MD17G1270100.v1.1

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005732
Physical Location & Seq
Forward (+)
33107415 .. 33109270
1856 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1270100.v1.1.491

Sequence Viewer

Length: 366 bp
ATGGAAAATACCAAAGCTCAAGAAGTTAAACTTCACACTCAAGAAAGAGCAACCAAACGAGCAATGTTCAACTACATGGTAGCAACCAAGCTGAGTATCTTGGAGCATCAGAGAAAACAAGAAGAGAGGTTACAGAAGATGATAGAAGAGGAAGAGATTCGCTTACTGAGAAAGGAGATGGTTCCAAGGGCTCAGTTGATGCCTTTTTTCGATAGACCTTTCATTCCACGAAGATCAAGCAGGCCCTTGACGATTCCTAGGGAACCAAGTTTCCGCATGTTGAGCAGCAAGTATTTAAGCTGCAACCCTGGCAGTCAACTCTACAATTTCCAACCGACAACTCGAGCTACGAATCCTATCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

122

Amino Acids

14.47

Weight (kDa)

10.27

Isoelectric Point (pI)

71.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 10 - 84 5e-24 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 274
AgsI TTSAA 1 cut(s) 70
AjnI CCWGG 1 cut(s) 307
AloI GAACNNNNNNTCC 2 cut(s) 255, 287
AluBI AGCT 4 cut(s) 17, 91, 300, 347
AluI AGCT 4 cut(s) 17, 91, 300, 347
Ama87I CYCGRG 1 cut(s) 342
AoxI GGCC 1 cut(s) 242
ApeKI GCWGC 2 cut(s) 285, 300
Asp700I GAANNNNTTC 1 cut(s) 156
AspA2I CCTAGG 1 cut(s) 257
AspS9I GGNCC 1 cut(s) 243
AvaI CYCGRG 1 cut(s) 342
AvrII CCTAGG 1 cut(s) 257
BanII GRGCYC 1 cut(s) 193
BarI GAAGNNNNNNTAC 2 cut(s) 114, 146
BbvI GCAGC 2 cut(s) 287, 297
BccI CCATC 1 cut(s) 172
BciT130I CCWGG 1 cut(s) 309
BfaI CTAG 1 cut(s) 258
BisI GCNGC 2 cut(s) 286, 301
BlnI CCTAGG 1 cut(s) 257
BlsI GCNGC 2 cut(s) 287, 302
Bme1390I CCNGG 1 cut(s) 309
BmeT110I CYCGRG 1 cut(s) 342
BmgT120I GGNCC 1 cut(s) 243
BmiI GGNNCC 2 cut(s) 183, 264
BmrFI CCNGG 1 cut(s) 309
BmsI GCATC 2 cut(s) 115, 189
BpuEI CTTGAG 1 cut(s) 24
BsaJI CCNNGG 3 cut(s) 185, 257, 307
Bse3DI GCAATG 1 cut(s) 69
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 3 cut(s) 185, 257, 307
BseMI GCAATG 1 cut(s) 69
BseMII CTCAG 3 cut(s) 83, 158, 206
BseXI GCAGC 2 cut(s) 287, 297
BshFI GGCC 1 cut(s) 244
BsiHKCI CYCGRG 1 cut(s) 342
BsnI GGCC 1 cut(s) 244
BsoBI CYCGRG 1 cut(s) 342
Bsp1286I GDGCHC 1 cut(s) 193
Bsp143I GATC 1 cut(s) 233
BspACI CCGC 1 cut(s) 274
BspANI GGCC 1 cut(s) 244
BspCNI CTCAG 3 cut(s) 84, 159, 205
BspLI GGNNCC 2 cut(s) 183, 264
BsrDI GCAATG 1 cut(s) 69
BssECI CCNNGG 3 cut(s) 185, 257, 307
BssMI GATC 1 cut(s) 233
BssT1I CCWWGG 2 cut(s) 185, 257
Bst2UI CCWGG 1 cut(s) 309
Bst6I CTCTTC 3 cut(s) 117, 141, 147
BstC8I GCNNGC 1 cut(s) 242
BstDEI CTNAG 3 cut(s) 92, 167, 192
BstKTI GATC 1 cut(s) 236
BstMBI GATC 1 cut(s) 233
BstMWI GCNNNNNNNGC 2 cut(s) 282, 309
BstNI CCWGG 1 cut(s) 309
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 307
BstV1I GCAGC 2 cut(s) 287, 297
BsuRI GGCC 1 cut(s) 244
Cac8I GCNNGC 1 cut(s) 242
Cfr13I GGNCC 1 cut(s) 243
CviAII CATG 2 cut(s) 76, 277
CviJI RGCY 6 cut(s) 17, 91, 191, 244, 300, 347
CviKI_1 RGCY 6 cut(s) 17, 91, 191, 244, 300, 347
DdeI CTNAG 3 cut(s) 92, 167, 192
DpnI GATC 1 cut(s) 235
DpnII GATC 1 cut(s) 233
Eam1104I CTCTTC 3 cut(s) 117, 141, 147
EarI CTCTTC 3 cut(s) 117, 141, 147
Eco130I CCWWGG 2 cut(s) 185, 257
Eco24I GRGCYC 1 cut(s) 193
Eco88I CYCGRG 1 cut(s) 342
EcoO109I RGGNCCY 1 cut(s) 243
EcoRII CCWGG 1 cut(s) 307
EcoT14I CCWWGG 2 cut(s) 185, 257
EcoT38I GRGCYC 1 cut(s) 193
ErhI CCWWGG 2 cut(s) 185, 257
FaeI CATG 2 cut(s) 79, 280
FaiI YATR 2 cut(s) 77, 278
FalI AAGNNNNNCTT 2 cut(s) 15, 47
FatI CATG 2 cut(s) 75, 276
Fnu4HI GCNGC 2 cut(s) 286, 301
FriOI GRGCYC 1 cut(s) 193
Fsp4HI GCNGC 2 cut(s) 286, 301
FspBI CTAG 1 cut(s) 258
GluI GCNGC 2 cut(s) 286, 301
HaeIII GGCC 1 cut(s) 244
Hin1II CATG 2 cut(s) 79, 280
HincII GTYRAC 1 cut(s) 317
HindII GTYRAC 1 cut(s) 317
HinfI GANTC 3 cut(s) 157, 253, 352
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 1 cut(s) 111
Hpy188III TCNNGA 2 cut(s) 20, 41
Hpy8I GTNNAC 1 cut(s) 317
HpyCH4V TGCA 1 cut(s) 303
HpyF10VI GCNNNNNNNGC 2 cut(s) 282, 309
HpyF3I CTNAG 3 cut(s) 92, 167, 192
Hsp92II CATG 2 cut(s) 79, 280
Kzo9I GATC 1 cut(s) 233
LmnI GCTCC 1 cut(s) 103
LpnPI CCDG 3 cut(s) 226, 294, 321
Lsp1109I GCAGC 2 cut(s) 287, 297
LweI GCATC 2 cut(s) 115, 189
MaeI CTAG 1 cut(s) 258
MaeIII GTNAC 1 cut(s) 129
MalI GATC 1 cut(s) 235
MboI GATC 1 cut(s) 233
MboII GAAGA 5 cut(s) 134, 148, 158, 164, 243
MhlI GDGCHC 1 cut(s) 193
MluCI AATT 1 cut(s) 325
MmeI TCCRAC 1 cut(s) 355
MnlI CCTC 2 cut(s) 120, 142
MroXI GAANNNNTTC 1 cut(s) 156
MseI TTAA 2 cut(s) 27, 296
MspR9I CCNGG 1 cut(s) 309
MvaI CCWGG 1 cut(s) 309
MwoI GCNNNNNNNGC 2 cut(s) 282, 309
NdeII GATC 1 cut(s) 233
NlaIII CATG 2 cut(s) 79, 280
NlaIV GGNNCC 2 cut(s) 183, 264
NspI RCATGY 1 cut(s) 280
PaeR7I CTCGAG 1 cut(s) 342
PdmI GAANNNNTTC 1 cut(s) 156
PfeI GAWTC 3 cut(s) 157, 253, 352
PkrI GCNGC 2 cut(s) 287, 302
Psp6I CCWGG 1 cut(s) 307
PspGI CCWGG 1 cut(s) 307
PspN4I GGNNCC 2 cut(s) 183, 264
PspPI GGNCC 1 cut(s) 243
PspXI VCTCGAGB 1 cut(s) 342
SaqAI TTAA 2 cut(s) 27, 296
SatI GCNGC 2 cut(s) 286, 301
Sau3AI GATC 1 cut(s) 233
Sau96I GGNCC 1 cut(s) 243
ScrFI CCNGG 1 cut(s) 309
SduI GDGCHC 1 cut(s) 193
SetI ASST 6 cut(s) 19, 93, 131, 220, 302, 349
SfaNI GCATC 2 cut(s) 115, 189
Sfr274I CTCGAG 1 cut(s) 342
SlaI CTCGAG 1 cut(s) 342
SmlI CTYRAG 3 cut(s) 18, 39, 342
SmoI CTYRAG 3 cut(s) 18, 39, 342
Sse9I AATT 1 cut(s) 325
SsiI CCGC 1 cut(s) 274
SspMI CTAG 1 cut(s) 258
StyD4I CCNGG 1 cut(s) 307
StyI CCWWGG 2 cut(s) 185, 257
TaqI TCGA 2 cut(s) 210, 343
TasI AATT 1 cut(s) 325
TfiI GAWTC 3 cut(s) 157, 253, 352
Tru1I TTAA 2 cut(s) 27, 296
Tru9I TTAA 2 cut(s) 27, 296
TseI GCWGC 2 cut(s) 285, 300
TspDTI ATGAA 1 cut(s) 211
XceI RCATGY 1 cut(s) 280
XhoI CTCGAG 1 cut(s) 342
XmaJI CCTAGG 1 cut(s) 257
XmnI GAANNNNTTC 1 cut(s) 156
XspI CTAG 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.