RLG00000022892

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005732
Physical Location & Seq
Forward (+)
14225077 .. 14227401
2325 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022892

Sequence Viewer

Length: 540 bp
ATGGAGAAGGCACTCACCAAGTCTAGTCTTAAGTCTGTGAAGAGTACTAGTCCTTTACACTCTGCTGTGAGTTCATGGAGCTCAAATTCTGGAGGAATGCCAAGAGATGAACTCAAAGAGCAGGGATATGACAAAGACTACTACTATAAGGCTTCTTATCAGAGCAAGTCTCCTACAAGAGAAAGTACCAAGCCTCAAGAAGTTAAACTTCACACTCAACAAAGGGCAACCAAACGGGCAATGTTCAACTACACGGTTGCGGCAAAGCTGAGTATCTTGGAGCAGCGGAGAAAGCAAGAAGAGATGTTGCATAAGATGATAGAAGAGGAAGAGATTCGCTTACTGAGAAAGGAGATGATTCCAAGGGCTCAACTCATGCCGTTTTTCGATAGACCTTTTATTCCTCAAAGATCAAGCAGGCCTTTGACAATTCCTAAAGAACCAAGCTTCCGCATGATAAGCAGCAAGAGCTTCACTGCTTGTAGATCTGGTACCCCAATCTACAGTTTCCAACACACTACTCAAGCCCCCATCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

180

Amino Acids

20.75

Weight (kDa)

9.96

Isoelectric Point (pI)

70.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 69 - 143 3e-22 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 491
AccB1I GGYRCC 1 cut(s) 491
AciI CCGC 3 cut(s) 260, 286, 451
AcsI RAATTY 1 cut(s) 85
AfaI GTAC 3 cut(s) 46, 187, 493
AflII CTTAAG 1 cut(s) 29
AgsI TTSAA 1 cut(s) 247
AhlI ACTAGT 1 cut(s) 47
AloI GAACNNNNNNTCC 2 cut(s) 432, 464
AluBI AGCT 4 cut(s) 81, 268, 447, 471
AluI AGCT 4 cut(s) 81, 268, 447, 471
Alw21I GWGCWC 1 cut(s) 83
Alw26I GTCTC 1 cut(s) 174
AoxI GGCC 1 cut(s) 419
ApeKI GCWGC 2 cut(s) 283, 462
ApoI RAATTY 1 cut(s) 85
Asp700I GAANNNNTTC 1 cut(s) 333
Asp718I GGTACC 1 cut(s) 491
AsuHPI GGTGA 1 cut(s) 7
BanI GGYRCC 1 cut(s) 491
BanII GRGCYC 2 cut(s) 83, 370
Bbv12I GWGCWC 1 cut(s) 83
BbvI GCAGC 2 cut(s) 295, 474
BccI CCATC 1 cut(s) 539
BceAI ACGGC 1 cut(s) 364
BcoDI GTCTC 1 cut(s) 174
BcuI ACTAGT 1 cut(s) 47
BfaI CTAG 2 cut(s) 24, 48
BfmI CTRYAG 1 cut(s) 502
BfrI CTTAAG 1 cut(s) 29
BglII AGATCT 1 cut(s) 485
BisI GCNGC 3 cut(s) 261, 284, 463
BlsI GCNGC 3 cut(s) 262, 285, 464
BmcAI AGTACT 1 cut(s) 46
BmiI GGNNCC 1 cut(s) 493
BplI GAGNNNNNCTC 4 cut(s) 96, 128, 154, 186
BpmI CTGGAG 1 cut(s) 111
BpuEI CTTGAG 2 cut(s) 180, 507
BsaJI CCNNGG 1 cut(s) 362
Bse3DI GCAATG 1 cut(s) 246
BseDI CCNNGG 1 cut(s) 362
BseMI GCAATG 1 cut(s) 246
BseMII CTCAG 2 cut(s) 260, 335
BseXI GCAGC 2 cut(s) 295, 474
BshFI GGCC 1 cut(s) 421
BshNI GGYRCC 1 cut(s) 491
BsiHKAI GWGCWC 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 174
BsmI GAATGC 1 cut(s) 102
BsnI GGCC 1 cut(s) 421
Bsp1286I GDGCHC 2 cut(s) 83, 370
Bsp143I GATC 2 cut(s) 410, 485
BspACI CCGC 3 cut(s) 260, 286, 451
BspANI GGCC 1 cut(s) 421
BspCNI CTCAG 2 cut(s) 261, 336
BspLI GGNNCC 1 cut(s) 493
BspT107I GGYRCC 1 cut(s) 491
BspTI CTTAAG 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 246
BssECI CCNNGG 1 cut(s) 362
BssMI GATC 2 cut(s) 410, 485
BssT1I CCWWGG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 256, 506
Bst6I CTCTTC 4 cut(s) 35, 294, 318, 324
BstAFI CTTAAG 1 cut(s) 29
BstC8I GCNNGC 1 cut(s) 419
BstDEI CTNAG 2 cut(s) 269, 344
BstKTI GATC 2 cut(s) 413, 488
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 2 cut(s) 410, 485
BstMWI GCNNNNNNNGC 3 cut(s) 292, 459, 468
BstSFI CTRYAG 1 cut(s) 502
BstV1I GCAGC 2 cut(s) 295, 474
BstX2I RGATCY 1 cut(s) 485
BstYI RGATCY 1 cut(s) 485
BsuRI GGCC 1 cut(s) 421
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 1 cut(s) 474
Cac8I GCNNGC 1 cut(s) 419
Csp6I GTAC 3 cut(s) 45, 186, 492
CviAII CATG 3 cut(s) 75, 376, 454
CviJI RGCY 9 cut(s) 81, 152, 193, 268, 368, 421, 447, 471, 527
CviKI_1 RGCY 9 cut(s) 81, 152, 193, 268, 368, 421, 447, 471, 527
CviQI GTAC 3 cut(s) 45, 186, 492
DdeI CTNAG 2 cut(s) 269, 344
DpnI GATC 2 cut(s) 412, 487
DpnII GATC 2 cut(s) 410, 485
Eam1104I CTCTTC 4 cut(s) 35, 294, 318, 324
EarI CTCTTC 4 cut(s) 35, 294, 318, 324
Ecl136II GAGCTC 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 362
Eco147I AGGCCT 1 cut(s) 421
Eco24I GRGCYC 2 cut(s) 83, 370
Eco53kI GAGCTC 1 cut(s) 81
EcoICRI GAGCTC 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 362
EcoT38I GRGCYC 2 cut(s) 83, 370
ErhI CCWWGG 1 cut(s) 362
FaeI CATG 3 cut(s) 78, 379, 457
FaiI YATR 6 cut(s) 76, 129, 147, 312, 377, 455
FalI AAGNNNNNCTT 4 cut(s) 192, 224, 406, 438
FatI CATG 3 cut(s) 74, 375, 453
Fnu4HI GCNGC 3 cut(s) 261, 284, 463
FriOI GRGCYC 2 cut(s) 83, 370
Fsp4HI GCNGC 3 cut(s) 261, 284, 463
FspBI CTAG 2 cut(s) 24, 48
GluI GCNGC 3 cut(s) 261, 284, 463
GsuI CTGGAG 1 cut(s) 111
HaeIII GGCC 1 cut(s) 421
Hin1II CATG 3 cut(s) 78, 379, 457
HindIII AAGCTT 1 cut(s) 445
HinfI GANTC 2 cut(s) 334, 358
HphI GGTGA 1 cut(s) 7
Hpy188I TCNGA 1 cut(s) 162
Hpy188III TCNNGA 2 cut(s) 90, 197
HpyCH4III ACNGT 2 cut(s) 256, 506
HpyCH4V TGCA 1 cut(s) 310
HpyF10VI GCNNNNNNNGC 3 cut(s) 292, 459, 468
HpyF3I CTNAG 2 cut(s) 269, 344
Hsp92II CATG 3 cut(s) 78, 379, 457
KpnI GGTACC 1 cut(s) 495
Kzo9I GATC 2 cut(s) 410, 485
LmnI GCTCC 2 cut(s) 78, 280
LpnPI CCDG 4 cut(s) 75, 107, 403, 474
Lsp1109I GCAGC 2 cut(s) 295, 474
MaeI CTAG 2 cut(s) 24, 48
MalI GATC 2 cut(s) 412, 487
MboI GATC 2 cut(s) 410, 485
MboII GAAGA 4 cut(s) 52, 311, 335, 341
MflI RGATCY 1 cut(s) 485
MhlI GDGCHC 2 cut(s) 83, 370
MluCI AATT 2 cut(s) 85, 429
MmeI TCCRAC 1 cut(s) 535
MnlI CCTC 4 cut(s) 86, 204, 319, 414
MroXI GAANNNNTTC 1 cut(s) 333
MseI TTAA 2 cut(s) 30, 204
MspA1I CMGCKG 1 cut(s) 286
MspCI CTTAAG 1 cut(s) 29
Mva1269I GAATGC 1 cut(s) 102
MwoI GCNNNNNNNGC 3 cut(s) 292, 459, 468
NdeII GATC 2 cut(s) 410, 485
NlaIII CATG 3 cut(s) 78, 379, 457
NlaIV GGNNCC 1 cut(s) 493
PceI AGGCCT 1 cut(s) 421
PctI GAATGC 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 333
PfeI GAWTC 2 cut(s) 334, 358
PkrI GCNGC 3 cut(s) 262, 285, 464
Psp124BI GAGCTC 1 cut(s) 83
PspN4I GGNNCC 1 cut(s) 493
PsuI RGATCY 1 cut(s) 485
RsaI GTAC 3 cut(s) 46, 187, 493
RsaNI GTAC 3 cut(s) 45, 186, 492
SacI GAGCTC 1 cut(s) 83
SaqAI TTAA 2 cut(s) 30, 204
SatI GCNGC 3 cut(s) 261, 284, 463
Sau3AI GATC 2 cut(s) 410, 485
ScaI AGTACT 1 cut(s) 46
SduI GDGCHC 2 cut(s) 83, 370
SetI ASST 5 cut(s) 83, 270, 397, 449, 473
SfcI CTRYAG 1 cut(s) 502
SmlI CTYRAG 3 cut(s) 29, 195, 522
SmoI CTYRAG 3 cut(s) 29, 195, 522
SpeI ACTAGT 1 cut(s) 47
Sse9I AATT 2 cut(s) 85, 429
SseBI AGGCCT 1 cut(s) 421
SsiI CCGC 3 cut(s) 260, 286, 451
SspMI CTAG 2 cut(s) 24, 48
SstI GAGCTC 1 cut(s) 83
StuI AGGCCT 1 cut(s) 421
StyI CCWWGG 1 cut(s) 362
TaaI ACNGT 2 cut(s) 256, 506
TaqI TCGA 1 cut(s) 387
TasI AATT 2 cut(s) 85, 429
TatI WGTACW 1 cut(s) 44
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 2 cut(s) 334, 358
Tru1I TTAA 2 cut(s) 30, 204
Tru9I TTAA 2 cut(s) 30, 204
TscAI CASTG 1 cut(s) 481
TseI GCWGC 2 cut(s) 283, 462
TspDTI ATGAA 2 cut(s) 63, 123
TspRI CASTG 1 cut(s) 481
Vha464I CTTAAG 1 cut(s) 29
XapI RAATTY 1 cut(s) 85
XmnI GAANNNNTTC 1 cut(s) 333
XspI CTAG 2 cut(s) 24, 48
ZrmI AGTACT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.