Rh3AG291700

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0005732
Physical Location & Seq
Reverse (-)
35288979 .. 35291469
2491 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG291700.1

Sequence Viewer

Length: 534 bp
ATGTTGAAGTCTGTGAAGAGTACTAGTCCTTCACACTCTGCTGTGAGTTCATGGAGCTCAAATTCTGGAGGAATGCCAAGAGATGAACTGAAAGAACGGGGATATGACAAAGACTACTACTATAAGGTTGATAGTCGTGTATATGCTTCTTATCAGAGCAAGTCTCCTACAAGAGAAAGTACCAAGCCTCAAGAAGTTAAACTTCACACTCAACAAAGGGCAACCAAAAGGGCAATGTTCAACTACATGGTTGCAGCAAAGTTGGGTATCTTGGAGCAGCGGAGAAAGCAAGAAGAGATGTTGCATAAGATGATAGAAGAGGAAGAGATTCGCTTACTGAGAAAGGAGATGATTCCAAGGGCTCAACTCATGCCGTTTTTCGATAGACCTTTTATTCCTCAAAGATCAAGCAGGCCTTTGACAATTCCTAAAGAACCAAGCTTCCGCATGATGAGCAGCAAGAGCTTCACTGCTTGTAGATCTGGTACCCCAATCTACAGTTTCCAACACACTACTCAAGCCCCCATCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

177

Amino Acids

20.65

Weight (kDa)

10.0

Isoelectric Point (pI)

75.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 67 - 141 3.8e-22 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 485
AccB1I GGYRCC 1 cut(s) 485
AciI CCGC 2 cut(s) 280, 445
AcsI RAATTY 1 cut(s) 61
AfaI GTAC 3 cut(s) 22, 181, 487
AgsI TTSAA 2 cut(s) 7, 241
AhlI ACTAGT 1 cut(s) 23
AloI GAACNNNNNNTCC 2 cut(s) 426, 458
AluBI AGCT 3 cut(s) 57, 441, 465
AluI AGCT 3 cut(s) 57, 441, 465
Alw21I GWGCWC 1 cut(s) 59
Alw26I GTCTC 1 cut(s) 168
AoxI GGCC 1 cut(s) 413
ApeKI GCWGC 3 cut(s) 254, 277, 456
ApoI RAATTY 1 cut(s) 61
Asp700I GAANNNNTTC 1 cut(s) 327
Asp718I GGTACC 1 cut(s) 485
BanI GGYRCC 1 cut(s) 485
BanII GRGCYC 2 cut(s) 59, 364
BarI GAAGNNNNNNTAC 2 cut(s) 13, 45
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 3 cut(s) 266, 289, 468
BccI CCATC 1 cut(s) 533
BceAI ACGGC 1 cut(s) 358
BcoDI GTCTC 1 cut(s) 168
BcuI ACTAGT 1 cut(s) 23
BfaI CTAG 1 cut(s) 24
BfmI CTRYAG 1 cut(s) 496
BglII AGATCT 1 cut(s) 479
BisI GCNGC 3 cut(s) 255, 278, 457
BlsI GCNGC 3 cut(s) 256, 279, 458
BmcAI AGTACT 1 cut(s) 22
BmiI GGNNCC 1 cut(s) 487
BplI GAGNNNNNCTC 2 cut(s) 148, 180
BpmI CTGGAG 1 cut(s) 87
BpuEI CTTGAG 2 cut(s) 174, 501
BsaJI CCNNGG 1 cut(s) 356
Bse3DI GCAATG 1 cut(s) 240
BseDI CCNNGG 1 cut(s) 356
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 1 cut(s) 329
BseXI GCAGC 3 cut(s) 266, 289, 468
BshFI GGCC 1 cut(s) 415
BshNI GGYRCC 1 cut(s) 485
BsiHKAI GWGCWC 1 cut(s) 59
BsmAI GTCTC 1 cut(s) 168
BsmI GAATGC 1 cut(s) 78
BsnI GGCC 1 cut(s) 415
Bsp1286I GDGCHC 2 cut(s) 59, 364
Bsp143I GATC 2 cut(s) 404, 479
BspACI CCGC 2 cut(s) 280, 445
BspANI GGCC 1 cut(s) 415
BspCNI CTCAG 1 cut(s) 330
BspLI GGNNCC 1 cut(s) 487
BspT107I GGYRCC 1 cut(s) 485
BsrDI GCAATG 1 cut(s) 240
BssECI CCNNGG 1 cut(s) 356
BssMI GATC 2 cut(s) 404, 479
BssT1I CCWWGG 1 cut(s) 356
Bst4CI ACNGT 1 cut(s) 500
Bst6I CTCTTC 4 cut(s) 11, 288, 312, 318
BstC8I GCNNGC 1 cut(s) 413
BstDEI CTNAG 1 cut(s) 338
BstKTI GATC 2 cut(s) 407, 482
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 2 cut(s) 404, 479
BstMWI GCNNNNNNNGC 3 cut(s) 286, 453, 462
BstSFI CTRYAG 1 cut(s) 496
BstV1I GCAGC 3 cut(s) 266, 289, 468
BstX2I RGATCY 1 cut(s) 479
BstYI RGATCY 1 cut(s) 479
BsuRI GGCC 1 cut(s) 415
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 1 cut(s) 468
Cac8I GCNNGC 1 cut(s) 413
Csp6I GTAC 3 cut(s) 21, 180, 486
CviAII CATG 4 cut(s) 51, 247, 370, 448
CviJI RGCY 7 cut(s) 57, 187, 362, 415, 441, 465, 521
CviKI_1 RGCY 7 cut(s) 57, 187, 362, 415, 441, 465, 521
CviQI GTAC 3 cut(s) 21, 180, 486
DdeI CTNAG 1 cut(s) 338
DpnI GATC 2 cut(s) 406, 481
DpnII GATC 2 cut(s) 404, 479
Eam1104I CTCTTC 4 cut(s) 11, 288, 312, 318
EarI CTCTTC 4 cut(s) 11, 288, 312, 318
Ecl136II GAGCTC 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 356
Eco147I AGGCCT 1 cut(s) 415
Eco24I GRGCYC 2 cut(s) 59, 364
Eco53kI GAGCTC 1 cut(s) 57
EcoICRI GAGCTC 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 356
EcoT38I GRGCYC 2 cut(s) 59, 364
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 4 cut(s) 54, 250, 373, 451
FaiI YATR 9 cut(s) 52, 105, 123, 142, 144, 248, 306, 371, 449
FalI AAGNNNNNCTT 4 cut(s) 186, 218, 400, 432
FatI CATG 4 cut(s) 50, 246, 369, 447
Fnu4HI GCNGC 3 cut(s) 255, 278, 457
FriOI GRGCYC 2 cut(s) 59, 364
Fsp4HI GCNGC 3 cut(s) 255, 278, 457
FspBI CTAG 1 cut(s) 24
GluI GCNGC 3 cut(s) 255, 278, 457
GsuI CTGGAG 1 cut(s) 87
HaeIII GGCC 1 cut(s) 415
Hin1II CATG 4 cut(s) 54, 250, 373, 451
HindIII AAGCTT 1 cut(s) 439
HinfI GANTC 2 cut(s) 328, 352
Hpy188I TCNGA 1 cut(s) 156
Hpy188III TCNNGA 2 cut(s) 66, 191
HpyAV CCTTC 1 cut(s) 39
HpyCH4III ACNGT 1 cut(s) 500
HpyCH4V TGCA 2 cut(s) 254, 304
HpyF10VI GCNNNNNNNGC 3 cut(s) 286, 453, 462
HpyF3I CTNAG 1 cut(s) 338
Hsp92II CATG 4 cut(s) 54, 250, 373, 451
KpnI GGTACC 1 cut(s) 489
Kzo9I GATC 2 cut(s) 404, 479
LmnI GCTCC 2 cut(s) 54, 274
LpnPI CCDG 3 cut(s) 51, 397, 468
Lsp1109I GCAGC 3 cut(s) 266, 289, 468
MaeI CTAG 1 cut(s) 24
MalI GATC 2 cut(s) 406, 481
MboI GATC 2 cut(s) 404, 479
MboII GAAGA 4 cut(s) 28, 305, 329, 335
MflI RGATCY 1 cut(s) 479
MhlI GDGCHC 2 cut(s) 59, 364
MluCI AATT 2 cut(s) 61, 423
MmeI TCCRAC 1 cut(s) 529
MnlI CCTC 4 cut(s) 62, 198, 313, 408
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 1 cut(s) 198
MspA1I CMGCKG 1 cut(s) 280
Mva1269I GAATGC 1 cut(s) 78
MwoI GCNNNNNNNGC 3 cut(s) 286, 453, 462
NdeII GATC 2 cut(s) 404, 479
NlaIII CATG 4 cut(s) 54, 250, 373, 451
NlaIV GGNNCC 1 cut(s) 487
PceI AGGCCT 1 cut(s) 415
PctI GAATGC 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 2 cut(s) 328, 352
PkrI GCNGC 3 cut(s) 256, 279, 458
Psp124BI GAGCTC 1 cut(s) 59
PspN4I GGNNCC 1 cut(s) 487
PsuI RGATCY 1 cut(s) 479
RsaI GTAC 3 cut(s) 22, 181, 487
RsaNI GTAC 3 cut(s) 21, 180, 486
SacI GAGCTC 1 cut(s) 59
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 3 cut(s) 255, 278, 457
Sau3AI GATC 2 cut(s) 404, 479
ScaI AGTACT 1 cut(s) 22
SduI GDGCHC 2 cut(s) 59, 364
SetI ASST 5 cut(s) 59, 129, 391, 443, 467
SfcI CTRYAG 1 cut(s) 496
SmlI CTYRAG 2 cut(s) 189, 516
SmoI CTYRAG 2 cut(s) 189, 516
SpeI ACTAGT 1 cut(s) 23
Sse9I AATT 2 cut(s) 61, 423
SseBI AGGCCT 1 cut(s) 415
SsiI CCGC 2 cut(s) 280, 445
SspMI CTAG 1 cut(s) 24
SstI GAGCTC 1 cut(s) 59
StuI AGGCCT 1 cut(s) 415
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 1 cut(s) 500
TaqI TCGA 1 cut(s) 381
TasI AATT 2 cut(s) 61, 423
TatI WGTACW 1 cut(s) 20
TfiI GAWTC 2 cut(s) 328, 352
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TscAI CASTG 1 cut(s) 475
TseI GCWGC 3 cut(s) 254, 277, 456
TspDTI ATGAA 2 cut(s) 39, 99
TspRI CASTG 1 cut(s) 475
XapI RAATTY 1 cut(s) 61
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 1 cut(s) 24
ZrmI AGTACT 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.