Rh3BG329000

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0005732
Physical Location & Seq
Reverse (-)
34372022 .. 34374974
2953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG329000.1

Sequence Viewer

Length: 519 bp
ATGTTGAAGTCTGTGAAGAGTACTAGTCCTTCACACTCTGCTGTGAGTTCATGGAGCTCAAATTCTGGAGGAATGCCAAGAGATGAACTCAAAGAACGGGGATATGACAAAGACTACTACTATAAGGCTTCTTATCAGAGCAAGTCTCCTACAAGAGAAAGTACCAAGCCTCAAGAAATTAAACTTCACACTCAACAAAGGGCAACCAAACGGGCAATGTTCAACTACATGGTTGCAGCAAAGCTGAGTATCTTGGAGCAGCGGAGAAAGCAAGAAGAGATGTTGCATAAGATGATAGAAGAGGAAGAGATTCGCTTACTGAGAAAGGAGATGATTCCAAGGGCTCAACTCATGCCGTTTTTCGATAGACCTTTTATTCCTCAAAGATCAAGCAGGCCTTTGACAATTCCTAAAGAACCAAGCTGCTTCCGCATGATGAGCAGCAAGGGCTTCACTGCTTGTAGATCTGGTACCCCAATCTACAGTTTCCAACACACTACTCAAGCCCCCATCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

172

Amino Acids

20.05

Weight (kDa)

9.97

Isoelectric Point (pI)

78.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 60 - 135 1.8e-22 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 470
AccB1I GGYRCC 1 cut(s) 470
AciI CCGC 2 cut(s) 262, 430
AcsI RAATTY 1 cut(s) 61
AfaI GTAC 3 cut(s) 22, 163, 472
AgsI TTSAA 2 cut(s) 7, 223
AhlI ACTAGT 1 cut(s) 23
AluBI AGCT 3 cut(s) 57, 244, 423
AluI AGCT 3 cut(s) 57, 244, 423
Alw21I GWGCWC 1 cut(s) 59
Alw26I GTCTC 1 cut(s) 150
AoxI GGCC 1 cut(s) 395
ApeKI GCWGC 4 cut(s) 236, 259, 423, 441
ApoI RAATTY 1 cut(s) 61
Asp700I GAANNNNTTC 1 cut(s) 309
Asp718I GGTACC 1 cut(s) 470
BanI GGYRCC 1 cut(s) 470
BanII GRGCYC 2 cut(s) 59, 346
BarI GAAGNNNNNNTAC 2 cut(s) 13, 45
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 4 cut(s) 248, 271, 410, 453
BccI CCATC 1 cut(s) 518
BceAI ACGGC 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 150
BcuI ACTAGT 1 cut(s) 23
BfaI CTAG 1 cut(s) 24
BfmI CTRYAG 1 cut(s) 481
BglII AGATCT 1 cut(s) 464
BisI GCNGC 4 cut(s) 237, 260, 424, 442
BlsI GCNGC 4 cut(s) 238, 261, 425, 443
BmcAI AGTACT 1 cut(s) 22
BmiI GGNNCC 1 cut(s) 472
BplI GAGNNNNNCTC 4 cut(s) 72, 104, 130, 162
BpmI CTGGAG 1 cut(s) 87
BpuEI CTTGAG 2 cut(s) 156, 486
BsaJI CCNNGG 1 cut(s) 338
Bse3DI GCAATG 1 cut(s) 222
BseDI CCNNGG 1 cut(s) 338
BseMI GCAATG 1 cut(s) 222
BseMII CTCAG 2 cut(s) 236, 311
BseXI GCAGC 4 cut(s) 248, 271, 410, 453
BshFI GGCC 1 cut(s) 397
BshNI GGYRCC 1 cut(s) 470
BsiHKAI GWGCWC 1 cut(s) 59
BsmAI GTCTC 1 cut(s) 150
BsmI GAATGC 1 cut(s) 78
BsnI GGCC 1 cut(s) 397
Bsp1286I GDGCHC 2 cut(s) 59, 346
Bsp143I GATC 2 cut(s) 386, 464
BspACI CCGC 2 cut(s) 262, 430
BspANI GGCC 1 cut(s) 397
BspCNI CTCAG 2 cut(s) 237, 312
BspLI GGNNCC 1 cut(s) 472
BspT107I GGYRCC 1 cut(s) 470
BsrDI GCAATG 1 cut(s) 222
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 2 cut(s) 386, 464
BssT1I CCWWGG 1 cut(s) 338
Bst4CI ACNGT 1 cut(s) 485
Bst6I CTCTTC 4 cut(s) 11, 270, 294, 300
BstC8I GCNNGC 1 cut(s) 395
BstDEI CTNAG 2 cut(s) 245, 320
BstKTI GATC 2 cut(s) 389, 467
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 2 cut(s) 386, 464
BstMWI GCNNNNNNNGC 4 cut(s) 268, 429, 438, 447
BstSFI CTRYAG 1 cut(s) 481
BstV1I GCAGC 4 cut(s) 248, 271, 410, 453
BstX2I RGATCY 1 cut(s) 464
BstYI RGATCY 1 cut(s) 464
BsuRI GGCC 1 cut(s) 397
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 1 cut(s) 453
Cac8I GCNNGC 1 cut(s) 395
Csp6I GTAC 3 cut(s) 21, 162, 471
CviAII CATG 4 cut(s) 51, 229, 352, 433
CviJI RGCY 9 cut(s) 57, 128, 169, 244, 344, 397, 423, 450, 506
CviKI_1 RGCY 9 cut(s) 57, 128, 169, 244, 344, 397, 423, 450, 506
CviQI GTAC 3 cut(s) 21, 162, 471
DdeI CTNAG 2 cut(s) 245, 320
DpnI GATC 2 cut(s) 388, 466
DpnII GATC 2 cut(s) 386, 464
Eam1104I CTCTTC 4 cut(s) 11, 270, 294, 300
EarI CTCTTC 4 cut(s) 11, 270, 294, 300
Ecl136II GAGCTC 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 338
Eco147I AGGCCT 1 cut(s) 397
Eco24I GRGCYC 2 cut(s) 59, 346
Eco53kI GAGCTC 1 cut(s) 57
EcoICRI GAGCTC 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 338
EcoT38I GRGCYC 2 cut(s) 59, 346
ErhI CCWWGG 1 cut(s) 338
FaeI CATG 4 cut(s) 54, 232, 355, 436
FaiI YATR 7 cut(s) 52, 105, 123, 230, 288, 353, 434
FalI AAGNNNNNCTT 2 cut(s) 382, 414
FatI CATG 4 cut(s) 50, 228, 351, 432
Fnu4HI GCNGC 4 cut(s) 237, 260, 424, 442
FriOI GRGCYC 2 cut(s) 59, 346
Fsp4HI GCNGC 4 cut(s) 237, 260, 424, 442
FspBI CTAG 1 cut(s) 24
GluI GCNGC 4 cut(s) 237, 260, 424, 442
GsuI CTGGAG 1 cut(s) 87
HaeIII GGCC 1 cut(s) 397
Hin1II CATG 4 cut(s) 54, 232, 355, 436
HinfI GANTC 2 cut(s) 310, 334
Hpy188I TCNGA 1 cut(s) 138
Hpy188III TCNNGA 2 cut(s) 66, 173
HpyAV CCTTC 1 cut(s) 39
HpyCH4III ACNGT 1 cut(s) 485
HpyCH4V TGCA 2 cut(s) 236, 286
HpyF10VI GCNNNNNNNGC 4 cut(s) 268, 429, 438, 447
HpyF3I CTNAG 2 cut(s) 245, 320
Hsp92II CATG 4 cut(s) 54, 232, 355, 436
KpnI GGTACC 1 cut(s) 474
Kzo9I GATC 2 cut(s) 386, 464
LmnI GCTCC 2 cut(s) 54, 256
LpnPI CCDG 3 cut(s) 51, 379, 453
Lsp1109I GCAGC 4 cut(s) 248, 271, 410, 453
MaeI CTAG 1 cut(s) 24
MalI GATC 2 cut(s) 388, 466
MboI GATC 2 cut(s) 386, 464
MboII GAAGA 4 cut(s) 28, 287, 311, 317
MflI RGATCY 1 cut(s) 464
MhlI GDGCHC 2 cut(s) 59, 346
MluCI AATT 3 cut(s) 61, 177, 405
MmeI TCCRAC 1 cut(s) 514
MnlI CCTC 4 cut(s) 62, 180, 295, 390
MroXI GAANNNNTTC 1 cut(s) 309
MseI TTAA 1 cut(s) 180
MspA1I CMGCKG 1 cut(s) 262
Mva1269I GAATGC 1 cut(s) 78
MwoI GCNNNNNNNGC 4 cut(s) 268, 429, 438, 447
NdeII GATC 2 cut(s) 386, 464
NlaIII CATG 4 cut(s) 54, 232, 355, 436
NlaIV GGNNCC 1 cut(s) 472
PceI AGGCCT 1 cut(s) 397
PctI GAATGC 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 309
PfeI GAWTC 2 cut(s) 310, 334
PkrI GCNGC 4 cut(s) 238, 261, 425, 443
Psp124BI GAGCTC 1 cut(s) 59
PspN4I GGNNCC 1 cut(s) 472
PsuI RGATCY 1 cut(s) 464
RsaI GTAC 3 cut(s) 22, 163, 472
RsaNI GTAC 3 cut(s) 21, 162, 471
SacI GAGCTC 1 cut(s) 59
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 4 cut(s) 237, 260, 424, 442
Sau3AI GATC 2 cut(s) 386, 464
ScaI AGTACT 1 cut(s) 22
SduI GDGCHC 2 cut(s) 59, 346
SetI ASST 4 cut(s) 59, 246, 373, 425
SfcI CTRYAG 1 cut(s) 481
SmlI CTYRAG 2 cut(s) 171, 501
SmoI CTYRAG 2 cut(s) 171, 501
SpeI ACTAGT 1 cut(s) 23
Sse9I AATT 3 cut(s) 61, 177, 405
SseBI AGGCCT 1 cut(s) 397
SsiI CCGC 2 cut(s) 262, 430
SspMI CTAG 1 cut(s) 24
SstI GAGCTC 1 cut(s) 59
StuI AGGCCT 1 cut(s) 397
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 1 cut(s) 485
TaqI TCGA 1 cut(s) 363
TasI AATT 3 cut(s) 61, 177, 405
TatI WGTACW 1 cut(s) 20
TfiI GAWTC 2 cut(s) 310, 334
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TscAI CASTG 1 cut(s) 460
TseI GCWGC 4 cut(s) 236, 259, 423, 441
TspDTI ATGAA 2 cut(s) 39, 99
TspRI CASTG 1 cut(s) 460
XapI RAATTY 1 cut(s) 61
XmnI GAANNNNTTC 1 cut(s) 309
XspI CTAG 1 cut(s) 24
ZrmI AGTACT 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.