AT5G35460

Protein of unknown function (DUF2838)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006796
Physical Location & Seq
Forward (+)
13672463 .. 13675503
3041 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G35460.1

Sequence Viewer

Length: 1146 bp
ATGGCGAATAACGAAGATTCAAACTCCAATGGACTAGACTCGTTTGATGCTGTTAAACAACGTTTCAAAGATCGATCAAAGAAAGTTGTTCAGACGAGAGAGTTGTTGTCTAAACAAGCGGTACAAACGCGTGAGATCTTATCGAAACAAGCCGTTAAGATCGCTAAACAAGCCGAAGAACATGAGAGATTCATCAATAAGGTGACACATCTCGTTGGTGTGCTTGGATTTGGAGGTTTTTGTTTCCTACTTGGTGCAAGACCTCAAGACATTCCTTTAGTTTACTGTTTCTTCTATGTGATCTTTGTTCCTCTTCGATGGATATATTACCGGTTTAAGAAATGGCATTATTATCTTCTGGACTTTTGCTACTATGCCAATACAATCTTCTTGGTTGATCTTCTTCTGTATCCTAAGAATGAAAAGCTTTTCATGGTTTGCTTTTCCTTTGCTGAGGGACCATTGGCGTGGGCGATTATTGTCTGGCGTTGCAGTCTGGTTTTTAGTTCGCCGGATAAGATTGTTAGTGTTCTTATACATCTCTTACCTGGGCTCGTGTTTTTCACGATTAGGTGGTGGAATCCAGCGACTTTTGCAGCTATGCACCCTGTAGGCACTGATCGTAGGGTTTCATGGCCTTATGTTGAAGACAAAGCTTACCTATTCACATGGCTGTTTCTCGTCCCCTTAGTCGTTTATACTCTTTGGCAAGTGCTCTATTTTCTAATCGTCAATGTCCTGCGTAGGCAGAGACTCCTTAGAGATCCTGAAGTTATGACTTCTTACAGAGAGCTGTCGAAGAAAGCTGAAAAGGCAAACAATAAGTTGTGGCAGCTGAGCGGTCTGCTAGGGGATCAGAATCGTATATGGATGTACATCCTATTCCAGGCCATCTTCACGGTGGCAACCATGGCATTGACCGTACCGATCTTCCTATCTTATAGACTTCATGTGATCTTTCAGATCCTAAAGATTTCTGCGGCTGTGTGGAACGGAGGGAGCTTTTTACTAGAGGTAATGCCACGGCAAGTGATCCAGAAAGAGAAGAAGAAGAAAGCTGAGATGCAGCCGATAGAGGAGCAGATACTACATCATGAAGCTGTGTCACATCCGACTGAGAATGAGCCAAAATCTACTGAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2838

Protein Analysis

381

Amino Acids

44.7

Weight (kDa)

9.4

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2838 PF10998 67 - 176 2e-38 Protein of unknown function (DUF2838)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 840
AccII CGCG 1 cut(s) 130
AciI CCGC 3 cut(s) 119, 840, 980
AclI AACGTT 1 cut(s) 61
AclWI GGATC 4 cut(s) 758, 861, 958, 1027
AcuI CTGAAG 1 cut(s) 789
AfaI GTAC 3 cut(s) 123, 875, 924
AfiI CCNNNNNNNGG 1 cut(s) 886
AflIII ACRYGT 1 cut(s) 128
AgeI ACCGGT 1 cut(s) 330
AgsI TTSAA 3 cut(s) 21, 67, 647
AjnI CCWGG 2 cut(s) 547, 885
AjuI GAANNNNNNNTTGG 2 cut(s) 371, 403
AluBI AGCT 9 cut(s) 427, 599, 656, 793, 806, 835, 1002, 1058, 1100
AluI AGCT 9 cut(s) 427, 599, 656, 793, 806, 835, 1002, 1058, 1100
Alw21I GWGCWC 1 cut(s) 717
Alw26I GTCTC 1 cut(s) 745
AlwI GGATC 4 cut(s) 758, 861, 958, 1027
AoxI GGCC 2 cut(s) 635, 888
ApeKI GCWGC 3 cut(s) 596, 832, 1066
AsiGI ACCGGT 1 cut(s) 330
AspS9I GGNCC 1 cut(s) 458
AsuHPI GGTGA 1 cut(s) 214
AvaII GGWCC 1 cut(s) 458
BanII GRGCYC 1 cut(s) 555
BauI CACGAG 1 cut(s) 554
BbsI GAAGAC 1 cut(s) 654
Bbv12I GWGCWC 1 cut(s) 717
BbvCI CCTCAGC 1 cut(s) 453
BbvI GCAGC 3 cut(s) 608, 844, 1078
BccI CCATC 2 cut(s) 312, 899
BceAI ACGGC 2 cut(s) 137, 1040
BciT130I CCWGG 2 cut(s) 549, 887
BciVI GTATCC 1 cut(s) 420
BcoDI GTCTC 1 cut(s) 745
BfaI CTAG 3 cut(s) 35, 848, 1010
BfmI CTRYAG 1 cut(s) 609
BfuI GTATCC 1 cut(s) 420
BglII AGATCT 1 cut(s) 135
BisI GCNGC 4 cut(s) 597, 833, 981, 1067
BlpI GCTNAGC 1 cut(s) 836
BlsI GCNGC 4 cut(s) 598, 834, 982, 1068
Bme1390I CCNGG 2 cut(s) 549, 887
Bme18I GGWCC 1 cut(s) 458
BmgT120I GGNCC 1 cut(s) 458
BmiI GGNNCC 1 cut(s) 459
BmrFI CCNGG 2 cut(s) 549, 887
BmsI GCATC 2 cut(s) 37, 1053
BpiI GAAGAC 1 cut(s) 654
Bpu10I CCTNAGC 1 cut(s) 453
Bpu1102I GCTNAGC 1 cut(s) 836
BpuEI CTTGAG 1 cut(s) 249
Bsa29I ATCGAT 1 cut(s) 73
BsaBI GATNNNNATC 2 cut(s) 858, 875
BsaJI CCNNGG 3 cut(s) 548, 909, 1022
BsaWI WCCGGW 1 cut(s) 330
Bsc4I CCNNNNNNNGG 1 cut(s) 886
Bse118I RCCGGY 1 cut(s) 330
Bse8I GATNNNNATC 2 cut(s) 858, 875
BseBI CCWGG 2 cut(s) 549, 887
BseCI ATCGAT 1 cut(s) 73
BseDI CCNNGG 3 cut(s) 548, 909, 1022
BseGI GGATG 3 cut(s) 876, 876, 1108
BseJI GATNNNNATC 2 cut(s) 858, 875
BseLI CCNNNNNNNGG 1 cut(s) 886
BseMII CTCAG 4 cut(s) 444, 827, 1050, 1107
BseRI GAGGAG 1 cut(s) 1091
BseXI GCAGC 3 cut(s) 608, 844, 1078
Bsh1236I CGCG 1 cut(s) 130
BshFI GGCC 2 cut(s) 637, 890
BshTI ACCGGT 1 cut(s) 330
BshVI ATCGAT 1 cut(s) 73
BsiHKAI GWGCWC 1 cut(s) 717
BsiSI CCGG 2 cut(s) 331, 512
BslFI GGGAC 2 cut(s) 471, 668
BslI CCNNNNNNNGG 1 cut(s) 886
BsmAI GTCTC 1 cut(s) 745
BsmFI GGGAC 2 cut(s) 471, 668
BsnI GGCC 2 cut(s) 637, 890
Bsp1286I GDGCHC 2 cut(s) 555, 717
Bsp1407I TGTACA 1 cut(s) 873
Bsp1720I GCTNAGC 1 cut(s) 836
Bsp19I CCATGG 1 cut(s) 909
BspACI CCGC 3 cut(s) 119, 840, 980
BspANI GGCC 2 cut(s) 637, 890
BspCNI CTCAG 4 cut(s) 445, 828, 1051, 1108
BspDI ATCGAT 1 cut(s) 73
BspFNI CGCG 1 cut(s) 130
BspHI TCATGA 1 cut(s) 1093
BspLI GGNNCC 1 cut(s) 459
BspPI GGATC 4 cut(s) 758, 861, 958, 1027
BsrBI CCGCTC 1 cut(s) 840
BsrFI RCCGGY 1 cut(s) 330
BsrGI TGTACA 1 cut(s) 873
BssAI RCCGGY 1 cut(s) 330
BssECI CCNNGG 3 cut(s) 548, 909, 1022
BssSI CACGAG 1 cut(s) 554
BssT1I CCWWGG 1 cut(s) 909
Bst2BI CACGAG 1 cut(s) 554
Bst2UI CCWGG 2 cut(s) 549, 887
Bst4CI ACNGT 3 cut(s) 287, 901, 922
Bst6I CTCTTC 1 cut(s) 318
BstAUI TGTACA 1 cut(s) 873
BstDEI CTNAG 7 cut(s) 414, 453, 688, 758, 836, 1059, 1116
BstDSI CCRYGG 2 cut(s) 909, 1022
BstENI CCTNNNNNAGG 1 cut(s) 884
BstF5I GGATG 3 cut(s) 876, 876, 1108
BstFNI CGCG 1 cut(s) 130
BstMAI GTCTC 1 cut(s) 745
BstMWI GCNNNNNNNGC 4 cut(s) 170, 593, 812, 911
BstNI CCWGG 2 cut(s) 549, 887
BstSCI CCNGG 2 cut(s) 547, 885
BstSFI CTRYAG 1 cut(s) 609
BstUI CGCG 1 cut(s) 130
BstV1I GCAGC 3 cut(s) 608, 844, 1078
BstV2I GAAGAC 1 cut(s) 654
BstX2I RGATCY 3 cut(s) 135, 763, 963
BstXI CCANNNNNNTGG 1 cut(s) 468
BstYI RGATCY 3 cut(s) 135, 763, 963
Bsu15I ATCGAT 1 cut(s) 73
BsuI GTATCC 1 cut(s) 420
BsuRI GGCC 2 cut(s) 637, 890
BsuTUI ATCGAT 1 cut(s) 73
BtgI CCRYGG 2 cut(s) 909, 1022
BtsCI GGATG 3 cut(s) 876, 876, 1108
BtsIMutI CAGTG 1 cut(s) 615
CciI TCATGA 1 cut(s) 1093
Cfr10I RCCGGY 1 cut(s) 330
Cfr13I GGNCC 1 cut(s) 458
ClaI ATCGAT 1 cut(s) 73
Csp6I GTAC 3 cut(s) 122, 874, 923
CspAI ACCGGT 1 cut(s) 330
CviAII CATG 8 cut(s) 182, 433, 633, 669, 910, 950, 1094, 1143
CviQI GTAC 3 cut(s) 122, 874, 923
DdeI CTNAG 7 cut(s) 414, 453, 688, 758, 836, 1059, 1116
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 909
Eco24I GRGCYC 1 cut(s) 555
Eco47I GGWCC 1 cut(s) 458
Eco57I CTGAAG 1 cut(s) 789
EcoNI CCTNNNNNAGG 1 cut(s) 884
EcoRII CCWGG 2 cut(s) 547, 885
EcoT14I CCWWGG 1 cut(s) 909
EcoT38I GRGCYC 1 cut(s) 555
ErhI CCWWGG 1 cut(s) 909
FaeI CATG 8 cut(s) 185, 436, 636, 672, 913, 953, 1097, 1146
FaqI GGGAC 2 cut(s) 471, 668
FatI CATG 8 cut(s) 181, 432, 632, 668, 909, 949, 1093, 1142
Fnu4HI GCNGC 4 cut(s) 597, 833, 981, 1067
FokI GGATG 3 cut(s) 863, 883, 1095
FriOI GRGCYC 1 cut(s) 555
Fsp4HI GCNGC 4 cut(s) 597, 833, 981, 1067
FspBI CTAG 3 cut(s) 35, 848, 1010
GluI GCNGC 4 cut(s) 597, 833, 981, 1067
HaeIII GGCC 2 cut(s) 637, 890
HapII CCGG 2 cut(s) 331, 512
Hin1II CATG 8 cut(s) 185, 436, 636, 672, 913, 953, 1097, 1146
HindIII AAGCTT 2 cut(s) 425, 654
HinfI GANTC 6 cut(s) 17, 38, 189, 580, 753, 859
HpaII CCGG 2 cut(s) 331, 512
HphI GGTGA 1 cut(s) 214
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 4 cut(s) 93, 858, 963, 1113
Hpy188III TCNNGA 6 cut(s) 266, 359, 565, 767, 1036, 1094
Hpy8I GTNNAC 1 cut(s) 283
HpyCH4III ACNGT 3 cut(s) 287, 901, 922
HpyCH4IV ACGT 1 cut(s) 61
HpyCH4V TGCA 5 cut(s) 257, 492, 596, 604, 1066
HpyF10VI GCNNNNNNNGC 4 cut(s) 170, 593, 812, 911
HpyF3I CTNAG 7 cut(s) 414, 453, 688, 758, 836, 1059, 1116
HpySE526I ACGT 1 cut(s) 61
Hsp92II CATG 8 cut(s) 185, 436, 636, 672, 913, 953, 1097, 1146
LmnI GCTCC 2 cut(s) 999, 1078
Lsp1109I GCAGC 3 cut(s) 608, 844, 1078
LweI GCATC 2 cut(s) 37, 1053
MaeI CTAG 3 cut(s) 35, 848, 1010
MaeII ACGT 1 cut(s) 61
MaeIII GTNAC 2 cut(s) 202, 1104
MbiI CCGCTC 1 cut(s) 840
MflI RGATCY 3 cut(s) 135, 763, 963
MhlI GDGCHC 2 cut(s) 555, 717
MluI ACGCGT 1 cut(s) 128
MlyI GAGTC 2 cut(s) 32, 747
MmeI TCCRAC 1 cut(s) 1136
MnlI CCTC 7 cut(s) 227, 273, 321, 448, 989, 1006, 1069
MseI TTAA 3 cut(s) 54, 156, 336
MslI CAYNNNNRTG 1 cut(s) 466
MspA1I CMGCKG 1 cut(s) 835
MspI CCGG 2 cut(s) 331, 512
MspR9I CCNGG 2 cut(s) 549, 887
MvaI CCWGG 2 cut(s) 549, 887
MvnI CGCG 1 cut(s) 130
MwoI GCNNNNNNNGC 4 cut(s) 170, 593, 812, 911
NcoI CCATGG 1 cut(s) 909
NlaIII CATG 8 cut(s) 185, 436, 636, 672, 913, 953, 1097, 1146
NlaIV GGNNCC 1 cut(s) 459
NmuCI GTSAC 2 cut(s) 202, 1104
PagI TCATGA 1 cut(s) 1093
PfeI GAWTC 4 cut(s) 17, 189, 580, 859
PinAI ACCGGT 1 cut(s) 330
PkrI GCNGC 4 cut(s) 598, 834, 982, 1068
PleI GAGTC 2 cut(s) 32, 747
PpsI GAGTC 2 cut(s) 32, 747
Psp1406I AACGTT 1 cut(s) 61
Psp6I CCWGG 2 cut(s) 547, 885
PspGI CCWGG 2 cut(s) 547, 885
PspN4I GGNNCC 1 cut(s) 459
PspPI GGNCC 1 cut(s) 458
PsuI RGATCY 3 cut(s) 135, 763, 963
PvuII CAGCTG 1 cut(s) 835
RsaI GTAC 3 cut(s) 123, 875, 924
RsaNI GTAC 3 cut(s) 122, 874, 923
RseI CAYNNNNRTG 1 cut(s) 466
SaqAI TTAA 3 cut(s) 54, 156, 336
SatI GCNGC 4 cut(s) 597, 833, 981, 1067
Sau96I GGNCC 1 cut(s) 458
SchI GAGTC 2 cut(s) 32, 747
ScrFI CCNGG 2 cut(s) 549, 887
SduI GDGCHC 2 cut(s) 555, 717
SfaNI GCATC 2 cut(s) 37, 1053
SfcI CTRYAG 1 cut(s) 609
SinI GGWCC 1 cut(s) 458
SmiMI CAYNNNNRTG 1 cut(s) 466
SmlI CTYRAG 1 cut(s) 264
SmoI CTYRAG 1 cut(s) 264
SsiI CCGC 3 cut(s) 119, 840, 980
SspMI CTAG 3 cut(s) 35, 848, 1010
StyD4I CCNGG 2 cut(s) 547, 885
StyI CCWWGG 1 cut(s) 909
TaaI ACNGT 3 cut(s) 287, 901, 922
TaiI ACGT 1 cut(s) 64
TaqI TCGA 4 cut(s) 73, 143, 316, 797
TatI WGTACW 1 cut(s) 873
TauI GCSGC 1 cut(s) 983
TfiI GAWTC 4 cut(s) 17, 189, 580, 859
Tru1I TTAA 3 cut(s) 54, 156, 336
Tru9I TTAA 3 cut(s) 54, 156, 336
TscAI CASTG 1 cut(s) 622
TseFI GTSAC 2 cut(s) 202, 1104
TseI GCWGC 3 cut(s) 596, 832, 1066
Tsp45I GTSAC 2 cut(s) 202, 1104
TspDTI ATGAA 6 cut(s) 181, 421, 435, 621, 938, 1110
TspGWI ACGGA 1 cut(s) 1008
TspRI CASTG 1 cut(s) 622
VpaK11BI GGWCC 1 cut(s) 458
XagI CCTNNNNNAGG 1 cut(s) 884
XcmI CCANNNNNNNNNTGG 1 cut(s) 898
XspI CTAG 3 cut(s) 35, 848, 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.