Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003420

Physical Location & Seq

Genomic Coordinates Reverse (-)

31293938 .. 31297352

Gene Structure 3415 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1240900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1146 bp

ATGTTTTCATTTGGAATGGGAAGTACAGGCGAAATTAAGTATGGTGCGTATACCTATGAGAACCTTGAGAGGGAGCCTTATTGGCCTTCTGAAAAGCTCCGAATTTCCATTACCGGGGCAGGTGGTTTTATCGCCTCCCACATTGCCCGGAGATTGAAGAATGAGGGTCATTACATTATTGCTTCCGATTGGAAGAAGAATGAGCACATGACTGAAGACATGTTCTGCCATGAATTCCATCTTGCCGACCTCAGGGTCATGGATAATTGCTTGAAGGTTACCAAGAATGTTGACCATGTGTTCAACCTCGCAGCTGATATGGGCGGAATGGGCTTCATTCAGTCCAACCATTCTGTCATATTTTATAACAATACCATGATTAGTTTCAACATGGTCGAAGCTGCTAGGATCAATGACGTGAAGAGGTTTTTCTATGCTTCTAGTGCGTGTATTTACCCTGAGTTTAAGCAGCTGGAAACCAATGTCAGCTTGAAGGAGTCTGATGCCTGGCCTGCAGAGCCTCAAGATGCTTATGGCCTGGAGAAGCTTGCAACTGAGGAATTGTGCAAGCACTACACCAAAGACTTTGGAATCGAGTGCCGTATTGGAAGGTTCCACAACATTTATGGCCCTTTTGGAACCTGGAAAGGTGGAAGGGAGAAGGCTCCTGCTGCATTTTGCAGAAAGACTCTCACTGCCACTGATAAGTTTGAGATGTGGGGAGATGGACTTCAGACCCGATCCTTCACTTTTATTGATGAATGTGTAGAAGGTGTACTTCGGTTGACGAAGTCAGACTTCCGTGAGCCAGTGAATATTGGAAGTGATGAGATGGTTAGCATGAATGAAATGGCTGAGATCGTTCTTAGCTTTGAGGACAAGAAGCTGCCTATCCAGCACATTCCTGGGCCAGAGGGTGTCCGTGGTCGTAACTCAGACAACACACTGATCAAAGAGAAACTTGGTTGGGCTCCTACCATGAGGTTGAAGGATGGTCTGAGAATTACCTACTTCTGGATCAAGGAACAGATTGAGAAAGAGAAGGCACAAGGCGCTGACCTCTCGGTGTATGGCTCATCTAAGGTTGTGGGAACCCAAGCCCCAGTTCAACTTGGTTCGCTGCGTGCTGCTGATGGCAAAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00053 ko00520 ko01100 ko01110 map00053 map00520 map01100 map01110

Pfam Domains

Protein Families

Epimerase

Protein Analysis

382

Amino Acids

43.18

Weight (kDa)

6.0

Isoelectric Point (pI)

39.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	35 - 274	9e-45	NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd	PF16363	37 - 335	4.3e-26	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	37 - 190	3.3e-08	3-beta hydroxysteroid dehydrogenase/isomerase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003420)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G28840 AT5G28840
fragaria_vesca	FvH4_6g02980
malus_domestica	MD04G1224100.v1.1 MD04G1224200.v1.1 MD12G1240900.v1.1
prunus_persica	Prupe.1G025100_v2.0.a1 Prupe.1G025100_v2.0.a1 Prupe.6G342800_v2.0.a1
pyrus_communis	pycom04g19750
rosa_chinensis	RchiOBHm_Chr3g0451161 RchiOBHm_Chr4g0388911
rosa_laevigata	RLG00000010008 RLG00000010011 RLG00000025681 RLG00000025682
rosa_multiflora	Rmu_co8299443.1_g000001 Rmu_sc0005486.1_g000010 Rmu_ssc0000028.1_g000003
rosa_roxburghii	Rroxscaffold_5G00335220 Rroxscaffold_6G00428630
rosa_rugosa	Rorug02G0632400 Rorug02G0632500 Rorug03G0321000 Rorug03G0321000 Rorug03G0321100
rosa_samantha	Rh3AG034900 Rh3BG035300 Rh3CG033800 Rh3CG033900 Rh3DG034900 Rh4BG018800 Rh4CG028600 Rh4DG019300
rosa_wichuraiana	Rw3G002560 Rw4G001840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	366
AarI	CACCTGC	1 cut(s)	110
AasI	GACNNNNNNGTC	1 cut(s)	254
Acc36I	ACCTGC	1 cut(s)	110
AccI	GTMKAC	1 cut(s)	50
AciI	CCGC	1 cut(s)	324
AclWI	GGATC	3 cut(s)	416, 735, 1025
AcsI	RAATTY	2 cut(s)	102, 233
AcuI	CTGAAG	2 cut(s)	234, 716
AfaI	GTAC	2 cut(s)	25, 777
AfiI	CCNNNNNNNGG	4 cut(s)	70, 114, 252, 1014
AflIII	ACRYGT	1 cut(s)	219
AgsI	TTSAA	7 cut(s)	157, 274, 304, 388, 493, 988, 1109
AjiI	CACGTC	1 cut(s)	418
AjnI	CCWGG	4 cut(s)	506, 537, 641, 904
AjuI	GAANNNNNNNTTGG	2 cut(s)	1089, 1121
AluBI	AGCT	8 cut(s)	97, 314, 401, 472, 489, 547, 870, 886
AluI	AGCT	8 cut(s)	97, 314, 401, 472, 489, 547, 870, 886
Alw21I	GWGCWC	1 cut(s)	207
AlwI	GGATC	3 cut(s)	416, 735, 1025
AoxI	GGCC	5 cut(s)	83, 509, 535, 628, 908
ApeKI	GCWGC	7 cut(s)	311, 401, 469, 671, 886, 1120, 1127
ApoI	RAATTY	2 cut(s)	102, 233
AspLEI	GCGC	1 cut(s)	1055
AspS9I	GGNCC	2 cut(s)	629, 908
AsuC2I	CCSGG	2 cut(s)	115, 148
AxyI	CCTNAGG	1 cut(s)	251
BanII	GRGCYC	1 cut(s)	973
BbsI	GAAGAC	1 cut(s)	222
Bbv12I	GWGCWC	1 cut(s)	207
BbvI	GCAGC	7 cut(s)	323, 388, 481, 658, 873, 1107, 1114
BccI	CCATC	5 cut(s)	246, 719, 826, 986, 1127
BceAI	ACGGC	1 cut(s)	585
BciT130I	CCWGG	4 cut(s)	508, 539, 643, 906
BclI	TGATCA	1 cut(s)	948
BcnI	CCSGG	2 cut(s)	115, 148
BfaI	CTAG	2 cut(s)	405, 441
BfmI	CTRYAG	1 cut(s)	513
BfoI	RGCGCY	1 cut(s)	1056
BfuAI	ACCTGC	1 cut(s)	110
BglI	GCCNNNNNGGC	1 cut(s)	82
BisI	GCNGC	7 cut(s)	312, 402, 470, 672, 887, 1121, 1128
BlsI	GCNGC	7 cut(s)	313, 403, 471, 673, 888, 1122, 1129
Bme1390I	CCNGG	6 cut(s)	115, 148, 508, 539, 643, 906
BmgBI	CACGTC	1 cut(s)	418
BmgT120I	GGNCC	2 cut(s)	629, 908
BmiI	GGNNCC	6 cut(s)	75, 614, 640, 666, 972, 1093
BmrFI	CCNGG	6 cut(s)	115, 148, 508, 539, 643, 906
BmrI	ACTGGG	1 cut(s)	1097
BmsI	GCATC	2 cut(s)	493, 517
BmuI	ACTGGG	1 cut(s)	1097
BpiI	GAAGAC	1 cut(s)	222
BpmI	CTGGAG	1 cut(s)	560
BpuEI	CTTGAG	2 cut(s)	86, 507
BpuMI	CCSGG	2 cut(s)	115, 148
BsaJI	CCNNGG	3 cut(s)	114, 905, 922
Bsc4I	CCNNNNNNNGG	4 cut(s)	70, 114, 252, 1014
Bse1I	ACTGG	2 cut(s)	809, 1103
Bse21I	CCTNAGG	1 cut(s)	251
Bse3DI	GCAATG	1 cut(s)	141
BseBI	CCWGG	4 cut(s)	508, 539, 643, 906
BseDI	CCNNGG	3 cut(s)	114, 905, 922
BseGI	GGATG	1 cut(s)	997
BseLI	CCNNNNNNNGG	4 cut(s)	70, 114, 252, 1014
BseMI	GCAATG	1 cut(s)	141
BseMII	CTCAG	6 cut(s)	265, 450, 546, 846, 948, 989
BseNI	ACTGG	2 cut(s)	809, 1103
BseXI	GCAGC	7 cut(s)	323, 388, 481, 658, 873, 1107, 1114
BshFI	GGCC	5 cut(s)	85, 511, 537, 630, 910
BsiHKAI	GWGCWC	1 cut(s)	207
BsiSI	CCGG	2 cut(s)	114, 148
BslI	CCNNNNNNNGG	4 cut(s)	70, 114, 252, 1014
BsnI	GGCC	5 cut(s)	85, 511, 537, 630, 910
Bsp1286I	GDGCHC	2 cut(s)	207, 973
Bsp143I	GATC	5 cut(s)	408, 740, 858, 948, 1017
BspACI	CCGC	1 cut(s)	324
BspANI	GGCC	5 cut(s)	85, 511, 537, 630, 910
BspCNI	CTCAG	6 cut(s)	264, 451, 547, 847, 947, 990
BspLI	GGNNCC	6 cut(s)	75, 614, 640, 666, 972, 1093
BspMAI	CTGCAG	1 cut(s)	517
BspMI	ACCTGC	1 cut(s)	110
BspPI	GGATC	3 cut(s)	416, 735, 1025
BsrDI	GCAATG	1 cut(s)	141
BsrI	ACTGG	2 cut(s)	809, 1103
BssECI	CCNNGG	3 cut(s)	114, 905, 922
BssMI	GATC	5 cut(s)	408, 740, 858, 948, 1017
BssNAI	GTATAC	1 cut(s)	51
Bst1107I	GTATAC	1 cut(s)	51
Bst2UI	CCWGG	4 cut(s)	508, 539, 643, 906
Bst6I	CTCTTC	1 cut(s)	416
BstC8I	GCNNGC	4 cut(s)	513, 549, 569, 1125
BstDEI	CTNAG	8 cut(s)	251, 459, 555, 855, 866, 934, 998, 1080
BstDSI	CCRYGG	1 cut(s)	922
BstEII	GGTNACC	1 cut(s)	277
BstF5I	GGATG	1 cut(s)	997
BstH2I	RGCGCY	1 cut(s)	1056
BstHHI	GCGC	1 cut(s)	1055
BstKTI	GATC	5 cut(s)	411, 743, 861, 951, 1020
BstMBI	GATC	5 cut(s)	408, 740, 858, 948, 1017
BstMWI	GCNNNNNNNGC	8 cut(s)	82, 330, 443, 512, 517, 671, 895, 1052
BstNI	CCWGG	4 cut(s)	508, 539, 643, 906
BstNSI	RCATGY	1 cut(s)	223
BstPI	GGTNACC	1 cut(s)	277
BstSCI	CCNGG	6 cut(s)	113, 146, 506, 537, 641, 904
BstSFI	CTRYAG	1 cut(s)	513
BstV1I	GCAGC	7 cut(s)	323, 388, 481, 658, 873, 1107, 1114
BstV2I	GAAGAC	1 cut(s)	222
BstZ17I	GTATAC	1 cut(s)	51
Bsu36I	CCTNAGG	1 cut(s)	251
BsuRI	GGCC	5 cut(s)	85, 511, 537, 630, 910
BtgI	CCRYGG	1 cut(s)	922
BtrI	CACGTC	1 cut(s)	418
BtsCI	GGATG	1 cut(s)	997
BtsI	GCAGTG	1 cut(s)	693
BtsIMutI	CAGTG	4 cut(s)	693, 699, 816, 944
BveI	ACCTGC	1 cut(s)	110
Cac8I	GCNNGC	4 cut(s)	513, 549, 569, 1125
CfoI	GCGC	1 cut(s)	1055
Cfr13I	GGNCC	2 cut(s)	629, 908
Csp6I	GTAC	2 cut(s)	24, 776
CviAII	CATG	9 cut(s)	208, 220, 230, 259, 296, 376, 391, 841, 979
CviQI	GTAC	2 cut(s)	24, 776
DdeI	CTNAG	8 cut(s)	251, 459, 555, 855, 866, 934, 998, 1080
DpnI	GATC	5 cut(s)	410, 742, 860, 950, 1019
DpnII	GATC	5 cut(s)	408, 740, 858, 948, 1017
DrdI	GACNNNNNNGTC	1 cut(s)	254
DseDI	GACNNNNNNGTC	1 cut(s)	254
Eam1104I	CTCTTC	1 cut(s)	416
EarI	CTCTTC	1 cut(s)	416
EciI	GGCGGA	1 cut(s)	339
Eco24I	GRGCYC	1 cut(s)	973
Eco57I	CTGAAG	2 cut(s)	234, 716
Eco81I	CCTNAGG	1 cut(s)	251
Eco91I	GGTNACC	1 cut(s)	277
EcoO65I	GGTNACC	1 cut(s)	277
EcoRI	GAATTC	1 cut(s)	233
EcoRII	CCWGG	4 cut(s)	506, 537, 641, 904
EcoT38I	GRGCYC	1 cut(s)	973
FaeI	CATG	9 cut(s)	211, 223, 233, 262, 299, 379, 394, 844, 982
FalI	AAGNNNNNCTT	6 cut(s)	762, 794, 782, 814, 945, 977
FatI	CATG	9 cut(s)	207, 219, 229, 258, 295, 375, 390, 840, 978
FbaI	TGATCA	1 cut(s)	948
FblI	GTMKAC	1 cut(s)	50
Fnu4HI	GCNGC	7 cut(s)	312, 402, 470, 672, 887, 1121, 1128
FokI	GGATG	1 cut(s)	1004
FriOI	GRGCYC	1 cut(s)	973
Fsp4HI	GCNGC	7 cut(s)	312, 402, 470, 672, 887, 1121, 1128
FspBI	CTAG	2 cut(s)	405, 441
GlaI	GCGC	1 cut(s)	1054
GluI	GCNGC	7 cut(s)	312, 402, 470, 672, 887, 1121, 1128
GsuI	CTGGAG	1 cut(s)	560
HaeII	RGCGCY	1 cut(s)	1056
HaeIII	GGCC	5 cut(s)	85, 511, 537, 630, 910
HapII	CCGG	2 cut(s)	114, 148
HhaI	GCGC	1 cut(s)	1055
Hin1II	CATG	9 cut(s)	211, 223, 233, 262, 299, 379, 394, 844, 982
Hin6I	GCGC	1 cut(s)	1053
HinP1I	GCGC	1 cut(s)	1053
HincII	GTYRAC	2 cut(s)	292, 786
HindII	GTYRAC	2 cut(s)	292, 786
HindIII	AAGCTT	1 cut(s)	545
HinfI	GANTC	3 cut(s)	497, 591, 688
HpaII	CCGG	2 cut(s)	114, 148
Hpy166II	GTNNAC	4 cut(s)	51, 292, 776, 786
Hpy188I	TCNGA	8 cut(s)	91, 101, 187, 502, 735, 796, 937, 999
Hpy188III	TCNNGA	2 cut(s)	524, 1015
Hpy8I	GTNNAC	4 cut(s)	51, 292, 776, 786
HpyCH4IV	ACGT	1 cut(s)	417
HpyCH4V	TGCA	5 cut(s)	515, 551, 567, 674, 681
HpyF10VI	GCNNNNNNNGC	8 cut(s)	82, 330, 443, 512, 517, 671, 895, 1052
HpyF3I	CTNAG	8 cut(s)	251, 459, 555, 855, 866, 934, 998, 1080
HpySE526I	ACGT	1 cut(s)	417
Hsp92II	CATG	9 cut(s)	211, 223, 233, 262, 299, 379, 394, 844, 982
HspAI	GCGC	1 cut(s)	1053
Ksp22I	TGATCA	1 cut(s)	948
Kzo9I	GATC	5 cut(s)	408, 740, 858, 948, 1017
LmnI	GCTCC	4 cut(s)	73, 102, 670, 976
Lsp1109I	GCAGC	7 cut(s)	323, 388, 481, 658, 873, 1107, 1114
LweI	GCATC	2 cut(s)	493, 517
MaeI	CTAG	2 cut(s)	405, 441
MaeII	ACGT	1 cut(s)	417
MaeIII	GTNAC	2 cut(s)	277, 929
MalI	GATC	5 cut(s)	410, 742, 860, 950, 1019
MboI	GATC	5 cut(s)	408, 740, 858, 948, 1017
MboII	GAAGA	5 cut(s)	169, 205, 208, 227, 433
MhlI	GDGCHC	2 cut(s)	207, 973
MluCI	AATT	6 cut(s)	33, 102, 233, 265, 560, 1002
MlyI	GAGTC	2 cut(s)	506, 682
MmeI	TCCRAC	1 cut(s)	369
MseI	TTAA	2 cut(s)	36, 465
MspA1I	CMGCKG	2 cut(s)	314, 472
MspI	CCGG	2 cut(s)	114, 148
MspR9I	CCNGG	6 cut(s)	115, 148, 508, 539, 643, 906
MvaI	CCWGG	4 cut(s)	508, 539, 643, 906
MwoI	GCNNNNNNNGC	8 cut(s)	82, 330, 443, 512, 517, 671, 895, 1052
NciI	CCSGG	2 cut(s)	115, 148
NdeII	GATC	5 cut(s)	408, 740, 858, 948, 1017
NlaIII	CATG	9 cut(s)	211, 223, 233, 262, 299, 379, 394, 844, 982
NlaIV	GGNNCC	6 cut(s)	75, 614, 640, 666, 972, 1093
NspI	RCATGY	1 cut(s)	223
PaqCI	CACCTGC	1 cut(s)	110
PciI	ACATGT	1 cut(s)	219
PfeI	GAWTC	1 cut(s)	591
PflFI	GACNNNGTC	1 cut(s)	790
PkrI	GCNGC	7 cut(s)	313, 403, 471, 673, 888, 1122, 1129
PleI	GAGTC	2 cut(s)	505, 682
PpsI	GAGTC	2 cut(s)	505, 682
PscI	ACATGT	1 cut(s)	219
PsiI	TTATAA	1 cut(s)	366
Psp6I	CCWGG	4 cut(s)	506, 537, 641, 904
PspEI	GGTNACC	1 cut(s)	277
PspGI	CCWGG	4 cut(s)	506, 537, 641, 904
PspN4I	GGNNCC	6 cut(s)	75, 614, 640, 666, 972, 1093
PspPI	GGNCC	2 cut(s)	629, 908
PstI	CTGCAG	1 cut(s)	517
PsyI	GACNNNGTC	1 cut(s)	790
PvuII	CAGCTG	2 cut(s)	314, 472
RsaI	GTAC	2 cut(s)	25, 777
RsaNI	GTAC	2 cut(s)	24, 776
SaqAI	TTAA	2 cut(s)	36, 465
SatI	GCNGC	7 cut(s)	312, 402, 470, 672, 887, 1121, 1128
Sau3AI	GATC	5 cut(s)	408, 740, 858, 948, 1017
Sau96I	GGNCC	2 cut(s)	629, 908
SchI	GAGTC	2 cut(s)	506, 682
ScrFI	CCNGG	6 cut(s)	115, 148, 508, 539, 643, 906
SduI	GDGCHC	2 cut(s)	207, 973
SfaNI	GCATC	2 cut(s)	493, 517
SfcI	CTRYAG	1 cut(s)	513
SmlI	CTYRAG	2 cut(s)	65, 522
SmoI	CTYRAG	2 cut(s)	65, 522
Sse9I	AATT	6 cut(s)	33, 102, 233, 265, 560, 1002
SsiI	CCGC	1 cut(s)	324
SspI	AATATT	1 cut(s)	817
SspMI	CTAG	2 cut(s)	405, 441
StyD4I	CCNGG	6 cut(s)	113, 146, 506, 537, 641, 904
TaiI	ACGT	1 cut(s)	420
TaqI	TCGA	2 cut(s)	396, 594
TasI	AATT	6 cut(s)	33, 102, 233, 265, 560, 1002
TatI	WGTACW	2 cut(s)	23, 775
TfiI	GAWTC	1 cut(s)	591
Tru1I	TTAA	2 cut(s)	36, 465
Tru9I	TTAA	2 cut(s)	36, 465
TscAI	CASTG	4 cut(s)	700, 706, 816, 951
TseI	GCWGC	7 cut(s)	311, 401, 469, 671, 886, 1120, 1127
TspDTI	ATGAA	5 cut(s)	246, 325, 774, 857, 861
TspGWI	ACGGA	2 cut(s)	791, 911
TspRI	CASTG	4 cut(s)	700, 706, 816, 951
Tth111I	GACNNNGTC	1 cut(s)	790
XapI	RAATTY	2 cut(s)	102, 233
XceI	RCATGY	1 cut(s)	223
XcmI	CCANNNNNNNNNTGG	2 cut(s)	623, 902
XmiI	GTMKAC	1 cut(s)	50
XspI	CTAG	2 cut(s)	405, 441

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.