RLG00000025682

Belongs to the cullin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003420
Physical Location & Seq
Reverse (-)
47565228 .. 47570952
5725 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025682

Sequence Viewer

Length: 3363 bp
ATGGTGGCTGGTCGGAGCTTTCAGGTTCACCACAACGGCTCAGACTTCGAAGTTAACTACCTCTCCGACTCTGGCCTCCAAGGTTTTAAAGACGAGCTTTTCCGCCTCACACATATCCCTCCCGATGAGCAAAAGATTATGGGAGCCGATGAAGATCGTATCATTTCCTGTGAATCCGATCTCCTTGCGGTGATCTCTGGGGAGCTTCGATTGGTTTCAGCCTCCGGAAACGACGGATTGCTGAGTTCCGATGAGGAAGAGGCCGGAATGTTGCAGGAAAGGGATAAACAACCCGATAATAGAGGATTAAAAGGGAAAGGGCTGGCTTATCTGACACCAACTGTTAAGGAGGGGATCCAGCATTTGGAGCTCGATAAATACAGGTTTGTTAATCAACCTTCCTGTGATGGTTCCGGCAATGACACCAGGGGTCATGGCATGCGTGTTGCAACTCCTTCAGAAATTCTTCATGGACGTTATGGAGATAAGATTCATGGGAAAATTACCAATTTTCTCCGAAAGTGTCTGCTACTAGAAGGCCCAGGAGTCGATCATATAAAAGAACTGGAACTCTTTCAACAAGATCGTCGCAGATACATTTATAGCCTCATCAGTATATGTTTCTGTGACAATCTTGACAATGATATTCGTGATATAGCTGGCTTGTGTCTAAAGAATGAACTAAAGGATGGCAAGGGTTTGGTTGACCCATGTTTGATGGACTTTATCAAAACTAAGTCGGTAGCCATCATATCTTCTCCCAGAGATTGTGTTCCTCGTTCAACATGTCAAGTCATTTCCCAACTGGCAGGAGTTGCAGGAAAGAAGTTGGAGGAATGGCGAGAGCTCATTGAACTGATAGAACGCTCATTGCATAATGAGCAGCTTCTGACACCAGCTGCTCTTGAAACTTTTGGATATGTAAGTGTGGAAGGATTGGCATTACGGCGGACAGTGGGGAAAATTCATGAAGTTCTAGCTCGTGGAATGAATTTGAAGAATAATGATTCTGTGTGCCTTGCTGCAACAAAAGCCATGTGCTACACCTTAAGTAATGTGGCGCCTTATCTTGTGTGTGAAATGGGAATTATACTGGAGGCTATATTGGAGGCTGCTGGATCTTCTAACTTGAACATAAAACAAGCAGCATTTGAGTGCTTGATTGTTCTACCTTCAAGGTTCTATGTGAAGCTGGCTATCCATCCCCCTCACCTGAAGAAGATTATGGAGTTTACTGCAGTGGCTGTAAAGGAGGGTAAGAAAGATGATGCAGTTCGAGCCATTCAGTTTTGGAGTTATCTTTTTGAGACACAGATTAATATGTTGAAGACCAACTCTGGGGTTCATAGCTTCTATCCGTACTATTTGACTGAGCATCACTTGATGGATCTCATTCCTCTGCTATTGTCACTCTCTGACGTGGAGGAAATAGAAAAGGCCAGTAAGTTTGACAGAAGAACCCTTCGTTTGTGTGTGGGGAACTTAGCAATAGCAGTTGGAGAAGATCATAAGTCTTTTGCAAATGCTGTAGAGCTTTTCATTGATGCCAACAGCACTGCTAGACACAAATATATGGCGAAGGTAATTAACAGTACTATGGGCGCCGTTGTGTCGGACATATTTGGTGAAGAAGAAGAAGCAGGCAAGGAGGGGAAGGCGGCAAATGAGATGGAGAGGGGTGAGGGTAGCCAAAAGCCAGAGATACCTGAGAAGAAAAAGGAGCTGGGACGGTGGGAGGGGCTGTTATTGACCTGGATGACGAGTATCTTTGAAGTATTACTGAAGCAAGACTTGGGGTTTCTTCGTAAGACAATGGTAGAGATAATTCTTACATACCCAGATCAGGATTCAATTTCAGATCCCACTGCACTGATGAAGGATGTATGCGCCAACTATTTTGAGTCAGGTGCTTTCTCTGATTTAATAGAGACACATGGTGAAAATTTGTTGCGGACGTTTTGCAAGAGCTGGGGTACACATTTATCTATGATTCAGCTCCTGAGGCTCTTCTTTTCTGACCGTGGCTGCCACTCCAAAGATCTGGAAACTTGGGTCCTGTTGCCGGTTTTTCCGAAGATAACAGATGCGGTGACGGAGGTAATTGAGCTTGCCTCCAAGGAGGACAAGCAGATCGACAAAAATCTACTGGTATCTGTGACAAAGATACTTATGGGTCATGGCTTTGGAAGGGCCTATGTGGGCAACATCAATGGAGTTTCTGCTCGTTCTGCTGCATCACTTGCTATGTACTGTGATAATCTACTCAAAAAGGGTGTCAGCGATGAAGAAATGGAAAAACAGCTTAACACGGTCAGTGTTGTCGCTAAATTTTACATTGACATGGTTTGCTTCAAAGAATGCTATATCAAAGGAGTTTATAGTCGCATACATGTTGTTGACTTGGAGCAAGAGAGCAGAATGCTAAAATTGGTTAGGGAGATACTATTTGGTGGAGAAGAGAAGTATTTGTCGTCCTTGAAAAGTTTGGAGCACATAGTATACTATATTGTTTCTAGTCAAAATGAGGACAAACTGAAGTTTAAAGAATATGCTGTCAACAAATTAATTAAAGATGATCGGATGGTGATGAACCTTGCAACAGCCCCTCATCATTGGTCATCTGATGATGCATCATTTCAAGATATTAAACTTCCTCAAGAGTTGGAGAACAAGGTTGAAGTTGTGAGGGGAGTCGCAAGGGGCTTTTTCCAGAATGCTCCCATACCTTTCCAATACACTCCCAAAGAGAAAGGACCCAGAAAATATGTGCAAATATCATACCTACTGAGTAGTTGCACTGTTGAATGCATGCTTGGGACAAAAAGAATTGGGTTGGATATGTCAATTGTGCAGGCGGCTCTCTTGATGCTTTTTGACATTGCTGATACATGGATCTTTTCAGAAATATTGACTGAATTAAATATTTCCAGTGAGGAGTTGGTTTCATTGATTAATTCACTGTCACAGGAAAACAGCAAGCTCCTCATGAAGGTACCAAATACAGAGAAAATCTTGCCAACTGATCGCTTTGAGTTGAACACCAACTTCACATTCACTTCCCCTATCCCCCGACCAGTACTGGAGGATAACAGGGGGAAGACTGTGGTGGAGAGAGTCAACAATGACATGAGCTTGACCATCCAGTGCATGCTTATGAAGGTAATGAAACCTAGTATAGGTTTTGGACCAATGGATAGGGAGAAGCTAGCAGGTGCAGCAATTGAAAAGTGTACTGGGTTAGAAAAATGCGGGTTTGTACCCACAAGAGCAGCTGTTGATGTCGGCATTGCAAGTCTGATCGAGAAGGAGTATTTCCTGGTGGATGAGAACGACAACAACAAGCTCTGGTTTAATGAGGAGTGA

Protein Analysis

1121

Amino Acids

125.82

Weight (kDa)

5.53

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_AB PF26557 921 - 1016 1.7e-13 Cullin alpha+beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3200
Acc36I ACCTGC 1 cut(s) 3200
Acc65I GGTACC 1 cut(s) 2992
AccB1I GGYRCC 3 cut(s) 1060, 1601, 2992
AccB7I CCANNNNNTGG 1 cut(s) 364
AccI GTMKAC 1 cut(s) 2499
AccIII TCCGGA 1 cut(s) 224
AciI CCGC 8 cut(s) 103, 188, 949, 1658, 1951, 2087, 2855, 3249
AclWI GGATC 6 cut(s) 349, 362, 1126, 1395, 1853, 2900
AcsI RAATTY 5 cut(s) 462, 963, 991, 1942, 2327
AcuI CTGAAG 4 cut(s) 441, 1235, 1802, 2555
AcyI GRCGYC 2 cut(s) 1061, 1602
AdeI CACNNNGTG 1 cut(s) 1937
AfaI GTAC 8 cut(s) 1361, 1594, 1975, 2249, 2994, 3078, 3232, 3258
AfiI CCNNNNNNNGG 6 cut(s) 364, 1338, 1843, 2062, 2989, 3176
AflII CTTAAG 1 cut(s) 1048
AflIII ACRYGT 2 cut(s) 785, 2389
AjiI CACGTC 1 cut(s) 1420
AjnI CCWGG 4 cut(s) 425, 541, 1751, 3315
AjuI GAANNNNNNNTTGG 6 cut(s) 1775, 1807, 2796, 2828, 3035, 3067
Alw21I GWGCWC 3 cut(s) 372, 849, 2493
Alw26I GTCTC 2 cut(s) 1301, 1922
AlwI GGATC 6 cut(s) 349, 362, 1126, 1395, 1853, 2900
AlwNI CAGNNNCTG 3 cut(s) 889, 1244, 1999
Aor13HI TCCGGA 1 cut(s) 224
AoxI GGCC 5 cut(s) 73, 261, 538, 1437, 2190
ApeKI GCWGC 9 cut(s) 883, 899, 1022, 1112, 1145, 2025, 2231, 3215, 3269
ApoI RAATTY 5 cut(s) 462, 963, 991, 1942, 2327
ArsI GACNNNNNNTTYG 2 cut(s) 2259, 2291
AseI ATTAAT 3 cut(s) 1317, 2564, 2952
Asp700I GAANNNNTTC 2 cut(s) 465, 573
Asp718I GGTACC 1 cut(s) 2992
AspLEI GCGC 3 cut(s) 1063, 1604, 1889
AspS9I GGNCC 5 cut(s) 539, 2053, 2190, 2753, 3185
AsuHPI GGTGA 8 cut(s) 20, 202, 1202, 1637, 1691, 1949, 2101, 2596
AsuII TTCGAA 1 cut(s) 48
AsuNHI GCTAGC 1 cut(s) 3205
AvaII GGWCC 3 cut(s) 2053, 2753, 3185
AxyI CCTNAGG 1 cut(s) 2000
BamHI GGATCC 1 cut(s) 354
BanI GGYRCC 3 cut(s) 1060, 1601, 2992
BanII GRGCYC 2 cut(s) 372, 849
BauI CACGAG 1 cut(s) 981
BbsI GAAGAC 2 cut(s) 1334, 3104
Bbv12I GWGCWC 3 cut(s) 372, 849, 2493
BbvI GCAGC 9 cut(s) 886, 895, 1009, 1099, 1157, 2012, 2218, 3227, 3281
BccI CCATC 9 cut(s) 401, 683, 712, 755, 1209, 1378, 1663, 2575, 3146
BceAI ACGGC 3 cut(s) 52, 962, 1589
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BciT130I CCWGG 4 cut(s) 427, 543, 1753, 3317
BcoDI GTCTC 2 cut(s) 1301, 1922
BfaI CTAG 6 cut(s) 533, 977, 1560, 2514, 3171, 3206
BfmI CTRYAG 2 cut(s) 1236, 1527
BfoI RGCGCY 2 cut(s) 1064, 1605
BfrI CTTAAG 1 cut(s) 1048
BfuAI ACCTGC 1 cut(s) 3200
BglII AGATCT 1 cut(s) 2038
BmcAI AGTACT 2 cut(s) 1594, 3078
Bme1390I CCNGG 4 cut(s) 427, 543, 1753, 3317
Bme18I GGWCC 3 cut(s) 2053, 2753, 3185
BmgBI CACGTC 1 cut(s) 1420
BmgT120I GGNCC 5 cut(s) 539, 2053, 2190, 2753, 3185
BmiI GGNNCC 8 cut(s) 145, 356, 412, 1062, 1603, 2054, 2755, 2994
BmrFI CCNGG 4 cut(s) 427, 543, 1753, 3317
BmrI ACTGGG 1 cut(s) 3243
BmsI GCATC 8 cut(s) 1258, 1384, 1534, 2074, 2243, 2617, 2639, 2856
BmtI GCTAGC 1 cut(s) 3209
BmuI ACTGGG 1 cut(s) 3243
BpiI GAAGAC 2 cut(s) 1334, 3104
BplI GAGNNNNNCTC 2 cut(s) 2096, 2128
BpmI CTGGAG 2 cut(s) 1115, 3101
Bpu14I TTCGAA 1 cut(s) 48
BpuEI CTTGAG 1 cut(s) 2640
BsaBI GATNNNNATC 1 cut(s) 153
BsaHI GRCGYC 2 cut(s) 1061, 1602
BsaJI CCNNGG 5 cut(s) 79, 426, 541, 2020, 2115
BsaWI WCCGGW 1 cut(s) 224
BsaXI ACNNNNNCTCC 4 cut(s) 47, 77, 1727, 1757
Bsc4I CCNNNNNNNGG 6 cut(s) 364, 1338, 1843, 2062, 2989, 3176
Bse118I RCCGGY 1 cut(s) 2062
Bse21I CCTNAGG 1 cut(s) 2000
Bse3DI GCAATG 4 cut(s) 424, 869, 2877, 3285
Bse8I GATNNNNATC 1 cut(s) 153
BseAI TCCGGA 1 cut(s) 224
BseBI CCWGG 4 cut(s) 427, 543, 1753, 3317
BseDI CCNNGG 5 cut(s) 79, 426, 541, 2020, 2115
BseGI GGATG 7 cut(s) 694, 1201, 1761, 1885, 2586, 3138, 3328
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 6 cut(s) 364, 1338, 1843, 2062, 2989, 3176
BseMI GCAATG 4 cut(s) 424, 869, 2877, 3285
BseMII CTCAG 6 cut(s) 54, 233, 1362, 1698, 1991, 2777
BseRI GAGGAG 2 cut(s) 2948, 2972
BseXI GCAGC 9 cut(s) 886, 895, 1009, 1099, 1157, 2012, 2218, 3227, 3281
BseYI CCCAGC 2 cut(s) 1723, 1968
BsgI GTGCAG 3 cut(s) 1851, 2870, 3234
BshFI GGCC 5 cut(s) 75, 263, 540, 1439, 2192
BshNI GGYRCC 3 cut(s) 1060, 1601, 2992
BsiHKAI GWGCWC 3 cut(s) 372, 849, 2493
BsiSI CCGG 4 cut(s) 225, 264, 414, 2063
BslFI GGGAC 2 cut(s) 1740, 2830
BslI CCNNNNNNNGG 6 cut(s) 364, 1338, 1843, 2062, 2989, 3176
BsmAI GTCTC 2 cut(s) 1301, 1922
BsmFI GGGAC 2 cut(s) 1740, 2830
BsmI GAATGC 4 cut(s) 2363, 2424, 2719, 2810
BsnI GGCC 5 cut(s) 75, 263, 540, 1439, 2192
Bsp119I TTCGAA 1 cut(s) 48
Bsp1286I GDGCHC 3 cut(s) 372, 849, 2493
Bsp13I TCCGGA 1 cut(s) 224
BspACI CCGC 8 cut(s) 103, 188, 949, 1658, 1951, 2087, 2855, 3249
BspANI GGCC 5 cut(s) 75, 263, 540, 1439, 2192
BspCNI CTCAG 6 cut(s) 53, 234, 1363, 1699, 1992, 2778
BspEI TCCGGA 1 cut(s) 224
BspHI TCATGA 2 cut(s) 967, 2985
BspLI GGNNCC 8 cut(s) 145, 356, 412, 1062, 1603, 2054, 2755, 2994
BspMAI CTGCAG 1 cut(s) 1240
BspMI ACCTGC 1 cut(s) 3200
BspOI GCTAGC 1 cut(s) 3209
BspPI GGATC 6 cut(s) 349, 362, 1126, 1395, 1853, 2900
BspQI GCTCTTC 1 cut(s) 2012
BspT104I TTCGAA 1 cut(s) 48
BspT107I GGYRCC 3 cut(s) 1060, 1601, 2992
BspTI CTTAAG 1 cut(s) 1048
BsrDI GCAATG 4 cut(s) 424, 869, 2877, 3285
BsrFI RCCGGY 1 cut(s) 2062
BssAI RCCGGY 1 cut(s) 2062
BssECI CCNNGG 5 cut(s) 79, 426, 541, 2020, 2115
BssNAI GTATAC 1 cut(s) 2500
BssNI GRCGYC 2 cut(s) 1061, 1602
BssSI CACGAG 1 cut(s) 981
BssT1I CCWWGG 2 cut(s) 79, 2115
Bst1107I GTATAC 1 cut(s) 2500
Bst2BI CACGAG 1 cut(s) 981
Bst2UI CCWGG 4 cut(s) 427, 543, 1753, 3317
Bst6I CTCTTC 3 cut(s) 252, 2012, 2451
BstACI GRCGYC 2 cut(s) 1061, 1602
BstAFI CTTAAG 1 cut(s) 1048
BstAPI GCANNNNNTGC 2 cut(s) 815, 2240
BstBI TTCGAA 1 cut(s) 48
BstDEI CTNAG 8 cut(s) 40, 242, 735, 1371, 1483, 1707, 2000, 2786
BstDSI CCRYGG 1 cut(s) 2020
BstENI CCTNNNNNAGG 2 cut(s) 2987, 3174
BstF5I GGATG 7 cut(s) 694, 1201, 1761, 1885, 2586, 3138, 3328
BstH2I RGCGCY 2 cut(s) 1064, 1605
BstHHI GCGC 3 cut(s) 1063, 1604, 1889
BstMAI GTCTC 2 cut(s) 1301, 1922
BstMWI GCNNNNNNNGC 9 cut(s) 367, 815, 880, 1031, 1277, 2002, 2228, 2240, 3215
BstNI CCWGG 4 cut(s) 427, 543, 1753, 3317
BstNSI RCATGY 5 cut(s) 442, 789, 2393, 2812, 3151
BstSCI CCNGG 4 cut(s) 425, 541, 1751, 3315
BstSFI CTRYAG 2 cut(s) 1236, 1527
BstV1I GCAGC 9 cut(s) 886, 895, 1009, 1099, 1157, 2012, 2218, 3227, 3281
BstV2I GAAGAC 2 cut(s) 1334, 3104
BstX2I RGATCY 6 cut(s) 354, 1118, 1387, 1858, 2038, 2892
BstXI CCANNNNNNTGG 1 cut(s) 2041
BstYI RGATCY 6 cut(s) 354, 1118, 1387, 1858, 2038, 2892
BstZ17I GTATAC 1 cut(s) 2500
Bsu36I CCTNAGG 1 cut(s) 2000
BsuRI GGCC 5 cut(s) 75, 263, 540, 1439, 2192
BtgI CCRYGG 1 cut(s) 2020
BtgZI GCGATG 1 cut(s) 2295
BtrI CACGTC 1 cut(s) 1420
BtsCI GGATG 7 cut(s) 694, 1201, 1761, 1885, 2586, 3138, 3328
BtsI GCAGTG 3 cut(s) 1245, 1554, 1863
BveI ACCTGC 1 cut(s) 3200
CaiI CAGNNNCTG 3 cut(s) 889, 1244, 1999
CciI TCATGA 2 cut(s) 967, 2985
CfoI GCGC 3 cut(s) 1063, 1604, 1889
Cfr10I RCCGGY 1 cut(s) 2062
Cfr13I GGNCC 5 cut(s) 539, 2053, 2190, 2753, 3185
Csp6I GTAC 8 cut(s) 1360, 1593, 1974, 2248, 2993, 3077, 3231, 3257
CviQI GTAC 8 cut(s) 1360, 1593, 1974, 2248, 2993, 3077, 3231, 3257
DdeI CTNAG 8 cut(s) 40, 242, 735, 1371, 1483, 1707, 2000, 2786
DinI GGCGCC 2 cut(s) 1062, 1603
DraI TTTAAA 2 cut(s) 88, 2542
DraIII CACNNNGTG 1 cut(s) 1937
Eam1104I CTCTTC 3 cut(s) 252, 2012, 2451
EarI CTCTTC 3 cut(s) 252, 2012, 2451
EciI GGCGGA 2 cut(s) 92, 964
Ecl136II GAGCTC 2 cut(s) 370, 847
Eco130I CCWWGG 2 cut(s) 79, 2115
Eco24I GRGCYC 2 cut(s) 372, 849
Eco47I GGWCC 3 cut(s) 2053, 2753, 3185
Eco53kI GAGCTC 2 cut(s) 370, 847
Eco57I CTGAAG 4 cut(s) 441, 1235, 1802, 2555
Eco81I CCTNAGG 1 cut(s) 2000
EcoICRI GAGCTC 2 cut(s) 370, 847
EcoNI CCTNNNNNAGG 2 cut(s) 2987, 3174
EcoO109I RGGNCCY 3 cut(s) 2053, 2190, 2753
EcoRII CCWGG 4 cut(s) 425, 541, 1751, 3315
EcoT14I CCWWGG 2 cut(s) 79, 2115
EcoT22I ATGCAT 2 cut(s) 2632, 2810
EcoT38I GRGCYC 2 cut(s) 372, 849
EgeI GGCGCC 2 cut(s) 1062, 1603
EheI GGCGCC 2 cut(s) 1062, 1603
ErhI CCWWGG 2 cut(s) 79, 2115
FalI AAGNNNNNCTT 4 cut(s) 81, 113, 1775, 1807
FaqI GGGAC 2 cut(s) 1740, 2830
FauI CCCGC 1 cut(s) 3242
FblI GTMKAC 1 cut(s) 2499
FokI GGATG 7 cut(s) 701, 1188, 1768, 1892, 2593, 3125, 3335
FriOI GRGCYC 2 cut(s) 372, 849
FspBI CTAG 6 cut(s) 533, 977, 1560, 2514, 3171, 3206
GlaI GCGC 3 cut(s) 1062, 1603, 1888
GsaI CCCAGC 2 cut(s) 1727, 1972
GsuI CTGGAG 2 cut(s) 1115, 3101
HaeII RGCGCY 2 cut(s) 1064, 1605
HaeIII GGCC 5 cut(s) 75, 263, 540, 1439, 2192
HapII CCGG 4 cut(s) 225, 264, 414, 2063
HhaI GCGC 3 cut(s) 1063, 1604, 1889
Hin1I GRCGYC 2 cut(s) 1061, 1602
Hin6I GCGC 3 cut(s) 1061, 1602, 1887
HinP1I GCGC 3 cut(s) 1061, 1602, 1887
HincII GTYRAC 5 cut(s) 55, 706, 2398, 2557, 3118
HindII GTYRAC 5 cut(s) 55, 706, 2398, 2557, 3118
HpaI GTTAAC 1 cut(s) 55
HpaII CCGG 4 cut(s) 225, 264, 414, 2063
HphI GGTGA 8 cut(s) 20, 202, 1202, 1637, 1691, 1949, 2101, 2596
Hpy99I CGWCG 2 cut(s) 236, 591
HpyCH4IV ACGT 3 cut(s) 475, 1419, 1955
HpyF10VI GCNNNNNNNGC 9 cut(s) 367, 815, 880, 1031, 1277, 2002, 2228, 2240, 3215
HpyF3I CTNAG 8 cut(s) 40, 242, 735, 1371, 1483, 1707, 2000, 2786
HpySE526I ACGT 3 cut(s) 475, 1419, 1955
Hsp92I GRCGYC 2 cut(s) 1061, 1602
HspAI GCGC 3 cut(s) 1061, 1602, 1887
KasI GGCGCC 2 cut(s) 1060, 1601
Kpn2I TCCGGA 1 cut(s) 224
KpnI GGTACC 1 cut(s) 2996
KspAI GTTAAC 1 cut(s) 55
LguI GCTCTTC 1 cut(s) 2012
Lsp1109I GCAGC 9 cut(s) 886, 895, 1009, 1099, 1157, 2012, 2218, 3227, 3281
LweI GCATC 8 cut(s) 1258, 1384, 1534, 2074, 2243, 2617, 2639, 2856
MaeI CTAG 6 cut(s) 533, 977, 1560, 2514, 3171, 3206
MaeII ACGT 3 cut(s) 475, 1419, 1955
MaeIII GTNAC 5 cut(s) 626, 1407, 2089, 2155, 2961
MfeI CAATTG 2 cut(s) 2844, 3219
MflI RGATCY 6 cut(s) 354, 1118, 1387, 1858, 2038, 2892
MhlI GDGCHC 3 cut(s) 372, 849, 2493
Mly113I GGCGCC 2 cut(s) 1061, 1602
MlyI GAGTC 5 cut(s) 62, 555, 1910, 2700, 3123
MmeI TCCRAC 6 cut(s) 90, 810, 1477, 1593, 2643, 2814
Mph1103I ATGCAT 2 cut(s) 2632, 2810
MroI TCCGGA 1 cut(s) 224
MroXI GAANNNNTTC 2 cut(s) 465, 573
MslI CAYNNNNRTG 3 cut(s) 1153, 2339, 3152
MspA1I CMGCKG 2 cut(s) 899, 3272
MspCI CTTAAG 1 cut(s) 1048
MspI CCGG 4 cut(s) 225, 264, 414, 2063
MspR9I CCNGG 4 cut(s) 427, 543, 1753, 3317
MunI CAATTG 2 cut(s) 2844, 3219
Mva1269I GAATGC 4 cut(s) 2363, 2424, 2719, 2810
MvaI CCWGG 4 cut(s) 427, 543, 1753, 3317
MwoI GCNNNNNNNGC 9 cut(s) 367, 815, 880, 1031, 1277, 2002, 2228, 2240, 3215
NarI GGCGCC 2 cut(s) 1061, 1602
NheI GCTAGC 1 cut(s) 3205
NlaIV GGNNCC 8 cut(s) 145, 356, 412, 1062, 1603, 2054, 2755, 2994
NmuCI GTSAC 5 cut(s) 626, 1407, 2089, 2155, 2961
NsiI ATGCAT 2 cut(s) 2632, 2810
NspI RCATGY 5 cut(s) 442, 789, 2393, 2812, 3151
NspV TTCGAA 1 cut(s) 48
PacI TTAATTAA 1 cut(s) 2568
PaeI GCATGC 3 cut(s) 442, 2812, 3151
PagI TCATGA 2 cut(s) 967, 2985
PaqCI CACCTGC 1 cut(s) 3200
PciI ACATGT 2 cut(s) 785, 2389
PciSI GCTCTTC 1 cut(s) 2012
PctI GAATGC 4 cut(s) 2363, 2424, 2719, 2810
PdmI GAANNNNTTC 2 cut(s) 465, 573
PfeI GAWTC 5 cut(s) 173, 490, 1007, 1847, 1990
PflMI CCANNNNNTGG 1 cut(s) 364
PleI GAGTC 5 cut(s) 62, 554, 1909, 2699, 3122
PluTI GGCGCC 2 cut(s) 1064, 1605
PpsI GAGTC 5 cut(s) 62, 554, 1909, 2699, 3122
PpuMI RGGWCCY 2 cut(s) 2053, 2753
PscI ACATGT 2 cut(s) 785, 2389
PshBI ATTAAT 3 cut(s) 1317, 2564, 2952
Psp124BI GAGCTC 2 cut(s) 372, 849
Psp5II RGGWCCY 2 cut(s) 2053, 2753
Psp6I CCWGG 4 cut(s) 425, 541, 1751, 3315
PspFI CCCAGC 2 cut(s) 1723, 1968
PspGI CCWGG 4 cut(s) 425, 541, 1751, 3315
PspN4I GGNNCC 8 cut(s) 145, 356, 412, 1062, 1603, 2054, 2755, 2994
PspPI GGNCC 5 cut(s) 539, 2053, 2190, 2753, 3185
PspPPI RGGWCCY 2 cut(s) 2053, 2753
PstI CTGCAG 1 cut(s) 1240
PstNI CAGNNNCTG 3 cut(s) 889, 1244, 1999
PsuI RGATCY 6 cut(s) 354, 1118, 1387, 1858, 2038, 2892
PvuII CAGCTG 2 cut(s) 899, 3272
RsaI GTAC 8 cut(s) 1361, 1594, 1975, 2249, 2994, 3078, 3232, 3258
RsaNI GTAC 8 cut(s) 1360, 1593, 1974, 2248, 2993, 3077, 3231, 3257
RseI CAYNNNNRTG 3 cut(s) 1153, 2339, 3152
SacI GAGCTC 2 cut(s) 372, 849
SapI GCTCTTC 1 cut(s) 2012
Sau96I GGNCC 5 cut(s) 539, 2053, 2190, 2753, 3185
ScaI AGTACT 2 cut(s) 1594, 3078
SchI GAGTC 5 cut(s) 62, 555, 1910, 2700, 3123
ScrFI CCNGG 4 cut(s) 427, 543, 1753, 3317
SduI GDGCHC 3 cut(s) 372, 849, 2493
SfaNI GCATC 8 cut(s) 1258, 1384, 1534, 2074, 2243, 2617, 2639, 2856
SfcI CTRYAG 2 cut(s) 1236, 1527
SfoI GGCGCC 2 cut(s) 1062, 1603
SfuI TTCGAA 1 cut(s) 48
SinI GGWCC 3 cut(s) 2053, 2753, 3185
SmiMI CAYNNNNRTG 3 cut(s) 1153, 2339, 3152
SmlI CTYRAG 2 cut(s) 1048, 2655
SmoI CTYRAG 2 cut(s) 1048, 2655
SphI GCATGC 3 cut(s) 442, 2812, 3151
SsiI CCGC 8 cut(s) 103, 188, 949, 1658, 1951, 2087, 2855, 3249
SspDI GGCGCC 2 cut(s) 1060, 1601
SspI AATATT 2 cut(s) 2907, 2923
SspMI CTAG 6 cut(s) 533, 977, 1560, 2514, 3171, 3206
SstI GAGCTC 2 cut(s) 372, 849
StyD4I CCNGG 4 cut(s) 425, 541, 1751, 3315
StyI CCWWGG 2 cut(s) 79, 2115
TaiI ACGT 3 cut(s) 478, 1422, 1958
TaqI TCGA 7 cut(s) 48, 208, 372, 549, 1276, 2133, 3300
TatI WGTACW 4 cut(s) 1592, 2247, 3076, 3230
TauI GCSGC 2 cut(s) 1661, 2858
TfiI GAWTC 5 cut(s) 173, 490, 1007, 1847, 1990
TseFI GTSAC 5 cut(s) 626, 1407, 2089, 2155, 2961
TseI GCWGC 9 cut(s) 883, 899, 1022, 1112, 1145, 2025, 2231, 3215, 3269
Tsp45I GTSAC 5 cut(s) 626, 1407, 2089, 2155, 2961
TspGWI ACGGA 3 cut(s) 249, 1347, 2108
Van91I CCANNNNNTGG 1 cut(s) 364
Vha464I CTTAAG 1 cut(s) 1048
VpaK11BI GGWCC 3 cut(s) 2053, 2753, 3185
VspI ATTAAT 3 cut(s) 1317, 2564, 2952
XagI CCTNNNNNAGG 2 cut(s) 2987, 3174
XapI RAATTY 5 cut(s) 462, 963, 991, 1942, 2327
XceI RCATGY 5 cut(s) 442, 789, 2393, 2812, 3151
XcmI CCANNNNNNNNNTGG 1 cut(s) 2935
XmiI GTMKAC 1 cut(s) 2499
XmnI GAANNNNTTC 2 cut(s) 465, 573
XspI CTAG 6 cut(s) 533, 977, 1560, 2514, 3171, 3206
ZrmI AGTACT 2 cut(s) 1594, 3078
Zsp2I ATGCAT 2 cut(s) 2632, 2810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.