RchiOBHm_Chr3g0490251

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0005732
Physical Location & Seq
Reverse (-)
36936841 .. 36937760
920 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45343

Sequence Viewer

Length: 384 bp
ATGTACGCTTCTTATCAGAGCAAGTCTCCTACAAGAGAAAGTACCAAGCCTCAAGAAGTTAAACTTCACACTCAACAAAGGGCAACCAAAAGGGCAATGTTCAACTACATGGTTGCAGCAAAGTTGGGTATCTTGGAGCAGCGGAGAAAGCAAGAAGAGATGTTGCATAAGGAAGAGATTCGCTTACTGAGAAAGGAGATGATTCCAAGGGCTCAACTCATGCCGTTTTTCGATAGACCTTTTATTCCTCAAAGATCAAGCAGGCCTTTGACAATTCCTAAAGAACCAAGCTTCCGCATGATGAGCAGCAAGAGCTTCACTGCTTGTAGATCTGGTACCCCAATCTACAGTTTCCAACACACTACTCAAGCCCCCATCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

127

Amino Acids

14.98

Weight (kDa)

10.53

Isoelectric Point (pI)

79.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 21 - 91 1.5e-18 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 335
AccB1I GGYRCC 1 cut(s) 335
AciI CCGC 2 cut(s) 142, 295
AfaI GTAC 3 cut(s) 5, 43, 337
AgsI TTSAA 1 cut(s) 103
AloI GAACNNNNNNTCC 2 cut(s) 276, 308
AluBI AGCT 2 cut(s) 291, 315
AluI AGCT 2 cut(s) 291, 315
Alw26I GTCTC 1 cut(s) 30
AoxI GGCC 1 cut(s) 263
ApeKI GCWGC 3 cut(s) 116, 139, 306
Asp700I GAANNNNTTC 1 cut(s) 177
Asp718I GGTACC 1 cut(s) 335
BanI GGYRCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 214
BbvI GCAGC 3 cut(s) 128, 151, 318
BccI CCATC 1 cut(s) 383
BceAI ACGGC 1 cut(s) 208
BcoDI GTCTC 1 cut(s) 30
BfmI CTRYAG 1 cut(s) 346
BglII AGATCT 1 cut(s) 329
BisI GCNGC 3 cut(s) 117, 140, 307
BlsI GCNGC 3 cut(s) 118, 141, 308
BmiI GGNNCC 1 cut(s) 337
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpuEI CTTGAG 2 cut(s) 36, 351
BsaJI CCNNGG 1 cut(s) 206
Bse3DI GCAATG 1 cut(s) 102
BseDI CCNNGG 1 cut(s) 206
BseMI GCAATG 1 cut(s) 102
BseMII CTCAG 1 cut(s) 179
BseXI GCAGC 3 cut(s) 128, 151, 318
BshFI GGCC 1 cut(s) 265
BshNI GGYRCC 1 cut(s) 335
BsmAI GTCTC 1 cut(s) 30
BsnI GGCC 1 cut(s) 265
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 2 cut(s) 254, 329
BspACI CCGC 2 cut(s) 142, 295
BspANI GGCC 1 cut(s) 265
BspCNI CTCAG 1 cut(s) 180
BspLI GGNNCC 1 cut(s) 337
BspT107I GGYRCC 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 102
BssECI CCNNGG 1 cut(s) 206
BssMI GATC 2 cut(s) 254, 329
BssT1I CCWWGG 1 cut(s) 206
Bst4CI ACNGT 1 cut(s) 350
Bst6I CTCTTC 2 cut(s) 150, 168
BstC8I GCNNGC 1 cut(s) 263
BstDEI CTNAG 1 cut(s) 188
BstKTI GATC 2 cut(s) 257, 332
BstMAI GTCTC 1 cut(s) 30
BstMBI GATC 2 cut(s) 254, 329
BstMWI GCNNNNNNNGC 3 cut(s) 148, 303, 312
BstSFI CTRYAG 1 cut(s) 346
BstV1I GCAGC 3 cut(s) 128, 151, 318
BstX2I RGATCY 1 cut(s) 329
BstYI RGATCY 1 cut(s) 329
BsuRI GGCC 1 cut(s) 265
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 1 cut(s) 318
Cac8I GCNNGC 1 cut(s) 263
Csp6I GTAC 3 cut(s) 4, 42, 336
CviAII CATG 3 cut(s) 109, 220, 298
CviJI RGCY 6 cut(s) 49, 212, 265, 291, 315, 371
CviKI_1 RGCY 6 cut(s) 49, 212, 265, 291, 315, 371
CviQI GTAC 3 cut(s) 4, 42, 336
DdeI CTNAG 1 cut(s) 188
DpnI GATC 2 cut(s) 256, 331
DpnII GATC 2 cut(s) 254, 329
Eam1104I CTCTTC 2 cut(s) 150, 168
EarI CTCTTC 2 cut(s) 150, 168
Eco130I CCWWGG 1 cut(s) 206
Eco147I AGGCCT 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 206
EcoT38I GRGCYC 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 206
FaeI CATG 3 cut(s) 112, 223, 301
FaiI YATR 4 cut(s) 110, 168, 221, 299
FalI AAGNNNNNCTT 4 cut(s) 48, 80, 250, 282
FatI CATG 3 cut(s) 108, 219, 297
Fnu4HI GCNGC 3 cut(s) 117, 140, 307
FriOI GRGCYC 1 cut(s) 214
Fsp4HI GCNGC 3 cut(s) 117, 140, 307
GluI GCNGC 3 cut(s) 117, 140, 307
HaeIII GGCC 1 cut(s) 265
Hin1II CATG 3 cut(s) 112, 223, 301
HindIII AAGCTT 1 cut(s) 289
HinfI GANTC 2 cut(s) 178, 202
Hpy188I TCNGA 1 cut(s) 18
Hpy188III TCNNGA 1 cut(s) 53
HpyCH4III ACNGT 1 cut(s) 350
HpyCH4V TGCA 2 cut(s) 116, 166
HpyF10VI GCNNNNNNNGC 3 cut(s) 148, 303, 312
HpyF3I CTNAG 1 cut(s) 188
Hsp92II CATG 3 cut(s) 112, 223, 301
KpnI GGTACC 1 cut(s) 339
Kzo9I GATC 2 cut(s) 254, 329
LmnI GCTCC 1 cut(s) 136
LpnPI CCDG 2 cut(s) 247, 318
Lsp1109I GCAGC 3 cut(s) 128, 151, 318
MalI GATC 2 cut(s) 256, 331
MboI GATC 2 cut(s) 254, 329
MboII GAAGA 2 cut(s) 167, 185
MflI RGATCY 1 cut(s) 329
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 1 cut(s) 273
MmeI TCCRAC 1 cut(s) 379
MnlI CCTC 2 cut(s) 60, 258
MroXI GAANNNNTTC 1 cut(s) 177
MseI TTAA 1 cut(s) 60
MspA1I CMGCKG 1 cut(s) 142
MwoI GCNNNNNNNGC 3 cut(s) 148, 303, 312
NdeII GATC 2 cut(s) 254, 329
NlaIII CATG 3 cut(s) 112, 223, 301
NlaIV GGNNCC 1 cut(s) 337
PceI AGGCCT 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 177
PfeI GAWTC 2 cut(s) 178, 202
PkrI GCNGC 3 cut(s) 118, 141, 308
PspN4I GGNNCC 1 cut(s) 337
PsuI RGATCY 1 cut(s) 329
RsaI GTAC 3 cut(s) 5, 43, 337
RsaNI GTAC 3 cut(s) 4, 42, 336
SaqAI TTAA 1 cut(s) 60
SatI GCNGC 3 cut(s) 117, 140, 307
Sau3AI GATC 2 cut(s) 254, 329
SduI GDGCHC 1 cut(s) 214
SetI ASST 3 cut(s) 241, 293, 317
SfcI CTRYAG 1 cut(s) 346
SmlI CTYRAG 2 cut(s) 51, 366
SmoI CTYRAG 2 cut(s) 51, 366
Sse9I AATT 1 cut(s) 273
SseBI AGGCCT 1 cut(s) 265
SsiI CCGC 2 cut(s) 142, 295
StuI AGGCCT 1 cut(s) 265
StyI CCWWGG 1 cut(s) 206
TaaI ACNGT 1 cut(s) 350
TaqI TCGA 1 cut(s) 231
TasI AATT 1 cut(s) 273
TfiI GAWTC 2 cut(s) 178, 202
Tru1I TTAA 1 cut(s) 60
Tru9I TTAA 1 cut(s) 60
TscAI CASTG 1 cut(s) 325
TseI GCWGC 3 cut(s) 116, 139, 306
TspRI CASTG 1 cut(s) 325
XmnI GAANNNNTTC 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.