Rh4AG408800

4Fe-4S single cluster domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002219
Physical Location & Seq
Forward (+)
70897842 .. 70903263
5422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG408800.1

Sequence Viewer

Length: 1017 bp
ATGGCGGCTATGGCGAACGACTTTCAGGATCGCCGGAATTTGAAGCTAGTTCACGATAACGTTCACGGCATCGTCTACCTCGAGCCGCTCTCCTTGAATTTTATTGACACTGAACAGTTTCAGAGGCTTCGTGATCTGAAGCAACTTGGTCTGACCAGCATGGTTTACCCTGGAGCTGTACATTCTAGATTTGAGCATTCACTTGGGGTATATTGGCTTGCTGGTGAAGCTATCAATAAAATCAAAACTTACCAAGGCGAGGAGCTTGGAATTGACAGCTTTGATGTGAGAACAGTAAAACTTGCTGGACTACTGCATGATATTGGCCATGGGCCTTTCAGCCACATGTTTGAGAGCGCGTTTCTTCCTCGGGTTCATTCTGGTGACAAATGGTTCCATGAGGATATGTCTGTGAAGATGATTGACTACATTGTCGACGAGCACCACATTGACATGGATTCTGAAGAGCTAAAGAGAGTGAAGGACCTGATCCTGGCTAGCTCTAAACATGTGGCACAGAATGCTGCACCAGAAAAGCGGTTCTTGTATGATATTGTTGCTAATGGACGCACTGGAATAGATGTTGACAAGTTTGATTACATTGAACGTGACTCCCGTGCCTGTGGCATAGGCTGTAATTTTCACTTTGAGAGGCTGATGGAAACCATGCGAGTTATGGAGGATGAGATATGTTACCGTGCTAGTGAATATCTGACAATCAACAAGCTGTTTATGACTCGGGCTAATCTGCATCGGACTGTCTATACGCATGCAAAAGTGAAGGACTATGAGAGTACTGATAAATTCCGAATCCAAGTTGATGGTGTAAGTCACTTGCTTCCTGCATTTTATCAAGACAAGATAGTCAGGGTCTACTCCAAGAAGCCGAACCTGGTTGCAGCAGTTGCAGATGCTTTTGAGAATTATCAGCTGAAGACATATGGGAGGATGGTACAAGTACATTCAACTCCGGAGAAAAAGAAAAAAAGAACTGCACGAAGCTTAGCGCTGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer4_14 HD Radical_SAM

Protein Analysis

338

Amino Acids

39.08

Weight (kDa)

7.0

Isoelectric Point (pI)

30.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD PF01966 63 - 155 3.2e-11 HD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 431, 863
AccBSI CCGCTC 1 cut(s) 88
AccI GTMKAC 3 cut(s) 75, 435, 873
AccII CGCG 1 cut(s) 359
AccIII TCCGGA 1 cut(s) 970
AciI CCGC 3 cut(s) 5, 86, 538
AclI AACGTT 1 cut(s) 60
AclWI GGATC 2 cut(s) 36, 484
AcoI YGGCCR 1 cut(s) 325
AcsI RAATTY 3 cut(s) 37, 97, 803
AcuI CTGAAG 3 cut(s) 158, 483, 953
AfaI GTAC 4 cut(s) 180, 796, 954, 960
AfeI AGCGCT 1 cut(s) 1008
AfiI CCNNNNNNNGG 2 cut(s) 259, 493
AflIII ACRYGT 2 cut(s) 345, 508
AgsI TTSAA 4 cut(s) 43, 97, 605, 966
AjnI CCWGG 3 cut(s) 169, 492, 891
Alw21I GWGCWC 1 cut(s) 444
AlwI GGATC 2 cut(s) 36, 484
Ama87I CYCGRG 3 cut(s) 80, 369, 738
Aor13HI TCCGGA 1 cut(s) 970
Aor51HI AGCGCT 1 cut(s) 1008
AoxI GGCC 2 cut(s) 325, 332
ApeKI GCWGC 3 cut(s) 524, 899, 1009
ApoI RAATTY 3 cut(s) 37, 97, 803
Asp700I GAANNNNTTC 1 cut(s) 117
AspLEI GCGC 2 cut(s) 359, 1009
AspS9I GGNCC 2 cut(s) 332, 484
AsuHPI GGTGA 2 cut(s) 236, 395
AsuNHI GCTAGC 1 cut(s) 497
AvaI CYCGRG 3 cut(s) 80, 369, 738
AvaII GGWCC 1 cut(s) 484
BalI TGGCCA 1 cut(s) 327
BbsI GAAGAC 1 cut(s) 941
Bbv12I GWGCWC 1 cut(s) 444
BbvI GCAGC 3 cut(s) 511, 911, 996
BccI CCATC 3 cut(s) 652, 815, 943
BceAI ACGGC 1 cut(s) 82
BciT130I CCWGG 3 cut(s) 171, 494, 893
BfaI CTAG 4 cut(s) 47, 186, 498, 702
BfmI CTRYAG 1 cut(s) 1010
BfoI RGCGCY 1 cut(s) 1010
BisI GCNGC 5 cut(s) 6, 86, 525, 900, 1010
BlpI GCTNAGC 1 cut(s) 1003
BlsI GCNGC 5 cut(s) 7, 87, 526, 901, 1011
BmcAI AGTACT 1 cut(s) 796
Bme1390I CCNGG 3 cut(s) 171, 494, 893
Bme18I GGWCC 1 cut(s) 484
BmeT110I CYCGRG 3 cut(s) 80, 369, 738
BmgT120I GGNCC 2 cut(s) 332, 484
BmiI GGNNCC 1 cut(s) 395
BmrFI CCNGG 3 cut(s) 171, 494, 893
BmsI GCATC 3 cut(s) 78, 760, 901
BmtI GCTAGC 1 cut(s) 501
BpiI GAAGAC 1 cut(s) 941
BplI GAGNNNNNCTC 2 cut(s) 74, 106
BpmI CTGGAG 1 cut(s) 192
Bpu1102I GCTNAGC 1 cut(s) 1003
BsaJI CCNNGG 4 cut(s) 169, 253, 328, 368
BsaWI WCCGGW 1 cut(s) 970
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 493
Bse1I ACTGG 1 cut(s) 577
BseAI TCCGGA 1 cut(s) 970
BseBI CCWGG 3 cut(s) 171, 494, 893
BseDI CCNNGG 4 cut(s) 169, 253, 328, 368
BseGI GGATG 2 cut(s) 688, 954
BseLI CCNNNNNNNGG 2 cut(s) 259, 493
BseNI ACTGG 1 cut(s) 577
BseRI GAGGAG 1 cut(s) 275
BseXI GCAGC 3 cut(s) 511, 911, 996
BsgI GTGCAG 2 cut(s) 510, 978
Bsh1236I CGCG 1 cut(s) 359
BshFI GGCC 2 cut(s) 327, 334
BsiHKAI GWGCWC 1 cut(s) 444
BsiHKCI CYCGRG 3 cut(s) 80, 369, 738
BsiSI CCGG 2 cut(s) 34, 971
BslI CCNNNNNNNGG 2 cut(s) 259, 493
BsmI GAATGC 2 cut(s) 196, 526
BsnI GGCC 2 cut(s) 327, 334
BsoBI CYCGRG 3 cut(s) 80, 369, 738
Bsp1286I GDGCHC 1 cut(s) 444
Bsp13I TCCGGA 1 cut(s) 970
Bsp1407I TGTACA 1 cut(s) 178
Bsp143I GATC 3 cut(s) 28, 133, 489
Bsp1720I GCTNAGC 1 cut(s) 1003
Bsp19I CCATGG 1 cut(s) 328
BspACI CCGC 3 cut(s) 5, 86, 538
BspANI GGCC 2 cut(s) 327, 334
BspEI TCCGGA 1 cut(s) 970
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 1 cut(s) 395
BspMAI CTGCAG 1 cut(s) 1014
BspOI GCTAGC 1 cut(s) 501
BspPI GGATC 2 cut(s) 36, 484
BspQI GCTCTTC 1 cut(s) 459
BsrBI CCGCTC 1 cut(s) 88
BsrGI TGTACA 1 cut(s) 178
BsrI ACTGG 1 cut(s) 577
BssECI CCNNGG 4 cut(s) 169, 253, 328, 368
BssMI GATC 3 cut(s) 28, 133, 489
BssT1I CCWWGG 2 cut(s) 253, 328
Bst2UI CCWGG 3 cut(s) 171, 494, 893
Bst4CI ACNGT 4 cut(s) 117, 295, 698, 760
Bst6I CTCTTC 1 cut(s) 459
BstAPI GCANNNNNTGC 2 cut(s) 521, 905
BstAUI TGTACA 1 cut(s) 178
BstC8I GCNNGC 3 cut(s) 219, 499, 771
BstDEI CTNAG 1 cut(s) 1003
BstDSI CCRYGG 1 cut(s) 328
BstF5I GGATG 2 cut(s) 688, 954
BstFNI CGCG 1 cut(s) 359
BstH2I RGCGCY 1 cut(s) 1010
BstHHI GCGC 2 cut(s) 359, 1009
BstKTI GATC 3 cut(s) 31, 136, 492
BstMBI GATC 3 cut(s) 28, 133, 489
BstMWI GCNNNNNNNGC 4 cut(s) 11, 227, 521, 905
BstNI CCWGG 3 cut(s) 171, 494, 893
BstNSI RCATGY 3 cut(s) 349, 512, 773
BstSCI CCNGG 3 cut(s) 169, 492, 891
BstSFI CTRYAG 1 cut(s) 1010
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 3 cut(s) 511, 911, 996
BstV2I GAAGAC 1 cut(s) 941
BstXI CCANNNNNNTGG 1 cut(s) 821
BsuRI GGCC 2 cut(s) 327, 334
BtgI CCRYGG 1 cut(s) 328
BtsCI GGATG 2 cut(s) 688, 954
BtsIMutI CAGTG 2 cut(s) 108, 570
Cac8I GCNNGC 3 cut(s) 219, 499, 771
CfoI GCGC 2 cut(s) 359, 1009
Cfr13I GGNCC 2 cut(s) 332, 484
CseI GACGC 1 cut(s) 576
CsiI ACCWGGT 1 cut(s) 891
Csp6I GTAC 4 cut(s) 179, 795, 953, 959
CviAII CATG 9 cut(s) 160, 317, 329, 346, 398, 454, 509, 667, 770
CviQI GTAC 4 cut(s) 179, 795, 953, 959
DdeI CTNAG 1 cut(s) 1003
DpnI GATC 3 cut(s) 30, 135, 491
DpnII GATC 3 cut(s) 28, 133, 489
DrdI GACNNNNNNGTC 2 cut(s) 431, 863
DseDI GACNNNNNNGTC 2 cut(s) 431, 863
EaeI YGGCCR 1 cut(s) 325
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
Eco130I CCWWGG 2 cut(s) 253, 328
Eco47I GGWCC 1 cut(s) 484
Eco47III AGCGCT 1 cut(s) 1008
Eco57I CTGAAG 3 cut(s) 158, 483, 953
Eco88I CYCGRG 3 cut(s) 80, 369, 738
EcoO109I RGGNCCY 1 cut(s) 484
EcoRII CCWGG 3 cut(s) 169, 492, 891
EcoT14I CCWWGG 2 cut(s) 253, 328
ErhI CCWWGG 2 cut(s) 253, 328
FaeI CATG 9 cut(s) 163, 320, 332, 349, 401, 457, 512, 670, 773
FalI AAGNNNNNCTT 2 cut(s) 527, 559
FatI CATG 9 cut(s) 159, 316, 328, 345, 397, 453, 508, 666, 769
FauNDI CATATG 1 cut(s) 940
FblI GTMKAC 3 cut(s) 75, 435, 873
Fnu4HI GCNGC 5 cut(s) 6, 86, 525, 900, 1010
FokI GGATG 2 cut(s) 695, 961
Fsp4HI GCNGC 5 cut(s) 6, 86, 525, 900, 1010
FspBI CTAG 4 cut(s) 47, 186, 498, 702
GlaI GCGC 2 cut(s) 358, 1008
GluI GCNGC 5 cut(s) 6, 86, 525, 900, 1010
GsuI CTGGAG 1 cut(s) 192
HaeII RGCGCY 1 cut(s) 1010
HaeIII GGCC 2 cut(s) 327, 334
HapII CCGG 2 cut(s) 34, 971
HgaI GACGC 1 cut(s) 576
HhaI GCGC 2 cut(s) 359, 1009
Hin1II CATG 9 cut(s) 163, 320, 332, 349, 401, 457, 512, 670, 773
Hin6I GCGC 2 cut(s) 357, 1007
HinP1I GCGC 2 cut(s) 357, 1007
HincII GTYRAC 2 cut(s) 436, 586
HindII GTYRAC 2 cut(s) 436, 586
HindIII AAGCTT 1 cut(s) 1000
HinfI GANTC 4 cut(s) 458, 611, 736, 810
HpaII CCGG 2 cut(s) 34, 971
HphI GGTGA 2 cut(s) 236, 395
Hpy166II GTNNAC 7 cut(s) 52, 64, 76, 166, 436, 586, 874
Hpy188I TCNGA 7 cut(s) 123, 138, 153, 463, 714, 756, 809
Hpy188III TCNNGA 6 cut(s) 26, 53, 131, 186, 854, 971
Hpy8I GTNNAC 7 cut(s) 52, 64, 76, 166, 436, 586, 874
Hpy99I CGWCG 1 cut(s) 440
HpyAV CCTTC 2 cut(s) 475, 775
HpyCH4III ACNGT 4 cut(s) 117, 295, 698, 760
HpyCH4IV ACGT 2 cut(s) 60, 607
HpyCH4V TGCA 9 cut(s) 316, 527, 751, 773, 845, 899, 908, 995, 1012
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 227, 521, 905
HpyF3I CTNAG 1 cut(s) 1003
HpySE526I ACGT 2 cut(s) 60, 607
Hsp92II CATG 9 cut(s) 163, 320, 332, 349, 401, 457, 512, 670, 773
HspAI GCGC 2 cut(s) 357, 1007
Kpn2I TCCGGA 1 cut(s) 970
Kzo9I GATC 3 cut(s) 28, 133, 489
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 2 cut(s) 173, 262
Lsp1109I GCAGC 3 cut(s) 511, 911, 996
LweI GCATC 3 cut(s) 78, 760, 901
MabI ACCWGGT 1 cut(s) 891
MaeI CTAG 4 cut(s) 47, 186, 498, 702
MaeII ACGT 2 cut(s) 60, 607
MaeIII GTNAC 4 cut(s) 383, 608, 692, 830
MalI GATC 3 cut(s) 30, 135, 491
MbiI CCGCTC 1 cut(s) 88
MboI GATC 3 cut(s) 28, 133, 489
MboII GAAGA 4 cut(s) 356, 427, 476, 946
MhlI GDGCHC 1 cut(s) 444
MlsI TGGCCA 1 cut(s) 327
MluCI AATT 6 cut(s) 37, 97, 270, 637, 803, 922
MluNI TGGCCA 1 cut(s) 327
MlyI GAGTC 2 cut(s) 605, 730
MnlI CCTC 8 cut(s) 89, 117, 253, 378, 394, 645, 673, 939
Mox20I TGGCCA 1 cut(s) 327
MroI TCCGGA 1 cut(s) 970
MroXI GAANNNNTTC 1 cut(s) 117
MscI TGGCCA 1 cut(s) 327
MslI CAYNNNNRTG 2 cut(s) 381, 452
Msp20I TGGCCA 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 931
MspI CCGG 2 cut(s) 34, 971
MspR9I CCNGG 3 cut(s) 171, 494, 893
Mva1269I GAATGC 2 cut(s) 196, 526
MvaI CCWGG 3 cut(s) 171, 494, 893
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 4 cut(s) 11, 227, 521, 905
NcoI CCATGG 1 cut(s) 328
NdeI CATATG 1 cut(s) 940
NdeII GATC 3 cut(s) 28, 133, 489
NheI GCTAGC 1 cut(s) 497
NlaIII CATG 9 cut(s) 163, 320, 332, 349, 401, 457, 512, 670, 773
NlaIV GGNNCC 1 cut(s) 395
NmuCI GTSAC 3 cut(s) 383, 608, 830
NspI RCATGY 3 cut(s) 349, 512, 773
PaeI GCATGC 1 cut(s) 773
PaeR7I CTCGAG 1 cut(s) 80
PciI ACATGT 2 cut(s) 345, 508
PciSI GCTCTTC 1 cut(s) 459
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 613
PctI GAATGC 2 cut(s) 196, 526
PdmI GAANNNNTTC 1 cut(s) 117
PfeI GAWTC 2 cut(s) 458, 810
PkrI GCNGC 5 cut(s) 7, 87, 526, 901, 1011
PleI GAGTC 2 cut(s) 605, 730
PpsI GAGTC 2 cut(s) 605, 730
PpuMI RGGWCCY 1 cut(s) 484
PscI ACATGT 2 cut(s) 345, 508
Psp1406I AACGTT 1 cut(s) 60
Psp5II RGGWCCY 1 cut(s) 484
Psp6I CCWGG 3 cut(s) 169, 492, 891
PspGI CCWGG 3 cut(s) 169, 492, 891
PspN4I GGNNCC 1 cut(s) 395
PspPI GGNCC 2 cut(s) 332, 484
PspPPI RGGWCCY 1 cut(s) 484
PspXI VCTCGAGB 1 cut(s) 80
PstI CTGCAG 1 cut(s) 1014
PvuII CAGCTG 1 cut(s) 931
RsaI GTAC 4 cut(s) 180, 796, 954, 960
RsaNI GTAC 4 cut(s) 179, 795, 953, 959
RseI CAYNNNNRTG 2 cut(s) 381, 452
SalI GTCGAC 1 cut(s) 434
SapI GCTCTTC 1 cut(s) 459
SatI GCNGC 5 cut(s) 6, 86, 525, 900, 1010
Sau3AI GATC 3 cut(s) 28, 133, 489
Sau96I GGNCC 2 cut(s) 332, 484
ScaI AGTACT 1 cut(s) 796
SchI GAGTC 2 cut(s) 605, 730
ScrFI CCNGG 3 cut(s) 171, 494, 893
SduI GDGCHC 1 cut(s) 444
SexAI ACCWGGT 1 cut(s) 891
SfaNI GCATC 3 cut(s) 78, 760, 901
SfcI CTRYAG 1 cut(s) 1010
Sfr274I CTCGAG 1 cut(s) 80
SinI GGWCC 1 cut(s) 484
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 2 cut(s) 381, 452
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
SphI GCATGC 1 cut(s) 773
Sse9I AATT 6 cut(s) 37, 97, 270, 637, 803, 922
SsiI CCGC 3 cut(s) 5, 86, 538
SspMI CTAG 4 cut(s) 47, 186, 498, 702
StyD4I CCNGG 3 cut(s) 169, 492, 891
StyI CCWWGG 2 cut(s) 253, 328
TaaI ACNGT 4 cut(s) 117, 295, 698, 760
TaiI ACGT 2 cut(s) 63, 610
TaqI TCGA 2 cut(s) 81, 435
TasI AATT 6 cut(s) 37, 97, 270, 637, 803, 922
TatI WGTACW 3 cut(s) 178, 794, 958
TauI GCSGC 2 cut(s) 8, 88
TfiI GAWTC 2 cut(s) 458, 810
TscAI CASTG 2 cut(s) 115, 577
TseFI GTSAC 3 cut(s) 383, 608, 830
TseI GCWGC 3 cut(s) 524, 899, 1009
Tsp45I GTSAC 3 cut(s) 383, 608, 830
TspDTI ATGAA 1 cut(s) 365
TspRI CASTG 2 cut(s) 115, 577
VpaK11BI GGWCC 1 cut(s) 484
XapI RAATTY 3 cut(s) 37, 97, 803
XbaI TCTAGA 1 cut(s) 185
XceI RCATGY 3 cut(s) 349, 512, 773
XcmI CCANNNNNNNNNTGG 1 cut(s) 673
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 3 cut(s) 75, 435, 873
XmnI GAANNNNTTC 1 cut(s) 117
XspI CTAG 4 cut(s) 47, 186, 498, 702
ZrmI AGTACT 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.