Prupe.1G025100_v2.0.a1

GDP-mannose 3,5-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0003420
Physical Location & Seq
Reverse (-)
1735713 .. 1739773
4061 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G025100.1

Sequence Viewer

Length: 1140 bp
ATGGGAACTACTGGGGGAAGTAAATACGGTGAATACACATATGAGAACCTGGAGAGGGAACAGTACTGGCCTTCCGAAAAGCTTCGGGTTTCCATAACGGGGGCTGGTGGTTTCATTGCTTCGCATATTGCCAGGCGACTGAAGAGTGAGGGTCACTACATTATTGCTTCTGACTGGAAGAAAAATGAACACATGACAGAGGACATGTTTTGTAATGAGTTCCATCTTGTTGATCTGAGGGTGATGGATAACTGTTTGAAGGTCACTTCTGGTGTTGACCATGTGTTCAACCTTGCTGCTGACATGGGAGGTATGGGTTTTATTCAGTCCAACCATTCCGTCATTATGTATAACAACACAATGATCAGCTTTAACATGCTCGAGGCAGCAAGGATCAATGGAGCTAAGAGGCTCTTTTATGCTTCTAGTGCTTGCATTTACCCTGAATTTAAACAGTTGGACACTAGTAATGTGAGCTTGAAGGAGTCTGATGCTTGGCCTGCGGAGCCTCAAGATGCTTATGGCTTGGAGAAGCTCATGACTGAAGAATTATGCAAGCACTATAATAAGGATTTTGGTATTGAGTGTCGGATCGGGCGGTTCCACAATATTTATGGACCCTTTGGAACTTGGAAAGGTGGTAGAGAGAAGGCCCCTGCTGCTTTTTGTCGAAAAACCCTCACTTCCACTGATAAGTTTGAGATGTGGGGAGATGGGCTACAAACGCGGTCTTTCACCTTCATTGATGAATGTGTAGAAGGTGTCCTAAGATTGACAAAGTCTGACTTCCGGGAGCCGGTAAATATTGGAAGTGATGAGATGGTTAGCATGAATGAGATGGCTGAGATAGTCCTAAGCTTTGAGAACCAGAAGCTCCCCATCCATCATATCCCTGGCCCTGAGGGTGTTCGCGGACGAAACTCAGACAACACTCTGATTAAGGAGAAGCTTGGCTGGGCCCCTACCATGAAGTTGAAGGATGGATTGAGAATTACGTACTTTTGGATTAAGGAACAGTTAGCGAAAGAGAAGGCTCAAGGCATGAATTTGTTAGGTTATGGGTCATCAAAAGTAGTGGGAACCCAAGCACCTGTTCAACTGGGCTCTCTTAGAGCTGCTGATGGGAAAGAGTTGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko00520 ko01100 ko01110 map00053 map00520 map01100 map01110
Pfam Domains
Protein Families
Epimerase

Protein Analysis

380

Amino Acids

42.72

Weight (kDa)

6.24

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 727, 912
AciI CCGC 4 cut(s) 503, 598, 727, 912
AclWI GGATC 2 cut(s) 401, 599
AcsI RAATTY 2 cut(s) 446, 1045
AcuI CTGAAG 2 cut(s) 161, 564
AfaI GTAC 2 cut(s) 65, 998
AfiI CCNNNNNNNGG 3 cut(s) 55, 99, 796
AflIII ACRYGT 1 cut(s) 204
AgsI TTSAA 5 cut(s) 259, 289, 481, 976, 1097
AhlI ACTAGT 1 cut(s) 464
AjnI CCWGG 3 cut(s) 48, 131, 892
AjuI GAANNNNNNNTTGG 2 cut(s) 1077, 1109
AluBI AGCT 9 cut(s) 82, 369, 404, 477, 535, 858, 874, 949, 1115
AluI AGCT 9 cut(s) 82, 369, 404, 477, 535, 858, 874, 949, 1115
AlwI GGATC 2 cut(s) 401, 599
Ama87I CYCGRG 1 cut(s) 380
AoxI GGCC 5 cut(s) 68, 497, 651, 895, 957
ApaI GGGCCC 1 cut(s) 961
ApeKI GCWGC 4 cut(s) 296, 386, 659, 1115
ApoI RAATTY 2 cut(s) 446, 1045
Asp700I GAANNNNTTC 1 cut(s) 81
AspS9I GGNCC 5 cut(s) 617, 652, 896, 957, 958
AsuC2I CCSGG 1 cut(s) 791
AsuHPI GGTGA 3 cut(s) 41, 253, 727
AvaI CYCGRG 1 cut(s) 380
AvaII GGWCC 1 cut(s) 617
AxyI CCTNAGG 1 cut(s) 900
BaeGI GKGCMC 1 cut(s) 961
BanII GRGCYC 2 cut(s) 961, 1106
BbvI GCAGC 4 cut(s) 283, 398, 646, 1102
BccI CCATC 9 cut(s) 231, 238, 707, 814, 832, 887, 891, 974, 1115
BciT130I CCWGG 3 cut(s) 50, 133, 894
BclI TGATCA 1 cut(s) 363
BcnI CCSGG 1 cut(s) 791
BcuI ACTAGT 1 cut(s) 464
BfaI CTAG 2 cut(s) 426, 465
BisI GCNGC 4 cut(s) 297, 387, 660, 1116
BlsI GCNGC 4 cut(s) 298, 388, 661, 1117
BmcAI AGTACT 1 cut(s) 65
Bme1390I CCNGG 4 cut(s) 50, 133, 791, 894
Bme18I GGWCC 1 cut(s) 617
BmeT110I CYCGRG 1 cut(s) 380
BmgT120I GGNCC 5 cut(s) 617, 652, 896, 957, 958
BmiI GGNNCC 8 cut(s) 507, 602, 619, 654, 795, 959, 960, 1081
BmrFI CCNGG 4 cut(s) 50, 133, 791, 894
BmrI ACTGGG 2 cut(s) 21, 1109
BmsI GCATC 2 cut(s) 481, 505
BmuI ACTGGG 2 cut(s) 21, 1109
BpmI CTGGAG 1 cut(s) 71
Bpu10I CCTNAGC 1 cut(s) 854
BpuEI CTTGAG 2 cut(s) 495, 1020
BpuMI CCSGG 1 cut(s) 791
BsaAI YACGTR 1 cut(s) 996
BsaJI CCNNGG 1 cut(s) 892
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 99, 796
Bse118I RCCGGY 1 cut(s) 796
Bse1I ACTGG 4 cut(s) 16, 71, 179, 1104
Bse21I CCTNAGG 1 cut(s) 900
Bse3DI GCAATG 1 cut(s) 114
BseBI CCWGG 3 cut(s) 50, 133, 894
BseDI CCNNGG 1 cut(s) 892
BseGI GGATG 2 cut(s) 879, 985
BseLI CCNNNNNNNGG 3 cut(s) 55, 99, 796
BseMI GCAATG 1 cut(s) 114
BseMII CTCAG 4 cut(s) 227, 834, 891, 936
BseNI ACTGG 4 cut(s) 16, 71, 179, 1104
BseSI GKGCMC 1 cut(s) 961
BseXI GCAGC 4 cut(s) 283, 398, 646, 1102
BseYI CCCAGC 1 cut(s) 954
Bsh1236I CGCG 2 cut(s) 727, 912
BshFI GGCC 5 cut(s) 70, 499, 653, 897, 959
BsiHKCI CYCGRG 1 cut(s) 380
BsiSI CCGG 2 cut(s) 790, 797
BslI CCNNNNNNNGG 3 cut(s) 55, 99, 796
BsnI GGCC 5 cut(s) 70, 499, 653, 897, 959
BsoBI CYCGRG 1 cut(s) 380
Bsp120I GGGCCC 1 cut(s) 957
Bsp1286I GDGCHC 2 cut(s) 961, 1106
Bsp143I GATC 4 cut(s) 232, 363, 393, 591
BspACI CCGC 4 cut(s) 503, 598, 727, 912
BspANI GGCC 5 cut(s) 70, 499, 653, 897, 959
BspCNI CTCAG 4 cut(s) 228, 835, 892, 935
BspFNI CGCG 2 cut(s) 727, 912
BspHI TCATGA 1 cut(s) 537
BspLI GGNNCC 8 cut(s) 507, 602, 619, 654, 795, 959, 960, 1081
BspPI GGATC 2 cut(s) 401, 599
BsrDI GCAATG 1 cut(s) 114
BsrFI RCCGGY 1 cut(s) 796
BsrI ACTGG 4 cut(s) 16, 71, 179, 1104
BssAI RCCGGY 1 cut(s) 796
BssECI CCNNGG 1 cut(s) 892
BssMI GATC 4 cut(s) 232, 363, 393, 591
Bst2UI CCWGG 3 cut(s) 50, 133, 894
Bst4CI ACNGT 5 cut(s) 29, 63, 254, 456, 1017
Bst6I CTCTTC 1 cut(s) 137
BstBAI YACGTR 1 cut(s) 996
BstC8I GCNNGC 3 cut(s) 433, 501, 557
BstDEI CTNAG 8 cut(s) 236, 405, 767, 843, 854, 900, 922, 1109
BstF5I GGATG 2 cut(s) 879, 985
BstFNI CGCG 2 cut(s) 727, 912
BstKTI GATC 4 cut(s) 235, 366, 396, 594
BstMBI GATC 4 cut(s) 232, 363, 393, 591
BstMWI GCNNNNNNNGC 5 cut(s) 428, 500, 505, 659, 724
BstNI CCWGG 3 cut(s) 50, 133, 894
BstNSI RCATGY 2 cut(s) 208, 379
BstSCI CCNGG 4 cut(s) 48, 131, 789, 892
BstSLI GKGCMC 1 cut(s) 961
BstSNI TACGTA 1 cut(s) 996
BstUI CGCG 2 cut(s) 727, 912
BstV1I GCAGC 4 cut(s) 283, 398, 646, 1102
Bsu36I CCTNAGG 1 cut(s) 900
BsuRI GGCC 5 cut(s) 70, 499, 653, 897, 959
BtsCI GGATG 2 cut(s) 879, 985
BtsIMutI CAGTG 1 cut(s) 687
Cac8I GCNNGC 3 cut(s) 433, 501, 557
CciI TCATGA 1 cut(s) 537
Cfr10I RCCGGY 1 cut(s) 796
Cfr13I GGNCC 5 cut(s) 617, 652, 896, 957, 958
Csp6I GTAC 2 cut(s) 64, 997
CspCI CAANNNNNGTGG 2 cut(s) 1056, 1091
CviAII CATG 9 cut(s) 193, 205, 281, 304, 376, 538, 829, 967, 1042
CviQI GTAC 2 cut(s) 64, 997
DdeI CTNAG 8 cut(s) 236, 405, 767, 843, 854, 900, 922, 1109
DpnI GATC 4 cut(s) 234, 365, 395, 593
DpnII GATC 4 cut(s) 232, 363, 393, 591
DraI TTTAAA 1 cut(s) 451
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
Eco105I TACGTA 1 cut(s) 996
Eco24I GRGCYC 2 cut(s) 961, 1106
Eco47I GGWCC 1 cut(s) 617
Eco57I CTGAAG 2 cut(s) 161, 564
Eco81I CCTNAGG 1 cut(s) 900
Eco88I CYCGRG 1 cut(s) 380
EcoO109I RGGNCCY 2 cut(s) 652, 958
EcoRII CCWGG 3 cut(s) 48, 131, 892
EcoT38I GRGCYC 2 cut(s) 961, 1106
FaeI CATG 9 cut(s) 196, 208, 284, 307, 379, 541, 832, 970, 1045
FalI AAGNNNNNCTT 4 cut(s) 398, 430, 770, 802
FatI CATG 9 cut(s) 192, 204, 280, 303, 375, 537, 828, 966, 1041
FauNDI CATATG 1 cut(s) 40
FbaI TGATCA 1 cut(s) 363
Fnu4HI GCNGC 4 cut(s) 297, 387, 660, 1116
FokI GGATG 2 cut(s) 866, 992
FriOI GRGCYC 2 cut(s) 961, 1106
Fsp4HI GCNGC 4 cut(s) 297, 387, 660, 1116
FspBI CTAG 2 cut(s) 426, 465
GluI GCNGC 4 cut(s) 297, 387, 660, 1116
GsaI CCCAGC 1 cut(s) 958
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 5 cut(s) 70, 499, 653, 897, 959
HapII CCGG 2 cut(s) 790, 797
Hin1II CATG 9 cut(s) 196, 208, 284, 307, 379, 541, 832, 970, 1045
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 3 cut(s) 80, 856, 947
HinfI GANTC 1 cut(s) 485
HpaII CCGG 2 cut(s) 790, 797
HphI GGTGA 3 cut(s) 41, 253, 727
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 8 cut(s) 76, 172, 237, 490, 591, 784, 925, 936
Hpy188III TCNNGA 2 cut(s) 512, 538
Hpy8I GTNNAC 1 cut(s) 277
HpyAV CCTTC 8 cut(s) 81, 253, 475, 643, 748, 752, 970, 1024
HpyCH4III ACNGT 5 cut(s) 29, 63, 254, 456, 1017
HpyCH4IV ACGT 1 cut(s) 995
HpyCH4V TGCA 2 cut(s) 435, 555
HpyF10VI GCNNNNNNNGC 5 cut(s) 428, 500, 505, 659, 724
HpyF3I CTNAG 8 cut(s) 236, 405, 767, 843, 854, 900, 922, 1109
HpySE526I ACGT 1 cut(s) 995
Hsp92II CATG 9 cut(s) 196, 208, 284, 307, 379, 541, 832, 970, 1045
Ksp22I TGATCA 1 cut(s) 363
Kzo9I GATC 4 cut(s) 232, 363, 393, 591
LmnI GCTCC 4 cut(s) 401, 505, 793, 879
Lsp1109I GCAGC 4 cut(s) 283, 398, 646, 1102
LweI GCATC 2 cut(s) 481, 505
MaeI CTAG 2 cut(s) 426, 465
MaeII ACGT 1 cut(s) 995
MaeIII GTNAC 2 cut(s) 152, 262
MalI GATC 4 cut(s) 234, 365, 395, 593
MboI GATC 4 cut(s) 232, 363, 393, 591
MboII GAAGA 3 cut(s) 154, 190, 557
MhlI GDGCHC 2 cut(s) 961, 1106
MluCI AATT 4 cut(s) 446, 548, 990, 1045
MlyI GAGTC 1 cut(s) 494
MmeI TCCRAC 3 cut(s) 354, 438, 569
MroXI GAANNNNTTC 1 cut(s) 81
MseI TTAA 4 cut(s) 372, 450, 939, 1008
MspI CCGG 2 cut(s) 790, 797
MspR9I CCNGG 4 cut(s) 50, 133, 791, 894
MvaI CCWGG 3 cut(s) 50, 133, 894
MvnI CGCG 2 cut(s) 727, 912
MwoI GCNNNNNNNGC 5 cut(s) 428, 500, 505, 659, 724
NciI CCSGG 1 cut(s) 791
NdeI CATATG 1 cut(s) 40
NdeII GATC 4 cut(s) 232, 363, 393, 591
NlaIII CATG 9 cut(s) 196, 208, 284, 307, 379, 541, 832, 970, 1045
NlaIV GGNNCC 8 cut(s) 507, 602, 619, 654, 795, 959, 960, 1081
NmuCI GTSAC 2 cut(s) 152, 262
NspI RCATGY 2 cut(s) 208, 379
PaeR7I CTCGAG 1 cut(s) 380
PagI TCATGA 1 cut(s) 537
PciI ACATGT 1 cut(s) 204
PdmI GAANNNNTTC 1 cut(s) 81
PflFI GACNNNGTC 1 cut(s) 778
PfoI TCCNGGA 1 cut(s) 789
PkrI GCNGC 4 cut(s) 298, 388, 661, 1117
PleI GAGTC 1 cut(s) 493
PpsI GAGTC 1 cut(s) 493
Ppu21I YACGTR 1 cut(s) 996
PscI ACATGT 1 cut(s) 204
Psp6I CCWGG 3 cut(s) 48, 131, 892
PspFI CCCAGC 1 cut(s) 954
PspGI CCWGG 3 cut(s) 48, 131, 892
PspN4I GGNNCC 8 cut(s) 507, 602, 619, 654, 795, 959, 960, 1081
PspOMI GGGCCC 1 cut(s) 957
PspPI GGNCC 5 cut(s) 617, 652, 896, 957, 958
PspXI VCTCGAGB 1 cut(s) 380
PsyI GACNNNGTC 1 cut(s) 778
RsaI GTAC 2 cut(s) 65, 998
RsaNI GTAC 2 cut(s) 64, 997
SaqAI TTAA 4 cut(s) 372, 450, 939, 1008
SatI GCNGC 4 cut(s) 297, 387, 660, 1116
Sau3AI GATC 4 cut(s) 232, 363, 393, 591
Sau96I GGNCC 5 cut(s) 617, 652, 896, 957, 958
ScaI AGTACT 1 cut(s) 65
SchI GAGTC 1 cut(s) 494
ScrFI CCNGG 4 cut(s) 50, 133, 791, 894
SduI GDGCHC 2 cut(s) 961, 1106
SfaNI GCATC 2 cut(s) 481, 505
Sfr274I CTCGAG 1 cut(s) 380
SinI GGWCC 1 cut(s) 617
SlaI CTCGAG 1 cut(s) 380
SmlI CTYRAG 3 cut(s) 380, 510, 1035
SmoI CTYRAG 3 cut(s) 380, 510, 1035
SnaBI TACGTA 1 cut(s) 996
SpeI ACTAGT 1 cut(s) 464
Sse9I AATT 4 cut(s) 446, 548, 990, 1045
SsiI CCGC 4 cut(s) 503, 598, 727, 912
SspI AATATT 2 cut(s) 610, 805
SspMI CTAG 2 cut(s) 426, 465
StyD4I CCNGG 4 cut(s) 48, 131, 789, 892
TaaI ACNGT 5 cut(s) 29, 63, 254, 456, 1017
TaiI ACGT 1 cut(s) 998
TaqI TCGA 2 cut(s) 381, 670
TasI AATT 4 cut(s) 446, 548, 990, 1045
TatI WGTACW 1 cut(s) 63
Tru1I TTAA 4 cut(s) 372, 450, 939, 1008
Tru9I TTAA 4 cut(s) 372, 450, 939, 1008
TscAI CASTG 1 cut(s) 694
TseFI GTSAC 2 cut(s) 152, 262
TseI GCWGC 4 cut(s) 296, 386, 659, 1115
Tsp45I GTSAC 2 cut(s) 152, 262
TspDTI ATGAA 7 cut(s) 103, 201, 730, 762, 845, 983, 1058
TspGWI ACGGA 1 cut(s) 328
TspRI CASTG 1 cut(s) 694
Tth111I GACNNNGTC 1 cut(s) 778
VpaK11BI GGWCC 1 cut(s) 617
XapI RAATTY 2 cut(s) 446, 1045
XceI RCATGY 2 cut(s) 208, 379
XcmI CCANNNNNNNNNTGG 2 cut(s) 611, 890
XhoI CTCGAG 1 cut(s) 380
XmnI GAANNNNTTC 1 cut(s) 81
XspI CTAG 2 cut(s) 426, 465
ZrmI AGTACT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.