Rw3G025830

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005732
Physical Location & Seq
Reverse (-)
34864877 .. 34867569
2693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025830.1

Sequence Viewer

Length: 558 bp
ATGGAGAAGGCACTCACCAAGTCTAGTCTTAAGTCTGTGAAGAGTACTAGTCCTTCACACTCTGCTGTGAGTTCATGGAGCTCAAATTCTGGAGGAATGCCAAGAGATGAACTGAAAGAACGGGGATATGACAAAGACTACTACTATAAGGTTGATAGTCGTGTATATGCTTCTTATCAGAGCAAGTCTCCTACAAGAGAAAGTACCAAGCCTCAAGAAGTTAAACTTCACACTCAACAAAGGGCAACCAAAAGGGCAATGTTCAACTACATGGTTGCAGCAAAGTTGGGTATCTTGGAGCAGCGGAGAAAGCAAGAAGAGATGTTGCATAAGATGATAGAAGAGGAAGAGGTTCGCTTACTGAGAAAGGAGATGATTCCAAGGGCTCAACTCATGCCGTTTTTCGATAGACCTTTTATTCCTCAAAGATCAAGCAGGCCTTTGACAATTCCTAAAGAACCAAGCTTCCGCATGATGAGCAGCAAGAGCTTCACTGCTTGTAGATCTGGTACCCCAATCTACAGTTTCCAACACACTACTCAAGCCCCCATCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

185

Amino Acids

21.48

Weight (kDa)

10.0

Isoelectric Point (pI)

73.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 75 - 149 1.3e-21 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 509
AccB1I GGYRCC 1 cut(s) 509
AciI CCGC 2 cut(s) 304, 469
AcsI RAATTY 1 cut(s) 85
AfaI GTAC 3 cut(s) 46, 205, 511
AflII CTTAAG 1 cut(s) 29
AgsI TTSAA 1 cut(s) 265
AhlI ACTAGT 1 cut(s) 47
AloI GAACNNNNNNTCC 2 cut(s) 450, 482
AluBI AGCT 3 cut(s) 81, 465, 489
AluI AGCT 3 cut(s) 81, 465, 489
Alw21I GWGCWC 1 cut(s) 83
Alw26I GTCTC 1 cut(s) 192
AoxI GGCC 1 cut(s) 437
ApeKI GCWGC 3 cut(s) 278, 301, 480
ApoI RAATTY 1 cut(s) 85
Asp700I GAANNNNTTC 1 cut(s) 351
Asp718I GGTACC 1 cut(s) 509
AsuHPI GGTGA 1 cut(s) 7
BanI GGYRCC 1 cut(s) 509
BanII GRGCYC 2 cut(s) 83, 388
BarI GAAGNNNNNNTAC 2 cut(s) 37, 69
Bbv12I GWGCWC 1 cut(s) 83
BbvI GCAGC 3 cut(s) 290, 313, 492
BccI CCATC 1 cut(s) 557
BceAI ACGGC 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 192
BcuI ACTAGT 1 cut(s) 47
BfaI CTAG 2 cut(s) 24, 48
BfmI CTRYAG 1 cut(s) 520
BfrI CTTAAG 1 cut(s) 29
BglII AGATCT 1 cut(s) 503
BisI GCNGC 3 cut(s) 279, 302, 481
BlsI GCNGC 3 cut(s) 280, 303, 482
BmcAI AGTACT 1 cut(s) 46
BmiI GGNNCC 1 cut(s) 511
BplI GAGNNNNNCTC 2 cut(s) 172, 204
BpmI CTGGAG 1 cut(s) 111
BpuEI CTTGAG 2 cut(s) 198, 525
BsaJI CCNNGG 1 cut(s) 380
Bse3DI GCAATG 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 380
BseMI GCAATG 1 cut(s) 264
BseMII CTCAG 1 cut(s) 353
BseXI GCAGC 3 cut(s) 290, 313, 492
BshFI GGCC 1 cut(s) 439
BshNI GGYRCC 1 cut(s) 509
BsiHKAI GWGCWC 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 192
BsmI GAATGC 1 cut(s) 102
BsnI GGCC 1 cut(s) 439
Bsp1286I GDGCHC 2 cut(s) 83, 388
Bsp143I GATC 2 cut(s) 428, 503
BspACI CCGC 2 cut(s) 304, 469
BspANI GGCC 1 cut(s) 439
BspCNI CTCAG 1 cut(s) 354
BspLI GGNNCC 1 cut(s) 511
BspT107I GGYRCC 1 cut(s) 509
BspTI CTTAAG 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 264
BssECI CCNNGG 1 cut(s) 380
BssMI GATC 2 cut(s) 428, 503
BssT1I CCWWGG 1 cut(s) 380
Bst4CI ACNGT 1 cut(s) 524
Bst6I CTCTTC 4 cut(s) 35, 312, 336, 342
BstAFI CTTAAG 1 cut(s) 29
BstC8I GCNNGC 1 cut(s) 437
BstDEI CTNAG 1 cut(s) 362
BstKTI GATC 2 cut(s) 431, 506
BstMAI GTCTC 1 cut(s) 192
BstMBI GATC 2 cut(s) 428, 503
BstMWI GCNNNNNNNGC 3 cut(s) 310, 477, 486
BstSFI CTRYAG 1 cut(s) 520
BstV1I GCAGC 3 cut(s) 290, 313, 492
BstX2I RGATCY 1 cut(s) 503
BstYI RGATCY 1 cut(s) 503
BsuRI GGCC 1 cut(s) 439
BtsI GCAGTG 1 cut(s) 492
BtsIMutI CAGTG 1 cut(s) 492
Cac8I GCNNGC 1 cut(s) 437
Csp6I GTAC 3 cut(s) 45, 204, 510
CviAII CATG 4 cut(s) 75, 271, 394, 472
CviJI RGCY 7 cut(s) 81, 211, 386, 439, 465, 489, 545
CviKI_1 RGCY 7 cut(s) 81, 211, 386, 439, 465, 489, 545
CviQI GTAC 3 cut(s) 45, 204, 510
DdeI CTNAG 1 cut(s) 362
DpnI GATC 2 cut(s) 430, 505
DpnII GATC 2 cut(s) 428, 503
Eam1104I CTCTTC 4 cut(s) 35, 312, 336, 342
EarI CTCTTC 4 cut(s) 35, 312, 336, 342
Ecl136II GAGCTC 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 380
Eco147I AGGCCT 1 cut(s) 439
Eco24I GRGCYC 2 cut(s) 83, 388
Eco53kI GAGCTC 1 cut(s) 81
EcoICRI GAGCTC 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 380
EcoT38I GRGCYC 2 cut(s) 83, 388
ErhI CCWWGG 1 cut(s) 380
FaeI CATG 4 cut(s) 78, 274, 397, 475
FaiI YATR 9 cut(s) 76, 129, 147, 166, 168, 272, 330, 395, 473
FalI AAGNNNNNCTT 4 cut(s) 210, 242, 424, 456
FatI CATG 4 cut(s) 74, 270, 393, 471
Fnu4HI GCNGC 3 cut(s) 279, 302, 481
FriOI GRGCYC 2 cut(s) 83, 388
Fsp4HI GCNGC 3 cut(s) 279, 302, 481
FspBI CTAG 2 cut(s) 24, 48
GluI GCNGC 3 cut(s) 279, 302, 481
GsuI CTGGAG 1 cut(s) 111
HaeIII GGCC 1 cut(s) 439
Hin1II CATG 4 cut(s) 78, 274, 397, 475
HindIII AAGCTT 1 cut(s) 463
HinfI GANTC 1 cut(s) 376
HphI GGTGA 1 cut(s) 7
Hpy188I TCNGA 1 cut(s) 180
Hpy188III TCNNGA 2 cut(s) 90, 215
HpyAV CCTTC 1 cut(s) 63
HpyCH4III ACNGT 1 cut(s) 524
HpyCH4V TGCA 2 cut(s) 278, 328
HpyF10VI GCNNNNNNNGC 3 cut(s) 310, 477, 486
HpyF3I CTNAG 1 cut(s) 362
Hsp92II CATG 4 cut(s) 78, 274, 397, 475
KpnI GGTACC 1 cut(s) 513
Kzo9I GATC 2 cut(s) 428, 503
LmnI GCTCC 2 cut(s) 78, 298
LpnPI CCDG 3 cut(s) 75, 421, 492
Lsp1109I GCAGC 3 cut(s) 290, 313, 492
MaeI CTAG 2 cut(s) 24, 48
MalI GATC 2 cut(s) 430, 505
MboI GATC 2 cut(s) 428, 503
MboII GAAGA 4 cut(s) 52, 329, 353, 359
MflI RGATCY 1 cut(s) 503
MhlI GDGCHC 2 cut(s) 83, 388
MluCI AATT 2 cut(s) 85, 447
MmeI TCCRAC 1 cut(s) 553
MnlI CCTC 5 cut(s) 86, 222, 337, 343, 432
MroXI GAANNNNTTC 1 cut(s) 351
MseI TTAA 2 cut(s) 30, 222
MspA1I CMGCKG 1 cut(s) 304
MspCI CTTAAG 1 cut(s) 29
Mva1269I GAATGC 1 cut(s) 102
MwoI GCNNNNNNNGC 3 cut(s) 310, 477, 486
NdeII GATC 2 cut(s) 428, 503
NlaIII CATG 4 cut(s) 78, 274, 397, 475
NlaIV GGNNCC 1 cut(s) 511
PceI AGGCCT 1 cut(s) 439
PctI GAATGC 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 351
PfeI GAWTC 1 cut(s) 376
PkrI GCNGC 3 cut(s) 280, 303, 482
Psp124BI GAGCTC 1 cut(s) 83
PspN4I GGNNCC 1 cut(s) 511
PsuI RGATCY 1 cut(s) 503
RsaI GTAC 3 cut(s) 46, 205, 511
RsaNI GTAC 3 cut(s) 45, 204, 510
SacI GAGCTC 1 cut(s) 83
SaqAI TTAA 2 cut(s) 30, 222
SatI GCNGC 3 cut(s) 279, 302, 481
Sau3AI GATC 2 cut(s) 428, 503
ScaI AGTACT 1 cut(s) 46
SduI GDGCHC 2 cut(s) 83, 388
SetI ASST 6 cut(s) 83, 153, 354, 415, 467, 491
SfcI CTRYAG 1 cut(s) 520
SmlI CTYRAG 3 cut(s) 29, 213, 540
SmoI CTYRAG 3 cut(s) 29, 213, 540
SpeI ACTAGT 1 cut(s) 47
Sse9I AATT 2 cut(s) 85, 447
SseBI AGGCCT 1 cut(s) 439
SsiI CCGC 2 cut(s) 304, 469
SspMI CTAG 2 cut(s) 24, 48
SstI GAGCTC 1 cut(s) 83
StuI AGGCCT 1 cut(s) 439
StyI CCWWGG 1 cut(s) 380
TaaI ACNGT 1 cut(s) 524
TaqI TCGA 1 cut(s) 405
TasI AATT 2 cut(s) 85, 447
TatI WGTACW 1 cut(s) 44
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 2 cut(s) 30, 222
Tru9I TTAA 2 cut(s) 30, 222
TscAI CASTG 1 cut(s) 499
TseI GCWGC 3 cut(s) 278, 301, 480
TspDTI ATGAA 2 cut(s) 63, 123
TspRI CASTG 1 cut(s) 499
Vha464I CTTAAG 1 cut(s) 29
XapI RAATTY 1 cut(s) 85
XmnI GAANNNNTTC 1 cut(s) 351
XspI CTAG 2 cut(s) 24, 48
ZrmI AGTACT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.