Rh3DG325000

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0005732
Physical Location & Seq
Reverse (-)
35584724 .. 35586845
2122 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG325000.1

Sequence Viewer

Length: 432 bp
ATGGAGAAGGCACTCACCAAGTCTAGTCTTAAGTCTGTGAAGAGTACTAGTCCTTCACACTCTGCTGTGAGTTCATGGAGCTCAAATTCTGGAGGAATGCCAAGAGATGAACTGAAAGAACGGGGATATGACAAAGACTACTACTATAAGGTTGATAGTCGTGTATATGCTTCTTATCAGAGCAAGTCTCCTACAAGAGAAAGTACCAAGCCTCAAGAAGTTAAACTTCACACTCAACAAAGGGCAACCAAAAGGGCAATGTTCAACTACATGGTTGCAGCAAAGTTGGGTATCTTGGAGCAGCGGAGAAAGCAAGAAGAGATGTTGCATAAGATGATAGAAGAGGAAGAGGTTCGCTTACTGAGAAAGGAGATGATTCCAAGGGCTCAACTCATGCCGTTTTTCGATAGACCTTTTATTCCTCAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

143

Amino Acids

16.8

Weight (kDa)

9.83

Isoelectric Point (pI)

72.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 75 - 143 4.7e-19 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 304
AcsI RAATTY 1 cut(s) 85
AfaI GTAC 2 cut(s) 46, 205
AflII CTTAAG 1 cut(s) 29
AgsI TTSAA 1 cut(s) 265
AhlI ACTAGT 1 cut(s) 47
AluBI AGCT 1 cut(s) 81
AluI AGCT 1 cut(s) 81
Alw21I GWGCWC 1 cut(s) 83
Alw26I GTCTC 1 cut(s) 192
ApeKI GCWGC 2 cut(s) 278, 301
ApoI RAATTY 1 cut(s) 85
Asp700I GAANNNNTTC 1 cut(s) 351
AsuHPI GGTGA 1 cut(s) 7
BanII GRGCYC 2 cut(s) 83, 388
BarI GAAGNNNNNNTAC 2 cut(s) 37, 69
Bbv12I GWGCWC 1 cut(s) 83
BbvI GCAGC 2 cut(s) 290, 313
BceAI ACGGC 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 192
BcuI ACTAGT 1 cut(s) 47
BfaI CTAG 2 cut(s) 24, 48
BfrI CTTAAG 1 cut(s) 29
BisI GCNGC 2 cut(s) 279, 302
BlsI GCNGC 2 cut(s) 280, 303
BmcAI AGTACT 1 cut(s) 46
BplI GAGNNNNNCTC 2 cut(s) 172, 204
BpmI CTGGAG 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 198
BsaJI CCNNGG 1 cut(s) 380
Bse3DI GCAATG 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 380
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 1 cut(s) 353
BseXI GCAGC 2 cut(s) 290, 313
BsiHKAI GWGCWC 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 192
BsmI GAATGC 1 cut(s) 102
Bsp1286I GDGCHC 2 cut(s) 83, 388
BspACI CCGC 1 cut(s) 304
BspCNI CTCAG 1 cut(s) 354
BspTI CTTAAG 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 264
BssECI CCNNGG 1 cut(s) 380
BssT1I CCWWGG 1 cut(s) 380
Bst6I CTCTTC 4 cut(s) 35, 312, 336, 342
BstAFI CTTAAG 1 cut(s) 29
BstDEI CTNAG 1 cut(s) 362
BstMAI GTCTC 1 cut(s) 192
BstMWI GCNNNNNNNGC 1 cut(s) 310
BstV1I GCAGC 2 cut(s) 290, 313
Csp6I GTAC 2 cut(s) 45, 204
CviAII CATG 3 cut(s) 75, 271, 394
CviJI RGCY 3 cut(s) 81, 211, 386
CviKI_1 RGCY 3 cut(s) 81, 211, 386
CviQI GTAC 2 cut(s) 45, 204
DdeI CTNAG 1 cut(s) 362
Eam1104I CTCTTC 4 cut(s) 35, 312, 336, 342
EarI CTCTTC 4 cut(s) 35, 312, 336, 342
Ecl136II GAGCTC 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 380
Eco24I GRGCYC 2 cut(s) 83, 388
Eco53kI GAGCTC 1 cut(s) 81
EcoICRI GAGCTC 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 380
EcoT38I GRGCYC 2 cut(s) 83, 388
ErhI CCWWGG 1 cut(s) 380
FaeI CATG 3 cut(s) 78, 274, 397
FaiI YATR 8 cut(s) 76, 129, 147, 166, 168, 272, 330, 395
FalI AAGNNNNNCTT 2 cut(s) 210, 242
FatI CATG 3 cut(s) 74, 270, 393
Fnu4HI GCNGC 2 cut(s) 279, 302
FriOI GRGCYC 2 cut(s) 83, 388
Fsp4HI GCNGC 2 cut(s) 279, 302
FspBI CTAG 2 cut(s) 24, 48
GluI GCNGC 2 cut(s) 279, 302
GsuI CTGGAG 1 cut(s) 111
Hin1II CATG 3 cut(s) 78, 274, 397
HinfI GANTC 1 cut(s) 376
HphI GGTGA 1 cut(s) 7
Hpy188I TCNGA 1 cut(s) 180
Hpy188III TCNNGA 2 cut(s) 90, 215
HpyAV CCTTC 1 cut(s) 63
HpyCH4V TGCA 2 cut(s) 278, 328
HpyF10VI GCNNNNNNNGC 1 cut(s) 310
HpyF3I CTNAG 1 cut(s) 362
Hsp92II CATG 3 cut(s) 78, 274, 397
LmnI GCTCC 2 cut(s) 78, 298
LpnPI CCDG 1 cut(s) 75
Lsp1109I GCAGC 2 cut(s) 290, 313
MaeI CTAG 2 cut(s) 24, 48
MboII GAAGA 4 cut(s) 52, 329, 353, 359
MhlI GDGCHC 2 cut(s) 83, 388
MluCI AATT 1 cut(s) 85
MnlI CCTC 5 cut(s) 86, 222, 337, 343, 432
MroXI GAANNNNTTC 1 cut(s) 351
MseI TTAA 2 cut(s) 30, 222
MspA1I CMGCKG 1 cut(s) 304
MspCI CTTAAG 1 cut(s) 29
Mva1269I GAATGC 1 cut(s) 102
MwoI GCNNNNNNNGC 1 cut(s) 310
NlaIII CATG 3 cut(s) 78, 274, 397
PctI GAATGC 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 351
PfeI GAWTC 1 cut(s) 376
PkrI GCNGC 2 cut(s) 280, 303
Psp124BI GAGCTC 1 cut(s) 83
RsaI GTAC 2 cut(s) 46, 205
RsaNI GTAC 2 cut(s) 45, 204
SacI GAGCTC 1 cut(s) 83
SaqAI TTAA 2 cut(s) 30, 222
SatI GCNGC 2 cut(s) 279, 302
ScaI AGTACT 1 cut(s) 46
SduI GDGCHC 2 cut(s) 83, 388
SetI ASST 5 cut(s) 83, 153, 354, 415, 431
SmlI CTYRAG 2 cut(s) 29, 213
SmoI CTYRAG 2 cut(s) 29, 213
SpeI ACTAGT 1 cut(s) 47
Sse9I AATT 1 cut(s) 85
SsiI CCGC 1 cut(s) 304
SspMI CTAG 2 cut(s) 24, 48
SstI GAGCTC 1 cut(s) 83
StyI CCWWGG 1 cut(s) 380
TaqI TCGA 1 cut(s) 405
TasI AATT 1 cut(s) 85
TatI WGTACW 1 cut(s) 44
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 2 cut(s) 30, 222
Tru9I TTAA 2 cut(s) 30, 222
TseI GCWGC 2 cut(s) 278, 301
TspDTI ATGAA 2 cut(s) 63, 123
Vha464I CTTAAG 1 cut(s) 29
XapI RAATTY 1 cut(s) 85
XmnI GAANNNNTTC 1 cut(s) 351
XspI CTAG 2 cut(s) 24, 48
ZrmI AGTACT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.