FvH4_1g30340

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005732
Physical Location & Seq
Reverse (-)
24053628 .. 24056112
2485 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g30340.t1

Sequence Viewer

Length: 462 bp
ATGGACAAGGCATACACCACGGCTAATCTTAAGACAAGGGACGAACTCAAAGAACGGGGATATGACAAAGACTACTACTACTACTCCAAGGCTTCTTATCAGAGCAAGTCTCCTACAAGAGAAAATACCAAGCCTCAAGAAGTCAAACTTCACACTCAAGAAAGGGCAACCAAACGGGCAATGTTCAACTACATGGTTGCAGCAAAGCTGAGTATCTTGGAGCAGCGGAGAAAGCAAGAGGAGATGTTGCATAAGATGATAGAAGAGGAAGAGATTCGCTTACTGAGAAAGGAGATGATTCCGAGGGCTCAGCTCATGCCCTTTTTCGATAGACCGTTCGTTCCTCAAAGATCCAGCAGGCCTTTGACAATTCCTAAAGAACCAAGCTTCCGCATGATGAGCAGCAAGTGCTGCAATTGTCTATCCGGTACCCCAATCTACAATTTCCAACAGACTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

154

Amino Acids

18.29

Weight (kDa)

9.58

Isoelectric Point (pI)

70.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 49 - 123 2.1e-22 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 428
AccB1I GGYRCC 1 cut(s) 428
AciI CCGC 2 cut(s) 226, 391
AclWI GGATC 1 cut(s) 345
AfaI GTAC 1 cut(s) 430
AflII CTTAAG 1 cut(s) 29
AgsI TTSAA 1 cut(s) 187
AloI GAACNNNNNNTCC 2 cut(s) 372, 404
AluBI AGCT 3 cut(s) 208, 313, 387
AluI AGCT 3 cut(s) 208, 313, 387
Alw26I GTCTC 1 cut(s) 114
AlwI GGATC 1 cut(s) 345
AoxI GGCC 1 cut(s) 359
ApeKI GCWGC 4 cut(s) 200, 223, 402, 411
Asp700I GAANNNNTTC 1 cut(s) 273
Asp718I GGTACC 1 cut(s) 428
BanI GGYRCC 1 cut(s) 428
BanII GRGCYC 1 cut(s) 310
BbvI GCAGC 4 cut(s) 212, 235, 398, 414
BceAI ACGGC 1 cut(s) 36
BcoDI GTCTC 1 cut(s) 114
BfaI CTAG 1 cut(s) 460
BfrI CTTAAG 1 cut(s) 29
BisI GCNGC 4 cut(s) 201, 224, 403, 412
BlpI GCTNAGC 1 cut(s) 309
BlsI GCNGC 4 cut(s) 202, 225, 404, 413
BmiI GGNNCC 1 cut(s) 430
BplI GAGNNNNNCTC 2 cut(s) 94, 126
Bpu1102I GCTNAGC 1 cut(s) 309
BpuEI CTTGAG 2 cut(s) 120, 141
BsaJI CCNNGG 3 cut(s) 18, 87, 302
BsaWI WCCGGW 1 cut(s) 425
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bse3DI GCAATG 1 cut(s) 186
BseDI CCNNGG 3 cut(s) 18, 87, 302
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 3 cut(s) 200, 275, 323
BseRI GAGGAG 1 cut(s) 254
BseXI GCAGC 4 cut(s) 212, 235, 398, 414
BshFI GGCC 1 cut(s) 361
BshNI GGYRCC 1 cut(s) 428
BsiSI CCGG 1 cut(s) 426
BslFI GGGAC 1 cut(s) 53
BsmAI GTCTC 1 cut(s) 114
BsmFI GGGAC 1 cut(s) 53
BsnI GGCC 1 cut(s) 361
Bsp1286I GDGCHC 1 cut(s) 310
Bsp143I GATC 1 cut(s) 350
Bsp1720I GCTNAGC 1 cut(s) 309
BspACI CCGC 2 cut(s) 226, 391
BspANI GGCC 1 cut(s) 361
BspCNI CTCAG 3 cut(s) 201, 276, 322
BspLI GGNNCC 1 cut(s) 430
BspPI GGATC 1 cut(s) 345
BspT107I GGYRCC 1 cut(s) 428
BspTI CTTAAG 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 186
BssECI CCNNGG 3 cut(s) 18, 87, 302
BssMI GATC 1 cut(s) 350
BssT1I CCWWGG 1 cut(s) 87
Bst4CI ACNGT 1 cut(s) 336
Bst6I CTCTTC 2 cut(s) 258, 264
BstAFI CTTAAG 1 cut(s) 29
BstAPI GCANNNNNTGC 2 cut(s) 408, 411
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 3 cut(s) 209, 284, 309
BstDSI CCRYGG 1 cut(s) 18
BstKTI GATC 1 cut(s) 353
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 1 cut(s) 350
BstMWI GCNNNNNNNGC 4 cut(s) 232, 399, 408, 411
BstV1I GCAGC 4 cut(s) 212, 235, 398, 414
BstX2I RGATCY 1 cut(s) 350
BstYI RGATCY 1 cut(s) 350
BsuRI GGCC 1 cut(s) 361
BtgI CCRYGG 1 cut(s) 18
Cac8I GCNNGC 1 cut(s) 359
Csp6I GTAC 1 cut(s) 429
CviAII CATG 3 cut(s) 193, 316, 394
CviJI RGCY 8 cut(s) 23, 92, 133, 208, 308, 313, 361, 387
CviKI_1 RGCY 8 cut(s) 23, 92, 133, 208, 308, 313, 361, 387
CviQI GTAC 1 cut(s) 429
DdeI CTNAG 3 cut(s) 209, 284, 309
DpnI GATC 1 cut(s) 352
DpnII GATC 1 cut(s) 350
Eam1104I CTCTTC 2 cut(s) 258, 264
EarI CTCTTC 2 cut(s) 258, 264
Eco130I CCWWGG 1 cut(s) 87
Eco147I AGGCCT 1 cut(s) 361
Eco24I GRGCYC 1 cut(s) 310
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 3 cut(s) 196, 319, 397
FaiI YATR 6 cut(s) 13, 63, 194, 252, 317, 395
FalI AAGNNNNNCTT 2 cut(s) 132, 164
FaqI GGGAC 1 cut(s) 53
FatI CATG 3 cut(s) 192, 315, 393
Fnu4HI GCNGC 4 cut(s) 201, 224, 403, 412
FriOI GRGCYC 1 cut(s) 310
Fsp4HI GCNGC 4 cut(s) 201, 224, 403, 412
FspBI CTAG 1 cut(s) 460
GluI GCNGC 4 cut(s) 201, 224, 403, 412
HaeIII GGCC 1 cut(s) 361
HapII CCGG 1 cut(s) 426
Hin1II CATG 3 cut(s) 196, 319, 397
HindIII AAGCTT 1 cut(s) 385
HinfI GANTC 2 cut(s) 274, 298
HpaII CCGG 1 cut(s) 426
Hpy188I TCNGA 2 cut(s) 102, 303
Hpy188III TCNNGA 2 cut(s) 137, 158
HpyCH4III ACNGT 1 cut(s) 336
HpyCH4V TGCA 3 cut(s) 200, 250, 414
HpyF10VI GCNNNNNNNGC 4 cut(s) 232, 399, 408, 411
HpyF3I CTNAG 3 cut(s) 209, 284, 309
Hsp92II CATG 3 cut(s) 196, 319, 397
KpnI GGTACC 1 cut(s) 432
Kzo9I GATC 1 cut(s) 350
LmnI GCTCC 1 cut(s) 220
LpnPI CCDG 3 cut(s) 343, 367, 439
Lsp1109I GCAGC 4 cut(s) 212, 235, 398, 414
MaeI CTAG 1 cut(s) 460
MalI GATC 1 cut(s) 352
MboI GATC 1 cut(s) 350
MboII GAAGA 2 cut(s) 275, 281
MfeI CAATTG 1 cut(s) 415
MflI RGATCY 1 cut(s) 350
MhlI GDGCHC 1 cut(s) 310
MluCI AATT 3 cut(s) 369, 415, 442
MnlI CCTC 5 cut(s) 144, 232, 259, 297, 354
MroXI GAANNNNTTC 1 cut(s) 273
MseI TTAA 1 cut(s) 30
MspA1I CMGCKG 1 cut(s) 226
MspCI CTTAAG 1 cut(s) 29
MspI CCGG 1 cut(s) 426
MunI CAATTG 1 cut(s) 415
MwoI GCNNNNNNNGC 4 cut(s) 232, 399, 408, 411
NdeII GATC 1 cut(s) 350
NlaIII CATG 3 cut(s) 196, 319, 397
NlaIV GGNNCC 1 cut(s) 430
PceI AGGCCT 1 cut(s) 361
PdmI GAANNNNTTC 1 cut(s) 273
PfeI GAWTC 2 cut(s) 274, 298
PkrI GCNGC 4 cut(s) 202, 225, 404, 413
PspN4I GGNNCC 1 cut(s) 430
PsuI RGATCY 1 cut(s) 350
RsaI GTAC 1 cut(s) 430
RsaNI GTAC 1 cut(s) 429
SaqAI TTAA 1 cut(s) 30
SatI GCNGC 4 cut(s) 201, 224, 403, 412
Sau3AI GATC 1 cut(s) 350
SduI GDGCHC 1 cut(s) 310
SetI ASST 4 cut(s) 210, 315, 389, 461
SmlI CTYRAG 3 cut(s) 29, 135, 156
SmoI CTYRAG 3 cut(s) 29, 135, 156
Sse9I AATT 3 cut(s) 369, 415, 442
SseBI AGGCCT 1 cut(s) 361
SsiI CCGC 2 cut(s) 226, 391
SspMI CTAG 1 cut(s) 460
StuI AGGCCT 1 cut(s) 361
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 1 cut(s) 336
TaqI TCGA 1 cut(s) 327
TasI AATT 3 cut(s) 369, 415, 442
TfiI GAWTC 2 cut(s) 274, 298
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TseI GCWGC 4 cut(s) 200, 223, 402, 411
Vha464I CTTAAG 1 cut(s) 29
XmnI GAANNNNTTC 1 cut(s) 273
XspI CTAG 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.