MD09G1276000.v1.1

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005732
Physical Location & Seq
Forward (+)
35344046 .. 35346584
2539 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1276000.v1.1.491

Sequence Viewer

Length: 480 bp
ATGGAGAAGACACACTCCAAATCTACTCTTAAGTTTGTGAAGAGTGCTTCACAGTTCACAACGGGTTTATGGAGCACAAATTCTGAAGGAATGTCTTGTCAGAAGTCTCCTACAATGGAAAATACCAAAGCTCAAGAAGTGAAACTTCACACTCAACAAAGAGCAACCAAACGAGCAATGTTCAACTACATGGTTGCGACCAAGCTGAGCATCTTGGAGCAGCAGAGAAAACAAGAAGAGATGTTGCAGAAGATGATAGAAGAGGAAGAGATTCGCTTGCTGAGAAAGGAGATGGTTCCAAGGGCTCAATTGATGCCATTTTTCGATAGACCTTTCATTCCGCAAAGATCGAGCAGGCCTTTAACAATTCCTAGAGAACCAAGTTTCCGAATGATGAGCAGCAAGGGCTTCAGCTGCAACTCTGGCAGTGAACTCTACAATTTCCAACCCACAACTCAAGCTATGAATCCCATCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

160

Amino Acids

18.51

Weight (kDa)

9.91

Isoelectric Point (pI)

68.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 48 - 122 1e-23 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 341
AcsI RAATTY 1 cut(s) 79
AcuI CTGAAG 2 cut(s) 105, 394
AflII CTTAAG 1 cut(s) 29
AgsI TTSAA 1 cut(s) 184
AloI GAACNNNNNNTCC 2 cut(s) 369, 401
AluBI AGCT 4 cut(s) 131, 205, 414, 461
AluI AGCT 4 cut(s) 131, 205, 414, 461
Alw21I GWGCWC 1 cut(s) 77
Alw26I GTCTC 1 cut(s) 111
AoxI GGCC 1 cut(s) 356
ApeKI GCWGC 3 cut(s) 220, 399, 414
ApoI RAATTY 1 cut(s) 79
Asp700I GAANNNNTTC 1 cut(s) 270
BanII GRGCYC 1 cut(s) 307
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 77
BbvI GCAGC 3 cut(s) 232, 401, 411
BccI CCATC 2 cut(s) 286, 479
BcoDI GTCTC 1 cut(s) 111
BfaI CTAG 1 cut(s) 372
BfrI CTTAAG 1 cut(s) 29
BisI GCNGC 3 cut(s) 221, 400, 415
BlpI GCTNAGC 1 cut(s) 206
BlsI GCNGC 3 cut(s) 222, 401, 416
BmiI GGNNCC 1 cut(s) 297
BmsI GCATC 2 cut(s) 219, 303
BpiI GAAGAC 1 cut(s) 14
Bpu1102I GCTNAGC 1 cut(s) 206
BpuEI CTTGAG 2 cut(s) 117, 441
BsaJI CCNNGG 1 cut(s) 299
Bse3DI GCAATG 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 299
BseMI GCAATG 1 cut(s) 183
BseMII CTCAG 2 cut(s) 197, 272
BseXI GCAGC 3 cut(s) 232, 401, 411
BshFI GGCC 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 111
BsnI GGCC 1 cut(s) 358
Bsp1286I GDGCHC 2 cut(s) 77, 307
Bsp143I GATC 1 cut(s) 347
Bsp1720I GCTNAGC 1 cut(s) 206
BspACI CCGC 1 cut(s) 341
BspANI GGCC 1 cut(s) 358
BspCNI CTCAG 2 cut(s) 198, 273
BspLI GGNNCC 1 cut(s) 297
BspTI CTTAAG 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 183
BssECI CCNNGG 1 cut(s) 299
BssMI GATC 1 cut(s) 347
BssT1I CCWWGG 1 cut(s) 299
Bst4CI ACNGT 1 cut(s) 54
Bst6I CTCTTC 4 cut(s) 35, 231, 255, 261
BstAFI CTTAAG 1 cut(s) 29
BstC8I GCNNGC 2 cut(s) 278, 356
BstDEI CTNAG 2 cut(s) 206, 281
BstKTI GATC 1 cut(s) 350
BstMAI GTCTC 1 cut(s) 111
BstMBI GATC 1 cut(s) 347
BstMWI GCNNNNNNNGC 3 cut(s) 405, 414, 423
BstV1I GCAGC 3 cut(s) 232, 401, 411
BstV2I GAAGAC 1 cut(s) 14
BsuRI GGCC 1 cut(s) 358
BtsI GCAGTG 1 cut(s) 433
BtsIMutI CAGTG 1 cut(s) 433
Cac8I GCNNGC 2 cut(s) 278, 356
CviAII CATG 1 cut(s) 190
CviJI RGCY 7 cut(s) 131, 205, 305, 358, 408, 414, 461
CviKI_1 RGCY 7 cut(s) 131, 205, 305, 358, 408, 414, 461
DdeI CTNAG 2 cut(s) 206, 281
DpnI GATC 1 cut(s) 349
DpnII GATC 1 cut(s) 347
Eam1104I CTCTTC 4 cut(s) 35, 231, 255, 261
EarI CTCTTC 4 cut(s) 35, 231, 255, 261
Eco130I CCWWGG 1 cut(s) 299
Eco147I AGGCCT 1 cut(s) 358
Eco24I GRGCYC 1 cut(s) 307
Eco57I CTGAAG 2 cut(s) 105, 394
EcoT14I CCWWGG 1 cut(s) 299
EcoT38I GRGCYC 1 cut(s) 307
ErhI CCWWGG 1 cut(s) 299
FaeI CATG 1 cut(s) 193
FaiI YATR 3 cut(s) 70, 191, 464
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FatI CATG 1 cut(s) 189
Fnu4HI GCNGC 3 cut(s) 221, 400, 415
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 3 cut(s) 221, 400, 415
FspBI CTAG 1 cut(s) 372
GluI GCNGC 3 cut(s) 221, 400, 415
HaeIII GGCC 1 cut(s) 358
Hin1II CATG 1 cut(s) 193
HinfI GANTC 2 cut(s) 271, 466
Hpy166II GTNNAC 2 cut(s) 57, 431
Hpy188I TCNGA 3 cut(s) 85, 102, 389
Hpy188III TCNNGA 1 cut(s) 134
Hpy8I GTNNAC 2 cut(s) 57, 431
HpyAV CCTTC 1 cut(s) 80
HpyCH4III ACNGT 1 cut(s) 54
HpyCH4V TGCA 2 cut(s) 247, 417
HpyF10VI GCNNNNNNNGC 3 cut(s) 405, 414, 423
HpyF3I CTNAG 2 cut(s) 206, 281
Hsp92II CATG 1 cut(s) 193
Kzo9I GATC 1 cut(s) 347
LmnI GCTCC 2 cut(s) 72, 217
LpnPI CCDG 2 cut(s) 340, 408
Lsp1109I GCAGC 3 cut(s) 232, 401, 411
LweI GCATC 2 cut(s) 219, 303
MaeI CTAG 1 cut(s) 372
MalI GATC 1 cut(s) 349
MboI GATC 1 cut(s) 347
MboII GAAGA 6 cut(s) 19, 52, 248, 262, 272, 278
MfeI CAATTG 1 cut(s) 308
MhlI GDGCHC 2 cut(s) 77, 307
MluCI AATT 4 cut(s) 79, 308, 366, 439
MmeI TCCRAC 1 cut(s) 469
MnlI CCTC 1 cut(s) 256
MroXI GAANNNNTTC 1 cut(s) 270
MseI TTAA 2 cut(s) 30, 362
MspA1I CMGCKG 1 cut(s) 414
MspCI CTTAAG 1 cut(s) 29
MunI CAATTG 1 cut(s) 308
MwoI GCNNNNNNNGC 3 cut(s) 405, 414, 423
NdeII GATC 1 cut(s) 347
NlaIII CATG 1 cut(s) 193
NlaIV GGNNCC 1 cut(s) 297
PceI AGGCCT 1 cut(s) 358
PdmI GAANNNNTTC 1 cut(s) 270
PfeI GAWTC 2 cut(s) 271, 466
PkrI GCNGC 3 cut(s) 222, 401, 416
PspN4I GGNNCC 1 cut(s) 297
PvuII CAGCTG 1 cut(s) 414
SaqAI TTAA 2 cut(s) 30, 362
SatI GCNGC 3 cut(s) 221, 400, 415
Sau3AI GATC 1 cut(s) 347
SduI GDGCHC 2 cut(s) 77, 307
SetI ASST 5 cut(s) 133, 207, 334, 416, 463
SfaNI GCATC 2 cut(s) 219, 303
SmlI CTYRAG 3 cut(s) 29, 132, 456
SmoI CTYRAG 3 cut(s) 29, 132, 456
Sse9I AATT 4 cut(s) 79, 308, 366, 439
SseBI AGGCCT 1 cut(s) 358
SsiI CCGC 1 cut(s) 341
SspMI CTAG 1 cut(s) 372
StuI AGGCCT 1 cut(s) 358
StyI CCWWGG 1 cut(s) 299
TaaI ACNGT 1 cut(s) 54
TaqI TCGA 2 cut(s) 324, 350
TasI AATT 4 cut(s) 79, 308, 366, 439
TfiI GAWTC 2 cut(s) 271, 466
Tru1I TTAA 2 cut(s) 30, 362
Tru9I TTAA 2 cut(s) 30, 362
TscAI CASTG 1 cut(s) 433
TseI GCWGC 3 cut(s) 220, 399, 414
TspDTI ATGAA 2 cut(s) 325, 479
TspRI CASTG 1 cut(s) 433
Vha464I CTTAAG 1 cut(s) 29
XapI RAATTY 1 cut(s) 79
XmnI GAANNNNTTC 1 cut(s) 270
XspI CTAG 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.