Rroxscaffold_6G00393660

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005732
Physical Location & Seq
Forward (+)
13791560 .. 13794422
2863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00393660.1

Sequence Viewer

Length: 531 bp
ATGGAGAAGGCACTCACCAAGTCTAGTCTTAAGAGTACTAGTCCTTCACACTCTGCTGTGAGTTCATGGAGCTCAAATTCTGGAGGAATGCCAAGAGATGAACTCAAAGAACGGGGATATGACAAAGACTACTACTATAAGGCTTCTTATCAGAGCAAGTCTCCTACAAGAGAAAGTACCAAGCCTCAAGAAGTTAAACTTCACACCCAACAAAGGGCAACCAAACGGGCAATGTTCAACTACACGGTTGCAGCAAAGCTGAGTATCTTGGAGCAGCGGAGAAAGCAAGAAGAGATGTTGCATAAGATGATAGAAGAGGAAGAGATTCGCTTACTGAGAAAGGAGATGATTCCAAGGGCTCAACTCATGCCGTTTTTCGATAGACCTTTTATTCCTCAAAGATCAAGCAGGCCTTTGACAATTCCTAAAGAACCAAGCTTCCGCATGATGAGCAGCAAGAGCTTCACTGCTTGTAGATCTGGTACCCCAATCTACAGTTTCCAACACACTACTCAAGCCCCCATCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

176

Amino Acids

20.46

Weight (kDa)

10.0

Isoelectric Point (pI)

73.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 66 - 140 2.9e-22 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 482
AccB1I GGYRCC 1 cut(s) 482
AciI CCGC 2 cut(s) 277, 442
AcsI RAATTY 1 cut(s) 76
AfaI GTAC 3 cut(s) 37, 178, 484
AfiI CCNNNNNNNGG 2 cut(s) 213, 214
AflII CTTAAG 1 cut(s) 29
AgsI TTSAA 1 cut(s) 238
AhlI ACTAGT 1 cut(s) 38
AloI GAACNNNNNNTCC 2 cut(s) 423, 455
AluBI AGCT 4 cut(s) 72, 259, 438, 462
AluI AGCT 4 cut(s) 72, 259, 438, 462
Alw21I GWGCWC 1 cut(s) 74
Alw26I GTCTC 1 cut(s) 165
AoxI GGCC 1 cut(s) 410
ApeKI GCWGC 3 cut(s) 251, 274, 453
ApoI RAATTY 1 cut(s) 76
Asp700I GAANNNNTTC 1 cut(s) 324
Asp718I GGTACC 1 cut(s) 482
AsuHPI GGTGA 1 cut(s) 7
BanI GGYRCC 1 cut(s) 482
BanII GRGCYC 2 cut(s) 74, 361
BarI GAAGNNNNNNTAC 2 cut(s) 28, 60
Bbv12I GWGCWC 1 cut(s) 74
BbvI GCAGC 3 cut(s) 263, 286, 465
BccI CCATC 1 cut(s) 530
BceAI ACGGC 1 cut(s) 355
BcoDI GTCTC 1 cut(s) 165
BcuI ACTAGT 1 cut(s) 38
BfaI CTAG 2 cut(s) 24, 39
BfmI CTRYAG 1 cut(s) 493
BfrI CTTAAG 1 cut(s) 29
BglII AGATCT 1 cut(s) 476
BisI GCNGC 3 cut(s) 252, 275, 454
BlsI GCNGC 3 cut(s) 253, 276, 455
BmcAI AGTACT 1 cut(s) 37
BmiI GGNNCC 1 cut(s) 484
BplI GAGNNNNNCTC 4 cut(s) 87, 119, 145, 177
BpmI CTGGAG 1 cut(s) 102
BpuEI CTTGAG 2 cut(s) 171, 498
BsaJI CCNNGG 1 cut(s) 353
Bsc4I CCNNNNNNNGG 2 cut(s) 213, 214
Bse3DI GCAATG 1 cut(s) 237
BseDI CCNNGG 1 cut(s) 353
BseLI CCNNNNNNNGG 2 cut(s) 213, 214
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 2 cut(s) 251, 326
BseXI GCAGC 3 cut(s) 263, 286, 465
BshFI GGCC 1 cut(s) 412
BshNI GGYRCC 1 cut(s) 482
BsiHKAI GWGCWC 1 cut(s) 74
BslI CCNNNNNNNGG 2 cut(s) 213, 214
BsmAI GTCTC 1 cut(s) 165
BsmI GAATGC 1 cut(s) 93
BsnI GGCC 1 cut(s) 412
Bsp1286I GDGCHC 2 cut(s) 74, 361
Bsp143I GATC 2 cut(s) 401, 476
BspACI CCGC 2 cut(s) 277, 442
BspANI GGCC 1 cut(s) 412
BspCNI CTCAG 2 cut(s) 252, 327
BspLI GGNNCC 1 cut(s) 484
BspT107I GGYRCC 1 cut(s) 482
BspTI CTTAAG 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 237
BssECI CCNNGG 1 cut(s) 353
BssMI GATC 2 cut(s) 401, 476
BssT1I CCWWGG 1 cut(s) 353
Bst4CI ACNGT 2 cut(s) 247, 497
Bst6I CTCTTC 3 cut(s) 285, 309, 315
BstAFI CTTAAG 1 cut(s) 29
BstC8I GCNNGC 1 cut(s) 410
BstDEI CTNAG 2 cut(s) 260, 335
BstKTI GATC 2 cut(s) 404, 479
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 2 cut(s) 401, 476
BstMWI GCNNNNNNNGC 3 cut(s) 283, 450, 459
BstSFI CTRYAG 1 cut(s) 493
BstV1I GCAGC 3 cut(s) 263, 286, 465
BstX2I RGATCY 1 cut(s) 476
BstYI RGATCY 1 cut(s) 476
BsuRI GGCC 1 cut(s) 412
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 1 cut(s) 465
Cac8I GCNNGC 1 cut(s) 410
Csp6I GTAC 3 cut(s) 36, 177, 483
CviAII CATG 3 cut(s) 66, 367, 445
CviJI RGCY 9 cut(s) 72, 143, 184, 259, 359, 412, 438, 462, 518
CviKI_1 RGCY 9 cut(s) 72, 143, 184, 259, 359, 412, 438, 462, 518
CviQI GTAC 3 cut(s) 36, 177, 483
DdeI CTNAG 2 cut(s) 260, 335
DpnI GATC 2 cut(s) 403, 478
DpnII GATC 2 cut(s) 401, 476
Eam1104I CTCTTC 3 cut(s) 285, 309, 315
EarI CTCTTC 3 cut(s) 285, 309, 315
Ecl136II GAGCTC 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 353
Eco147I AGGCCT 1 cut(s) 412
Eco24I GRGCYC 2 cut(s) 74, 361
Eco53kI GAGCTC 1 cut(s) 72
EcoICRI GAGCTC 1 cut(s) 72
EcoT14I CCWWGG 1 cut(s) 353
EcoT38I GRGCYC 2 cut(s) 74, 361
ErhI CCWWGG 1 cut(s) 353
FaeI CATG 3 cut(s) 69, 370, 448
FaiI YATR 6 cut(s) 67, 120, 138, 303, 368, 446
FalI AAGNNNNNCTT 4 cut(s) 183, 215, 397, 429
FatI CATG 3 cut(s) 65, 366, 444
Fnu4HI GCNGC 3 cut(s) 252, 275, 454
FriOI GRGCYC 2 cut(s) 74, 361
Fsp4HI GCNGC 3 cut(s) 252, 275, 454
FspBI CTAG 2 cut(s) 24, 39
GluI GCNGC 3 cut(s) 252, 275, 454
GsuI CTGGAG 1 cut(s) 102
HaeIII GGCC 1 cut(s) 412
Hin1II CATG 3 cut(s) 69, 370, 448
HindIII AAGCTT 1 cut(s) 436
HinfI GANTC 2 cut(s) 325, 349
HphI GGTGA 1 cut(s) 7
Hpy188I TCNGA 1 cut(s) 153
Hpy188III TCNNGA 2 cut(s) 81, 188
HpyAV CCTTC 1 cut(s) 54
HpyCH4III ACNGT 2 cut(s) 247, 497
HpyCH4V TGCA 2 cut(s) 251, 301
HpyF10VI GCNNNNNNNGC 3 cut(s) 283, 450, 459
HpyF3I CTNAG 2 cut(s) 260, 335
Hsp92II CATG 3 cut(s) 69, 370, 448
KpnI GGTACC 1 cut(s) 486
Kzo9I GATC 2 cut(s) 401, 476
LmnI GCTCC 2 cut(s) 69, 271
LpnPI CCDG 3 cut(s) 66, 394, 465
Lsp1109I GCAGC 3 cut(s) 263, 286, 465
MaeI CTAG 2 cut(s) 24, 39
MalI GATC 2 cut(s) 403, 478
MboI GATC 2 cut(s) 401, 476
MboII GAAGA 3 cut(s) 302, 326, 332
MflI RGATCY 1 cut(s) 476
MhlI GDGCHC 2 cut(s) 74, 361
MluCI AATT 2 cut(s) 76, 420
MmeI TCCRAC 1 cut(s) 526
MnlI CCTC 4 cut(s) 77, 195, 310, 405
MroXI GAANNNNTTC 1 cut(s) 324
MseI TTAA 2 cut(s) 30, 195
MspA1I CMGCKG 1 cut(s) 277
MspCI CTTAAG 1 cut(s) 29
Mva1269I GAATGC 1 cut(s) 93
MwoI GCNNNNNNNGC 3 cut(s) 283, 450, 459
NdeII GATC 2 cut(s) 401, 476
NlaIII CATG 3 cut(s) 69, 370, 448
NlaIV GGNNCC 1 cut(s) 484
PceI AGGCCT 1 cut(s) 412
PctI GAATGC 1 cut(s) 93
PdmI GAANNNNTTC 1 cut(s) 324
PfeI GAWTC 2 cut(s) 325, 349
PkrI GCNGC 3 cut(s) 253, 276, 455
Psp124BI GAGCTC 1 cut(s) 74
PspN4I GGNNCC 1 cut(s) 484
PsuI RGATCY 1 cut(s) 476
RsaI GTAC 3 cut(s) 37, 178, 484
RsaNI GTAC 3 cut(s) 36, 177, 483
SacI GAGCTC 1 cut(s) 74
SaqAI TTAA 2 cut(s) 30, 195
SatI GCNGC 3 cut(s) 252, 275, 454
Sau3AI GATC 2 cut(s) 401, 476
ScaI AGTACT 1 cut(s) 37
SduI GDGCHC 2 cut(s) 74, 361
SetI ASST 5 cut(s) 74, 261, 388, 440, 464
SfcI CTRYAG 1 cut(s) 493
SmlI CTYRAG 3 cut(s) 29, 186, 513
SmoI CTYRAG 3 cut(s) 29, 186, 513
SpeI ACTAGT 1 cut(s) 38
Sse9I AATT 2 cut(s) 76, 420
SseBI AGGCCT 1 cut(s) 412
SsiI CCGC 2 cut(s) 277, 442
SspMI CTAG 2 cut(s) 24, 39
SstI GAGCTC 1 cut(s) 74
StuI AGGCCT 1 cut(s) 412
StyI CCWWGG 1 cut(s) 353
TaaI ACNGT 2 cut(s) 247, 497
TaqI TCGA 1 cut(s) 378
TasI AATT 2 cut(s) 76, 420
TatI WGTACW 1 cut(s) 35
TfiI GAWTC 2 cut(s) 325, 349
Tru1I TTAA 2 cut(s) 30, 195
Tru9I TTAA 2 cut(s) 30, 195
TscAI CASTG 1 cut(s) 472
TseI GCWGC 3 cut(s) 251, 274, 453
TspDTI ATGAA 2 cut(s) 54, 114
TspRI CASTG 1 cut(s) 472
Vha464I CTTAAG 1 cut(s) 29
XapI RAATTY 1 cut(s) 76
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 2 cut(s) 24, 39
ZrmI AGTACT 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.