AT5G40270

Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002219
Physical Location & Seq
Reverse (-)
16093035 .. 16097245
4211 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G40270.1

Sequence Viewer

Length: 1422 bp
ATGGGAGCGTATTGCGACGAAAACCTAAGTAGTTTACCGGTGTTCTCCTCCGGCGCTCCGGCGAACGAGCTCCGGTTCAGCAAGCATGTCTACGATAACGTTCATGGAAACATCTATCTCGATCCTCTTTGTTTAAAATTCATTGACACTGAGCAGTTTCAGAGGCTTCGTGAACTAAAGCAACTTGGAGTGACAAACATGGTATATCCAGGAGCTGTACATTCGAGATTTGAGCACTCTCTAGGCGTGTATTGGCTTGCAGGCGAAACTGCTCAGAGGCTAAAAAATTTCCAGGGAATGGAGCTTGGTATTGACAATTATGATCTTCAGACTGTGAGACTTGCTGGACTTCTCCATGATATTGGCCATGGGCCTTTCAGTCATATGTTTGAGCGTGAATTCCTTCCAAAGGTCATAAGTGACTGTCAGTGGTCTCATGAGTTAATGTCTGTAAACATGATTGACCATATGGTTGATACACATCACATCGATATTGATGCTCAAATGCTGAAAAGAGTGAAGGACATGATACTAGCTAGTACTGAATTTTCACAGCTGAAAGGCAATGCAGAGAAGCGTTTCTTGTATGATATTGTTGCTAATGGTCGAAATGGGATTGATGTGGACAAGTTTGACTACCTTGTCCGTGATTCTCGAGCTTGTGGACTGGGATCCAATTTTCAATTCCAAAGACTGACAGAGACGATGAGAGTCCTGGACAATGAAATCTGCTTCCGCGCCAAGGAGTATCTTAGTGTCCACAAGTTATTTGCAACTCGAGCTGATCTTTATCGAACCGTCTACACGCATTCGAAAGTAAAGGCGATAGAGCTGATGATAGTAGATGCCATGGTCAAAGCCAACAATCATTTGGAAATTTCTTCTATGATTAATGACCCATCTGAATACTGGAAGTTAGACGACACAATCCTCAAAACTATCGAAATAGCTCCAGATCCAGAACTTGCAGAAGCGAAAGAGCTGATACTCCGTGTTCGCAGAAGGCAGTTGTACCAATTCTGTAACGAATATGCGGTTCCAAAGGACAAAATCGATCATTTCAAAGCTGTCACTCCTCAAGATATCATCTGTTCTCAGAAGCACACAAGCTTGACACTAAAAGAAGAAGATATAGCTGTTACAAACGTCAAGATTGACTTGGCTCGTGGGAGAGAAAATCCGCTTGAATGCATCAACTTCTATAAGGATTATGATAGTGCAGAGAAGTTTGTGATACCTGAGGACAGAGTCAGTCACTTGTTACCAACAACATATCAAGACATGATAGTGAGAGTATATGCCAAAAAACCAGAATTGGTAGAAGCAGTTTCTGAGGCATTTGAAAACTTCCAAATGAGAACGTATGGAATCAAAGCTCAAGTACACGCAACACCTGAGAAAAAGAAAAGGCGTGTGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HD

Protein Analysis

473

Amino Acids

54.67

Weight (kDa)

6.15

Isoelectric Point (pI)

32.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD PF01966 76 - 186 1.6e-11 HD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 641
AccB7I CCANNNNNTGG 1 cut(s) 298
AccI GTMKAC 2 cut(s) 90, 801
AccII CGCG 1 cut(s) 738
AciI CCGC 3 cut(s) 736, 1034, 1181
AclI AACGTT 1 cut(s) 99
AclWI GGATC 4 cut(s) 116, 666, 679, 950
AcoI YGGCCR 1 cut(s) 364
AcsI RAATTY 5 cut(s) 137, 286, 398, 545, 876
AcuI CTGAAG 1 cut(s) 311
AfaI GTAC 4 cut(s) 219, 541, 1013, 1383
AfiI CCNNNNNNNGG 3 cut(s) 298, 409, 742
AgeI ACCGGT 1 cut(s) 37
AgsI TTSAA 4 cut(s) 683, 1063, 1187, 1343
AjnI CCWGG 3 cut(s) 208, 291, 714
AjuI GAANNNNNNNTTGG 2 cut(s) 668, 700
Alw21I GWGCWC 2 cut(s) 72, 237
Alw26I GTCTC 3 cut(s) 331, 438, 695
AlwI GGATC 4 cut(s) 116, 666, 679, 950
AlwNI CAGNNNCTG 2 cut(s) 215, 1331
Ama87I CYCGRG 2 cut(s) 654, 777
AoxI GGCC 2 cut(s) 364, 371
ApoI RAATTY 5 cut(s) 137, 286, 398, 545, 876
AseI ATTAAT 1 cut(s) 891
AsiGI ACCGGT 1 cut(s) 37
Asp700I GAANNNNTTC 2 cut(s) 402, 578
AspLEI GCGC 2 cut(s) 56, 740
AspS9I GGNCC 1 cut(s) 371
AsuII TTCGAA 1 cut(s) 812
AvaI CYCGRG 2 cut(s) 654, 777
AxyI CCTNAGG 1 cut(s) 1239
BalI TGGCCA 1 cut(s) 366
BamHI GGATCC 1 cut(s) 671
BanII GRGCYC 1 cut(s) 72
BauI CACGAG 1 cut(s) 1164
Bbv12I GWGCWC 2 cut(s) 72, 237
BccI CCATC 1 cut(s) 907
BcgI CGANNNNNNTGC 2 cut(s) 479, 513
BciT130I CCWGG 3 cut(s) 210, 293, 716
BcoDI GTCTC 3 cut(s) 331, 438, 695
BfaI CTAG 3 cut(s) 242, 533, 537
BfoI RGCGCY 1 cut(s) 57
BmcAI AGTACT 1 cut(s) 541
Bme1390I CCNGG 3 cut(s) 210, 293, 716
BmeT110I CYCGRG 2 cut(s) 654, 777
BmgT120I GGNCC 1 cut(s) 371
BmiI GGNNCC 2 cut(s) 673, 1038
BmrFI CCNGG 3 cut(s) 210, 293, 716
BmrI ACTGGG 1 cut(s) 677
BmsI GCATC 3 cut(s) 487, 835, 1200
BmuI ACTGGG 1 cut(s) 677
BpmI CTGGAG 1 cut(s) 936
Bpu14I TTCGAA 1 cut(s) 812
BpuEI CTTGAG 2 cut(s) 1062, 1362
Bsa29I ATCGAT 2 cut(s) 489, 1053
BsaBI GATNNNNATC 2 cut(s) 480, 789
BsaI GGTCTC 1 cut(s) 438
BsaJI CCNNGG 4 cut(s) 292, 367, 741, 849
BsaWI WCCGGW 2 cut(s) 37, 72
Bsc4I CCNNNNNNNGG 3 cut(s) 298, 409, 742
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 2 cut(s) 672, 914
Bse21I CCTNAGG 1 cut(s) 1239
Bse3DI GCAATG 1 cut(s) 571
Bse8I GATNNNNATC 2 cut(s) 480, 789
BseBI CCWGG 3 cut(s) 210, 293, 716
BseCI ATCGAT 2 cut(s) 489, 1053
BseDI CCNNGG 4 cut(s) 292, 367, 741, 849
BseJI GATNNNNATC 2 cut(s) 480, 789
BseLI CCNNNNNNNGG 3 cut(s) 298, 409, 742
BseMI GCAATG 1 cut(s) 571
BseMII CTCAG 6 cut(s) 141, 287, 1109, 1230, 1323, 1386
BseNI ACTGG 2 cut(s) 672, 914
BseRI GAGGAG 2 cut(s) 37, 1065
BsgI GTGCAG 1 cut(s) 1239
Bsh1236I CGCG 1 cut(s) 738
BshFI GGCC 2 cut(s) 366, 373
BshTI ACCGGT 1 cut(s) 37
BshVI ATCGAT 2 cut(s) 489, 1053
BsiHKAI GWGCWC 2 cut(s) 72, 237
BsiHKCI CYCGRG 2 cut(s) 654, 777
BsiSI CCGG 4 cut(s) 38, 51, 59, 73
BslI CCNNNNNNNGG 3 cut(s) 298, 409, 742
BsmAI GTCTC 3 cut(s) 331, 438, 695
BsmBI CGTCTC 1 cut(s) 695
BsmI GAATGC 2 cut(s) 808, 1193
BsnI GGCC 2 cut(s) 366, 373
Bso31I GGTCTC 1 cut(s) 438
BsoBI CYCGRG 2 cut(s) 654, 777
Bsp119I TTCGAA 1 cut(s) 812
Bsp1286I GDGCHC 2 cut(s) 72, 237
Bsp1407I TGTACA 1 cut(s) 217
Bsp143I GATC 6 cut(s) 121, 322, 671, 784, 955, 1054
Bsp19I CCATGG 2 cut(s) 367, 849
BspACI CCGC 3 cut(s) 736, 1034, 1181
BspANI GGCC 2 cut(s) 366, 373
BspCNI CTCAG 6 cut(s) 142, 286, 1108, 1231, 1324, 1387
BspDI ATCGAT 2 cut(s) 489, 1053
BspFNI CGCG 1 cut(s) 738
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 2 cut(s) 673, 1038
BspPI GGATC 4 cut(s) 116, 666, 679, 950
BspT104I TTCGAA 1 cut(s) 812
BspTNI GGTCTC 1 cut(s) 438
BsrDI GCAATG 1 cut(s) 571
BsrFI RCCGGY 1 cut(s) 37
BsrGI TGTACA 1 cut(s) 217
BsrI ACTGG 2 cut(s) 672, 914
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 4 cut(s) 292, 367, 741, 849
BssMI GATC 6 cut(s) 121, 322, 671, 784, 955, 1054
BssSI CACGAG 1 cut(s) 1164
BssT1I CCWWGG 3 cut(s) 367, 741, 849
Bst2BI CACGAG 1 cut(s) 1164
Bst2UI CCWGG 3 cut(s) 210, 293, 716
Bst4CI ACNGT 3 cut(s) 334, 425, 799
BstAUI TGTACA 1 cut(s) 217
BstBI TTCGAA 1 cut(s) 812
BstC8I GCNNGC 3 cut(s) 83, 258, 262
BstDEI CTNAG 8 cut(s) 26, 150, 273, 752, 1095, 1239, 1332, 1395
BstDSI CCRYGG 2 cut(s) 367, 849
BstENI CCTNNNNNAGG 1 cut(s) 407
BstFNI CGCG 1 cut(s) 738
BstH2I RGCGCY 1 cut(s) 57
BstHHI GCGC 2 cut(s) 56, 740
BstKTI GATC 6 cut(s) 124, 325, 674, 787, 958, 1057
BstMAI GTCTC 3 cut(s) 331, 438, 695
BstMBI GATC 6 cut(s) 121, 322, 671, 784, 955, 1054
BstMWI GCNNNNNNNGC 1 cut(s) 779
BstNI CCWGG 3 cut(s) 210, 293, 716
BstNSI RCATGY 1 cut(s) 89
BstSCI CCNGG 3 cut(s) 208, 291, 714
BstUI CGCG 1 cut(s) 738
BstX2I RGATCY 2 cut(s) 671, 955
BstXI CCANNNNNNTGG 1 cut(s) 362
BstYI RGATCY 2 cut(s) 671, 955
Bsu15I ATCGAT 2 cut(s) 489, 1053
Bsu36I CCTNAGG 1 cut(s) 1239
BsuRI GGCC 2 cut(s) 366, 373
BsuTUI ATCGAT 2 cut(s) 489, 1053
BtgI CCRYGG 2 cut(s) 367, 849
BtsIMutI CAGTG 2 cut(s) 147, 434
Cac8I GCNNGC 3 cut(s) 83, 258, 262
CaiI CAGNNNCTG 2 cut(s) 215, 1331
CciI TCATGA 1 cut(s) 436
CfoI GCGC 2 cut(s) 56, 740
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 1 cut(s) 371
ClaI ATCGAT 2 cut(s) 489, 1053
Csp6I GTAC 4 cut(s) 218, 540, 1012, 1382
CspAI ACCGGT 1 cut(s) 37
CviQI GTAC 4 cut(s) 218, 540, 1012, 1382
DdeI CTNAG 8 cut(s) 26, 150, 273, 752, 1095, 1239, 1332, 1395
DpnI GATC 6 cut(s) 123, 324, 673, 786, 957, 1056
DpnII GATC 6 cut(s) 121, 322, 671, 784, 955, 1054
DraI TTTAAA 1 cut(s) 135
DrdI GACNNNNNNGTC 1 cut(s) 641
DseDI GACNNNNNNGTC 1 cut(s) 641
EaeI YGGCCR 1 cut(s) 364
Ecl136II GAGCTC 1 cut(s) 70
Eco130I CCWWGG 3 cut(s) 367, 741, 849
Eco24I GRGCYC 1 cut(s) 72
Eco31I GGTCTC 1 cut(s) 438
Eco32I GATATC 1 cut(s) 1084
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 1 cut(s) 311
Eco81I CCTNAGG 1 cut(s) 1239
Eco88I CYCGRG 2 cut(s) 654, 777
EcoICRI GAGCTC 1 cut(s) 70
EcoNI CCTNNNNNAGG 1 cut(s) 407
EcoRI GAATTC 1 cut(s) 398
EcoRII CCWGG 3 cut(s) 208, 291, 714
EcoRV GATATC 1 cut(s) 1084
EcoT14I CCWWGG 3 cut(s) 367, 741, 849
EcoT22I ATGCAT 1 cut(s) 1193
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 3 cut(s) 367, 741, 849
Esp3I CGTCTC 1 cut(s) 695
FalI AAGNNNNNCTT 4 cut(s) 566, 598, 1142, 1174
FauNDI CATATG 2 cut(s) 384, 468
FblI GTMKAC 2 cut(s) 90, 801
FriOI GRGCYC 1 cut(s) 72
FspBI CTAG 3 cut(s) 242, 533, 537
GlaI GCGC 2 cut(s) 55, 739
GsuI CTGGAG 1 cut(s) 936
HaeII RGCGCY 1 cut(s) 57
HaeIII GGCC 2 cut(s) 366, 373
HapII CCGG 4 cut(s) 38, 51, 59, 73
HhaI GCGC 2 cut(s) 56, 740
Hin6I GCGC 2 cut(s) 54, 738
HinP1I GCGC 2 cut(s) 54, 738
HindIII AAGCTT 1 cut(s) 1108
HinfI GANTC 4 cut(s) 650, 711, 1248, 1368
HpaII CCGG 4 cut(s) 38, 51, 59, 73
Hpy166II GTNNAC 9 cut(s) 35, 91, 173, 454, 625, 665, 760, 802, 1384
Hpy188I TCNGA 6 cut(s) 162, 276, 330, 904, 1098, 1333
Hpy8I GTNNAC 9 cut(s) 35, 91, 173, 454, 625, 665, 760, 802, 1384
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 3 cut(s) 413, 514, 996
HpyCH4III ACNGT 3 cut(s) 334, 425, 799
HpyCH4IV ACGT 3 cut(s) 99, 1146, 1361
HpyCH4V TGCA 6 cut(s) 260, 569, 773, 968, 1191, 1220
HpyF10VI GCNNNNNNNGC 1 cut(s) 779
HpyF3I CTNAG 8 cut(s) 26, 150, 273, 752, 1095, 1239, 1332, 1395
HpySE526I ACGT 3 cut(s) 99, 1146, 1361
HspAI GCGC 2 cut(s) 54, 738
Kzo9I GATC 6 cut(s) 121, 322, 671, 784, 955, 1054
LmnI GCTCC 6 cut(s) 5, 61, 75, 212, 301, 955
LweI GCATC 3 cut(s) 487, 835, 1200
MaeI CTAG 3 cut(s) 242, 533, 537
MaeII ACGT 3 cut(s) 99, 1146, 1361
MaeIII GTNAC 7 cut(s) 190, 419, 1022, 1069, 1138, 1253, 1260
MalI GATC 6 cut(s) 123, 324, 673, 786, 957, 1056
MboI GATC 6 cut(s) 121, 322, 671, 784, 955, 1054
MboII GAAGA 4 cut(s) 317, 873, 1136, 1139
MflI RGATCY 2 cut(s) 671, 955
MhlI GDGCHC 2 cut(s) 72, 237
MlsI TGGCCA 1 cut(s) 366
MluNI TGGCCA 1 cut(s) 366
MlyI GAGTC 2 cut(s) 720, 1257
MnlI CCTC 8 cut(s) 58, 135, 156, 270, 941, 1086, 1234, 1327
Mox20I TGGCCA 1 cut(s) 366
Mph1103I ATGCAT 1 cut(s) 1193
MroXI GAANNNNTTC 2 cut(s) 402, 578
MscI TGGCCA 1 cut(s) 366
MseI TTAA 3 cut(s) 134, 443, 891
MslI CAYNNNNRTG 1 cut(s) 1286
Msp20I TGGCCA 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 556
MspI CCGG 4 cut(s) 38, 51, 59, 73
MspR9I CCNGG 3 cut(s) 210, 293, 716
Mva1269I GAATGC 2 cut(s) 808, 1193
MvaI CCWGG 3 cut(s) 210, 293, 716
MvnI CGCG 1 cut(s) 738
MwoI GCNNNNNNNGC 1 cut(s) 779
NcoI CCATGG 2 cut(s) 367, 849
NdeI CATATG 2 cut(s) 384, 468
NdeII GATC 6 cut(s) 121, 322, 671, 784, 955, 1054
NlaIV GGNNCC 2 cut(s) 673, 1038
NmuCI GTSAC 4 cut(s) 190, 419, 1069, 1253
NsiI ATGCAT 1 cut(s) 1193
NspI RCATGY 1 cut(s) 89
NspV TTCGAA 1 cut(s) 812
PaeR7I CTCGAG 2 cut(s) 654, 777
PagI TCATGA 1 cut(s) 436
PctI GAATGC 2 cut(s) 808, 1193
PdmI GAANNNNTTC 2 cut(s) 402, 578
PfeI GAWTC 2 cut(s) 650, 1368
PflFI GACNNNGTC 1 cut(s) 1247
PflMI CCANNNNNTGG 1 cut(s) 298
PfoI TCCNGGA 2 cut(s) 208, 714
PinAI ACCGGT 1 cut(s) 37
PleI GAGTC 2 cut(s) 719, 1256
PpsI GAGTC 2 cut(s) 719, 1256
PshBI ATTAAT 1 cut(s) 891
Psp124BI GAGCTC 1 cut(s) 72
Psp1406I AACGTT 1 cut(s) 99
Psp6I CCWGG 3 cut(s) 208, 291, 714
PspGI CCWGG 3 cut(s) 208, 291, 714
PspN4I GGNNCC 2 cut(s) 673, 1038
PspPI GGNCC 1 cut(s) 371
PspXI VCTCGAGB 1 cut(s) 777
PstNI CAGNNNCTG 2 cut(s) 215, 1331
PsuI RGATCY 2 cut(s) 671, 955
PsyI GACNNNGTC 1 cut(s) 1247
PvuII CAGCTG 1 cut(s) 556
RsaI GTAC 4 cut(s) 219, 541, 1013, 1383
RsaNI GTAC 4 cut(s) 218, 540, 1012, 1382
RseI CAYNNNNRTG 1 cut(s) 1286
SacI GAGCTC 1 cut(s) 72
SaqAI TTAA 3 cut(s) 134, 443, 891
Sau3AI GATC 6 cut(s) 121, 322, 671, 784, 955, 1054
Sau96I GGNCC 1 cut(s) 371
ScaI AGTACT 1 cut(s) 541
SchI GAGTC 2 cut(s) 720, 1257
ScrFI CCNGG 3 cut(s) 210, 293, 716
SduI GDGCHC 2 cut(s) 72, 237
SfaNI GCATC 3 cut(s) 487, 835, 1200
Sfr274I CTCGAG 2 cut(s) 654, 777
SfuI TTCGAA 1 cut(s) 812
SlaI CTCGAG 2 cut(s) 654, 777
SmiMI CAYNNNNRTG 1 cut(s) 1286
SmlI CTYRAG 4 cut(s) 654, 777, 1077, 1377
SmoI CTYRAG 4 cut(s) 654, 777, 1077, 1377
SsiI CCGC 3 cut(s) 736, 1034, 1181
SspMI CTAG 3 cut(s) 242, 533, 537
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 3 cut(s) 208, 291, 714
StyI CCWWGG 3 cut(s) 367, 741, 849
TaaI ACNGT 3 cut(s) 334, 425, 799
TaiI ACGT 3 cut(s) 102, 1149, 1364
TatI WGTACW 3 cut(s) 217, 539, 1381
TfiI GAWTC 2 cut(s) 650, 1368
Tru1I TTAA 3 cut(s) 134, 443, 891
Tru9I TTAA 3 cut(s) 134, 443, 891
TscAI CASTG 2 cut(s) 154, 434
TseFI GTSAC 4 cut(s) 190, 419, 1069, 1253
Tsp45I GTSAC 4 cut(s) 190, 419, 1069, 1253
TspDTI ATGAA 3 cut(s) 92, 130, 738
TspGWI ACGGA 2 cut(s) 635, 980
TspRI CASTG 2 cut(s) 154, 434
Tth111I GACNNNGTC 1 cut(s) 1247
Van91I CCANNNNNTGG 1 cut(s) 298
VspI ATTAAT 1 cut(s) 891
XagI CCTNNNNNAGG 1 cut(s) 407
XapI RAATTY 5 cut(s) 137, 286, 398, 545, 876
XceI RCATGY 1 cut(s) 89
XcmI CCANNNNNNNNNTGG 2 cut(s) 868, 906
XhoI CTCGAG 2 cut(s) 654, 777
XmiI GTMKAC 2 cut(s) 90, 801
XmnI GAANNNNTTC 2 cut(s) 402, 578
XspI CTAG 3 cut(s) 242, 533, 537
ZrmI AGTACT 1 cut(s) 541
Zsp2I ATGCAT 1 cut(s) 1193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.