
GsaI CCCAGC 1 cut(s) 973
GsuI CTGGAG 1 cut(s) 719
HaeIII GGCC 5 cut(s) 65, 88, 171, 514, 913
HapII CCGG 1 cut(s) 784
HincII GTYRAC 1 cut(s) 789
HindII GTYRAC 1 cut(s) 789
HinfI GANTC 4 cut(s) 4, 336, 594, 732
HpaII CCGG 1 cut(s) 784
HphI GGTGA 2 cut(s) 44, 742
Hpy166II GTNNAC 4 cut(s) 779, 789, 1008, 1121
Hpy188I TCNGA 6 cut(s) 94, 104, 190, 641, 799, 940
Hpy188III TCNNGA 4 cut(s) 461, 527, 736, 1018
Hpy8I GTNNAC 4 cut(s) 779, 789, 1008, 1121
HpyAV CCTTC 7 cut(s) 271, 490, 606, 658, 757, 767, 985
HpyCH4V TGCA 5 cut(s) 50, 518, 554, 570, 684
HpyF10VI GCNNNNNNNGC 5 cut(s) 85, 446, 515, 520, 674
HpyF3I CTNAG 9 cut(s) 91, 254, 541, 558, 858, 869, 915, 1001, 1083
Ksp22I TGATCA 2 cut(s) 381, 951
Kzo9I GATC 6 cut(s) 295, 381, 411, 743, 951, 1020
LmnI GCTCC 3 cut(s) 76, 673, 979
Lsp1109I GCAGC 5 cut(s) 301, 391, 484, 661, 876
LweI GCATC 2 cut(s) 496, 520
MaeI CTAG 1 cut(s) 408
MaeIII GTNAC 2 cut(s) 280, 932
MalI GATC 6 cut(s) 297, 383, 413, 745, 953, 1022
MboI GATC 6 cut(s) 295, 381, 411, 743, 951, 1020
MboII GAAGA 4 cut(s) 172, 208, 211, 230
MhlI GDGCHC 2 cut(s) 210, 976
MluCI AATT 2 cut(s) 105, 236
MlyI GAGTC 1 cut(s) 726
MmeI TCCRAC 2 cut(s) 372, 822
MseI TTAA 2 cut(s) 390, 468
MslI CAYNNNNRTG 1 cut(s) 213
MspA1I CMGCKG 2 cut(s) 475, 1133
MspI CCGG 1 cut(s) 784
MspR9I CCNGG 3 cut(s) 511, 646, 909
Mva1269I GAATGC 1 cut(s) 605
MvaI CCWGG 3 cut(s) 511, 646, 909
MwoI GCNNNNNNNGC 5 cut(s) 85, 446, 515, 520, 674
NdeI CATATG 1 cut(s) 58
NdeII GATC 6 cut(s) 295, 381, 411, 743, 951, 1020
NlaIV GGNNCC 5 cut(s) 78, 617, 643, 669, 975
NspI RCATGY 2 cut(s) 226, 397
OliI CACNNNNGTG 1 cut(s) 213
PciI ACATGT 1 cut(s) 222
PctI GAATGC 1 cut(s) 605
PfeI GAWTC 3 cut(s) 4, 336, 594
PflFI GACNNNGTC 1 cut(s) 793
PkrI GCNGC 7 cut(s) 316, 329, 406, 474, 676, 891, 1132
PleI GAGTC 1 cut(s) 726
PpsI GAGTC 1 cut(s) 726
PscI ACATGT 1 cut(s) 222
Psp6I CCWGG 3 cut(s) 509, 644, 907
PspEI GGTNACC 1 cut(s) 280
PspFI CCCAGC 1 cut(s) 969
PspGI CCWGG 3 cut(s) 509, 644, 907
PspN4I GGNNCC 5 cut(s) 78, 617, 643, 669, 975
PspPI GGNCC 6 cut(s) 64, 87, 169, 250, 632, 911
PstI CTGCAG 2 cut(s) 520, 686
PsyI GACNNNGTC 1 cut(s) 793
PvuII CAGCTG 1 cut(s) 475
RsaI GTAC 3 cut(s) 368, 780, 1057
RsaNI GTAC 3 cut(s) 367, 779, 1056
RseI CAYNNNNRTG 1 cut(s) 213
SaqAI TTAA 2 cut(s) 390, 468
SatI GCNGC 7 cut(s) 315, 328, 405, 473, 675, 890, 1131
Sau3AI GATC 6 cut(s) 295, 381, 411, 743, 951, 1020
Sau96I GGNCC 6 cut(s) 64, 87, 169, 250, 632, 911
SchI GAGTC 1 cut(s) 726
ScrFI CCNGG 3 cut(s) 511, 646, 909
SduI GDGCHC 2 cut(s) 210, 976
SfaNI GCATC 2 cut(s) 496, 520
SfcI CTRYAG 2 cut(s) 516, 682
SinI GGWCC 2 cut(s) 250, 632
SmiMI CAYNNNNRTG 1 cut(s) 213
SmlI CTYRAG 3 cut(s) 68, 525, 1098
SmoI CTYRAG 3 cut(s) 68, 525, 1098
Sse9I AATT 2 cut(s) 105, 236
SsiI CCGC 4 cut(s) 151, 248, 327, 1131
SspI AATATT 1 cut(s) 820
SspMI CTAG 1 cut(s) 408
StyD4I CCNGG 3 cut(s) 509, 644, 907
StyI CCWWGG 1 cut(s) 285
TaqI TCGA 2 cut(s) 294, 597
TasI AATT 2 cut(s) 105, 236
TatI WGTACW 2 cut(s) 366, 778
TauI GCSGC 2 cut(s) 330, 1133
TfiI GAWTC 3 cut(s) 4, 336, 594
Tru1I TTAA 2 cut(s) 390, 468
Tru9I TTAA 2 cut(s) 390, 468
TscAI CASTG 4 cut(s) 421, 451, 709, 819
TseI GCWGC 5 cut(s) 314, 404, 472, 674, 889
TspDTI ATGAA 5 cut(s) 17, 249, 328, 777, 860
TspGWI ACGGA 2 cut(s) 794, 914
TspRI CASTG 4 cut(s) 421, 451, 709, 819
Tth111I GACNNNGTC 1 cut(s) 793
VpaK11BI GGWCC 2 cut(s) 250, 632
XapI RAATTY 2 cut(s) 105, 236
XceI RCATGY 2 cut(s) 226, 397
XcmI CCANNNNNNNNNTGG 1 cut(s) 626
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.