Rorug03G0321000

GDP-mannose 3,5-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003420
Physical Location & Seq
Reverse (-)
34769866 .. 34771919
2054 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0321000.1

Sequence Viewer

Length: 1179 bp
ATGGCTTCTACCAATCCTCATGTTGTTTGTATTCCAGGTCCACCTCAAAGCCACGTAAAGGGAGTGCTCAAGTTTGCAAAACTCCTACACCACAGAGATGGTTTCATGGCATTGTTCACCATTACAGCCGCTGAAGAAATTGGAGCCCCCATAGTACTCTTCAATACTATTGCTGCATCCTCTTACATGGGGTTCAAACAATTTCGGACTTTGGTCCAAAAAGGGCTCGCACCATTAAAAGATGAGAGCTGTCTCACAAATGGTTTCCTGGACAAAGTAATAGATTGGATTCCCGGAATGAAAGATATTCGTTTAAAGGATCTCCCATCCTTTTTTCGAACTACAGATCCAAATGACACGATGTTTAACTTCATCATGGAAGCAACGGACAAAGTTCATAAAGCTGATGCATTCGTCTTTCTTACATTTGATGCGTTAGAACAAGATGTTTTGGACGCTATGTCATCTATATTTGATCAGTCACTTGTTTATGCAATTGGCCCTCTCGAATTACTTCTCAGTCAGATTCCGGATGGCCCTTTGAAGCATATCGGATACAGCCCGTGGAAAGAAGACATTGAGTGCTTAGAATGGCTGGAGAACAAGGCGCCAAATTCTGTTGTCTATGTGAATTTTGGAAGCATAGCAGTCTTGACACCAAAACAGCTTGTAGAGTTTGGTTGGGGCCTTGCAAATTCCAAGTTTCATTACTTCTGGGTAATTAGGCCTGATCTTGTTATTGGTGAATCAGCTATTCTGCCGCCTGAGTTGGTAGCTGAAACTAAGGAAAGAGGCCTAATAGCAAGTTGGTGCCCACAGGAGCAAGTCCTTAACCATCCATCAGTTGGTGGATTTTTAACACACTGCGGCTGGAATTCGACCATTGAGAGTCTGACGGCTGGAGTGCCTATGCTCTGTTGGCCATTCTTTGCTGACCAGCAAACAAACTGTTTCTATACTTGTAACAAATGGGGCATTGGAATGGAGATTAGCAATGATGTCAACAGAGACGAAGTAGAGAAGCTTGTTAGAGAGTTAATGGAAGGAGAGGACGGTGAGAAAATGAAAACTAAGATCATGGAGTGGAAGAAACTTGCGGAAGAAGCCACAGCTCCAGATGGTTCTTCATTCACAAACCTGGACAATTTGGTGAATCAAATTCTGTTATGGAAAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko00520 ko01100 ko01110 map00053 map00520 map01100 map01110
Pfam Domains
Protein Families
Epimerase

Protein Analysis

392

Amino Acids

44.03

Weight (kDa)

5.02

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UDPGT PF00201 124 - 361 2e-24 UDP-glucoronosyl and UDP-glucosyl transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 607, 810
AccB7I CCANNNNNTGG 1 cut(s) 845
AccIII TCCGGA 1 cut(s) 529
AciI CCGC 4 cut(s) 129, 761, 867, 1097
AclWI GGATC 2 cut(s) 327, 341
AcoI YGGCCR 1 cut(s) 920
AcsI RAATTY 5 cut(s) 613, 631, 694, 874, 1158
AcuI CTGAAG 1 cut(s) 153
AcyI GRCGYC 1 cut(s) 608
AfaI GTAC 1 cut(s) 156
AfiI CCNNNNNNNGG 2 cut(s) 58, 845
AgsI TTSAA 3 cut(s) 163, 196, 544
AhdI GACNNNNNGTC 1 cut(s) 460
AjnI CCWGG 3 cut(s) 34, 267, 1137
AloI GAACNNNNNNTCC 2 cut(s) 331, 363
AluBI AGCT 7 cut(s) 249, 404, 667, 752, 776, 1024, 1112
AluI AGCT 7 cut(s) 249, 404, 667, 752, 776, 1024, 1112
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 2 cut(s) 257, 1002
AlwI GGATC 2 cut(s) 327, 341
AlwNI CAGNNNCTG 1 cut(s) 131
Aor13HI TCCGGA 1 cut(s) 529
AoxI GGCC 6 cut(s) 499, 535, 685, 725, 793, 920
ApeKI GCWGC 1 cut(s) 173
ApoI RAATTY 5 cut(s) 613, 631, 694, 874, 1158
Asp700I GAANNNNTTC 1 cut(s) 513
AspLEI GCGC 1 cut(s) 610
AspS9I GGNCC 5 cut(s) 38, 214, 500, 536, 685
AsuC2I CCSGG 1 cut(s) 294
AsuHPI GGTGA 4 cut(s) 109, 755, 1067, 1162
AsuII TTCGAA 1 cut(s) 337
AvaII GGWCC 2 cut(s) 38, 214
BaeGI GKGCMC 1 cut(s) 815
BalI TGGCCA 1 cut(s) 922
BanI GGYRCC 2 cut(s) 607, 810
BanII GRGCYC 2 cut(s) 148, 228
BbsI GAAGAC 1 cut(s) 579
Bbv12I GWGCWC 1 cut(s) 69
BbvI GCAGC 1 cut(s) 160
BccI CCATC 6 cut(s) 92, 334, 527, 843, 847, 1112
BceAI ACGGC 1 cut(s) 912
BciT130I CCWGG 3 cut(s) 36, 269, 1139
BciVI GTATCC 1 cut(s) 548
BclI TGATCA 1 cut(s) 475
BcnI CCSGG 1 cut(s) 294
BcoDI GTCTC 2 cut(s) 257, 1002
BfmI CTRYAG 1 cut(s) 342
BfoI RGCGCY 1 cut(s) 611
BfuI GTATCC 1 cut(s) 548
BisI GCNGC 4 cut(s) 129, 174, 761, 868
BlsI GCNGC 4 cut(s) 130, 175, 762, 869
BmcAI AGTACT 1 cut(s) 156
Bme1390I CCNGG 4 cut(s) 36, 269, 294, 1139
Bme18I GGWCC 2 cut(s) 38, 214
BmeRI GACNNNNNGTC 1 cut(s) 460
BmgT120I GGNCC 5 cut(s) 38, 214, 500, 536, 685
BmiI GGNNCC 4 cut(s) 145, 609, 686, 812
BmrFI CCNGG 4 cut(s) 36, 269, 294, 1139
BmsI GCATC 3 cut(s) 185, 397, 421
BoxI GACNNNNGTC 1 cut(s) 212
BpiI GAAGAC 1 cut(s) 579
BpmI CTGGAG 3 cut(s) 617, 921, 1098
Bpu14I TTCGAA 1 cut(s) 337
BpuEI CTTGAG 1 cut(s) 53
BpuMI CCSGG 1 cut(s) 294
BsaAI YACGTR 1 cut(s) 55
BsaHI GRCGYC 1 cut(s) 608
BsaJI CCNNGG 1 cut(s) 563
BsaWI WCCGGW 1 cut(s) 529
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 845
Bse3DI GCAATG 1 cut(s) 1000
BseAI TCCGGA 1 cut(s) 529
BseBI CCWGG 3 cut(s) 36, 269, 1139
BseDI CCNNGG 1 cut(s) 563
BseGI GGATG 4 cut(s) 176, 326, 538, 835
BseLI CCNNNNNNNGG 2 cut(s) 58, 845
BseMI GCAATG 1 cut(s) 1000
BseMII CTCAG 2 cut(s) 532, 756
BseSI GKGCMC 1 cut(s) 815
BseXI GCAGC 1 cut(s) 160
BshFI GGCC 6 cut(s) 501, 537, 687, 727, 795, 922
BshNI GGYRCC 2 cut(s) 607, 810
BsiHKAI GWGCWC 1 cut(s) 69
BsiSI CCGG 2 cut(s) 294, 530
BslI CCNNNNNNNGG 2 cut(s) 58, 845
BsmAI GTCTC 2 cut(s) 257, 1002
BsmBI CGTCTC 1 cut(s) 1002
BsmI GAATGC 1 cut(s) 410
BsnI GGCC 6 cut(s) 501, 537, 687, 727, 795, 922
Bsp119I TTCGAA 1 cut(s) 337
Bsp1286I GDGCHC 4 cut(s) 69, 148, 228, 815
Bsp13I TCCGGA 1 cut(s) 529
Bsp143I GATC 5 cut(s) 319, 346, 475, 730, 1074
BspACI CCGC 4 cut(s) 129, 761, 867, 1097
BspANI GGCC 6 cut(s) 501, 537, 687, 727, 795, 922
BspCNI CTCAG 2 cut(s) 531, 757
BspEI TCCGGA 1 cut(s) 529
BspLI GGNNCC 4 cut(s) 145, 609, 686, 812
BspPI GGATC 2 cut(s) 327, 341
BspT104I TTCGAA 1 cut(s) 337
BspT107I GGYRCC 2 cut(s) 607, 810
BsrDI GCAATG 1 cut(s) 1000
BssECI CCNNGG 1 cut(s) 563
BssMI GATC 5 cut(s) 319, 346, 475, 730, 1074
BssNI GRCGYC 1 cut(s) 608
Bst2UI CCWGG 3 cut(s) 36, 269, 1139
Bst4CI ACNGT 2 cut(s) 950, 1055
Bst6I CTCTTC 1 cut(s) 164
BstACI GRCGYC 1 cut(s) 608
BstBAI YACGTR 1 cut(s) 55
BstBI TTCGAA 1 cut(s) 337
BstC8I GCNNGC 1 cut(s) 228
BstDEI CTNAG 5 cut(s) 518, 586, 765, 783, 1071
BstDSI CCRYGG 1 cut(s) 563
BstF5I GGATG 4 cut(s) 176, 326, 538, 835
BstH2I RGCGCY 1 cut(s) 611
BstHHI GCGC 1 cut(s) 610
BstKTI GATC 5 cut(s) 322, 349, 478, 733, 1077
BstMAI GTCTC 2 cut(s) 257, 1002
BstMBI GATC 5 cut(s) 319, 346, 475, 730, 1074
BstMWI GCNNNNNNNGC 2 cut(s) 919, 1103
BstNI CCWGG 3 cut(s) 36, 269, 1139
BstPAI GACNNNNGTC 1 cut(s) 212
BstSCI CCNGG 4 cut(s) 34, 267, 292, 1137
BstSFI CTRYAG 1 cut(s) 342
BstSLI GKGCMC 1 cut(s) 815
BstV1I GCAGC 1 cut(s) 160
BstV2I GAAGAC 1 cut(s) 579
BstX2I RGATCY 2 cut(s) 319, 346
BstXI CCANNNNNNTGG 1 cut(s) 98
BstYI RGATCY 2 cut(s) 319, 346
BsuI GTATCC 1 cut(s) 548
BsuRI GGCC 6 cut(s) 501, 537, 687, 727, 795, 922
BtgI CCRYGG 1 cut(s) 563
BtsCI GGATG 4 cut(s) 176, 326, 538, 835
BtsI GCAGTG 1 cut(s) 862
BtsIMutI CAGTG 1 cut(s) 862
Cac8I GCNNGC 1 cut(s) 228
CaiI CAGNNNCTG 1 cut(s) 131
CfoI GCGC 1 cut(s) 610
Cfr13I GGNCC 5 cut(s) 38, 214, 500, 536, 685
CseI GACGC 1 cut(s) 464
Csp6I GTAC 1 cut(s) 155
CviAII CATG 5 cut(s) 20, 106, 187, 376, 1078
CviQI GTAC 1 cut(s) 155
DdeI CTNAG 5 cut(s) 518, 586, 765, 783, 1071
DinI GGCGCC 1 cut(s) 609
DpnI GATC 5 cut(s) 321, 348, 477, 732, 1076
DpnII GATC 5 cut(s) 319, 346, 475, 730, 1074
DraI TTTAAA 1 cut(s) 315
DriI GACNNNNNGTC 1 cut(s) 460
EaeI YGGCCR 1 cut(s) 920
Eam1104I CTCTTC 1 cut(s) 164
Eam1105I GACNNNNNGTC 1 cut(s) 460
EarI CTCTTC 1 cut(s) 164
Eco147I AGGCCT 2 cut(s) 727, 795
Eco24I GRGCYC 2 cut(s) 148, 228
Eco47I GGWCC 2 cut(s) 38, 214
Eco57I CTGAAG 1 cut(s) 153
EcoO109I RGGNCCY 1 cut(s) 685
EcoRI GAATTC 1 cut(s) 874
EcoRII CCWGG 3 cut(s) 34, 267, 1137
EcoT22I ATGCAT 1 cut(s) 412
EcoT38I GRGCYC 2 cut(s) 148, 228
EgeI GGCGCC 1 cut(s) 609
EheI GGCGCC 1 cut(s) 609
Esp3I CGTCTC 1 cut(s) 1002
FaeI CATG 5 cut(s) 23, 109, 190, 379, 1081
FatI CATG 5 cut(s) 19, 105, 186, 375, 1077
FbaI TGATCA 1 cut(s) 475
Fnu4HI GCNGC 4 cut(s) 129, 174, 761, 868
FokI GGATG 4 cut(s) 163, 313, 545, 822
FriOI GRGCYC 2 cut(s) 148, 228
Fsp4HI GCNGC 4 cut(s) 129, 174, 761, 868
GlaI GCGC 1 cut(s) 609
GluI GCNGC 4 cut(s) 129, 174, 761, 868
GsuI CTGGAG 3 cut(s) 617, 921, 1098
HaeII RGCGCY 1 cut(s) 611
HaeIII GGCC 6 cut(s) 501, 537, 687, 727, 795, 922
HapII CCGG 2 cut(s) 294, 530
HgaI GACGC 1 cut(s) 464
HhaI GCGC 1 cut(s) 610
Hin1I GRCGYC 1 cut(s) 608
Hin1II CATG 5 cut(s) 23, 109, 190, 379, 1081
Hin6I GCGC 1 cut(s) 608
HinP1I GCGC 1 cut(s) 608
HincII GTYRAC 1 cut(s) 1003
HindII GTYRAC 1 cut(s) 1003
HindIII AAGCTT 1 cut(s) 1022
HinfI GANTC 5 cut(s) 289, 526, 746, 889, 1153
HpaII CCGG 2 cut(s) 294, 530
HphI GGTGA 4 cut(s) 109, 755, 1067, 1162
Hpy166II GTNNAC 3 cut(s) 41, 117, 1003
Hpy188I TCNGA 4 cut(s) 207, 525, 554, 894
Hpy188III TCNNGA 4 cut(s) 506, 530, 652, 1115
Hpy8I GTNNAC 3 cut(s) 41, 117, 1003
HpyAV CCTTC 1 cut(s) 1037
HpyCH4III ACNGT 2 cut(s) 950, 1055
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 5 cut(s) 77, 176, 410, 494, 692
HpyF10VI GCNNNNNNNGC 2 cut(s) 919, 1103
HpyF3I CTNAG 5 cut(s) 518, 586, 765, 783, 1071
HpySE526I ACGT 1 cut(s) 54
Hsp92I GRCGYC 1 cut(s) 608
Hsp92II CATG 5 cut(s) 23, 109, 190, 379, 1081
HspAI GCGC 1 cut(s) 608
KasI GGCGCC 1 cut(s) 607
Kpn2I TCCGGA 1 cut(s) 529
Ksp22I TGATCA 1 cut(s) 475
Kzo9I GATC 5 cut(s) 319, 346, 475, 730, 1074
LmnI GCTCC 3 cut(s) 143, 820, 1117
Lsp1109I GCAGC 1 cut(s) 160
LweI GCATC 3 cut(s) 185, 397, 421
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 2 cut(s) 480, 962
MalI GATC 5 cut(s) 321, 348, 477, 732, 1076
MboI GATC 5 cut(s) 319, 346, 475, 730, 1074
MboII GAAGA 6 cut(s) 146, 151, 584, 1099, 1112, 1116
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 2 cut(s) 319, 346
MhlI GDGCHC 4 cut(s) 69, 148, 228, 815
MlsI TGGCCA 1 cut(s) 922
MluNI TGGCCA 1 cut(s) 922
Mly113I GGCGCC 1 cut(s) 608
MlyI GAGTC 1 cut(s) 898
MnlI CCTC 6 cut(s) 27, 54, 190, 513, 785, 1042
Mox20I TGGCCA 1 cut(s) 922
Mph1103I ATGCAT 1 cut(s) 412
MroI TCCGGA 1 cut(s) 529
MroXI GAANNNNTTC 1 cut(s) 513
MscI TGGCCA 1 cut(s) 922
MseI TTAA 6 cut(s) 236, 314, 366, 831, 857, 1037
MslI CAYNNNNRTG 2 cut(s) 96, 980
Msp20I TGGCCA 1 cut(s) 922
MspA1I CMGCKG 1 cut(s) 131
MspI CCGG 2 cut(s) 294, 530
MspR9I CCNGG 4 cut(s) 36, 269, 294, 1139
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 410
MvaI CCWGG 3 cut(s) 36, 269, 1139
MwoI GCNNNNNNNGC 2 cut(s) 919, 1103
NarI GGCGCC 1 cut(s) 608
NciI CCSGG 1 cut(s) 294
NdeII GATC 5 cut(s) 319, 346, 475, 730, 1074
NlaIII CATG 5 cut(s) 23, 109, 190, 379, 1081
NlaIV GGNNCC 4 cut(s) 145, 609, 686, 812
NmuCI GTSAC 1 cut(s) 480
NsiI ATGCAT 1 cut(s) 412
NspV TTCGAA 1 cut(s) 337
PceI AGGCCT 2 cut(s) 727, 795
PctI GAATGC 1 cut(s) 410
PdmI GAANNNNTTC 1 cut(s) 513
PfeI GAWTC 4 cut(s) 289, 526, 746, 1153
PflMI CCANNNNNTGG 1 cut(s) 845
PfoI TCCNGGA 2 cut(s) 267, 292
PkrI GCNGC 4 cut(s) 130, 175, 762, 869
PleI GAGTC 1 cut(s) 897
PluTI GGCGCC 1 cut(s) 611
PpsI GAGTC 1 cut(s) 897
Ppu21I YACGTR 1 cut(s) 55
PshAI GACNNNNGTC 1 cut(s) 212
Psp6I CCWGG 3 cut(s) 34, 267, 1137
PspGI CCWGG 3 cut(s) 34, 267, 1137
PspN4I GGNNCC 4 cut(s) 145, 609, 686, 812
PspPI GGNCC 5 cut(s) 38, 214, 500, 536, 685
PstNI CAGNNNCTG 1 cut(s) 131
PsuI RGATCY 2 cut(s) 319, 346
RsaI GTAC 1 cut(s) 156
RsaNI GTAC 1 cut(s) 155
RseI CAYNNNNRTG 2 cut(s) 96, 980
SaqAI TTAA 6 cut(s) 236, 314, 366, 831, 857, 1037
SatI GCNGC 4 cut(s) 129, 174, 761, 868
Sau3AI GATC 5 cut(s) 319, 346, 475, 730, 1074
Sau96I GGNCC 5 cut(s) 38, 214, 500, 536, 685
ScaI AGTACT 1 cut(s) 156
SchI GAGTC 1 cut(s) 898
ScrFI CCNGG 4 cut(s) 36, 269, 294, 1139
SduI GDGCHC 4 cut(s) 69, 148, 228, 815
SfaNI GCATC 3 cut(s) 185, 397, 421
SfcI CTRYAG 1 cut(s) 342
SfoI GGCGCC 1 cut(s) 609
SfuI TTCGAA 1 cut(s) 337
SinI GGWCC 2 cut(s) 38, 214
SmiMI CAYNNNNRTG 2 cut(s) 96, 980
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
SseBI AGGCCT 2 cut(s) 727, 795
SsiI CCGC 4 cut(s) 129, 761, 867, 1097
SspDI GGCGCC 1 cut(s) 607
StuI AGGCCT 2 cut(s) 727, 795
StyD4I CCNGG 4 cut(s) 34, 267, 292, 1137
TaaI ACNGT 2 cut(s) 950, 1055
TaiI ACGT 1 cut(s) 57
TaqI TCGA 3 cut(s) 337, 507, 878
TatI WGTACW 1 cut(s) 154
TauI GCSGC 3 cut(s) 131, 763, 870
TfiI GAWTC 4 cut(s) 289, 526, 746, 1153
Tru1I TTAA 6 cut(s) 236, 314, 366, 831, 857, 1037
Tru9I TTAA 6 cut(s) 236, 314, 366, 831, 857, 1037
TscAI CASTG 1 cut(s) 869
TseFI GTSAC 1 cut(s) 480
TseI GCWGC 1 cut(s) 173
Tsp45I GTSAC 1 cut(s) 480
TspDTI ATGAA 7 cut(s) 94, 314, 361, 386, 695, 1079, 1116
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 1 cut(s) 869
Van91I CCANNNNNTGG 1 cut(s) 845
VpaK11BI GGWCC 2 cut(s) 38, 214
XapI RAATTY 5 cut(s) 613, 631, 694, 874, 1158
XcmI CCANNNNNNNNNTGG 1 cut(s) 842
XmnI GAANNNNTTC 1 cut(s) 513
ZrmI AGTACT 1 cut(s) 156
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.