Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003420

Physical Location & Seq

Genomic Coordinates Reverse (-)

30432281 .. 30435747

Gene Structure 3467 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1224200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1131 bp

ATGGGAACTGCTGGTGAAACTAAGTATGGTGCGTACACTTATGAAAACCTCGAGAGGGAACCTTATTGGCCTTCTGAAAAGCTCCGAATTTCCATCACCGGGGCAGGTGGCTTTATCGCCTCCCACATTGCCCGTAGATTGAAGAATGAGGGTCATTACATCATTGCTTCTGACTGGAAGAAGAATGAGCACATGACTGAAGATATGTTCTGCCATGAATTCCATCTTGCTGACCTCAGGGTCATGGATAACTGCTTGAAGGTTACTAAGAATGTTGACCATGTGTTCAACCTCGCAGCTGATATGGGCGGAATGGGCTTCATTCAGTCCAACCATTCTGTCATCTTTTATAATAATACCATGATTAGCTTCAACATGGTTGAAGCTTCTCGGATCAATGATGTGAAGAGGTTTTTCTATGCTTCAAGTGCTTGTATTTATCCTGAATTTAAGCAGCTGGAAACCAATGTGAGCCTAAAGGAGTCTGATGCTTGGCCTGCGGAGCCTCAAGATGCTTATGGCTTGGAGAAGCTTGCAACTGAGGAATTGTGCAAGCACTACACAAAAGACTTTGGAATTGAGTGCCGTATTGGAAGGTTCCACAACATTTATGGTCCTTTTGGAACCTGGAAAGGTGGAAGGGAGAAGGCTCCTGCTGCTTTTTGCAGAAAGGCACTCACTGCCACTGATAAGTTTGAGATGTGGGGAGATGGACTTCAGACCCGATCCTTCACTTTTATTGATGAATGTGTAGAAGGTGTTCTTCGGTTGACGAAGTCAGACTTCCGTGAGCCAGTGAATATTGGAAGTGATGAGATGGTTAGCATGAATGAGATGGCTGAGATGGTTCTTAGCTTTGAGGACAAGAAGCTGCCTATCCAGCACATTCCTGGGCCAGAGGGTGTCCGTGGTCGTAACTCGGACAACACGCTGATCAAAGAGAAACTTGGCTGGGCTCCTACCATGAGGTTGAAGGATGGTCTGAGAATTACATACTTCTGGATCAAGGAACAGATTGAGAAAGAGAAGGCACAAGGTGCTGACCTCTCGGTGTATGGCTCATCTAAGGTTGTCGGAACCCAAGCCCCAGTTCAACTTGGTTCGCTGCGTGCTGCTGATGGTAAAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00053 ko00520 ko01100 ko01110 map00053 map00520 map01100 map01110

Pfam Domains

Protein Families

Epimerase

Protein Analysis

377

Amino Acids

42.59

Weight (kDa)

6.0

Isoelectric Point (pI)

39.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	30 - 269	8e-45	NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd	PF16363	32 - 330	7.7e-26	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	32 - 185	6.3e-08	3-beta hydroxysteroid dehydrogenase/isomerase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003420)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G28840 AT5G28840
fragaria_vesca	FvH4_6g02980
malus_domestica	MD04G1224100.v1.1 MD04G1224200.v1.1 MD12G1240900.v1.1
prunus_persica	Prupe.1G025100_v2.0.a1 Prupe.1G025100_v2.0.a1 Prupe.6G342800_v2.0.a1
pyrus_communis	pycom04g19750
rosa_chinensis	RchiOBHm_Chr3g0451161 RchiOBHm_Chr4g0388911
rosa_laevigata	RLG00000010008 RLG00000010011 RLG00000025681 RLG00000025682
rosa_multiflora	Rmu_co8299443.1_g000001 Rmu_sc0005486.1_g000010 Rmu_ssc0000028.1_g000003
rosa_roxburghii	Rroxscaffold_5G00335220 Rroxscaffold_6G00428630
rosa_rugosa	Rorug02G0632400 Rorug02G0632500 Rorug03G0321000 Rorug03G0321000 Rorug03G0321100
rosa_samantha	Rh3AG034900 Rh3BG035300 Rh3CG033800 Rh3CG033900 Rh3DG034900 Rh4BG018800 Rh4CG028600 Rh4DG019300
rosa_wichuraiana	Rw3G002560 Rw4G001840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	351
AarI	CACCTGC	1 cut(s)	95
AasI	GACNNNNNNGTC	1 cut(s)	239
Acc36I	ACCTGC	1 cut(s)	95
AciI	CCGC	2 cut(s)	309, 500
AclWI	GGATC	3 cut(s)	401, 720, 1010
AcsI	RAATTY	3 cut(s)	87, 218, 446
AcuI	CTGAAG	2 cut(s)	219, 701
AdeI	CACNNNGTG	1 cut(s)	1037
AfaI	GTAC	1 cut(s)	35
AfiI	CCNNNNNNNGG	2 cut(s)	55, 99
AgsI	TTSAA	8 cut(s)	142, 259, 289, 373, 383, 426, 973, 1094
AjnI	CCWGG	2 cut(s)	626, 889
AjuI	GAANNNNNNNTTGG	2 cut(s)	1074, 1106
AluBI	AGCT	8 cut(s)	82, 299, 369, 386, 457, 532, 855, 871
AluI	AGCT	8 cut(s)	82, 299, 369, 386, 457, 532, 855, 871
Alw21I	GWGCWC	1 cut(s)	192
AlwI	GGATC	3 cut(s)	401, 720, 1010
Ama87I	CYCGRG	1 cut(s)	50
AoxI	GGCC	3 cut(s)	68, 494, 893
ApeKI	GCWGC	6 cut(s)	296, 454, 656, 871, 1105, 1112
ApoI	RAATTY	3 cut(s)	87, 218, 446
Asp700I	GAANNNNTTC	1 cut(s)	759
AspS9I	GGNCC	2 cut(s)	614, 893
AsuC2I	CCSGG	1 cut(s)	100
AsuHPI	GGTGA	2 cut(s)	26, 88
AvaI	CYCGRG	1 cut(s)	50
AvaII	GGWCC	1 cut(s)	614
AxyI	CCTNAGG	1 cut(s)	236
BanII	GRGCYC	1 cut(s)	958
Bbv12I	GWGCWC	1 cut(s)	192
BbvI	GCAGC	6 cut(s)	308, 466, 643, 858, 1092, 1099
BccI	CCATC	8 cut(s)	101, 231, 704, 811, 829, 838, 971, 1112
BceAI	ACGGC	1 cut(s)	570
BciT130I	CCWGG	2 cut(s)	628, 891
BclI	TGATCA	1 cut(s)	933
BcnI	CCSGG	1 cut(s)	100
BfuAI	ACCTGC	1 cut(s)	95
BisI	GCNGC	6 cut(s)	297, 455, 657, 872, 1106, 1113
BlsI	GCNGC	6 cut(s)	298, 456, 658, 873, 1107, 1114
Bme1390I	CCNGG	3 cut(s)	100, 628, 891
Bme18I	GGWCC	1 cut(s)	614
BmeT110I	CYCGRG	1 cut(s)	50
BmgT120I	GGNCC	2 cut(s)	614, 893
BmiI	GGNNCC	7 cut(s)	60, 504, 599, 625, 651, 957, 1078
BmrFI	CCNGG	3 cut(s)	100, 628, 891
BmrI	ACTGGG	1 cut(s)	1082
BmsI	GCATC	2 cut(s)	478, 502
BmuI	ACTGGG	1 cut(s)	1082
BpuEI	CTTGAG	1 cut(s)	492
BpuMI	CCSGG	1 cut(s)	100
BsaJI	CCNNGG	3 cut(s)	99, 890, 907
Bsc4I	CCNNNNNNNGG	2 cut(s)	55, 99
Bse1I	ACTGG	3 cut(s)	179, 794, 1088
Bse21I	CCTNAGG	1 cut(s)	236
Bse3DI	GCAATG	2 cut(s)	126, 162
BseBI	CCWGG	2 cut(s)	628, 891
BseDI	CCNNGG	3 cut(s)	99, 890, 907
BseGI	GGATG	1 cut(s)	982
BseLI	CCNNNNNNNGG	2 cut(s)	55, 99
BseMI	GCAATG	2 cut(s)	126, 162
BseMII	CTCAG	4 cut(s)	250, 531, 831, 974
BseNI	ACTGG	3 cut(s)	179, 794, 1088
BseXI	GCAGC	6 cut(s)	308, 466, 643, 858, 1092, 1099
BseYI	CCCAGC	1 cut(s)	951
BshFI	GGCC	3 cut(s)	70, 496, 895
BsiHKAI	GWGCWC	1 cut(s)	192
BsiHKCI	CYCGRG	1 cut(s)	50
BsiSI	CCGG	1 cut(s)	99
BslI	CCNNNNNNNGG	2 cut(s)	55, 99
BsnI	GGCC	3 cut(s)	70, 496, 895
BsoBI	CYCGRG	1 cut(s)	50
Bsp1286I	GDGCHC	2 cut(s)	192, 958
Bsp143I	GATC	4 cut(s)	393, 725, 933, 1002
BspACI	CCGC	2 cut(s)	309, 500
BspANI	GGCC	3 cut(s)	70, 496, 895
BspCNI	CTCAG	4 cut(s)	249, 532, 832, 975
BspLI	GGNNCC	7 cut(s)	60, 504, 599, 625, 651, 957, 1078
BspMI	ACCTGC	1 cut(s)	95
BspPI	GGATC	3 cut(s)	401, 720, 1010
BsrDI	GCAATG	2 cut(s)	126, 162
BsrI	ACTGG	3 cut(s)	179, 794, 1088
BssECI	CCNNGG	3 cut(s)	99, 890, 907
BssMI	GATC	4 cut(s)	393, 725, 933, 1002
Bst2UI	CCWGG	2 cut(s)	628, 891
Bst6I	CTCTTC	1 cut(s)	401
BstAPI	GCANNNNNTGC	2 cut(s)	680, 1037
BstC8I	GCNNGC	4 cut(s)	498, 534, 554, 1110
BstDEI	CTNAG	8 cut(s)	21, 236, 267, 540, 840, 851, 983, 1065
BstDSI	CCRYGG	1 cut(s)	907
BstF5I	GGATG	1 cut(s)	982
BstKTI	GATC	4 cut(s)	396, 728, 936, 1005
BstMBI	GATC	4 cut(s)	393, 725, 933, 1002
BstMWI	GCNNNNNNNGC	8 cut(s)	315, 428, 497, 502, 656, 680, 880, 1037
BstNI	CCWGG	2 cut(s)	628, 891
BstSCI	CCNGG	3 cut(s)	98, 626, 889
BstV1I	GCAGC	6 cut(s)	308, 466, 643, 858, 1092, 1099
Bsu36I	CCTNAGG	1 cut(s)	236
BsuRI	GGCC	3 cut(s)	70, 496, 895
BtgI	CCRYGG	1 cut(s)	907
BtsCI	GGATG	1 cut(s)	982
BtsI	GCAGTG	1 cut(s)	678
BtsIMutI	CAGTG	3 cut(s)	678, 684, 801
BveI	ACCTGC	1 cut(s)	95
Cac8I	GCNNGC	4 cut(s)	498, 534, 554, 1110
Cfr13I	GGNCC	2 cut(s)	614, 893
Csp6I	GTAC	1 cut(s)	34
CviAII	CATG	8 cut(s)	193, 215, 244, 281, 361, 376, 826, 964
CviQI	GTAC	1 cut(s)	34
DdeI	CTNAG	8 cut(s)	21, 236, 267, 540, 840, 851, 983, 1065
DpnI	GATC	4 cut(s)	395, 727, 935, 1004
DpnII	GATC	4 cut(s)	393, 725, 933, 1002
DraIII	CACNNNGTG	1 cut(s)	1037
DrdI	GACNNNNNNGTC	1 cut(s)	239
DseDI	GACNNNNNNGTC	1 cut(s)	239
Eam1104I	CTCTTC	1 cut(s)	401
EarI	CTCTTC	1 cut(s)	401
EciI	GGCGGA	1 cut(s)	324
Eco24I	GRGCYC	1 cut(s)	958
Eco47I	GGWCC	1 cut(s)	614
Eco57I	CTGAAG	2 cut(s)	219, 701
Eco81I	CCTNAGG	1 cut(s)	236
Eco88I	CYCGRG	1 cut(s)	50
EcoRI	GAATTC	1 cut(s)	218
EcoRII	CCWGG	2 cut(s)	626, 889
EcoT38I	GRGCYC	1 cut(s)	958
FaeI	CATG	8 cut(s)	196, 218, 247, 284, 364, 379, 829, 967
FalI	AAGNNNNNCTT	6 cut(s)	747, 779, 767, 799, 930, 962
FatI	CATG	8 cut(s)	192, 214, 243, 280, 360, 375, 825, 963
FbaI	TGATCA	1 cut(s)	933
Fnu4HI	GCNGC	6 cut(s)	297, 455, 657, 872, 1106, 1113
FokI	GGATG	1 cut(s)	989
FriOI	GRGCYC	1 cut(s)	958
Fsp4HI	GCNGC	6 cut(s)	297, 455, 657, 872, 1106, 1113
GluI	GCNGC	6 cut(s)	297, 455, 657, 872, 1106, 1113
GsaI	CCCAGC	1 cut(s)	955
HaeIII	GGCC	3 cut(s)	70, 496, 895
HapII	CCGG	1 cut(s)	99
Hin1II	CATG	8 cut(s)	196, 218, 247, 284, 364, 379, 829, 967
HincII	GTYRAC	2 cut(s)	277, 771
HindII	GTYRAC	2 cut(s)	277, 771
HindIII	AAGCTT	2 cut(s)	384, 530
HinfI	GANTC	1 cut(s)	482
HpaII	CCGG	1 cut(s)	99
HphI	GGTGA	2 cut(s)	26, 88
Hpy166II	GTNNAC	3 cut(s)	36, 277, 771
Hpy188III	TCNNGA	4 cut(s)	52, 443, 509, 1000
Hpy8I	GTNNAC	3 cut(s)	36, 277, 771
HpyAV	CCTTC	9 cut(s)	81, 253, 588, 633, 640, 739, 749, 967, 1021
HpyCH4V	TGCA	3 cut(s)	536, 552, 666
HpyF10VI	GCNNNNNNNGC	8 cut(s)	315, 428, 497, 502, 656, 680, 880, 1037
HpyF3I	CTNAG	8 cut(s)	21, 236, 267, 540, 840, 851, 983, 1065
Hsp92II	CATG	8 cut(s)	196, 218, 247, 284, 364, 379, 829, 967
Ksp22I	TGATCA	1 cut(s)	933
Kzo9I	GATC	4 cut(s)	393, 725, 933, 1002
LmnI	GCTCC	4 cut(s)	87, 502, 655, 961
Lsp1109I	GCAGC	6 cut(s)	308, 466, 643, 858, 1092, 1099
LweI	GCATC	2 cut(s)	478, 502
MaeIII	GTNAC	2 cut(s)	262, 914
MalI	GATC	4 cut(s)	395, 727, 935, 1004
MboI	GATC	4 cut(s)	393, 725, 933, 1002
MboII	GAAGA	6 cut(s)	154, 190, 193, 212, 418, 755
MhlI	GDGCHC	2 cut(s)	192, 958
MluCI	AATT	6 cut(s)	87, 218, 446, 545, 576, 987
MlyI	GAGTC	1 cut(s)	491
MmeI	TCCRAC	2 cut(s)	354, 1054
MroXI	GAANNNNTTC	1 cut(s)	759
MseI	TTAA	1 cut(s)	450
MspA1I	CMGCKG	2 cut(s)	299, 457
MspI	CCGG	1 cut(s)	99
MspR9I	CCNGG	3 cut(s)	100, 628, 891
MvaI	CCWGG	2 cut(s)	628, 891
MwoI	GCNNNNNNNGC	8 cut(s)	315, 428, 497, 502, 656, 680, 880, 1037
NciI	CCSGG	1 cut(s)	100
NdeII	GATC	4 cut(s)	393, 725, 933, 1002
NlaIII	CATG	8 cut(s)	196, 218, 247, 284, 364, 379, 829, 967
NlaIV	GGNNCC	7 cut(s)	60, 504, 599, 625, 651, 957, 1078
PaeR7I	CTCGAG	1 cut(s)	50
PaqCI	CACCTGC	1 cut(s)	95
PdmI	GAANNNNTTC	1 cut(s)	759
PflFI	GACNNNGTC	1 cut(s)	775
PkrI	GCNGC	6 cut(s)	298, 456, 658, 873, 1107, 1114
PleI	GAGTC	1 cut(s)	490
PpsI	GAGTC	1 cut(s)	490
PsiI	TTATAA	1 cut(s)	351
Psp6I	CCWGG	2 cut(s)	626, 889
PspFI	CCCAGC	1 cut(s)	951
PspGI	CCWGG	2 cut(s)	626, 889
PspN4I	GGNNCC	7 cut(s)	60, 504, 599, 625, 651, 957, 1078
PspPI	GGNCC	2 cut(s)	614, 893
PsrI	GAACNNNNNNTAC	2 cut(s)	744, 776
PsyI	GACNNNGTC	1 cut(s)	775
PvuII	CAGCTG	2 cut(s)	299, 457
RsaI	GTAC	1 cut(s)	35
RsaNI	GTAC	1 cut(s)	34
SaqAI	TTAA	1 cut(s)	450
SatI	GCNGC	6 cut(s)	297, 455, 657, 872, 1106, 1113
Sau3AI	GATC	4 cut(s)	393, 725, 933, 1002
Sau96I	GGNCC	2 cut(s)	614, 893
SchI	GAGTC	1 cut(s)	491
ScrFI	CCNGG	3 cut(s)	100, 628, 891
SduI	GDGCHC	2 cut(s)	192, 958
SfaNI	GCATC	2 cut(s)	478, 502
Sfr274I	CTCGAG	1 cut(s)	50
SinI	GGWCC	1 cut(s)	614
SlaI	CTCGAG	1 cut(s)	50
SmlI	CTYRAG	2 cut(s)	50, 507
SmoI	CTYRAG	2 cut(s)	50, 507
Sse9I	AATT	6 cut(s)	87, 218, 446, 545, 576, 987
SsiI	CCGC	2 cut(s)	309, 500
SspI	AATATT	1 cut(s)	802
StyD4I	CCNGG	3 cut(s)	98, 626, 889
TaqI	TCGA	1 cut(s)	51
TasI	AATT	6 cut(s)	87, 218, 446, 545, 576, 987
Tru1I	TTAA	1 cut(s)	450
Tru9I	TTAA	1 cut(s)	450
TscAI	CASTG	3 cut(s)	685, 691, 801
TseI	GCWGC	6 cut(s)	296, 454, 656, 871, 1105, 1112
TspDTI	ATGAA	5 cut(s)	57, 231, 310, 759, 842
TspGWI	ACGGA	2 cut(s)	776, 896
TspRI	CASTG	3 cut(s)	685, 691, 801
Tth111I	GACNNNGTC	1 cut(s)	775
VpaK11BI	GGWCC	1 cut(s)	614
XapI	RAATTY	3 cut(s)	87, 218, 446
XcmI	CCANNNNNNNNNTGG	2 cut(s)	608, 887
XhoI	CTCGAG	1 cut(s)	50
XmnI	GAANNNNTTC	1 cut(s)	759

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.