Rh3CG033800

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0003420
Physical Location & Seq
Forward (+)
2317761 .. 2318249
489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG033800.1

Sequence Viewer

Length: 489 bp
ATGGTTGAAGTTCTCCAAAACACTGCCAAACGGAGAGGATCCAGAAAATTTGTGCAAATATCTTACACATTGAGCAGTTGCACCGTTGAATGCTTGTTTGAGACAAAAAGAATTGAGTTGGATATGTTAGTCATTACACATGCTTCTCTCCTGAAGCTTTTTGATATTGCTGATACACGGATCTTTTCAGAAATATTGACTGAATTAAGGATTTTCCATGAGAAGTTGGTTTCATTGATCAATTCACTGTCACAGGAAAACAACAAGTTTCTCATTAAGGTATCAAATACAAAGAATATCTTACCAACTGATCACTTTGAGTTGAACACCAACTTCACATTCACTTCCCCTATCCCCCAACTAGTACTGGAGGATAACAGGAGGAGGAATGTGGTGGAGAGAGTAAACCATGACATGAGCTTGATCATCCAGTGCATGCTTATGAAGGTAATGAAACCCAGTATAGGTTTTGGACCGATGGATAGGTAG

Protein Analysis

162

Amino Acids

18.8

Weight (kDa)

8.86

Isoelectric Point (pI)

56.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_AB PF26557 16 - 112 3.8e-09 Cullin alpha+beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 33, 46, 188
AcsI RAATTY 1 cut(s) 47
AcuI CTGAAG 1 cut(s) 173
AfaI GTAC 1 cut(s) 366
AfiI CCNNNNNNNGG 1 cut(s) 464
AgsI TTSAA 3 cut(s) 8, 89, 325
AhlI ACTAGT 1 cut(s) 361
AjuI GAANNNNNNNTTGG 2 cut(s) 323, 355
AluBI AGCT 2 cut(s) 157, 420
AluI AGCT 2 cut(s) 157, 420
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 3 cut(s) 33, 46, 188
ApoI RAATTY 1 cut(s) 47
AspS9I GGNCC 1 cut(s) 473
AvaII GGWCC 1 cut(s) 473
BamHI GGATCC 1 cut(s) 38
BccI CCATC 1 cut(s) 472
BclI TGATCA 3 cut(s) 237, 310, 423
BcoDI GTCTC 1 cut(s) 95
BcuI ACTAGT 1 cut(s) 361
BfaI CTAG 1 cut(s) 362
BmcAI AGTACT 1 cut(s) 366
Bme18I GGWCC 1 cut(s) 473
BmgT120I GGNCC 1 cut(s) 473
BmiI GGNNCC 1 cut(s) 40
BmrI ACTGGG 1 cut(s) 453
BmuI ACTGGG 1 cut(s) 453
BpmI CTGGAG 1 cut(s) 389
Bsc4I CCNNNNNNNGG 1 cut(s) 464
Bse1I ACTGG 3 cut(s) 372, 430, 459
BseGI GGATG 1 cut(s) 426
BseLI CCNNNNNNNGG 1 cut(s) 464
BseNI ACTGG 3 cut(s) 372, 430, 459
BseRI GAGGAG 1 cut(s) 397
BslI CCNNNNNNNGG 1 cut(s) 464
BsmAI GTCTC 1 cut(s) 95
BsmI GAATGC 1 cut(s) 95
Bsp143I GATC 5 cut(s) 38, 180, 237, 310, 423
BspLI GGNNCC 1 cut(s) 40
BspPI GGATC 3 cut(s) 33, 46, 188
BsrI ACTGG 3 cut(s) 372, 430, 459
BssMI GATC 5 cut(s) 38, 180, 237, 310, 423
Bst4CI ACNGT 2 cut(s) 85, 249
BstC8I GCNNGC 1 cut(s) 437
BstF5I GGATG 1 cut(s) 426
BstKTI GATC 5 cut(s) 41, 183, 240, 313, 426
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 5 cut(s) 38, 180, 237, 310, 423
BstNSI RCATGY 2 cut(s) 143, 439
BstX2I RGATCY 2 cut(s) 38, 180
BstYI RGATCY 2 cut(s) 38, 180
BtsCI GGATG 1 cut(s) 426
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 3 cut(s) 21, 245, 437
Cac8I GCNNGC 1 cut(s) 437
Cfr13I GGNCC 1 cut(s) 473
Csp6I GTAC 1 cut(s) 365
CviAII CATG 5 cut(s) 140, 218, 410, 415, 436
CviJI RGCY 2 cut(s) 157, 420
CviKI_1 RGCY 2 cut(s) 157, 420
CviQI GTAC 1 cut(s) 365
DpnI GATC 5 cut(s) 40, 182, 239, 312, 425
DpnII GATC 5 cut(s) 38, 180, 237, 310, 423
Eco47I GGWCC 1 cut(s) 473
Eco57I CTGAAG 1 cut(s) 173
FaeI CATG 5 cut(s) 143, 221, 413, 418, 439
FaiI YATR 8 cut(s) 125, 141, 219, 411, 416, 437, 443, 464
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FatI CATG 5 cut(s) 139, 217, 409, 414, 435
FbaI TGATCA 3 cut(s) 237, 310, 423
FokI GGATG 1 cut(s) 413
FspBI CTAG 1 cut(s) 362
GsuI CTGGAG 1 cut(s) 389
Hin1II CATG 5 cut(s) 143, 221, 413, 418, 439
HindIII AAGCTT 1 cut(s) 155
Hpy166II GTNNAC 1 cut(s) 406
Hpy188I TCNGA 1 cut(s) 190
Hpy188III TCNNGA 2 cut(s) 42, 151
Hpy8I GTNNAC 1 cut(s) 406
HpyAV CCTTC 1 cut(s) 439
HpyCH4III ACNGT 2 cut(s) 85, 249
HpyCH4V TGCA 3 cut(s) 55, 81, 435
Hsp92II CATG 5 cut(s) 143, 221, 413, 418, 439
Ksp22I TGATCA 3 cut(s) 237, 310, 423
Kzo9I GATC 5 cut(s) 38, 180, 237, 310, 423
LpnPI CCDG 7 cut(s) 55, 164, 239, 353, 364, 443, 472
MaeI CTAG 1 cut(s) 362
MaeIII GTNAC 1 cut(s) 249
MalI GATC 5 cut(s) 40, 182, 239, 312, 425
MboI GATC 5 cut(s) 38, 180, 237, 310, 423
MflI RGATCY 2 cut(s) 38, 180
MluCI AATT 4 cut(s) 47, 111, 203, 241
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 4 cut(s) 29, 364, 375, 378
MseI TTAA 2 cut(s) 206, 276
MslI CAYNNNNRTG 1 cut(s) 440
Mva1269I GAATGC 1 cut(s) 95
NdeII GATC 5 cut(s) 38, 180, 237, 310, 423
NlaIII CATG 5 cut(s) 143, 221, 413, 418, 439
NlaIV GGNNCC 1 cut(s) 40
NmuCI GTSAC 1 cut(s) 249
NspI RCATGY 2 cut(s) 143, 439
PaeI GCATGC 1 cut(s) 439
PctI GAATGC 1 cut(s) 95
PspN4I GGNNCC 1 cut(s) 40
PspPI GGNCC 1 cut(s) 473
PsuI RGATCY 2 cut(s) 38, 180
RsaI GTAC 1 cut(s) 366
RsaNI GTAC 1 cut(s) 365
RseI CAYNNNNRTG 1 cut(s) 440
SaqAI TTAA 2 cut(s) 206, 276
Sau3AI GATC 5 cut(s) 38, 180, 237, 310, 423
Sau96I GGNCC 1 cut(s) 473
ScaI AGTACT 1 cut(s) 366
SetI ASST 6 cut(s) 159, 282, 422, 450, 469, 488
SinI GGWCC 1 cut(s) 473
SmiMI CAYNNNNRTG 1 cut(s) 440
SpeI ACTAGT 1 cut(s) 361
SphI GCATGC 1 cut(s) 439
Sse9I AATT 4 cut(s) 47, 111, 203, 241
SspI AATATT 1 cut(s) 195
SspMI CTAG 1 cut(s) 362
TaaI ACNGT 2 cut(s) 85, 249
TasI AATT 4 cut(s) 47, 111, 203, 241
TatI WGTACW 1 cut(s) 364
Tru1I TTAA 2 cut(s) 206, 276
Tru9I TTAA 2 cut(s) 206, 276
TscAI CASTG 3 cut(s) 28, 252, 437
TseFI GTSAC 1 cut(s) 249
Tsp45I GTSAC 1 cut(s) 249
TspDTI ATGAA 3 cut(s) 222, 458, 467
TspGWI ACGGA 2 cut(s) 46, 193
TspRI CASTG 3 cut(s) 28, 252, 437
VpaK11BI GGWCC 1 cut(s) 473
XapI RAATTY 1 cut(s) 47
XceI RCATGY 2 cut(s) 143, 439
XspI CTAG 1 cut(s) 362
ZrmI AGTACT 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.