AT5G37478

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005732
Physical Location & Seq
Forward (+)
14881416 .. 14883994
2579 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G37478.1

Sequence Viewer

Length: 537 bp
ATGTGTACTGGAAAATCATCATACACAAAATCCTCTCCCAAGTTTGGGAAAACAAGTCCTACTTCTTCTGTACTCTCCCTGGGTTCCAATGGTAAAGGAATGACCAAAGTGGAACCACTCAAAGAAAGAGCCCATGACAAAACCAAGGCAGCCACGACTAAGAATATTACAAAGGCACCAGCAAAAGAGAACAAAAAACCTCTCGAGTTCAAGCTTCATTCTGGCGAGAGAGCAGTGAAACGTGCAATGTTCAACTATTCGGTTGCAACTAATTATTACATCCAGAAACTTCAGAAAAAACAAGAAGAGAGGTTGCAAAAGATGATAGAAGAGGAAGAGATTCGCATGTTGAGGAAGGAAATGGTTCCAAAAGCTCAATTGATGCCTTTCTTCGACAGACCTTTTCTCCCACAAAGATCGAGCAGACCATTGACGATGCCAAAAGAACCAAGCTTTGGAAACGTAAACAGCACTTGTTGGACTTGTGTTTTCAACAACCAACACTATCTTTACCATATTAACCATGCTCATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

178

Amino Acids

20.43

Weight (kDa)

9.92

Isoelectric Point (pI)

63.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 70 - 145 2.5e-24 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 175
AccB7I CCANNNNNTGG 1 cut(s) 455
AcuI CTGAAG 1 cut(s) 275
AfaI GTAC 2 cut(s) 7, 72
AfiI CCNNNNNNNGG 3 cut(s) 44, 45, 455
AgsI TTSAA 3 cut(s) 211, 253, 493
AjnI CCWGG 1 cut(s) 78
AjuI GAANNNNNNNTTGG 2 cut(s) 438, 470
AluBI AGCT 3 cut(s) 214, 374, 453
AluI AGCT 3 cut(s) 214, 374, 453
Ama87I CYCGRG 1 cut(s) 203
ApeKI GCWGC 1 cut(s) 149
Asp700I GAANNNNTTC 2 cut(s) 339, 363
AvaI CYCGRG 1 cut(s) 203
BanI GGYRCC 1 cut(s) 175
BanII GRGCYC 1 cut(s) 133
BbvI GCAGC 1 cut(s) 161
BciT130I CCWGG 1 cut(s) 80
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
Bme1390I CCNGG 1 cut(s) 80
BmeT110I CYCGRG 1 cut(s) 203
BmiI GGNNCC 4 cut(s) 85, 114, 177, 366
BmrFI CCNGG 1 cut(s) 80
BmsI GCATC 2 cut(s) 372, 426
BsaJI CCNNGG 3 cut(s) 78, 79, 144
BsaXI ACNNNNNCTCC 2 cut(s) 390, 420
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 45, 455
Bse1I ACTGG 1 cut(s) 13
Bse3DI GCAATG 1 cut(s) 252
BseBI CCWGG 1 cut(s) 80
BseDI CCNNGG 3 cut(s) 78, 79, 144
BseGI GGATG 1 cut(s) 279
BseLI CCNNNNNNNGG 3 cut(s) 44, 45, 455
BseMI GCAATG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 13
BseXI GCAGC 1 cut(s) 161
BshNI GGYRCC 1 cut(s) 175
BsiHKCI CYCGRG 1 cut(s) 203
BslI CCNNNNNNNGG 3 cut(s) 44, 45, 455
BsoBI CYCGRG 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 133
Bsp143I GATC 1 cut(s) 416
BspLI GGNNCC 4 cut(s) 85, 114, 177, 366
BspT107I GGYRCC 1 cut(s) 175
BsrDI GCAATG 1 cut(s) 252
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 3 cut(s) 78, 79, 144
BssMI GATC 1 cut(s) 416
BssT1I CCWWGG 1 cut(s) 144
Bst2UI CCWGG 1 cut(s) 80
Bst6I CTCTTC 3 cut(s) 300, 324, 330
BstDEI CTNAG 1 cut(s) 159
BstF5I GGATG 1 cut(s) 279
BstKTI GATC 1 cut(s) 419
BstMBI GATC 1 cut(s) 416
BstNI CCWGG 1 cut(s) 80
BstNSI RCATGY 1 cut(s) 349
BstSCI CCNGG 1 cut(s) 78
BstV1I GCAGC 1 cut(s) 161
BtsCI GGATG 1 cut(s) 279
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 240
Csp6I GTAC 2 cut(s) 6, 71
CviAII CATG 4 cut(s) 134, 346, 524, 530
CviJI RGCY 5 cut(s) 131, 152, 214, 374, 453
CviKI_1 RGCY 5 cut(s) 131, 152, 214, 374, 453
CviQI GTAC 2 cut(s) 6, 71
DdeI CTNAG 1 cut(s) 159
DpnI GATC 1 cut(s) 418
DpnII GATC 1 cut(s) 416
Eam1104I CTCTTC 3 cut(s) 300, 324, 330
EarI CTCTTC 3 cut(s) 300, 324, 330
Eco130I CCWWGG 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 275
Eco88I CYCGRG 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 144
EcoT38I GRGCYC 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 4 cut(s) 137, 349, 527, 533
FaiI YATR 6 cut(s) 22, 135, 347, 516, 525, 531
FalI AAGNNNNNCTT 2 cut(s) 46, 78
FatI CATG 4 cut(s) 133, 345, 523, 529
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 1 cut(s) 266
FriOI GRGCYC 1 cut(s) 133
Fsp4HI GCNGC 1 cut(s) 150
GluI GCNGC 1 cut(s) 150
Hin1II CATG 4 cut(s) 137, 349, 527, 533
HindIII AAGCTT 2 cut(s) 212, 451
HinfI GANTC 1 cut(s) 340
Hpy166II GTNNAC 2 cut(s) 6, 466
Hpy188I TCNGA 1 cut(s) 294
Hpy188III TCNNGA 2 cut(s) 203, 283
Hpy8I GTNNAC 2 cut(s) 6, 466
HpyAV CCTTC 1 cut(s) 349
HpyCH4IV ACGT 2 cut(s) 241, 462
HpyCH4V TGCA 3 cut(s) 245, 266, 316
HpyF3I CTNAG 1 cut(s) 159
HpySE526I ACGT 2 cut(s) 241, 462
Hsp92II CATG 4 cut(s) 137, 349, 527, 533
Kzo9I GATC 1 cut(s) 416
LpnPI CCDG 5 cut(s) 65, 92, 192, 207, 296
Lsp1109I GCAGC 1 cut(s) 161
LweI GCATC 2 cut(s) 372, 426
MaeII ACGT 2 cut(s) 241, 462
MalI GATC 1 cut(s) 418
MboI GATC 1 cut(s) 416
MboII GAAGA 5 cut(s) 57, 317, 341, 347, 382
MfeI CAATTG 1 cut(s) 377
MhlI GDGCHC 1 cut(s) 133
MluCI AATT 2 cut(s) 271, 377
MmeI TCCRAC 1 cut(s) 458
MnlI CCTC 5 cut(s) 43, 210, 303, 325, 345
MroXI GAANNNNTTC 2 cut(s) 339, 363
MseI TTAA 2 cut(s) 519, 535
MslI CAYNNNNRTG 1 cut(s) 528
MspR9I CCNGG 1 cut(s) 80
MunI CAATTG 1 cut(s) 377
MvaI CCWGG 1 cut(s) 80
NdeII GATC 1 cut(s) 416
NlaIII CATG 4 cut(s) 137, 349, 527, 533
NlaIV GGNNCC 4 cut(s) 85, 114, 177, 366
NspI RCATGY 1 cut(s) 349
PaeR7I CTCGAG 1 cut(s) 203
PasI CCCWGGG 1 cut(s) 79
PdmI GAANNNNTTC 2 cut(s) 339, 363
PfeI GAWTC 1 cut(s) 340
PflMI CCANNNNNTGG 1 cut(s) 455
PkrI GCNGC 1 cut(s) 151
Psp6I CCWGG 1 cut(s) 78
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 4 cut(s) 85, 114, 177, 366
RsaI GTAC 2 cut(s) 7, 72
RsaNI GTAC 2 cut(s) 6, 71
RseI CAYNNNNRTG 1 cut(s) 528
SaqAI TTAA 2 cut(s) 519, 535
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 1 cut(s) 416
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 1 cut(s) 133
SetI ASST 8 cut(s) 202, 216, 244, 314, 376, 403, 455, 465
SfaNI GCATC 2 cut(s) 372, 426
Sfr274I CTCGAG 1 cut(s) 203
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 1 cut(s) 528
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 2 cut(s) 271, 377
SspI AATATT 1 cut(s) 166
StyD4I CCNGG 1 cut(s) 78
StyI CCWWGG 1 cut(s) 144
TaiI ACGT 2 cut(s) 244, 465
TaqI TCGA 3 cut(s) 204, 393, 419
TasI AATT 2 cut(s) 271, 377
TatI WGTACW 2 cut(s) 5, 70
TfiI GAWTC 1 cut(s) 340
Tru1I TTAA 2 cut(s) 519, 535
Tru9I TTAA 2 cut(s) 519, 535
TscAI CASTG 1 cut(s) 240
TseI GCWGC 1 cut(s) 149
TspDTI ATGAA 1 cut(s) 206
TspRI CASTG 1 cut(s) 240
Van91I CCANNNNNTGG 1 cut(s) 455
XceI RCATGY 1 cut(s) 349
XhoI CTCGAG 1 cut(s) 203
XmnI GAANNNNTTC 2 cut(s) 339, 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.