Rh3CG325700

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0005732
Physical Location & Seq
Reverse (-)
35473347 .. 35475952
2606 bp
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UTR
Exon/CDS
Intron
Rh3CG325700.1

Sequence Viewer

Length: 408 bp
ATGTTGAAGTCTGTGAAGAGTACTAGTCCTTCACACTCTGCTGTGAGTTCATGGAGCTCAAATTCTGGAGGAATGCCAAGAGATGAACTGAAAGAACGGGGATATGACAAAGACTACTACTATAAGGTTGATAGTCGTGTATATGCTTCTTATCAGAGCAAGTCTCCTACAAGAGAAAGTACCAAGCCTCAAGAAGTTAAACTTCACACTCAACAAAGGGCAACCAAAAGGGCAATGTTCAACTACATGGTTGCAGCAAAGTTGGGTATCTTGGAGCAGCGGAGAAAGCAAGAAGAGATGTTGCATAAGATGATAGAAGAGGAAGAGGTTCGCTTACTGAGAAAGGAGATGATTCCAAGGGCTCAACTCATGCCGTTTTTCGATAGACCTTTTATTCCTCAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

135

Amino Acids

15.95

Weight (kDa)

9.82

Isoelectric Point (pI)

74.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 67 - 135 4.1e-19 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 280
AcsI RAATTY 1 cut(s) 61
AfaI GTAC 2 cut(s) 22, 181
AgsI TTSAA 2 cut(s) 7, 241
AhlI ACTAGT 1 cut(s) 23
AluBI AGCT 1 cut(s) 57
AluI AGCT 1 cut(s) 57
Alw21I GWGCWC 1 cut(s) 59
Alw26I GTCTC 1 cut(s) 168
ApeKI GCWGC 2 cut(s) 254, 277
ApoI RAATTY 1 cut(s) 61
Asp700I GAANNNNTTC 1 cut(s) 327
BanII GRGCYC 2 cut(s) 59, 364
BarI GAAGNNNNNNTAC 2 cut(s) 13, 45
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 2 cut(s) 266, 289
BceAI ACGGC 1 cut(s) 358
BcoDI GTCTC 1 cut(s) 168
BcuI ACTAGT 1 cut(s) 23
BfaI CTAG 1 cut(s) 24
BisI GCNGC 2 cut(s) 255, 278
BlsI GCNGC 2 cut(s) 256, 279
BmcAI AGTACT 1 cut(s) 22
BplI GAGNNNNNCTC 2 cut(s) 148, 180
BpmI CTGGAG 1 cut(s) 87
BpuEI CTTGAG 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 356
Bse3DI GCAATG 1 cut(s) 240
BseDI CCNNGG 1 cut(s) 356
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 1 cut(s) 329
BseXI GCAGC 2 cut(s) 266, 289
BsiHKAI GWGCWC 1 cut(s) 59
BsmAI GTCTC 1 cut(s) 168
BsmI GAATGC 1 cut(s) 78
Bsp1286I GDGCHC 2 cut(s) 59, 364
BspACI CCGC 1 cut(s) 280
BspCNI CTCAG 1 cut(s) 330
BsrDI GCAATG 1 cut(s) 240
BssECI CCNNGG 1 cut(s) 356
BssT1I CCWWGG 1 cut(s) 356
Bst6I CTCTTC 4 cut(s) 11, 288, 312, 318
BstDEI CTNAG 1 cut(s) 338
BstMAI GTCTC 1 cut(s) 168
BstMWI GCNNNNNNNGC 1 cut(s) 286
BstV1I GCAGC 2 cut(s) 266, 289
Csp6I GTAC 2 cut(s) 21, 180
CviAII CATG 3 cut(s) 51, 247, 370
CviJI RGCY 3 cut(s) 57, 187, 362
CviKI_1 RGCY 3 cut(s) 57, 187, 362
CviQI GTAC 2 cut(s) 21, 180
DdeI CTNAG 1 cut(s) 338
Eam1104I CTCTTC 4 cut(s) 11, 288, 312, 318
EarI CTCTTC 4 cut(s) 11, 288, 312, 318
Ecl136II GAGCTC 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 356
Eco24I GRGCYC 2 cut(s) 59, 364
Eco53kI GAGCTC 1 cut(s) 57
EcoICRI GAGCTC 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 356
EcoT38I GRGCYC 2 cut(s) 59, 364
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 3 cut(s) 54, 250, 373
FaiI YATR 8 cut(s) 52, 105, 123, 142, 144, 248, 306, 371
FalI AAGNNNNNCTT 2 cut(s) 186, 218
FatI CATG 3 cut(s) 50, 246, 369
Fnu4HI GCNGC 2 cut(s) 255, 278
FriOI GRGCYC 2 cut(s) 59, 364
Fsp4HI GCNGC 2 cut(s) 255, 278
FspBI CTAG 1 cut(s) 24
GluI GCNGC 2 cut(s) 255, 278
GsuI CTGGAG 1 cut(s) 87
Hin1II CATG 3 cut(s) 54, 250, 373
HinfI GANTC 1 cut(s) 352
Hpy188I TCNGA 1 cut(s) 156
Hpy188III TCNNGA 2 cut(s) 66, 191
HpyAV CCTTC 1 cut(s) 39
HpyCH4V TGCA 2 cut(s) 254, 304
HpyF10VI GCNNNNNNNGC 1 cut(s) 286
HpyF3I CTNAG 1 cut(s) 338
Hsp92II CATG 3 cut(s) 54, 250, 373
LmnI GCTCC 2 cut(s) 54, 274
LpnPI CCDG 1 cut(s) 51
Lsp1109I GCAGC 2 cut(s) 266, 289
MaeI CTAG 1 cut(s) 24
MboII GAAGA 4 cut(s) 28, 305, 329, 335
MhlI GDGCHC 2 cut(s) 59, 364
MluCI AATT 1 cut(s) 61
MnlI CCTC 5 cut(s) 62, 198, 313, 319, 408
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 1 cut(s) 198
MspA1I CMGCKG 1 cut(s) 280
Mva1269I GAATGC 1 cut(s) 78
MwoI GCNNNNNNNGC 1 cut(s) 286
NlaIII CATG 3 cut(s) 54, 250, 373
PctI GAATGC 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 1 cut(s) 352
PkrI GCNGC 2 cut(s) 256, 279
Psp124BI GAGCTC 1 cut(s) 59
RsaI GTAC 2 cut(s) 22, 181
RsaNI GTAC 2 cut(s) 21, 180
SacI GAGCTC 1 cut(s) 59
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 2 cut(s) 255, 278
ScaI AGTACT 1 cut(s) 22
SduI GDGCHC 2 cut(s) 59, 364
SetI ASST 5 cut(s) 59, 129, 330, 391, 407
SmlI CTYRAG 1 cut(s) 189
SmoI CTYRAG 1 cut(s) 189
SpeI ACTAGT 1 cut(s) 23
Sse9I AATT 1 cut(s) 61
SsiI CCGC 1 cut(s) 280
SspMI CTAG 1 cut(s) 24
SstI GAGCTC 1 cut(s) 59
StyI CCWWGG 1 cut(s) 356
TaqI TCGA 1 cut(s) 381
TasI AATT 1 cut(s) 61
TatI WGTACW 1 cut(s) 20
TfiI GAWTC 1 cut(s) 352
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TseI GCWGC 2 cut(s) 254, 277
TspDTI ATGAA 2 cut(s) 39, 99
XapI RAATTY 1 cut(s) 61
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 1 cut(s) 24
ZrmI AGTACT 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.